An 83-year-old male patient is admitted to the central hospital in Cumana, Venezuela with severe ... more An 83-year-old male patient is admitted to the central hospital in Cumana, Venezuela with severe urinary infection, history of hospitalizaions and prolonged antimicrobial treatments. A strain of Enterobacter cloacae was isolated showing resistance to multiple types of antibiotics (only sensitive to gentamicin), with phenotype of serine- and metallo-carbapenemases. Both, bla(VIM-2) and bla(KPC) genes were detected in the isolate. This is the first report of an Enterobacteriaceae species producing both KPC carbapenemase and VIM metallo carbapenemase in Venezuela. This finding has a great clinical and epidemiological impact in the region, because of the feasibility of transferring these genes, through mobile elements to other strains of Enterobacter and to other infection-causing species of bacteria.
In recent years there has been a worldwide raise in the isolation of Pseudomonas aeruginosa strai... more In recent years there has been a worldwide raise in the isolation of Pseudomonas aeruginosa strains with resistance to multiple antibiotics, being β-lactamase production the main cause of resistance to β-lactamic antibiotic (which are the only therapeutic option in many cases). The MBLs are an β-lactamic- degrading enzymatic family that have emerged as clinically relevant resistance determinants and are active against carbapemens, don´t hydrolise monobactams, have one or two zinc ions in its active site and are inhibited by metallic ions chelating agents such as EDTA and dipicolinic acid, but aren´t inhibited by β-lactamase inhibitors such as clavulanic acid, sulbactam or tazobactam. This report describes the detection of VIM-type MBLs by phenotypic and molecular methods in 9 carbapenemsresistant Pseudomonas aeruginosa strains isolated from clinical samples of four Venezuelan hospitals. By phenotypic methods it was evidenced that 100% of the strains were MBLs producers, and by final...
Revista panamericana de salud pública = Pan American journal of public health, 2011
To determine the frequency of enzymatic mechanisms associated with reduced sensitivity to broad-s... more To determine the frequency of enzymatic mechanisms associated with reduced sensitivity to broad-spectrum beta-lactam antibiotics in enterobacteria isolates obtained at hospital centers in Caracas, Venezuela. A cross-sectional study was conducted on enterobacteria isolated from patients at eight hospital centers in Caracas, Venezuela, from 15 October 2009 to 15 January 2010. The species were identified using conventional biochemical tests, and their susceptibility to antimicrobial drugs was assessed by antibiogram (Kirby-Bauer method), using the 2010 performance standards published by the Clinical and Laboratory Standards Institute. Beta-lactam-resistant genes were detected using an enhanced polymerase chain reaction assay. Of 1 235 isolates, 207 (16.8%) exhibited resistance to third- and fourth-generation cephalosporins, carbapenems, or both. They presented the following phenotypes: extended-spectrum beta-lactamase (ESBL), 93.8%; depressed AmpC, 4.3%; and carbapenemase, 1.9%. Furthe...
An 83-year-old male patient is admitted to the central hospital in Cumana, Venezuela with severe ... more An 83-year-old male patient is admitted to the central hospital in Cumana, Venezuela with severe urinary infection, history of hospitalizaions and prolonged antimicrobial treatments. A strain of Enterobacter cloacae was isolated showing resistance to multiple types of antibiotics (only sensitive to gentamicin), with phenotype of serine- and metallo-carbapenemases. Both, bla(VIM-2) and bla(KPC) genes were detected in the isolate. This is the first report of an Enterobacteriaceae species producing both KPC carbapenemase and VIM metallo carbapenemase in Venezuela. This finding has a great clinical and epidemiological impact in the region, because of the feasibility of transferring these genes, through mobile elements to other strains of Enterobacter and to other infection-causing species of bacteria.
In recent years there has been a worldwide raise in the isolation of Pseudomonas aeruginosa strai... more In recent years there has been a worldwide raise in the isolation of Pseudomonas aeruginosa strains with resistance to multiple antibiotics, being β-lactamase production the main cause of resistance to β-lactamic antibiotic (which are the only therapeutic option in many cases). The MBLs are an β-lactamic- degrading enzymatic family that have emerged as clinically relevant resistance determinants and are active against carbapemens, don´t hydrolise monobactams, have one or two zinc ions in its active site and are inhibited by metallic ions chelating agents such as EDTA and dipicolinic acid, but aren´t inhibited by β-lactamase inhibitors such as clavulanic acid, sulbactam or tazobactam. This report describes the detection of VIM-type MBLs by phenotypic and molecular methods in 9 carbapenemsresistant Pseudomonas aeruginosa strains isolated from clinical samples of four Venezuelan hospitals. By phenotypic methods it was evidenced that 100% of the strains were MBLs producers, and by final...
Revista panamericana de salud pública = Pan American journal of public health, 2011
To determine the frequency of enzymatic mechanisms associated with reduced sensitivity to broad-s... more To determine the frequency of enzymatic mechanisms associated with reduced sensitivity to broad-spectrum beta-lactam antibiotics in enterobacteria isolates obtained at hospital centers in Caracas, Venezuela. A cross-sectional study was conducted on enterobacteria isolated from patients at eight hospital centers in Caracas, Venezuela, from 15 October 2009 to 15 January 2010. The species were identified using conventional biochemical tests, and their susceptibility to antimicrobial drugs was assessed by antibiogram (Kirby-Bauer method), using the 2010 performance standards published by the Clinical and Laboratory Standards Institute. Beta-lactam-resistant genes were detected using an enhanced polymerase chain reaction assay. Of 1 235 isolates, 207 (16.8%) exhibited resistance to third- and fourth-generation cephalosporins, carbapenems, or both. They presented the following phenotypes: extended-spectrum beta-lactamase (ESBL), 93.8%; depressed AmpC, 4.3%; and carbapenemase, 1.9%. Furthe...
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Papers by Daniel Marcano