Four different tests to assess compost stability were compared during green waste composting in w... more Four different tests to assess compost stability were compared during green waste composting in windrows at a large-scale composting plant. The tests used were: germination index, (based on seed germination); SOUR (specific oxygen uptake rate, respirometric method based ...
Nitrogen availability plays a critical role in the biodegradation of organic matter during compos... more Nitrogen availability plays a critical role in the biodegradation of organic matter during composting. Although the optimal initial C/N is known to be around 25-30, the chemical form in which N is present influences microbial activity and therefore degradation rate and gaseous losses. This study was conducted to evaluate the influence of N availability on the composting of a mixture of lignocellulosic materials. Three composting piles were made of a mixture of wheat straw and cotton waste, each pile containing different N-rich animal by-products. The evolution of the main physico-chemical parameters was monitored (temperature, pH, electrical conductivity, C/N, NH(4)(+), NO(3)(-), water soluble C and N) as well as the enzymatic activity related to the cycle of the main nutrients (β-glucosidase, protease, alkaline phosphatase and fluorescein diacetate hydrolysis). Additionally, fluxes of CO(2), CH(4) and N(2)O emitted from the composting piles were measured by the closed-chamber technique. Cumulative CO(2) emissions were fitted to five different kinetic models with biological significance to C mineralization data. The application of the different N-rich residues had a significant effect on the C and N dynamics during composting. However, most enzymatic activities followed similar patterns in the three piles. The major CO(2) fluxes were recorded during the thermophilic phase, showing a direct relationship with temperature peaks. No CH(4) fluxes were detected for any of the composting piles during the whole trial, whereas low N(2)O emissions were found at the early beginning and during the maturation stage.
Extracted organic C and microbial biomass were evaluated as stability parameters in 3 different l... more Extracted organic C and microbial biomass were evaluated as stability parameters in 3 different ligno-cellulosic waste composts. Organic C was extracted by both water and alkali and further separated in humic-like carbon (HLC) and nonhumic carbon (NHC). Conventional humification parameters, such as humification index and degree of humification were calculated from NHC and HLC. Microbial biomass carbon (B(C)) was determined as an indicator of the degree of biochemical transformation, whereas ninhydrin reactive N (B(NIN)) was measured to obtain the stability parameter B(NIN)/N(TOT) (N(TOT), total N). The water-extracted organic C did not provide reliable information on the transformations underwent by the ligno-cellulosic wastes during composting, since its content remained almost unaltered during the whole process. In contrast, parameters based on the alkali-extracted organic C and microbial biomass clearly reflected organic matter (OM) changes during the process. There was an increase in the net amount of HLC in the alkali extracts throughout composting, especially in the first 7 to 12 wk of the process, as well as a relative enrichment of HLC with respect to NHC. Values of humification index and degree of humification in end products were consistent with an adequate level of compost stability. The stability parameter B(NIN)/N(TOT) showed to be a reliable indicator of stability in ligno-cellulosic wastes. Parameters based on the alkali-extracted C and microbial biomass clearly reflected the transformation of the OM during composting and can be used as stability parameters in ligno-cellulosic waste composts.
Four different tests to assess compost stability were compared during green waste composting in w... more Four different tests to assess compost stability were compared during green waste composting in windrows at a large-scale composting plant. The tests used were: germination index, (based on seed germination); SOUR (specific oxygen uptake rate, respirometric method based ...
Nitrogen availability plays a critical role in the biodegradation of organic matter during compos... more Nitrogen availability plays a critical role in the biodegradation of organic matter during composting. Although the optimal initial C/N is known to be around 25-30, the chemical form in which N is present influences microbial activity and therefore degradation rate and gaseous losses. This study was conducted to evaluate the influence of N availability on the composting of a mixture of lignocellulosic materials. Three composting piles were made of a mixture of wheat straw and cotton waste, each pile containing different N-rich animal by-products. The evolution of the main physico-chemical parameters was monitored (temperature, pH, electrical conductivity, C/N, NH(4)(+), NO(3)(-), water soluble C and N) as well as the enzymatic activity related to the cycle of the main nutrients (β-glucosidase, protease, alkaline phosphatase and fluorescein diacetate hydrolysis). Additionally, fluxes of CO(2), CH(4) and N(2)O emitted from the composting piles were measured by the closed-chamber technique. Cumulative CO(2) emissions were fitted to five different kinetic models with biological significance to C mineralization data. The application of the different N-rich residues had a significant effect on the C and N dynamics during composting. However, most enzymatic activities followed similar patterns in the three piles. The major CO(2) fluxes were recorded during the thermophilic phase, showing a direct relationship with temperature peaks. No CH(4) fluxes were detected for any of the composting piles during the whole trial, whereas low N(2)O emissions were found at the early beginning and during the maturation stage.
Extracted organic C and microbial biomass were evaluated as stability parameters in 3 different l... more Extracted organic C and microbial biomass were evaluated as stability parameters in 3 different ligno-cellulosic waste composts. Organic C was extracted by both water and alkali and further separated in humic-like carbon (HLC) and nonhumic carbon (NHC). Conventional humification parameters, such as humification index and degree of humification were calculated from NHC and HLC. Microbial biomass carbon (B(C)) was determined as an indicator of the degree of biochemical transformation, whereas ninhydrin reactive N (B(NIN)) was measured to obtain the stability parameter B(NIN)/N(TOT) (N(TOT), total N). The water-extracted organic C did not provide reliable information on the transformations underwent by the ligno-cellulosic wastes during composting, since its content remained almost unaltered during the whole process. In contrast, parameters based on the alkali-extracted organic C and microbial biomass clearly reflected organic matter (OM) changes during the process. There was an increase in the net amount of HLC in the alkali extracts throughout composting, especially in the first 7 to 12 wk of the process, as well as a relative enrichment of HLC with respect to NHC. Values of humification index and degree of humification in end products were consistent with an adequate level of compost stability. The stability parameter B(NIN)/N(TOT) showed to be a reliable indicator of stability in ligno-cellulosic wastes. Parameters based on the alkali-extracted C and microbial biomass clearly reflected the transformation of the OM during composting and can be used as stability parameters in ligno-cellulosic waste composts.
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Papers by Miguel Sánchez-monedero