Environmental science and pollution research international, Jan 13, 2015
This study successfully applied an improved valvometry technique to measure waterborne copper (Cu... more This study successfully applied an improved valvometry technique to measure waterborne copper (Cu), based on valve activity dynamics of the freshwater clam Corbicula fluminea. The improved valvometry technique allows the use of free-range bivalves and avoids causing stresses from experimental artifacts. The proposed daily valve rhythm models and a toxicodynamics-based Hill model were linked to predict valve dynamic responses under different Cu exposures with a circadian valve rhythm endpoint. Cu-specific detection threshold was 5.6 (95 % CI 2.1-9.3) and 19.5 (14.6-24.3) μg L(-1) for C. fluminea, based on response times of 300 and 30 min, respectively. Upon exposure to Cu concentrations in excess of 50 μg L(-1), the alteration of valve rhythm behavior was correlated with Cu concentration within 30 min, indicating notable sensing ability. This study outlines the feasibility of an in situ early warning dynamic biomonitoring system for detection of waterborne Cu based on circadian valve...
ABSTRACT The purpose of this study was to develop an improved clam-based online behavioral monito... more ABSTRACT The purpose of this study was to develop an improved clam-based online behavioral monitoring system. The improved system consists of a valvometry apparatus and three kinds of valve closure analytic programs that can offer a real-time and cost-effective valvometric technique to effectively detect valve activities of clams Corbicula fluminea. The improved valvometry apparatus mainly applied electromagnetic mutual-inducting actions and principles to measure the distance between two inductances as the sensing theory of determining the magnitude of the bivalve shell gape. It evolved into a noninvasive and unconstrained valvometric technique, allowing freshwater bivalves to move freely and burrow in the bottom sands of observed tanks with minimal experimental constraint. By employing a compiled LabVIEW graphic control program on a personal computer, the clam-based online behavioral monitoring system can monitor and analyze the daily valve activities of a group of clams in real time. A strict laboratory procedure associated with an improved valvometric technique was performed in the bioassay experiment to observe the magnitudes of shell gape as the determining thresholds of valve closing (VC) and siphon extension (SE) statuses. A statistics-based approach describing bivalve behavioral movements of C. fluminea exposed to unpolluted environment was performed. Our results demonstrated that the magnitudes of shell gape of 20% and 50% can be adopted as the determining thresholds of VC and SE statuses, respectively, to digitalize the valve movements in bivalves, allowing a well-established sequence framework for quantifying the characterization of the daily valve behavioral rhythm.
Environmental science and pollution research international, Jan 13, 2015
This study successfully applied an improved valvometry technique to measure waterborne copper (Cu... more This study successfully applied an improved valvometry technique to measure waterborne copper (Cu), based on valve activity dynamics of the freshwater clam Corbicula fluminea. The improved valvometry technique allows the use of free-range bivalves and avoids causing stresses from experimental artifacts. The proposed daily valve rhythm models and a toxicodynamics-based Hill model were linked to predict valve dynamic responses under different Cu exposures with a circadian valve rhythm endpoint. Cu-specific detection threshold was 5.6 (95 % CI 2.1-9.3) and 19.5 (14.6-24.3) μg L(-1) for C. fluminea, based on response times of 300 and 30 min, respectively. Upon exposure to Cu concentrations in excess of 50 μg L(-1), the alteration of valve rhythm behavior was correlated with Cu concentration within 30 min, indicating notable sensing ability. This study outlines the feasibility of an in situ early warning dynamic biomonitoring system for detection of waterborne Cu based on circadian valve...
ABSTRACT The purpose of this study was to develop an improved clam-based online behavioral monito... more ABSTRACT The purpose of this study was to develop an improved clam-based online behavioral monitoring system. The improved system consists of a valvometry apparatus and three kinds of valve closure analytic programs that can offer a real-time and cost-effective valvometric technique to effectively detect valve activities of clams Corbicula fluminea. The improved valvometry apparatus mainly applied electromagnetic mutual-inducting actions and principles to measure the distance between two inductances as the sensing theory of determining the magnitude of the bivalve shell gape. It evolved into a noninvasive and unconstrained valvometric technique, allowing freshwater bivalves to move freely and burrow in the bottom sands of observed tanks with minimal experimental constraint. By employing a compiled LabVIEW graphic control program on a personal computer, the clam-based online behavioral monitoring system can monitor and analyze the daily valve activities of a group of clams in real time. A strict laboratory procedure associated with an improved valvometric technique was performed in the bioassay experiment to observe the magnitudes of shell gape as the determining thresholds of valve closing (VC) and siphon extension (SE) statuses. A statistics-based approach describing bivalve behavioral movements of C. fluminea exposed to unpolluted environment was performed. Our results demonstrated that the magnitudes of shell gape of 20% and 50% can be adopted as the determining thresholds of VC and SE statuses, respectively, to digitalize the valve movements in bivalves, allowing a well-established sequence framework for quantifying the characterization of the daily valve behavioral rhythm.
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