A rhabdovirus infecting maize and wheat crops in Argentina was molecularly characterized. Through... more A rhabdovirus infecting maize and wheat crops in Argentina was molecularly characterized. Through next-generation sequencing (NGS) of symptomatic leaf samples, the complete genome was obtained of two isolates of maize yellow striate virus (MYSV), a putative new rhabdovirus, differing by only 0.4% at the nucleotide level. The MYSV genome consists of 12,654 nucleotides for maize and wheat virus isolates, and shares 71% nucleotide sequence identity with the complete genome of barley yellow striate mosaic virus (BYSMV, NC028244). Ten open reading frames (ORFs) were predicted in the MYSV genome from the antigenomic strand and were compared with their BYSMV counterparts. The highest amino acid sequence identity of the MYSV and BYSMV proteins was 80% between the L proteins, and the lowest was 37% between the proteins 4. Phylogenetic analysis suggested that the MYSV isolates are new members of the genus Cytorhabdovirus, family Rhabdoviridae. Yellow striate, affecting maize and wheat crops i...
La presencia del "Corn stunt spiroplasma" (Spiroplasma kunkelii Whitcomb) (CSS) en el N... more La presencia del "Corn stunt spiroplasma" (Spiroplasma kunkelii Whitcomb) (CSS) en el Noroeste Argentino y la falta de información acerca de sus efectos en la producción maicera regional indujeron a realizar esta primera cuantificación de los daños ocasionados por la enfermedad. Durante las campañas 1999/2000 y 2001/2002, la misma se detectó mediante observación de síntomas típicos y se confirmó mediante la técnica de NC-ELISA. Se determinó la presencia de CSS en todos los lotes de maíz prospectados en 8 localidades de Tucumán; los niveles de incidencia, obtenidos por serología, variaron entre 12 y 62%. Se determinó que la enfermedad disminuye en promedio el 70% de la producción, con un rango entre 50 y 90% en función de la intensidad de la reacción serológica del grupo de plantas evaluadas; y en plantas severamente afectadas la producción puede ser nula. Estos resultados preliminares demuestran que el CSS es un factor limitante de la producción maicera en la región, y est...
Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al, 2007
Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the m... more Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus (SPMSV). Both diseases cause a synergism between the potyviruses (SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in the infected tissues. In single infections, virions of the crinivirus genus are limited to the phloem while potyviral virions are found in most tissues of the infected plant. The purpose of this work was to localize the heat shock protein 70 homolog (HSP70h), a movement protein for genus crinivirus, of an Argentinean SPCSV ...
ABSTRACT The objective of this work was to evaluate the occurence and yield losses by corn stunti... more ABSTRACT The objective of this work was to evaluate the occurence and yield losses by corn stunting diseases and maize viruses in "safrinha" season. Disease diagnostics based on plant Symptoms were confirmed by PCR or RT-PCR assays. Insect samples were collected in 24 fields for identification of vectors of the pathogens. Corn stunting diseases symptoms were observed in all crops evaluated, with incidence levels ranging from 6.2% to 49.9% (average 20.7%) and the presence of the leafhopper Dalbulus maidis, was detected in 20 of the 24 areas evaluated. This insect species was prevalent, representing 66,6% of total leafhoppers specimens collected. The potential yield losses caused by mollicutes was estimated around US$ 16.5 million. Few plants showing Maize rayado fino virus and Maize dwarf mosaic virus symptoms were found and virus infection was confirmed by RT-PCR. Mal de Rio Cuarto virus was not detected in all 441 plants analyzed by DAS-ELISA. Results of PCR assays indicated the presence of a possible phytoplasma different from maize bushy stunt phytoplasma in two stunting maize plants showing leaves distortions and grain in the inflorescence. However, new studies should be done to confirm the identity of this possible new phytoplasma.
A rhabdovirus infecting maize and wheat crops in Argentina was molecularly characterized. Through... more A rhabdovirus infecting maize and wheat crops in Argentina was molecularly characterized. Through next-generation sequencing (NGS) of symptomatic leaf samples, the complete genome was obtained of two isolates of maize yellow striate virus (MYSV), a putative new rhabdovirus, differing by only 0.4% at the nucleotide level. The MYSV genome consists of 12,654 nucleotides for maize and wheat virus isolates, and shares 71% nucleotide sequence identity with the complete genome of barley yellow striate mosaic virus (BYSMV, NC028244). Ten open reading frames (ORFs) were predicted in the MYSV genome from the antigenomic strand and were compared with their BYSMV counterparts. The highest amino acid sequence identity of the MYSV and BYSMV proteins was 80% between the L proteins, and the lowest was 37% between the proteins 4. Phylogenetic analysis suggested that the MYSV isolates are new members of the genus Cytorhabdovirus, family Rhabdoviridae. Yellow striate, affecting maize and wheat crops i...
La presencia del "Corn stunt spiroplasma" (Spiroplasma kunkelii Whitcomb) (CSS) en el N... more La presencia del "Corn stunt spiroplasma" (Spiroplasma kunkelii Whitcomb) (CSS) en el Noroeste Argentino y la falta de información acerca de sus efectos en la producción maicera regional indujeron a realizar esta primera cuantificación de los daños ocasionados por la enfermedad. Durante las campañas 1999/2000 y 2001/2002, la misma se detectó mediante observación de síntomas típicos y se confirmó mediante la técnica de NC-ELISA. Se determinó la presencia de CSS en todos los lotes de maíz prospectados en 8 localidades de Tucumán; los niveles de incidencia, obtenidos por serología, variaron entre 12 y 62%. Se determinó que la enfermedad disminuye en promedio el 70% de la producción, con un rango entre 50 y 90% en función de la intensidad de la reacción serológica del grupo de plantas evaluadas; y en plantas severamente afectadas la producción puede ser nula. Estos resultados preliminares demuestran que el CSS es un factor limitante de la producción maicera en la región, y est...
Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al, 2007
Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the m... more Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus (SPMSV). Both diseases cause a synergism between the potyviruses (SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in the infected tissues. In single infections, virions of the crinivirus genus are limited to the phloem while potyviral virions are found in most tissues of the infected plant. The purpose of this work was to localize the heat shock protein 70 homolog (HSP70h), a movement protein for genus crinivirus, of an Argentinean SPCSV ...
ABSTRACT The objective of this work was to evaluate the occurence and yield losses by corn stunti... more ABSTRACT The objective of this work was to evaluate the occurence and yield losses by corn stunting diseases and maize viruses in "safrinha" season. Disease diagnostics based on plant Symptoms were confirmed by PCR or RT-PCR assays. Insect samples were collected in 24 fields for identification of vectors of the pathogens. Corn stunting diseases symptoms were observed in all crops evaluated, with incidence levels ranging from 6.2% to 49.9% (average 20.7%) and the presence of the leafhopper Dalbulus maidis, was detected in 20 of the 24 areas evaluated. This insect species was prevalent, representing 66,6% of total leafhoppers specimens collected. The potential yield losses caused by mollicutes was estimated around US$ 16.5 million. Few plants showing Maize rayado fino virus and Maize dwarf mosaic virus symptoms were found and virus infection was confirmed by RT-PCR. Mal de Rio Cuarto virus was not detected in all 441 plants analyzed by DAS-ELISA. Results of PCR assays indicated the presence of a possible phytoplasma different from maize bushy stunt phytoplasma in two stunting maize plants showing leaves distortions and grain in the inflorescence. However, new studies should be done to confirm the identity of this possible new phytoplasma.
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