Dr. Muhammad Salman is working as Assistant Professor in the Dept. of Microbiology and Biotechnology at Abasyn University Peshawar, Pakistan. He is HEC approved PhD supervisor in disciplines of Biological and Medical Science. He got his PhD degree in the field of infectious disease, vaccine development and protein science from National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan. He got special research training in vaccines at Human Therapeutic Portfolio, National Research Council (NRC), Canada, Along with 8 years research experience he his teaching to undergrads and MPhil students for last 3.5 years. Till now 8 research students completed their MPhil under supervision of Dr. Salman. He published more than 10 papers in prestigious journals with > 10 Impact factor and is serving as reviewer of reputed journals, moreover, he is editorial board member of American Journal of Clinical Microbiology and Antimicrobials, and Acta Microbiology. He established international collaborations with renowned scientists in developed countries. He was nominated for workshop by ICGEB, Italy from Pakistan. Dr. Salman is co-founder and CEO of Academy of Scientific Write-up and Presentation (ASWAP), Pakistan.
A continuous scanning laser Doppler vibrometry (CSLDV) obtained sweeping a single laser beam alon... more A continuous scanning laser Doppler vibrometry (CSLDV) obtained sweeping a single laser beam along a periodic scan pattern allows measuring surface vibrations at many points simultaneously by demultiplexing the CSLDV signal. This known method fundamentally differs from conventional scanning laser vibrometry techniques in which the laser beam is kept at a fixed point during each measurement and then moved to a new position prior to the next measurement. This article demonstrates the use of a CSLDV for measuring in a non-contact fashion the velocity of low-frequency surface waves (f < 100 Hz) propagating over soft materials, namely here gel surfaces—mimicking human body soft tissues—and skeletal muscles, to develop an affordable and noninvasive elastogra-phy modality. The CSLDV vibration measurements obtained with a single laser beam, linearly scanned over the test surface at 200 Hz over lengths up to 6 cm, were validated using an array of three fixed laser Doppler vibrometers distributed along the same scan line. Furthermore, this CSLDV setup was used to measure the increase in surface wave velocity over the biceps brachii muscle which was directly correlated to the actual stiffening of the biceps occurring while a subject was performing voluntary contractions at an increasing level.
Currently licensed typhoid vaccines are based on Vi capsular polysaccharides. Recent molecular re... more Currently licensed typhoid vaccines are based on Vi capsular polysaccharides. Recent molecular reports from typhoid endemic countries state that Salmonella enterica serovar Typhi (S. Typhi) Vi negative strains occur naturally and cause typhoid fever which is indistinguishable from disease caused by Vi positive strains. Vaccine based on Vi polysaccharide may not protect patients if the invading S. Typhi are negative for Vi. The lipopoly-saccharide (LPS) is an essential component of S. Typhi outer membrane in which O-specific polysaccharide (OSP) is a protective antigen and universal candidate for vaccine development. In this study, S. Typhi Vi negative isolates were discriminated from Vi positive isolates through a duplex PCR using primers of fliC-d (599bp) and tviA (495bp) genes. The LPS of S. Typhi Vi negative isolates was extracted by hot phenol method and OSP was purified by core hydrolysis. The yield of extracted LPS was 91 mg/L and that of purified OSP was 49.14 mg/L of culture broth. LPS showed ladder like appearance by zinc imidazole staining following SDS-PAGE. Whole cell challenged mice sera were used for in vitro antigenicity evaluation of the purified LPS and OSP. The antigenicity was found adequate by immunodiffusion assay. To our knowledge, this is the first report of purification and antigenic evaluation of LPS of a Vi negative S. Typhi isolate. The purified OSP from S. Typhi Vi negative isolate may be coupled with a carrier protein to produce universal low cost conjugate vaccine candidates for use in typhoid endemic regions.
2014 Cairo International Biomedical Engineering Conference, Dec 1, 2014
The development of automated systems for the detection and diagnosis of different diseases are qu... more The development of automated systems for the detection and diagnosis of different diseases are quite popular now-a-days. Hypertensive Retinopathy (HR) is an eye disease caused by high blood pressure. Formation of Cotton Wool Spots (CWS) is one of the important clinical signs that are seen at advanced stages in HR. An automated system is presented in this paper for the detection of Cotton Wool Spots in digital retinal images. The system first enhances candidate regions using Gabor filter bank, then binarize those regions using Global thresholding. The proposed system is tested on a locally gather fundus image dataset, and achieved 82.21% and 82.38% sensitivity and PPV, respectively.
A continuous scanning laser Doppler vibrometry (CSLDV) obtained sweeping a single laser beam alon... more A continuous scanning laser Doppler vibrometry (CSLDV) obtained sweeping a single laser beam along a periodic scan pattern allows measuring surface vibrations at many points simultaneously by demultiplexing the CSLDV signal. This known method fundamentally differs from conventional scanning laser vibrometry techniques in which the laser beam is kept at a fixed point during each measurement and then moved to a new position prior to the next measurement. This article demonstrates the use of a CSLDV for measuring in a non-contact fashion the velocity of low-frequency surface waves (f < 100 Hz) propagating over soft materials, namely here gel surfaces—mimicking human body soft tissues—and skeletal muscles, to develop an affordable and noninvasive elastogra-phy modality. The CSLDV vibration measurements obtained with a single laser beam, linearly scanned over the test surface at 200 Hz over lengths up to 6 cm, were validated using an array of three fixed laser Doppler vibrometers distributed along the same scan line. Furthermore, this CSLDV setup was used to measure the increase in surface wave velocity over the biceps brachii muscle which was directly correlated to the actual stiffening of the biceps occurring while a subject was performing voluntary contractions at an increasing level.
Currently licensed typhoid vaccines are based on Vi capsular polysaccharides. Recent molecular re... more Currently licensed typhoid vaccines are based on Vi capsular polysaccharides. Recent molecular reports from typhoid endemic countries state that Salmonella enterica serovar Typhi (S. Typhi) Vi negative strains occur naturally and cause typhoid fever which is indistinguishable from disease caused by Vi positive strains. Vaccine based on Vi polysaccharide may not protect patients if the invading S. Typhi are negative for Vi. The lipopoly-saccharide (LPS) is an essential component of S. Typhi outer membrane in which O-specific polysaccharide (OSP) is a protective antigen and universal candidate for vaccine development. In this study, S. Typhi Vi negative isolates were discriminated from Vi positive isolates through a duplex PCR using primers of fliC-d (599bp) and tviA (495bp) genes. The LPS of S. Typhi Vi negative isolates was extracted by hot phenol method and OSP was purified by core hydrolysis. The yield of extracted LPS was 91 mg/L and that of purified OSP was 49.14 mg/L of culture broth. LPS showed ladder like appearance by zinc imidazole staining following SDS-PAGE. Whole cell challenged mice sera were used for in vitro antigenicity evaluation of the purified LPS and OSP. The antigenicity was found adequate by immunodiffusion assay. To our knowledge, this is the first report of purification and antigenic evaluation of LPS of a Vi negative S. Typhi isolate. The purified OSP from S. Typhi Vi negative isolate may be coupled with a carrier protein to produce universal low cost conjugate vaccine candidates for use in typhoid endemic regions.
2014 Cairo International Biomedical Engineering Conference, Dec 1, 2014
The development of automated systems for the detection and diagnosis of different diseases are qu... more The development of automated systems for the detection and diagnosis of different diseases are quite popular now-a-days. Hypertensive Retinopathy (HR) is an eye disease caused by high blood pressure. Formation of Cotton Wool Spots (CWS) is one of the important clinical signs that are seen at advanced stages in HR. An automated system is presented in this paper for the detection of Cotton Wool Spots in digital retinal images. The system first enhances candidate regions using Gabor filter bank, then binarize those regions using Global thresholding. The proposed system is tested on a locally gather fundus image dataset, and achieved 82.21% and 82.38% sensitivity and PPV, respectively.
Uploads
Papers by Muhammad Salman