Twenty-five chipmunk species occur in the world, of which only the Siberian chipmunk, Tamias sibi... more Twenty-five chipmunk species occur in the world, of which only the Siberian chipmunk, Tamias sibiricus, inhabits Asia. To investigate mitochondrial cytochrome b sequence variations and population structure of the Siberian chipmunk in northeastern Asia, we examined mitochondrial cytochrome b sequences (1140 bp) from 3 countries. Analyses of 41 individuals from South Korea and 33 individuals from Russia and northeast China resulted in 37 haplotypes and 27 haplotypes, respectively. There were no shared haplotypes between South Korea and Russia--northeast China. Phylogenetic trees and network analysis showed 2 major maternal lineages for haplotypes, referred to as the S and R lineages. Haplotype grouping in each cluster was nearly coincident with its geographic affinity. In particular, 3 distinct groups were found that mostly clustered in the northern, central and southern parts of South Korea. Nucleotide diversity of the S lineage was twice that of lineage R. The divergence between S and R lineages was estimated to be 2.98-0.98 Myr. During the ice age, there may have been at least 2 refuges in South Korea and Russia--northeast China. The sequence variation between the S and R lineages was 11.3% (K2P), which is indicative of specific recognition in rodents. These results suggest that T. sibiricus from South Korea could be considered a separate species. However, additional information, such as details of distribution, nuclear genes data or morphology, is required to strengthen this hypothesis.
The taxonomic status and nomenclature of the golden lemming (Lemmus obensis chrysogaster) remaine... more The taxonomic status and nomenclature of the golden lemming (Lemmus obensis chrysogaster) remained a puzzle for more than 100 years. It was considered either as an independent species Lemmus chrysogaster or as a subspecies of Lemmus trimucronatus or Lemmus sibiricus. In the most authoritative checklists, without further evidence, it was transferred to the synonym of Lemmus amurensis. However, the type material and specimens from type locality have not been revised since 1903. Here we fill this gap. The sequence of the cytochrome b gene from topotype clearly indicates that the golden lemming is a younger synonym of L. trimucronatus.
The nematode Soboliphyme baturini Petrov, 1930, was found to represent a single species with a re... more The nematode Soboliphyme baturini Petrov, 1930, was found to represent a single species with a relatively broad geographic range across Beringia and northwestern North America on the basis of the assessment of molecular sequence data for adult and juvenile parasites. Refuted are hypotheses suggesting that several cryptic species could be partitioned either among an array of mustelid definitive hosts or across the vast region that links North America and Eurasia. Host specificity for this species is examined on the basis of a comprehensive list for definitive hosts, derived from new field surveys and existing literature for S. baturini. Only 5 mustelids (Gulo gulo, Martes americana, M. caurina, M. zibellina, and Neovison vison) appear to have significant roles in the life history, persistence, and transmission of this nematode. Soboliphyme baturini readily switches among M. americana, M. caurina, Mustela erminea, or N. vison at any particular locality throughout its geographic range ...
We analyse the phylogenetic relationships and taxonomic status of hymenolepidid cestodes with rud... more We analyse the phylogenetic relationships and taxonomic status of hymenolepidid cestodes with rudimentary rostellum, parasitic in Holarctic shrews of the genus Sorex. These cestodes were previously termed as a ‘Ditestolepis clade’ and encompass eight genera: Diorchilepis, Ditestolepis, Ecrinolepis, Gulyaevilepis, Mathevolepis, Novobrachylepis, Spalania and Spasskylepis. Representatives of seven of these were available for molecular study, including type species of all seven genera. Phylogenetic analysis based on partial sequences of the 28S rRNA gene supports this group of genera as monophyletic. The ‘Ditestolepis clade’ consists of two subclades. One of these includes genera containing cestodes having suckers positioned inside bothrium-like depressions (Ditestolepis, Gulyaevilepis and Spasskylepis). The second subclade contains cestodes with suckers expanding far beyond the margins of the scolex (Mathevolepis, Ecrinolepis, Spalania and Novobrachylepis). The relative taxonomic value...
Historical DNA of museum specimens is of paramount importance in elucidation of complex nomenclat... more Historical DNA of museum specimens is of paramount importance in elucidation of complex nomenclature issues and a priceless source of material gathered during preceding centuries in hardly accessible today sites. Here we report the results of genotyping type specimens of Lemmus obensis bungei Vinogradov, 1925 from the collection of the Zoological Institute RAS and specimens of lemmings from the upper streams of the Omolon River, identified as L. amurensis from the collection of the Institute of Systematics and Ecology of Animals, Siberian branch of RAS. The genetic profile of the type specimens in the first case was crucial for the nomenclatural solution as these specimens are from the point of the major evolutionary divergence on the Lena River. The results of the study convincingly showed that the lectotype belong to the eastern mitochondrial lineage of L. sibiricus. Therefore, if ever in the further studies it would be conclusively shown that this mitochondrial lineage may become...
The aim of this study was to assess the taxonomic diversity and prevalence of shrew cestodes on t... more The aim of this study was to assess the taxonomic diversity and prevalence of shrew cestodes on the Kamchatka Peninsula and Paramushir Island on the basis of current understanding of cestode taxonomy. The diversity of shrew cestodes included 22 species from three families: Hymenolepididae, Dilepididae, and Mesocestoididae. In Paramushir, the diversity of shrew cestodes was substantially lower than in Kamchatka (14 and 22, respectively). In Kamchatka, three genera of cestodes (Novobrachylepis, Mathevolepis, Ditestolepis) were not found, but three species from genera Lineolepis, Staphylocystis, and Monocercus are possibly new species. The potential for endemic species and the unique characteristics of the cestode fauna on Kamchatka suggest that the peninsula may have been a refugium for shrews and their parasites in the last glacial maximum. In Kamchatka and Paramushir, two Nearctic species (Lineolepis parva and L. pribilofensis) were found, which indicates potential dispersal from No...
Rock-dwelling pikas of the genus Ochotona have fragmented montane geographical distribution presu... more Rock-dwelling pikas of the genus Ochotona have fragmented montane geographical distribution presumably associated with geological and climatic events of Late Quaternary. Within this genus, the northern pika, O. hyperborea has the widest distribution. Taxonomic composition and subspecific structure of this species has been controversial during the past century. Sixteen available names were combined in different manners into a variable number of weakly overlapping subspecies by previous authors. A comparison of data sets from the entire distribution range of northern pikas is presented here for the first time, including: craniometric, bioacoustic, and multilocus genetic data. We examined 301 skulls, and the mitochondrial COI gene and two nuclear introns in 79 specimens, as well as the structure of alarm calls from 107 individuals. Our results show that the six subspecies within O. hyperborea correspond to the six genetic parapatric lineages. Five of these are hypothesized to be involv...
The data on allozyme variability of some genetic biochemical markers of field mouse, that is a re... more The data on allozyme variability of some genetic biochemical markers of field mouse, that is a recent newcomer in the territory of Magadan Region, are compared with those of probable donors from other areas. A significant genetic variability is reported for studied samplings in the majority of analyzed loci. Since any significant loss in genetic variability is not reported for the newly inhabited areas, this field mouse association is assumed to have been a result of their repeated introduction from the southern areas of the Russian Far East. The obtained study results still do not allow us to unequivocally establish the provenance area of the Magadan «enclave» of this species.
This paper presents a revised description of Ecrinolepis ezoensis (Sawada et Koyasu, 1995) comb. ... more This paper presents a revised description of Ecrinolepis ezoensis (Sawada et Koyasu, 1995) comb. n. in shrew Sorex caecutiens Laxmann, 1788 (type host) from Hokkaido, Japan. The first morphological description of E. ezoensis (Sawada et Koyasu, 1995) is completed with a number of new important characters including the cestode strobila differentiating into androgynous and male segments, threelobed ovary, vaginal sphincter and lacking internal seminal vesicle. The last character, together with scolex morphology as bothria-like suckers and lacking rhynchus and rhynchus vagina with rostellum available, are the basic features of Ditestolepidini tribe (Gulyaev, Lykova, 2005). The E. ezoensis is specified by its taxonomic status in Ditestolepidini tribe.
... Tyumen region, vicinity of Tobol'sk 2 ... burg), L. Emelyanova (Moscow), V. Lebedev ... more ... Tyumen region, vicinity of Tobol'sk 2 ... burg), L. Emelyanova (Moscow), V. Lebedev (Moscow), A. Pilni-kov (Krasnokamensk), A. Myslenkov and I. Voloshina (Lazo), K. Peleshko (Lazo), L. Levik (Moscow), N. Pozhidaeva (Terney), A. Obolenskiy (Novomoskowsk), Tae-Young Choi ...
Twenty-five chipmunk species occur in the world, of which only the Siberian chipmunk, Tamias sibi... more Twenty-five chipmunk species occur in the world, of which only the Siberian chipmunk, Tamias sibiricus, inhabits Asia. To investigate mitochondrial cytochrome b sequence variations and population structure of the Siberian chipmunk in northeastern Asia, we examined mitochondrial cytochrome b sequences (1140 bp) from 3 countries. Analyses of 41 individuals from South Korea and 33 individuals from Russia and northeast China resulted in 37 haplotypes and 27 haplotypes, respectively. There were no shared haplotypes between South Korea and Russia--northeast China. Phylogenetic trees and network analysis showed 2 major maternal lineages for haplotypes, referred to as the S and R lineages. Haplotype grouping in each cluster was nearly coincident with its geographic affinity. In particular, 3 distinct groups were found that mostly clustered in the northern, central and southern parts of South Korea. Nucleotide diversity of the S lineage was twice that of lineage R. The divergence between S and R lineages was estimated to be 2.98-0.98 Myr. During the ice age, there may have been at least 2 refuges in South Korea and Russia--northeast China. The sequence variation between the S and R lineages was 11.3% (K2P), which is indicative of specific recognition in rodents. These results suggest that T. sibiricus from South Korea could be considered a separate species. However, additional information, such as details of distribution, nuclear genes data or morphology, is required to strengthen this hypothesis.
The taxonomic status and nomenclature of the golden lemming (Lemmus obensis chrysogaster) remaine... more The taxonomic status and nomenclature of the golden lemming (Lemmus obensis chrysogaster) remained a puzzle for more than 100 years. It was considered either as an independent species Lemmus chrysogaster or as a subspecies of Lemmus trimucronatus or Lemmus sibiricus. In the most authoritative checklists, without further evidence, it was transferred to the synonym of Lemmus amurensis. However, the type material and specimens from type locality have not been revised since 1903. Here we fill this gap. The sequence of the cytochrome b gene from topotype clearly indicates that the golden lemming is a younger synonym of L. trimucronatus.
The nematode Soboliphyme baturini Petrov, 1930, was found to represent a single species with a re... more The nematode Soboliphyme baturini Petrov, 1930, was found to represent a single species with a relatively broad geographic range across Beringia and northwestern North America on the basis of the assessment of molecular sequence data for adult and juvenile parasites. Refuted are hypotheses suggesting that several cryptic species could be partitioned either among an array of mustelid definitive hosts or across the vast region that links North America and Eurasia. Host specificity for this species is examined on the basis of a comprehensive list for definitive hosts, derived from new field surveys and existing literature for S. baturini. Only 5 mustelids (Gulo gulo, Martes americana, M. caurina, M. zibellina, and Neovison vison) appear to have significant roles in the life history, persistence, and transmission of this nematode. Soboliphyme baturini readily switches among M. americana, M. caurina, Mustela erminea, or N. vison at any particular locality throughout its geographic range ...
We analyse the phylogenetic relationships and taxonomic status of hymenolepidid cestodes with rud... more We analyse the phylogenetic relationships and taxonomic status of hymenolepidid cestodes with rudimentary rostellum, parasitic in Holarctic shrews of the genus Sorex. These cestodes were previously termed as a ‘Ditestolepis clade’ and encompass eight genera: Diorchilepis, Ditestolepis, Ecrinolepis, Gulyaevilepis, Mathevolepis, Novobrachylepis, Spalania and Spasskylepis. Representatives of seven of these were available for molecular study, including type species of all seven genera. Phylogenetic analysis based on partial sequences of the 28S rRNA gene supports this group of genera as monophyletic. The ‘Ditestolepis clade’ consists of two subclades. One of these includes genera containing cestodes having suckers positioned inside bothrium-like depressions (Ditestolepis, Gulyaevilepis and Spasskylepis). The second subclade contains cestodes with suckers expanding far beyond the margins of the scolex (Mathevolepis, Ecrinolepis, Spalania and Novobrachylepis). The relative taxonomic value...
Historical DNA of museum specimens is of paramount importance in elucidation of complex nomenclat... more Historical DNA of museum specimens is of paramount importance in elucidation of complex nomenclature issues and a priceless source of material gathered during preceding centuries in hardly accessible today sites. Here we report the results of genotyping type specimens of Lemmus obensis bungei Vinogradov, 1925 from the collection of the Zoological Institute RAS and specimens of lemmings from the upper streams of the Omolon River, identified as L. amurensis from the collection of the Institute of Systematics and Ecology of Animals, Siberian branch of RAS. The genetic profile of the type specimens in the first case was crucial for the nomenclatural solution as these specimens are from the point of the major evolutionary divergence on the Lena River. The results of the study convincingly showed that the lectotype belong to the eastern mitochondrial lineage of L. sibiricus. Therefore, if ever in the further studies it would be conclusively shown that this mitochondrial lineage may become...
The aim of this study was to assess the taxonomic diversity and prevalence of shrew cestodes on t... more The aim of this study was to assess the taxonomic diversity and prevalence of shrew cestodes on the Kamchatka Peninsula and Paramushir Island on the basis of current understanding of cestode taxonomy. The diversity of shrew cestodes included 22 species from three families: Hymenolepididae, Dilepididae, and Mesocestoididae. In Paramushir, the diversity of shrew cestodes was substantially lower than in Kamchatka (14 and 22, respectively). In Kamchatka, three genera of cestodes (Novobrachylepis, Mathevolepis, Ditestolepis) were not found, but three species from genera Lineolepis, Staphylocystis, and Monocercus are possibly new species. The potential for endemic species and the unique characteristics of the cestode fauna on Kamchatka suggest that the peninsula may have been a refugium for shrews and their parasites in the last glacial maximum. In Kamchatka and Paramushir, two Nearctic species (Lineolepis parva and L. pribilofensis) were found, which indicates potential dispersal from No...
Rock-dwelling pikas of the genus Ochotona have fragmented montane geographical distribution presu... more Rock-dwelling pikas of the genus Ochotona have fragmented montane geographical distribution presumably associated with geological and climatic events of Late Quaternary. Within this genus, the northern pika, O. hyperborea has the widest distribution. Taxonomic composition and subspecific structure of this species has been controversial during the past century. Sixteen available names were combined in different manners into a variable number of weakly overlapping subspecies by previous authors. A comparison of data sets from the entire distribution range of northern pikas is presented here for the first time, including: craniometric, bioacoustic, and multilocus genetic data. We examined 301 skulls, and the mitochondrial COI gene and two nuclear introns in 79 specimens, as well as the structure of alarm calls from 107 individuals. Our results show that the six subspecies within O. hyperborea correspond to the six genetic parapatric lineages. Five of these are hypothesized to be involv...
The data on allozyme variability of some genetic biochemical markers of field mouse, that is a re... more The data on allozyme variability of some genetic biochemical markers of field mouse, that is a recent newcomer in the territory of Magadan Region, are compared with those of probable donors from other areas. A significant genetic variability is reported for studied samplings in the majority of analyzed loci. Since any significant loss in genetic variability is not reported for the newly inhabited areas, this field mouse association is assumed to have been a result of their repeated introduction from the southern areas of the Russian Far East. The obtained study results still do not allow us to unequivocally establish the provenance area of the Magadan «enclave» of this species.
This paper presents a revised description of Ecrinolepis ezoensis (Sawada et Koyasu, 1995) comb. ... more This paper presents a revised description of Ecrinolepis ezoensis (Sawada et Koyasu, 1995) comb. n. in shrew Sorex caecutiens Laxmann, 1788 (type host) from Hokkaido, Japan. The first morphological description of E. ezoensis (Sawada et Koyasu, 1995) is completed with a number of new important characters including the cestode strobila differentiating into androgynous and male segments, threelobed ovary, vaginal sphincter and lacking internal seminal vesicle. The last character, together with scolex morphology as bothria-like suckers and lacking rhynchus and rhynchus vagina with rostellum available, are the basic features of Ditestolepidini tribe (Gulyaev, Lykova, 2005). The E. ezoensis is specified by its taxonomic status in Ditestolepidini tribe.
... Tyumen region, vicinity of Tobol'sk 2 ... burg), L. Emelyanova (Moscow), V. Lebedev ... more ... Tyumen region, vicinity of Tobol'sk 2 ... burg), L. Emelyanova (Moscow), V. Lebedev (Moscow), A. Pilni-kov (Krasnokamensk), A. Myslenkov and I. Voloshina (Lazo), K. Peleshko (Lazo), L. Levik (Moscow), N. Pozhidaeva (Terney), A. Obolenskiy (Novomoskowsk), Tae-Young Choi ...
Uploads
Papers by Nikolai Dokuchaev