Diagnostic and Interventional Radiology, Apr 1, 2009
Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical... more Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical, laboratory, or radiological sign of the disease. Thus, the diagnosis, especially of focal involvement, is usually difficult. We here report ultrasonography, computed tomography and magnetic resonance imaging findings of primary pancreatic Burkitt lymphoma in a case with unusual radiological presentation.
Translocation of bacteria from the gut into pancreatic necrosis is an important factor in the dev... more Translocation of bacteria from the gut into pancreatic necrosis is an important factor in the development of septic complications and mortality in acute pancreatitis. S-methylisothiourea (SMT) is an inducible nitric oxide synthase inhibitor that has been shown to decrease bacteria] translocation in sepsis and thermal injury. To investigate whether SMT could affect bacterial translocation in acute necrotizing pancreatitis. Forty-five Sprague-Dawley rats were studied. Acute pancreatitis was induced in Group I and Group II by injection of taurocholate and trypsin into the common biliopancreatic duct. Group III underwent laparotomy with the manipulation (but not cannulation) of the pancreas and received saline injection. Group I rats received normal saline as a placebo, and Group II rats received SMT after surgery for 2 days. At 48 hours, blood was drawn for serum amylase determinations. Bacterial translocation to mesenteric lymph nodes and distant sites (pancreas, liver, and peritoneum...
Physiological research / Academia Scientiarum Bohemoslovaca, 2003
Various protocols may be used for acute pancreatitis treatment. Recently, the benefit of hyperbar... more Various protocols may be used for acute pancreatitis treatment. Recently, the benefit of hyperbaric oxygen (HBO) has been demonstrated. To clarify the mechanism of HBO on the process of the acute pancreatitis, we determined the levels of antioxidant enzymes in an acute pancreatitis model. Forty-five Sprague-Dawley rats were randomly divided into three groups: Group I: sham group (n=15), Group II: pancreatitis group (n=15), Group III: pancreatitis group undergoing HBO therapy (n=15). HBO was applied postoperatively for 5 days, two sessions per day at 2.5 fold absolute atmospheric pressure (ATA) for 90 min. Superoxide dismutase (Cu/Zn-SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH Px) activity were measured in pancreatic tissue and erythrocyte lysate. MDA and GSH Px were also determined in plasma. In addition, amylase levels were measured in the serum. While serum amylase levels and MDA values in erythrocyte, plasma and pancreatic tissue were decreased, the levels of GSH...
World journal of gastroenterology : WJG, Jan 28, 2007
To evaluate ursodeoxycholic acid (UDCA) therapy on the in vitro contraction of gallbladder smooth... more To evaluate ursodeoxycholic acid (UDCA) therapy on the in vitro contraction of gallbladder smooth muscle strips from cholesterol gallstone patients. The contraction forces of gallbladder smooth muscle strips from 28 patients with cholesterol gallstones treated with UDCA were compared with contraction forces from 14 untreated patients. The strips were stimulated with increasing concentrations of cholecystokinin-8 (CCK-8). Although the contraction forces that developed in response to CCK-8 were higher in strips from specimens of UDCA treated patients compared to untreated patients, longer treatment periods (6-wk) caused more contraction responses than the short treatment period of 3-wk (F = 19.297, 1.85 +/- 0.22 g vs 1.70 +/- 0.10 g, P < 0.01). Contraction forces developed with maximal stimulation with KCl in the 6-wk treatment group were also higher than contraction forces in the untreated group (F = 4.274, 3.77 +/- 0.45 g vs 3.30 +/- 0.30 g, P < 0.05). Six-week UDCA treatment ...
Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation, 2005
Fulminant hepatic failure continues to be a challenge to hepatologists and surgeons. Because of t... more Fulminant hepatic failure continues to be a challenge to hepatologists and surgeons. Because of the rapid deterioration in the clinical condition of patients with fulminant hepatic failure and the scarcity of available grafts, an ABO-incompatible liver donor may be the only choice for a patient with life-threatening hepatic insufficiency. Here, we report a patient with fulminant hepatic failure who received an ABO-incompatible liver transplantation who was treated with pre- and posttransplantation double-volume total plasma exchange, splenectomy, and triple immunosuppression (tacrolimus, mycophenolate mofetil, and prednisone) in July 2003. At 26 months' follow-up, the patient's postoperative course has been uneventful. Using protocols aimed at removing existing isohemagglutinins and reducing further antibody production, ABO-incompatible liver transplantation may be performed as a life-saving procedure in patients with fulminant hepatic failure.
Reactive oxygen species and derivatives which are implicated in pathogenesis of pancreatitis are ... more Reactive oxygen species and derivatives which are implicated in pathogenesis of pancreatitis are a group of substances which may provide distribution of proenzymes following destruction of acinary cells and may activate lysosomal enzymes. We aimed to show that oxidative stress in rats rendered acute necrotizing pancreatitis experimentally. Twenty Spraque-Dawley rats were included into the study and they were divided into the subgroups of pancreatitis and controls. Taurocholate (3%) in doses of 1 mL/kg was administered into the biliopancreatic duct of the rats to render them with acute necrotizing pancreatitis. Rats were killed at 48 hours after the procedure. Malondealdehyde levels and superoxide dismutase activities in pancreatic tissue and erythrocytes were determined. Significantly elevated tissue and erythrocyte malondealdehyde levels were found in rats with pancreatitis (p < 0.001). Superoxide dismutase activities in both tissues and erythrocytes of the rats with pancreatiti...
Diagnostic and interventional radiology (Ankara, Turkey), 2009
Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical... more Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical, laboratory, or radiological sign of the disease. Thus, the diagnosis, especially of focal involvement, is usually difficult. We here report ultrasonography, computed tomography and magnetic resonance imaging findings of primary pancreatic Burkitt lymphoma in a case with unusual radiological presentation.
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 2009
One of the most important complications of acute pancreatitis is the secondary bacterial infectio... more One of the most important complications of acute pancreatitis is the secondary bacterial infections of the pancreas and gut. Translocation of bacteria from the gut is accepted as being responsible for the development of septic complications in acute pancreatitis. In this study, our aim was to investigate the effect of PARP inhibition via 3-aminobenzamide on the bacterial translocation in acute pancreatitis. 45 male Sprague-Dawley rats were randomly allocated into three groups. Group I (Sham+saline) received normal saline infusion into the common biliopancreatic duct. Acute pancreatitis was induced in Group II (acute pancreatitis+saline) and Group III (acute pancreatitis+ 3-aminobenzamide) by the retrograde injection of taurocholate into the common biliopancreatic duct. Six hours after induction of pancreatitis, the rats in Group I and II were treated with saline (1 ml, every 12 hours), while the rats in Group III were treated with 3-aminobenzamide (10 mg/kg/day every 12 hours), intr...
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology
There has been considerable interest in gall bladder motility in recent years. We compared the ef... more There has been considerable interest in gall bladder motility in recent years. We compared the effects of cholecystokinin (CCK) and erythromycin on bile chemistry and gallstone formation in aged guinea pigs. Two groups of guinea pigs (1-mo and 3-y old; n=40 each) were studied. Each group was divided into four subgroups of 10 animals each; one subgroup received lithogenic diet, one each received CCK or erythromycin daily in addition to lithogenic diet for 4 weeks, and one received normal diet. After 4 weeks, the presence of gallstones or sludge was recorded and bile composition including concentrations of bile acid, cholesterol, lecithin and protein concentrations was studied. No gallstones were observed in the 1-mo-old animals. In the 3-year-old animals, 9 of 10 guinea pigs on lithogenic diet and 4 of 10 in each treatment subgroup and the normal diet subgroup developed gallstones. CCK and erythromycin had similar effects on bile chemistry and stone formation. Aging increases the for...
Acute pancreatitis is a local inflammatory process that leads to a systemic inflammatory response... more Acute pancreatitis is a local inflammatory process that leads to a systemic inflammatory response in the majority of cases. Bacterial contamination has been estimated to occur in 30-40% of patients with necrotizing pancreatitis. Development of pancreatic necrosis depends mainly on the degree of inflammation and on the microvascular circulation of the pancreatic tissue. Activated protein C (APC) is known to inhibit coagulation and inflammation, and to promote fibrinolysis in patients with severe sepsis. We investigated the effects of APC on histopathology, bacterial translocation, and systemic inflammation in experimental acute necrotizing pancreatitis. Forty-five male Sprague-Dawley rats were studied. Rats were randomly allocated to three groups. Acute pancreatitis was induced in group II (positive control; n = 15) and group III (treatment; n = 15) rats by retrograde injection of taurocholate into the common biliopancreatic duct. Group I rats (sham; n = 15) received an injection of ...
To evaluate the organelle-based changes in acinar cells in experimental acute necrotizing pancrea... more To evaluate the organelle-based changes in acinar cells in experimental acute necrotizing pancreatitis (ANP) after taurine treatment and the association of electron microscopic findings with histopathological changes and oxidative stress markers. The study was performed in February 2005 at Gulhane School of Medicine and Hecettepe University, Turkey. Forty-five rats were divided into 3 groups. Acute necrotizing pancreatitis was induced in groups II and III. Groups I and II were treated with saline and Group III with taurine 1000 mg/kg/day, i.p, for 48 hours. Histopathological and ultrastructural examinations were determined using one-way analysis of variance and Kruskal-Wallis tests. Histopathologic findings improved significantly after taurine treatment. Degree of injury in rough and smooth endoplasmic reticulums, Golgi apparatus, mitochondria and nucleus of acinar cells also decreased with taurine in correlation with biochemical and histological results. Taurine improves acinar cel...
World journal of gastroenterology : WJG, Jan 21, 2007
To investigate the antifibrotic effects of peginterferon-alpha 2b and taurine on oxidative stress... more To investigate the antifibrotic effects of peginterferon-alpha 2b and taurine on oxidative stress markers and hepatocellular apoptosis. Sixty rats with CCl4-induced liver fibrosis were divided into 4 groups (n=15). Group 1 was left for spontaneous recovery (SR). Groups 2-4 received peginterferon-alpha 2b, taurine, and their combination, respectively, for four weeks. Histological fibrosis scores, histomorphometric analysis, tissue hydroxyproline, tissue MDA, GPx and SOD activities were determined. Activated stellate cells and hepatocellular apoptosis were also evaluated. The degree of fibrosis decreased in all treatment groups compared to spontaneous recovery group. Taurine alone and in combination with peginterferon-alpha 2b reduced oxidative stress markers, but peginterferon-alpha 2b alone did not. Apoptotic hepatocytes and activated stellate cells were higher in groups 2-4 than in group 1. Combined taurine and peginterferon-alpha 2b further reduced fibrosis and increased activated...
Diagnostic and Interventional Radiology, Apr 1, 2009
Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical... more Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical, laboratory, or radiological sign of the disease. Thus, the diagnosis, especially of focal involvement, is usually difficult. We here report ultrasonography, computed tomography and magnetic resonance imaging findings of primary pancreatic Burkitt lymphoma in a case with unusual radiological presentation.
Translocation of bacteria from the gut into pancreatic necrosis is an important factor in the dev... more Translocation of bacteria from the gut into pancreatic necrosis is an important factor in the development of septic complications and mortality in acute pancreatitis. S-methylisothiourea (SMT) is an inducible nitric oxide synthase inhibitor that has been shown to decrease bacteria] translocation in sepsis and thermal injury. To investigate whether SMT could affect bacterial translocation in acute necrotizing pancreatitis. Forty-five Sprague-Dawley rats were studied. Acute pancreatitis was induced in Group I and Group II by injection of taurocholate and trypsin into the common biliopancreatic duct. Group III underwent laparotomy with the manipulation (but not cannulation) of the pancreas and received saline injection. Group I rats received normal saline as a placebo, and Group II rats received SMT after surgery for 2 days. At 48 hours, blood was drawn for serum amylase determinations. Bacterial translocation to mesenteric lymph nodes and distant sites (pancreas, liver, and peritoneum...
Physiological research / Academia Scientiarum Bohemoslovaca, 2003
Various protocols may be used for acute pancreatitis treatment. Recently, the benefit of hyperbar... more Various protocols may be used for acute pancreatitis treatment. Recently, the benefit of hyperbaric oxygen (HBO) has been demonstrated. To clarify the mechanism of HBO on the process of the acute pancreatitis, we determined the levels of antioxidant enzymes in an acute pancreatitis model. Forty-five Sprague-Dawley rats were randomly divided into three groups: Group I: sham group (n=15), Group II: pancreatitis group (n=15), Group III: pancreatitis group undergoing HBO therapy (n=15). HBO was applied postoperatively for 5 days, two sessions per day at 2.5 fold absolute atmospheric pressure (ATA) for 90 min. Superoxide dismutase (Cu/Zn-SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH Px) activity were measured in pancreatic tissue and erythrocyte lysate. MDA and GSH Px were also determined in plasma. In addition, amylase levels were measured in the serum. While serum amylase levels and MDA values in erythrocyte, plasma and pancreatic tissue were decreased, the levels of GSH...
World journal of gastroenterology : WJG, Jan 28, 2007
To evaluate ursodeoxycholic acid (UDCA) therapy on the in vitro contraction of gallbladder smooth... more To evaluate ursodeoxycholic acid (UDCA) therapy on the in vitro contraction of gallbladder smooth muscle strips from cholesterol gallstone patients. The contraction forces of gallbladder smooth muscle strips from 28 patients with cholesterol gallstones treated with UDCA were compared with contraction forces from 14 untreated patients. The strips were stimulated with increasing concentrations of cholecystokinin-8 (CCK-8). Although the contraction forces that developed in response to CCK-8 were higher in strips from specimens of UDCA treated patients compared to untreated patients, longer treatment periods (6-wk) caused more contraction responses than the short treatment period of 3-wk (F = 19.297, 1.85 +/- 0.22 g vs 1.70 +/- 0.10 g, P < 0.01). Contraction forces developed with maximal stimulation with KCl in the 6-wk treatment group were also higher than contraction forces in the untreated group (F = 4.274, 3.77 +/- 0.45 g vs 3.30 +/- 0.30 g, P < 0.05). Six-week UDCA treatment ...
Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation, 2005
Fulminant hepatic failure continues to be a challenge to hepatologists and surgeons. Because of t... more Fulminant hepatic failure continues to be a challenge to hepatologists and surgeons. Because of the rapid deterioration in the clinical condition of patients with fulminant hepatic failure and the scarcity of available grafts, an ABO-incompatible liver donor may be the only choice for a patient with life-threatening hepatic insufficiency. Here, we report a patient with fulminant hepatic failure who received an ABO-incompatible liver transplantation who was treated with pre- and posttransplantation double-volume total plasma exchange, splenectomy, and triple immunosuppression (tacrolimus, mycophenolate mofetil, and prednisone) in July 2003. At 26 months' follow-up, the patient's postoperative course has been uneventful. Using protocols aimed at removing existing isohemagglutinins and reducing further antibody production, ABO-incompatible liver transplantation may be performed as a life-saving procedure in patients with fulminant hepatic failure.
Reactive oxygen species and derivatives which are implicated in pathogenesis of pancreatitis are ... more Reactive oxygen species and derivatives which are implicated in pathogenesis of pancreatitis are a group of substances which may provide distribution of proenzymes following destruction of acinary cells and may activate lysosomal enzymes. We aimed to show that oxidative stress in rats rendered acute necrotizing pancreatitis experimentally. Twenty Spraque-Dawley rats were included into the study and they were divided into the subgroups of pancreatitis and controls. Taurocholate (3%) in doses of 1 mL/kg was administered into the biliopancreatic duct of the rats to render them with acute necrotizing pancreatitis. Rats were killed at 48 hours after the procedure. Malondealdehyde levels and superoxide dismutase activities in pancreatic tissue and erythrocytes were determined. Significantly elevated tissue and erythrocyte malondealdehyde levels were found in rats with pancreatitis (p < 0.001). Superoxide dismutase activities in both tissues and erythrocytes of the rats with pancreatiti...
Diagnostic and interventional radiology (Ankara, Turkey), 2009
Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical... more Burkitt lymphoma predominantly involving the pancreas is very rare. There is no specific clinical, laboratory, or radiological sign of the disease. Thus, the diagnosis, especially of focal involvement, is usually difficult. We here report ultrasonography, computed tomography and magnetic resonance imaging findings of primary pancreatic Burkitt lymphoma in a case with unusual radiological presentation.
The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 2009
One of the most important complications of acute pancreatitis is the secondary bacterial infectio... more One of the most important complications of acute pancreatitis is the secondary bacterial infections of the pancreas and gut. Translocation of bacteria from the gut is accepted as being responsible for the development of septic complications in acute pancreatitis. In this study, our aim was to investigate the effect of PARP inhibition via 3-aminobenzamide on the bacterial translocation in acute pancreatitis. 45 male Sprague-Dawley rats were randomly allocated into three groups. Group I (Sham+saline) received normal saline infusion into the common biliopancreatic duct. Acute pancreatitis was induced in Group II (acute pancreatitis+saline) and Group III (acute pancreatitis+ 3-aminobenzamide) by the retrograde injection of taurocholate into the common biliopancreatic duct. Six hours after induction of pancreatitis, the rats in Group I and II were treated with saline (1 ml, every 12 hours), while the rats in Group III were treated with 3-aminobenzamide (10 mg/kg/day every 12 hours), intr...
Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology
There has been considerable interest in gall bladder motility in recent years. We compared the ef... more There has been considerable interest in gall bladder motility in recent years. We compared the effects of cholecystokinin (CCK) and erythromycin on bile chemistry and gallstone formation in aged guinea pigs. Two groups of guinea pigs (1-mo and 3-y old; n=40 each) were studied. Each group was divided into four subgroups of 10 animals each; one subgroup received lithogenic diet, one each received CCK or erythromycin daily in addition to lithogenic diet for 4 weeks, and one received normal diet. After 4 weeks, the presence of gallstones or sludge was recorded and bile composition including concentrations of bile acid, cholesterol, lecithin and protein concentrations was studied. No gallstones were observed in the 1-mo-old animals. In the 3-year-old animals, 9 of 10 guinea pigs on lithogenic diet and 4 of 10 in each treatment subgroup and the normal diet subgroup developed gallstones. CCK and erythromycin had similar effects on bile chemistry and stone formation. Aging increases the for...
Acute pancreatitis is a local inflammatory process that leads to a systemic inflammatory response... more Acute pancreatitis is a local inflammatory process that leads to a systemic inflammatory response in the majority of cases. Bacterial contamination has been estimated to occur in 30-40% of patients with necrotizing pancreatitis. Development of pancreatic necrosis depends mainly on the degree of inflammation and on the microvascular circulation of the pancreatic tissue. Activated protein C (APC) is known to inhibit coagulation and inflammation, and to promote fibrinolysis in patients with severe sepsis. We investigated the effects of APC on histopathology, bacterial translocation, and systemic inflammation in experimental acute necrotizing pancreatitis. Forty-five male Sprague-Dawley rats were studied. Rats were randomly allocated to three groups. Acute pancreatitis was induced in group II (positive control; n = 15) and group III (treatment; n = 15) rats by retrograde injection of taurocholate into the common biliopancreatic duct. Group I rats (sham; n = 15) received an injection of ...
To evaluate the organelle-based changes in acinar cells in experimental acute necrotizing pancrea... more To evaluate the organelle-based changes in acinar cells in experimental acute necrotizing pancreatitis (ANP) after taurine treatment and the association of electron microscopic findings with histopathological changes and oxidative stress markers. The study was performed in February 2005 at Gulhane School of Medicine and Hecettepe University, Turkey. Forty-five rats were divided into 3 groups. Acute necrotizing pancreatitis was induced in groups II and III. Groups I and II were treated with saline and Group III with taurine 1000 mg/kg/day, i.p, for 48 hours. Histopathological and ultrastructural examinations were determined using one-way analysis of variance and Kruskal-Wallis tests. Histopathologic findings improved significantly after taurine treatment. Degree of injury in rough and smooth endoplasmic reticulums, Golgi apparatus, mitochondria and nucleus of acinar cells also decreased with taurine in correlation with biochemical and histological results. Taurine improves acinar cel...
World journal of gastroenterology : WJG, Jan 21, 2007
To investigate the antifibrotic effects of peginterferon-alpha 2b and taurine on oxidative stress... more To investigate the antifibrotic effects of peginterferon-alpha 2b and taurine on oxidative stress markers and hepatocellular apoptosis. Sixty rats with CCl4-induced liver fibrosis were divided into 4 groups (n=15). Group 1 was left for spontaneous recovery (SR). Groups 2-4 received peginterferon-alpha 2b, taurine, and their combination, respectively, for four weeks. Histological fibrosis scores, histomorphometric analysis, tissue hydroxyproline, tissue MDA, GPx and SOD activities were determined. Activated stellate cells and hepatocellular apoptosis were also evaluated. The degree of fibrosis decreased in all treatment groups compared to spontaneous recovery group. Taurine alone and in combination with peginterferon-alpha 2b reduced oxidative stress markers, but peginterferon-alpha 2b alone did not. Apoptotic hepatocytes and activated stellate cells were higher in groups 2-4 than in group 1. Combined taurine and peginterferon-alpha 2b further reduced fibrosis and increased activated...
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