KAILIANG CHEN*, JIA LIAO†, XIAOYU SHANG‡, AND JOHNNY SIU-HANG LI§ It is our pleasure to congratul... more KAILIANG CHEN*, JIA LIAO†, XIAOYU SHANG‡, AND JOHNNY SIU-HANG LI§ It is our pleasure to congratulate Professor Andrew Cairns and his coauthors on this timely paper, which provides valuable information to users of stochastic mortality models. The findings from this paper indicate that the CBD class of models performs well, particularly when the robustness of parameter estimates is taken into account. The objective of this discussion is to expand the CBD class of models by considering two transformations, log and probit, in addition to the logit transformation that is used in the original specification. The new variants are compared against the original CBD model by some quantitative means.
MotivationOscillatory genes, with periodic expression at the mRNA and/or protein level, have been... more MotivationOscillatory genes, with periodic expression at the mRNA and/or protein level, have been shown to play a pivotal role in many biological contexts. However, with the exception of the circadian clock and cell cycle, only a few such genes are known. Detecting oscillatory genes from snapshot single-cell experiments is a challenging task due to the lack of time information. Oscope is a recently proposed method to identify co-oscillatory gene pairs using single-cell RNA-seq data. Although promising, the current implementation of Oscope does not provide a principled statistical criterion for selecting oscillatory genes.ResultsWe improve the optimisation scheme underlying Oscope and provide a well-calibrated non-parametric hypothesis test to select oscillatory genes at a given FDR threshold. We evaluate performance on synthetic data and three real datasets and show that our approach is more sensitive than the original Oscope formulation, discovering larger sets of known oscillators...
... Japonicus sequences up to 1000 bp upstream. Download: http://www.cl.cam.ac.uk/~ pl219/biocomp... more ... Japonicus sequences up to 1000 bp upstream. Download: http://www.cl.cam.ac.uk/~ pl219/biocomp_2007.pdf CACHED: Download as a PDF. by Claudia Angelini , Italia De Feis , Luisa Cutillo , Pietro Liò , Richard Van Der Wath. ...
Cloud detection from geostationary satellite multispectral images through statistical discriminan... more Cloud detection from geostationary satellite multispectral images through statistical discriminant analysis is investigated. Methods are tested on MSG-SEVIRI multispectral data for which MODIS cloud mask was used to train classification.
A kindred with hereditary spherocytosis and beta-thalassaemia trait was identified. Detailed stud... more A kindred with hereditary spherocytosis and beta-thalassaemia trait was identified. Detailed studies of the red cell membrane proteins on polyacrylamide gels with sodium dodecyl sulphate (SDS-PAGE) demonstrated the presence of band 3 (anion transporter) deficiency in all HS subjects (20-25% reduction) whereas spectrin content was in the normal range. The molecular defect of beta thalassaemia in this kindred was due to a beta(0) codon 39 (C-T) mutation, as assessed by beta globin gene amplification and ASO-probe hybridization. Seven subjects of this family were studied: two were normal, two had HS alone, two co-inherited HS and beta-thalassaemia trait, and one had beta-thalassaemia trait only. The two subjects with HS alone had a typical clinical form of spherocytosis with anaemia, reticulocytosis and increased red cell osmotic fragility. The two with both HS and beta-thalassaemia trait were not anaemic and showed a small, well-compensated haemolysis. Hence the finding of red cells with abnormalities of both HS and beta-thalassaemia indicates that beta-thalassaemic trait 'silences' HS caused by band 3 deficiency.
KAILIANG CHEN*, JIA LIAO†, XIAOYU SHANG‡, AND JOHNNY SIU-HANG LI§ It is our pleasure to congratul... more KAILIANG CHEN*, JIA LIAO†, XIAOYU SHANG‡, AND JOHNNY SIU-HANG LI§ It is our pleasure to congratulate Professor Andrew Cairns and his coauthors on this timely paper, which provides valuable information to users of stochastic mortality models. The findings from this paper indicate that the CBD class of models performs well, particularly when the robustness of parameter estimates is taken into account. The objective of this discussion is to expand the CBD class of models by considering two transformations, log and probit, in addition to the logit transformation that is used in the original specification. The new variants are compared against the original CBD model by some quantitative means.
MotivationOscillatory genes, with periodic expression at the mRNA and/or protein level, have been... more MotivationOscillatory genes, with periodic expression at the mRNA and/or protein level, have been shown to play a pivotal role in many biological contexts. However, with the exception of the circadian clock and cell cycle, only a few such genes are known. Detecting oscillatory genes from snapshot single-cell experiments is a challenging task due to the lack of time information. Oscope is a recently proposed method to identify co-oscillatory gene pairs using single-cell RNA-seq data. Although promising, the current implementation of Oscope does not provide a principled statistical criterion for selecting oscillatory genes.ResultsWe improve the optimisation scheme underlying Oscope and provide a well-calibrated non-parametric hypothesis test to select oscillatory genes at a given FDR threshold. We evaluate performance on synthetic data and three real datasets and show that our approach is more sensitive than the original Oscope formulation, discovering larger sets of known oscillators...
... Japonicus sequences up to 1000 bp upstream. Download: http://www.cl.cam.ac.uk/~ pl219/biocomp... more ... Japonicus sequences up to 1000 bp upstream. Download: http://www.cl.cam.ac.uk/~ pl219/biocomp_2007.pdf CACHED: Download as a PDF. by Claudia Angelini , Italia De Feis , Luisa Cutillo , Pietro Liò , Richard Van Der Wath. ...
Cloud detection from geostationary satellite multispectral images through statistical discriminan... more Cloud detection from geostationary satellite multispectral images through statistical discriminant analysis is investigated. Methods are tested on MSG-SEVIRI multispectral data for which MODIS cloud mask was used to train classification.
A kindred with hereditary spherocytosis and beta-thalassaemia trait was identified. Detailed stud... more A kindred with hereditary spherocytosis and beta-thalassaemia trait was identified. Detailed studies of the red cell membrane proteins on polyacrylamide gels with sodium dodecyl sulphate (SDS-PAGE) demonstrated the presence of band 3 (anion transporter) deficiency in all HS subjects (20-25% reduction) whereas spectrin content was in the normal range. The molecular defect of beta thalassaemia in this kindred was due to a beta(0) codon 39 (C-T) mutation, as assessed by beta globin gene amplification and ASO-probe hybridization. Seven subjects of this family were studied: two were normal, two had HS alone, two co-inherited HS and beta-thalassaemia trait, and one had beta-thalassaemia trait only. The two subjects with HS alone had a typical clinical form of spherocytosis with anaemia, reticulocytosis and increased red cell osmotic fragility. The two with both HS and beta-thalassaemia trait were not anaemic and showed a small, well-compensated haemolysis. Hence the finding of red cells with abnormalities of both HS and beta-thalassaemia indicates that beta-thalassaemic trait 'silences' HS caused by band 3 deficiency.
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