following treatment with diminazene aceturate. congolense arising in infected animals trypanosomes in populations of Trypanosoma Frequency of diminazene-resistant
Aim: The study was carried out to determine the in vivo antitrypanosomal effect of methanolic extract of Zingiber officinale (ginger) in Trypanosoma brucei brucei-infected mice. Materials and Methods: Twenty-five mice were randomly... more
Aim: The study was carried out to determine the in vivo antitrypanosomal effect of methanolic extract of Zingiber officinale (ginger) in Trypanosoma brucei brucei-infected mice. Materials and Methods: Twenty-five mice were randomly allocated into five groups of five animals each. Group I and II were given Tween 80 (1 ml/kg) and diminazene aceturate (3.5 mg/kg) to serve as untreated and treated controls, respectively. Groups III-V received the extract at 200, 400 and 800 mg/kg body weight, respectively. All treatments were given for 6 consecutive days and through the oral route. The mean body weight, mean survival period and daily level of parasitaemia were evaluated. Results: Acute toxicity showed the extract to be relatively safe. There was an insignificant increase in body weight and survival rate of mice treated with the extract. The level of parasitaemia in the extract treated groups was decreased. Conclusion: This study shows the in vivo potential of methanolic extract of Z. of...
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The need to have a screening tool for onchocerciasis control to compliment parasitological and molecular diagnosis has remained an important goal. A novel Onchocerca volvulus slide flocculation test (Ov-SFT) was developed using tissue... more
The need to have a screening tool for onchocerciasis control to compliment parasitological and molecular diagnosis has remained an important goal. A novel Onchocerca volvulus slide flocculation test (Ov-SFT) was developed using tissue homogenates from rat organs (kidney, liver, heart, spleen, muscle, brain, lungs and testes). Liver followed by spleen and then kidney tissue homogenates performed better than others. Flocculation reactions were performed with antigens extracted from female adult worms of O. volvulus using sodium dodecyl sulphate (SDS) and sera from confirmed onchocerciasis infected patients. Negative and assay controls included non-infected serum and phosphate buffered saline (PBS). Results were subjectively graded as no reaction, 0 or negative. Positive reactions were rated 1 to 5 for low, moderate, medium, high and very high flocculation. The grading corresponded to titre levels ranging from 1/2, 1/4, 1/8, 1/16 and 1/32. The liver homogenate had the highest titre of ...
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Loxostylis alata belongs to the family Anacardiaceae. In South Africa, the bark and leaves of Loxostylis alata are used in traditional medicine during childbirth and also to stimulate the immune system. This study was aimed at... more
Loxostylis alata belongs to the family Anacardiaceae. In South Africa, the bark and leaves of Loxostylis alata are used in traditional medicine during childbirth and also to stimulate the immune system. This study was aimed at investigating the antiinflammatory and analgesic activities of the crude acetone extract of Loxostylis alata. In vivo experimental models that include egg albumin, prostaglandin E2, and histamine-induced-paw oedema, and vascular permeability test were used to screen the extract for antiinflammatory effect. While formalin-induced nociception, acetic acidinduced writhing and hot plate tests were carried out to evaluate analgesic effects of the extract. The extract inhibited egg-albumin, prostaglandin and histamine-induced oedema in a dose-dependent (50-200 mg/kg) manner. It also inhibited vascular permeability cause by acetic acid in a similar fashion. Furthermore, the extract inhibited pain induced by formalin; acetic acid and heat dosedependently (50-200 mg/kg...
Ongoing treatment control strategy against onchocerciasis or river blindness will need efficient diagnostic method to evaluate the mass drug administration with ivermectin (Mectizan®). Sole reliance on classical parasitological method of... more
Ongoing treatment control strategy against onchocerciasis or river blindness will need efficient diagnostic method to evaluate the mass drug administration with ivermectin (Mectizan®). Sole reliance on classical parasitological method of skin snip microscopy for detecting microfilaria has proved less sensitive during post-control period. Detecting any parts of the parasite stages such as antigens, enzymes and nucleic acids (DNA and RNA) is a definitive diagnosis and highly sensitive. This study was to evaluate the diagnostic reliability of the beta actin gene primer pair to confirm its suitability for validating presence or absence of skin microfilaria at post-treatment. DNA extracted from skin snip samples (n=15) from an onchocerciasis mesoendemic area, three from non-endemic, two adult worm fragments and blank wells with only master mix (n=7) were subjected to endpoint polymerase chain reaction (PCR) analysis. Four of the samples had shown reactivity with first internal transcribe...
This study evaluated in vitro and in vivo antitrypanosomal effect of artesunate and/or diminazene aceturate in Wistar rats experimentally infected with Trypanosoma brucei brucei. In vitro screening was carried out in triplicates using 50... more
This study evaluated in vitro and in vivo antitrypanosomal effect of artesunate and/or diminazene aceturate in Wistar rats experimentally infected with Trypanosoma brucei brucei. In vitro screening was carried out in triplicates using 50 μl of 0.2, 2 and 20 μg/μl of artesunate as test drug; diminazene aceturate, normal saline and trypanosome-infected blood served as controls in a 96-well microtitre plate, incubated at 37˚C for 5 minutes. Efficacy was observed over a period of 60 minutes for reduced or complete trypanosomal immobilization. Results showed concentration-dependent cessation of trypanosomal motility was significantly (p < 0.001) induced by artesunate when compared to the controls. Seventy Wistar rats of both sexes weighing between 190 and 210 g were randomly divided into 7 groups (5 males and 5 females) are used for in vivo study. Groups I and II served as normal control and model control respectively. Groups III to VII were infected with Trypanosoma brucei brucei (1...
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Research Interests: Biology, Nigeria, Medicine, Mice, Animals, and 3 moreFreeze Drying, Species Specificity, and Agaricales
Research Interests: Microbiology, Biology, Medicine, Veterinary, Veterinary Parasitology, and 15 moreSpermatogenesis, Animals, Male, Fisheries Sciences, Sterilization, tESTIS, Experimental Infection, Cattle, Time Factors, Degeneration, Veterinary Sciences, Epididymis, Control Group, Trypanosoma vivax, and cattle diseases
... Infective larvae (L3) of the hookworm were obtained from the Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria. ... 436 p. 7. Chiezey, NP, AG Jagun, PA Abdu, CBI Alawa, SO... more
... Infective larvae (L3) of the hookworm were obtained from the Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria. ... 436 p. 7. Chiezey, NP, AG Jagun, PA Abdu, CBI Alawa, SO Magaji, JO Gefu, and LO Eduvie. 2000. ...