Sinovac is an inactivated virus COVID 19 vaccine given emergency authorization for COVID 19 Pande... more Sinovac is an inactivated virus COVID 19 vaccine given emergency authorization for COVID 19 Pandemic. Different adverse reactions have been seen in after marketing of COVID 19 vaccines. Here we present a case of patient who developed cerebral venous sinus thrombosis two weeks after the first dose of SINOVAC vaccine.
The sorption mechanism of hexavalent chromium sorption on eucalyptus barks was evaluated as a fun... more The sorption mechanism of hexavalent chromium sorption on eucalyptus barks was evaluated as a function of solution pH for different adsorbent dosages, surface coverage, and the amount of adsorbent. The chromium retention was evaluated based on the distribution coefficient (D), and this retention is attributed to species, which is predominant between pH 1 and 6.5. The biosorption of Cr(VI) ions onto barks achieved at pH 2.0 in the highest sorbet conditions corresponding to [Cr(VI)] = 10–5 mol (V = 100 mL) is examined for various surface coverage. The surface complexes formed between chromate and eucalyptus barks were found to be > S (HCrO4) and > S (CrO4). Logarithmic stability for log K1–1 and the log K10 values of the complexes were measured and found to be -5.93 in acidic medium and -0.76 in alkaline medium, respectively. Pointed out that the adsorption of Cr(VI) on eucalyptus bark was greater than 90% in all cases, Cr(VI) recovery is strongly acidic dependent and shows max...
Un montón de detalles se encienden y se apagan, sin embargo, en mi memoria visual. Este pasaje re... more Un montón de detalles se encienden y se apagan, sin embargo, en mi memoria visual. Este pasaje revela la presencia de lo cinematográfico en la narrativa bryceana. Tanto la historia de Manongo como la aproximación misma del narrador, manifiestan una formación dentro de una cultura marcada por el cine, sus héroes, sus historias y, sobre todo, sus imágenes. La historia de amor
Vad kännetecknar SUHF-modellen respektive ABC-systemet? Hur är SUHF-modellen uppbyggd jämfört med... more Vad kännetecknar SUHF-modellen respektive ABC-systemet? Hur är SUHF-modellen uppbyggd jämfört med ABC-systemet? Syfte: Syftet med den här uppsatsen är att beskriva och analysera de skillnader och likheter som finns mellan SUHF-modellen och ABCsystemet. Metod: Studien var av kvalitativ karaktär och baserades på sekundärdata i form av vetenskapliga artiklar och böcker inom ämnet ABC-systemet i lärosäten. Som empiriskt underlag gjordes en semistrukturerad intervju med ekonomichefen på Mälardalens högskola för att få information om SUHF-modellen. Även rapporter framtagna av SUHF:s arbetsgrupp användes som underlag till den empiriska delen. Slutsats: Målet med de båda modellerna är att full kostnadstäckning ska ske genom att fördela de direkta och indirekta kostnaderna på ett tillfredställande vis till kostnadsobjektet/kostnadsbäraren. En tydlig skillnad som vi kan se mellan modellerna är att ABC-systemets fokus ligger på aktivitetsgrupper och aktiviteter medan SUHF-modellens fokus ligger på kärnverksamheten respektive stödverksamheten men att de i grund och botten är benämningar för samma utföranden. Vi anser slutligen att SUHF-modellen är ett tillräckligt effektivt kostnadssystem som är väl anpassat efter svenska lärosätens behov och som bör fortsätta att användas och utvecklas.
ABSTRACTAdenoviral infections in the immunocompromised host are associated with significant morbi... more ABSTRACTAdenoviral infections in the immunocompromised host are associated with significant morbidity and mortality. Although the adoptive transfer of adenovirus-specific T cells may prevent and treat such infections, the T-cell immune response to the multiplicity of adenovirus serotypes and subspecies that infect humans has not been well characterized, impeding the development of such approaches. We have, therefore, analyzed the specificities of T-cell responses to the viral capsid hexon antigen, since this structure is highly conserved in human pathogens. We screened 25 human cytotoxic T-cell lines with adenovirus specificity to extensively characterize their responses to adenoviral hexon and to identify a panel of novel CD4+and CD8+T-cell epitopes. Using a peptide library spanning the entire sequence of the hexon protein, we confirmed the responsiveness of these cytotoxic T-cell lines to seven peptides described previously and also identified 33 new CD4- or CD8-restricted hexon e...
Background: Gas Permeable Rapid Expansion (G-Rex) bioreactors have been shown to efficiently expa... more Background: Gas Permeable Rapid Expansion (G-Rex) bioreactors have been shown to efficiently expand immune cells intended for therapeutic use, but do not address the complexity of the viral transduction step required for many engineered T-cell products. Here we demonstrate a novel method for transduction of activated T cells with Vectofusin-1 reagent. Transduction is accomplished in suspension, in G-Rex bioreactors. The simplified transduction step is integrated into a streamlined process that uses a single bioreactor with limited operator intervention. Methods: Peripheral blood mononuclear cells (PBMCs) from healthy donors were thawed, washed and activated with soluble anti-CD3 and anti-CD28 antibodies either in cell culture bags or in G-Rex bioreactors. Cells were cultured in TexMACS GMP medium with interleukin (IL)-7 and IL-15 and transduced with RetroNectin in bags or Vectorfusin-1 in the G-Rex. Total viable cell number, fold expansion, viability, transduction efficiency, phenotype and function were compared between the two processes. Results: The simplified process uses a single vessel from activation through harvest and achieves 56% transduction with 29-fold expansion in 11 days. The cells generated in the simplified process do not differ from cells produced in the conventional bag-based process functionally or phenotypically. Discussion: This study demonstrates that T cells can be transduced in suspension. Further, the conventional method of generating engineered T cells in bags for clinical use can be streamlined to a much simpler, lessexpensive process without compromising the quality or function of the cell product.
Introduction: Genetically modified T cells are being investigated to treat a variety of disorders... more Introduction: Genetically modified T cells are being investigated to treat a variety of disorders and have been particularly successful in treating B cell cancers. As more effort is poured into new targets, molecular switches, and various other modifications, development of processes to quickly manufacture new products must keep up. Current manufacturing processes often require highly skilled operators and specialized equipment. Here, we demonstrate a simplified, novel method for transduction of T cells, followed by robust expansion in the G-Rex bioreactor with no need for intervention until harvest. A scaled-up, closed-version of the same process, including a closed harvest step with the GatheRex is currently under evaluation. Methods: Frozen peripheral blood mononuclear cells (PBMCs) from healthy donors were used as starting material. PBMCs were thawed, washed, and activated with soluble anti-CD3 and anti-CD28 antibodies either in cell culture bags (32-C, Saint-Gobain Cell Therapy...
Sinovac is an inactivated virus COVID 19 vaccine given emergency authorization for COVID 19 Pande... more Sinovac is an inactivated virus COVID 19 vaccine given emergency authorization for COVID 19 Pandemic. Different adverse reactions have been seen in after marketing of COVID 19 vaccines. Here we present a case of patient who developed cerebral venous sinus thrombosis two weeks after the first dose of SINOVAC vaccine.
The sorption mechanism of hexavalent chromium sorption on eucalyptus barks was evaluated as a fun... more The sorption mechanism of hexavalent chromium sorption on eucalyptus barks was evaluated as a function of solution pH for different adsorbent dosages, surface coverage, and the amount of adsorbent. The chromium retention was evaluated based on the distribution coefficient (D), and this retention is attributed to species, which is predominant between pH 1 and 6.5. The biosorption of Cr(VI) ions onto barks achieved at pH 2.0 in the highest sorbet conditions corresponding to [Cr(VI)] = 10–5 mol (V = 100 mL) is examined for various surface coverage. The surface complexes formed between chromate and eucalyptus barks were found to be > S (HCrO4) and > S (CrO4). Logarithmic stability for log K1–1 and the log K10 values of the complexes were measured and found to be -5.93 in acidic medium and -0.76 in alkaline medium, respectively. Pointed out that the adsorption of Cr(VI) on eucalyptus bark was greater than 90% in all cases, Cr(VI) recovery is strongly acidic dependent and shows max...
Un montón de detalles se encienden y se apagan, sin embargo, en mi memoria visual. Este pasaje re... more Un montón de detalles se encienden y se apagan, sin embargo, en mi memoria visual. Este pasaje revela la presencia de lo cinematográfico en la narrativa bryceana. Tanto la historia de Manongo como la aproximación misma del narrador, manifiestan una formación dentro de una cultura marcada por el cine, sus héroes, sus historias y, sobre todo, sus imágenes. La historia de amor
Vad kännetecknar SUHF-modellen respektive ABC-systemet? Hur är SUHF-modellen uppbyggd jämfört med... more Vad kännetecknar SUHF-modellen respektive ABC-systemet? Hur är SUHF-modellen uppbyggd jämfört med ABC-systemet? Syfte: Syftet med den här uppsatsen är att beskriva och analysera de skillnader och likheter som finns mellan SUHF-modellen och ABCsystemet. Metod: Studien var av kvalitativ karaktär och baserades på sekundärdata i form av vetenskapliga artiklar och böcker inom ämnet ABC-systemet i lärosäten. Som empiriskt underlag gjordes en semistrukturerad intervju med ekonomichefen på Mälardalens högskola för att få information om SUHF-modellen. Även rapporter framtagna av SUHF:s arbetsgrupp användes som underlag till den empiriska delen. Slutsats: Målet med de båda modellerna är att full kostnadstäckning ska ske genom att fördela de direkta och indirekta kostnaderna på ett tillfredställande vis till kostnadsobjektet/kostnadsbäraren. En tydlig skillnad som vi kan se mellan modellerna är att ABC-systemets fokus ligger på aktivitetsgrupper och aktiviteter medan SUHF-modellens fokus ligger på kärnverksamheten respektive stödverksamheten men att de i grund och botten är benämningar för samma utföranden. Vi anser slutligen att SUHF-modellen är ett tillräckligt effektivt kostnadssystem som är väl anpassat efter svenska lärosätens behov och som bör fortsätta att användas och utvecklas.
ABSTRACTAdenoviral infections in the immunocompromised host are associated with significant morbi... more ABSTRACTAdenoviral infections in the immunocompromised host are associated with significant morbidity and mortality. Although the adoptive transfer of adenovirus-specific T cells may prevent and treat such infections, the T-cell immune response to the multiplicity of adenovirus serotypes and subspecies that infect humans has not been well characterized, impeding the development of such approaches. We have, therefore, analyzed the specificities of T-cell responses to the viral capsid hexon antigen, since this structure is highly conserved in human pathogens. We screened 25 human cytotoxic T-cell lines with adenovirus specificity to extensively characterize their responses to adenoviral hexon and to identify a panel of novel CD4+and CD8+T-cell epitopes. Using a peptide library spanning the entire sequence of the hexon protein, we confirmed the responsiveness of these cytotoxic T-cell lines to seven peptides described previously and also identified 33 new CD4- or CD8-restricted hexon e...
Background: Gas Permeable Rapid Expansion (G-Rex) bioreactors have been shown to efficiently expa... more Background: Gas Permeable Rapid Expansion (G-Rex) bioreactors have been shown to efficiently expand immune cells intended for therapeutic use, but do not address the complexity of the viral transduction step required for many engineered T-cell products. Here we demonstrate a novel method for transduction of activated T cells with Vectofusin-1 reagent. Transduction is accomplished in suspension, in G-Rex bioreactors. The simplified transduction step is integrated into a streamlined process that uses a single bioreactor with limited operator intervention. Methods: Peripheral blood mononuclear cells (PBMCs) from healthy donors were thawed, washed and activated with soluble anti-CD3 and anti-CD28 antibodies either in cell culture bags or in G-Rex bioreactors. Cells were cultured in TexMACS GMP medium with interleukin (IL)-7 and IL-15 and transduced with RetroNectin in bags or Vectorfusin-1 in the G-Rex. Total viable cell number, fold expansion, viability, transduction efficiency, phenotype and function were compared between the two processes. Results: The simplified process uses a single vessel from activation through harvest and achieves 56% transduction with 29-fold expansion in 11 days. The cells generated in the simplified process do not differ from cells produced in the conventional bag-based process functionally or phenotypically. Discussion: This study demonstrates that T cells can be transduced in suspension. Further, the conventional method of generating engineered T cells in bags for clinical use can be streamlined to a much simpler, lessexpensive process without compromising the quality or function of the cell product.
Introduction: Genetically modified T cells are being investigated to treat a variety of disorders... more Introduction: Genetically modified T cells are being investigated to treat a variety of disorders and have been particularly successful in treating B cell cancers. As more effort is poured into new targets, molecular switches, and various other modifications, development of processes to quickly manufacture new products must keep up. Current manufacturing processes often require highly skilled operators and specialized equipment. Here, we demonstrate a simplified, novel method for transduction of T cells, followed by robust expansion in the G-Rex bioreactor with no need for intervention until harvest. A scaled-up, closed-version of the same process, including a closed harvest step with the GatheRex is currently under evaluation. Methods: Frozen peripheral blood mononuclear cells (PBMCs) from healthy donors were used as starting material. PBMCs were thawed, washed, and activated with soluble anti-CD3 and anti-CD28 antibodies either in cell culture bags (32-C, Saint-Gobain Cell Therapy...
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Papers by Mariam Khalil