Wild Crop Relatives: Genomic and Breeding Resources, 2011
Page 1. Chapter 7 Santalum Madhugiri Nageswara Rao, Jaya R. Soneji, and Padmini Sudarshana 7.1 Ba... more Page 1. Chapter 7 Santalum Madhugiri Nageswara Rao, Jaya R. Soneji, and Padmini Sudarshana 7.1 Basic Botany of the Species The genus Santalum, commonly known as sandal-wood, belongs to the family Santalaceae (Fig. 7.1). ...
Banana is an economically important fruit as well as food security crop. Though it originated in ... more Banana is an economically important fruit as well as food security crop. Though it originated in the Southeast Asian region, it is widely grown around the world. Conventionally, bananas are vegetatively propagated by suckers or corms, while the seeds are employed for propagation only in breeding programs. Vegetative propagation is the major cause of introduction and spread of diseases to non-infected areas. To overcome this problem, tissue-cultured banana plants are being used as planting material. Somaclonal variation (SV) appears to be widespread among banana plants regenerated by tissue culture. A number of factors are known to induce SV which can be problematic during banana micropropagation, although, it may be utilized as an efficient tool in plant breeding. Somaclones have been identified based on their morphological or agronomic characteristics. Early detection of SV is, therefore, very useful. A morphological, biochemical, cytogenetic and/or molecular biology based approach...
Page 1. Citrus Breeding Fred G. Gmitter Jr., Jaya R. Soneji, and Madhugiri Nageswara Rao 1 Introd... more Page 1. Citrus Breeding Fred G. Gmitter Jr., Jaya R. Soneji, and Madhugiri Nageswara Rao 1 Introduction With a total world production of 105.4 million tons, citrus is one of the world's most important fruit crops (FAO 2006). Its ...
Eight new green fluorescent protein (GFP) binary vectors were developed by inserting gfp reporter... more Eight new green fluorescent protein (GFP) binary vectors were developed by inserting gfp reporter gene cassettes into pGreen vectors. We chose one of them, pG52KF, with the nptII selection and gfp reporter gene and one recombinant construct, pG52KFp, for a preliminary evaluation in citrus using Agrobacterium-mediated transformation. High-transformation efficiency was observed, whereas green fluorescence greatly facilitated the early in vivo screening and categorizing of the transformants. These pGreen-derived GFP binary vectors, freely available on request, provide more and flexible options for genetic transformation in citrus and other woody plants.
Journal of the American Society for Horticultural Science
Seeds of Coreopsis leavenworthii Torr. & Gray (Asteraceae) are being commercially produced but th... more Seeds of Coreopsis leavenworthii Torr. & Gray (Asteraceae) are being commercially produced but the lack of genetic diversity information has hindered growers and end users from addressing several critical issues affecting wild collection, commercial production, distribution, and the use of seeds. In this study, the genetic diversity and differentiation among natural, production, and introduced populations were analyzed at the molecular level using 320 amplified fragment length polymorphism (AFLP) markers. A high level of diversity [68.6% average polymorphism; total genetic diversity (H t ) = 0.309] and a moderate level of genetic differentiation [total genetic diversity residing among populations (G st ) = 0.226; Φ st = 0.244; Bayesian analog of Nei's G st (G st -B) = 0.197] was detected among six natural populations—two each from northern, central, and southern Florida. Two distance-based clustering analyses, based on an individual's AFLP phenotypes or a population's al...
Wild Crop Relatives: Genomic and Breeding Resources, 2011
Poncirus, a close relative of citrus, is a valuable genetic resource for the genetic improvement ... more Poncirus, a close relative of citrus, is a valuable genetic resource for the genetic improvement of citrus. It is resistant to many diseases and pests of citrus and is cold hardy. However, it has not been domesticated or commercialized on a large scale as it has inedible fruits. It is widely used as a rootstock for citrus and has largely
Wild Crop Relatives: Genomic and Breeding Resources, 2011
ABSTRACT Citrus is one of the most important fruit crops grown in the subtropical and tropical ar... more ABSTRACT Citrus is one of the most important fruit crops grown in the subtropical and tropical areas of the world and has great economic, health, religious, and cultural values. While there are conservation and management efforts, the lack of sufficient descriptions, specimens, and original habitats has made it difficult for the biogeographers to define precisely the centers of origin and ancestors of citrus. Understanding taxonomy, phylogenetic relationships, and genetic variability in citrus is critical for determining its origin, evolution, and efficient genetic improvement programs. Though citrus breeding is very challenging, different breeding programs throughout the world have made significant progress in the application of conventional and modern approaches for genetic improvement and cultivar development. The recent knowledge and establishment of an array of genomics and bioinformatics tools have provided efficient strategies to identify, tag, and clone many economically important genes. Progress toward these efforts in citrus has been discussed.
Wild Crop Relatives: Genomic and Breeding Resources, 2011
Page 1. Chapter 7 Santalum Madhugiri Nageswara Rao, Jaya R. Soneji, and Padmini Sudarshana 7.1 Ba... more Page 1. Chapter 7 Santalum Madhugiri Nageswara Rao, Jaya R. Soneji, and Padmini Sudarshana 7.1 Basic Botany of the Species The genus Santalum, commonly known as sandal-wood, belongs to the family Santalaceae (Fig. 7.1). ...
Banana is an economically important fruit as well as food security crop. Though it originated in ... more Banana is an economically important fruit as well as food security crop. Though it originated in the Southeast Asian region, it is widely grown around the world. Conventionally, bananas are vegetatively propagated by suckers or corms, while the seeds are employed for propagation only in breeding programs. Vegetative propagation is the major cause of introduction and spread of diseases to non-infected areas. To overcome this problem, tissue-cultured banana plants are being used as planting material. Somaclonal variation (SV) appears to be widespread among banana plants regenerated by tissue culture. A number of factors are known to induce SV which can be problematic during banana micropropagation, although, it may be utilized as an efficient tool in plant breeding. Somaclones have been identified based on their morphological or agronomic characteristics. Early detection of SV is, therefore, very useful. A morphological, biochemical, cytogenetic and/or molecular biology based approach...
Page 1. Citrus Breeding Fred G. Gmitter Jr., Jaya R. Soneji, and Madhugiri Nageswara Rao 1 Introd... more Page 1. Citrus Breeding Fred G. Gmitter Jr., Jaya R. Soneji, and Madhugiri Nageswara Rao 1 Introduction With a total world production of 105.4 million tons, citrus is one of the world's most important fruit crops (FAO 2006). Its ...
Eight new green fluorescent protein (GFP) binary vectors were developed by inserting gfp reporter... more Eight new green fluorescent protein (GFP) binary vectors were developed by inserting gfp reporter gene cassettes into pGreen vectors. We chose one of them, pG52KF, with the nptII selection and gfp reporter gene and one recombinant construct, pG52KFp, for a preliminary evaluation in citrus using Agrobacterium-mediated transformation. High-transformation efficiency was observed, whereas green fluorescence greatly facilitated the early in vivo screening and categorizing of the transformants. These pGreen-derived GFP binary vectors, freely available on request, provide more and flexible options for genetic transformation in citrus and other woody plants.
Journal of the American Society for Horticultural Science
Seeds of Coreopsis leavenworthii Torr. & Gray (Asteraceae) are being commercially produced but th... more Seeds of Coreopsis leavenworthii Torr. & Gray (Asteraceae) are being commercially produced but the lack of genetic diversity information has hindered growers and end users from addressing several critical issues affecting wild collection, commercial production, distribution, and the use of seeds. In this study, the genetic diversity and differentiation among natural, production, and introduced populations were analyzed at the molecular level using 320 amplified fragment length polymorphism (AFLP) markers. A high level of diversity [68.6% average polymorphism; total genetic diversity (H t ) = 0.309] and a moderate level of genetic differentiation [total genetic diversity residing among populations (G st ) = 0.226; Φ st = 0.244; Bayesian analog of Nei's G st (G st -B) = 0.197] was detected among six natural populations—two each from northern, central, and southern Florida. Two distance-based clustering analyses, based on an individual's AFLP phenotypes or a population's al...
Wild Crop Relatives: Genomic and Breeding Resources, 2011
Poncirus, a close relative of citrus, is a valuable genetic resource for the genetic improvement ... more Poncirus, a close relative of citrus, is a valuable genetic resource for the genetic improvement of citrus. It is resistant to many diseases and pests of citrus and is cold hardy. However, it has not been domesticated or commercialized on a large scale as it has inedible fruits. It is widely used as a rootstock for citrus and has largely
Wild Crop Relatives: Genomic and Breeding Resources, 2011
ABSTRACT Citrus is one of the most important fruit crops grown in the subtropical and tropical ar... more ABSTRACT Citrus is one of the most important fruit crops grown in the subtropical and tropical areas of the world and has great economic, health, religious, and cultural values. While there are conservation and management efforts, the lack of sufficient descriptions, specimens, and original habitats has made it difficult for the biogeographers to define precisely the centers of origin and ancestors of citrus. Understanding taxonomy, phylogenetic relationships, and genetic variability in citrus is critical for determining its origin, evolution, and efficient genetic improvement programs. Though citrus breeding is very challenging, different breeding programs throughout the world have made significant progress in the application of conventional and modern approaches for genetic improvement and cultivar development. The recent knowledge and establishment of an array of genomics and bioinformatics tools have provided efficient strategies to identify, tag, and clone many economically important genes. Progress toward these efforts in citrus has been discussed.
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