Journal of immunology (Baltimore, Md. : 1950), Jan 30, 2015
The human Ig repertoire is vast, producing billions of unique Abs from a limited number of germli... more The human Ig repertoire is vast, producing billions of unique Abs from a limited number of germline Ig genes. The IgH V region (IGHV) is central to Ag binding and consists of 48 functional genes. In this study, we analyzed whether HIV-1-infected individuals who develop broadly neutralizing Abs show a distinctive germline IGHV profile. Using both 454 and Illumina technologies, we sequenced the IGHV repertoire of 28 HIV-infected South African women from the Centre for the AIDS Programme of Research in South Africa (CAPRISA) 002 and 004 cohorts, 13 of whom developed broadly neutralizing Abs. Of the 259 IGHV alleles identified in this study, approximately half were not found in the International Immunogenetics Database (IMGT). This included 85 entirely novel alleles and 38 alleles that matched rearranged sequences in non-IMGT databases. Analysis of the rearranged H chain V region genes of mAbs isolated from seven of these women, as well as previously isolated broadly neutralizing Abs fr...
Early initiation of antiretroviral therapy reduces HIV-related infant mortality. The early peak o... more Early initiation of antiretroviral therapy reduces HIV-related infant mortality. The early peak of pediatric HIV-related deaths in South Africa occurs at three months of age, coinciding with the earliest age at which treatment is initiated following PCR testing at 6-weeks of age. Earlier diagnosis is necessary to reduce infant mortality. The performance of the Amplicor DNA PCR, COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) and Aptima assays for detecting early HIV-infection (acquired in utero and intrapartum) up to 6-weeks of age was compared. Dried blood spots (DBS) were collected at birth, 2-, 4- and 6-weeks from HIV-exposed infants enrolled in an observational, cohort study in Johannesburg, South Africa. HIV-status was determined at 6-weeks by DNA PCR on whole blood. Serial DBS samples from all HIV-infected infants and two HIV-uninfected, age-matched controls were tested with the 3 assays. Of 710 infants of known HIV-status, 38 (5.4%) had in utero (n=29) or intrapartum (n=9) infections....
Journal of immunology (Baltimore, Md. : 1950), Jan 30, 2015
The human Ig repertoire is vast, producing billions of unique Abs from a limited number of germli... more The human Ig repertoire is vast, producing billions of unique Abs from a limited number of germline Ig genes. The IgH V region (IGHV) is central to Ag binding and consists of 48 functional genes. In this study, we analyzed whether HIV-1-infected individuals who develop broadly neutralizing Abs show a distinctive germline IGHV profile. Using both 454 and Illumina technologies, we sequenced the IGHV repertoire of 28 HIV-infected South African women from the Centre for the AIDS Programme of Research in South Africa (CAPRISA) 002 and 004 cohorts, 13 of whom developed broadly neutralizing Abs. Of the 259 IGHV alleles identified in this study, approximately half were not found in the International Immunogenetics Database (IMGT). This included 85 entirely novel alleles and 38 alleles that matched rearranged sequences in non-IMGT databases. Analysis of the rearranged H chain V region genes of mAbs isolated from seven of these women, as well as previously isolated broadly neutralizing Abs fr...
Early initiation of antiretroviral therapy reduces HIV-related infant mortality. The early peak o... more Early initiation of antiretroviral therapy reduces HIV-related infant mortality. The early peak of pediatric HIV-related deaths in South Africa occurs at three months of age, coinciding with the earliest age at which treatment is initiated following PCR testing at 6-weeks of age. Earlier diagnosis is necessary to reduce infant mortality. The performance of the Amplicor DNA PCR, COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) and Aptima assays for detecting early HIV-infection (acquired in utero and intrapartum) up to 6-weeks of age was compared. Dried blood spots (DBS) were collected at birth, 2-, 4- and 6-weeks from HIV-exposed infants enrolled in an observational, cohort study in Johannesburg, South Africa. HIV-status was determined at 6-weeks by DNA PCR on whole blood. Serial DBS samples from all HIV-infected infants and two HIV-uninfected, age-matched controls were tested with the 3 assays. Of 710 infants of known HIV-status, 38 (5.4%) had in utero (n=29) or intrapartum (n=9) infections....
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Papers by L. Berrie