5TH INTERNATIONAL CONFERENCE ON ELECTRICAL, ELECTRONIC, COMMUNICATION AND CONTROL ENGINEERING (ICEECC 2021)
Candida albicans is a fungus that exists as a commensal constituent of the human microbiome and a... more Candida albicans is a fungus that exists as a commensal constituent of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen frequently occurs in the mucosa or endothelium associated with candidiasis. Econazole nitrate is an antifungal cream commonly used to treat C. albicans infection, however, little is known about its mode of action against C. albicans biofilm. Therefore, the present study was performed to investigate the mode of action of econazole nitrate against C. albicans biofilm by determining the change in biochemical composition of the biofilm. Biofilm biomass and viability in the presence of econazole nitrate were assessed using crystal violet and resazurin assay, respectively. Biochemical composition of the biofilm was determined by Fourier transform infrared (FTIR) spectroscopy. The results demonstrated that econazole nitrate substantially inhibited the biomass (52% - 68%) and viability (10% - 16%) of C. albicans biofilm. Typical FTIR spectrum of C. albicans biofilm showed a total of 12 peaks in the range between 1800 cm-1 - 600 cm-1. Treatment with econazole nitrate was found to alter the FTIR spectral peaks at 625 cm-1 (O=C–N bending), 720 cm-1 (C=C bending), 762 cm-1 (C-H bending), 817 cm-1 (α-mannans), 920 cm-1 (C=C bending), 1017 cm-1 (β-glucans), 1220 cm-1 (PO2-asymmetric stretching), 1375 cm-1 (N-H bending vibration), 1465 cm-1 (C-C ring stretching), 1534 cm-1 (CONH bending), 1625 cm-1 (C=O stretching), and 1735 cm-1 (C=O stretching). Our findings suggest that econazole nitrate may inhibit C. albicans biofilm by modifying its biochemical composition.
Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome ... more Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen occurs mostly in the mucosa or endothelium associated with candidiasis and colonizes medical devices. The present work was performed to determine the efficacy of the antifungal creams on the viability and biochemical composition of C. albicans biofilm. Four commercial antifungal creams were used herein namely econazole nitrate, miconazole nitrate, ketoconazole and tolnaftate. Resazurin assay and Fourier transform infrared (FTIR) spectroscopy were performed to determine the viability and biochemical composition of C. albicans biofilm, respectively. Results demonstrated that the antifungal creams inhibited C. albicans biofilm. The highest percent inhibition shown by econazole nitrate, miconazole nitrate, ketoconazole, and tolnaftate were 16.5%, 17.1%, 15.8%, and 6.9%, respectively. Econazole nitrate with the lowest IC...
Abstract With the increasing demand for large-area graphene due to its versatility, there is an i... more Abstract With the increasing demand for large-area graphene due to its versatility, there is an imminent requirement for scalable, low-temperature, and high yield growth procedures. In this study, the fabrication of large-area graphene nanoplatelets directly grown on tungsten (W) nanoparticles coated c-Si and quartz substrates by hot-wire chemical vapour deposition was demonstrated. A large area of single and bilayer graphene grown over W adatoms via controlled argon (Ar) plasma treatment varied from 0.5 to 10 min. The finest quality of continuous graphene layer up to an area of 2.56 × 104 μm2 was prepared at the optimised condition of 1 min, and verified through transmission electron microscopy in conjunction with energy dispersive X-ray, atomic force microscopy, and Raman spectroscopy. The prepared thin film of the carbon layer has excellent optical transparency (> 70%) and lower sheet resistance up to 718.3 Ω/sq. A detailed growth mechanism is proposed for the nucleation of graphene nanoplatelets under the influence of Ar plasma treatment on W nanoparticles.
2008 IEEE International Conference on Semiconductor Electronics, 2008
... Nurul Hidayah Mohamad Norl, Suhaimi Taib2, Ibrahim Ahmad3, Member, IEEE and Huda Abdullahl, M... more ... Nurul Hidayah Mohamad Norl, Suhaimi Taib2, Ibrahim Ahmad3, Member, IEEE and Huda Abdullahl, Member, IEEE lDepartment of Electric, Electronic and System Faculty of Engineering 43600 ... Low profile Quad Flat package (LQFP) with ultra Fine pitch bond pad were used. ...
2007 5th Student Conference on Research and Development, 2007
Abstract This study analyzes the most fundamental constraint of RFID systems that is power rectif... more Abstract This study analyzes the most fundamental constraint of RFID systems that is power rectification. This issue plays an important role in the development of long range RFID systems especially the passive RFID system. Rectifiers are the key components in power ...
The term "microbial biofilm" refers to three-dimensional, stationary populations of microorganism... more The term "microbial biofilm" refers to three-dimensional, stationary populations of microorganisms comprising various cell types encased in a matrix primarily produced by the microbial community. In biofilm research, selecting the appropriate experimental platform is crucial, as it determines the data gathered and, consequently, impacts the quality of the studies. Biofilm biomass and viability can be assessed using the crystal violet assay and resazurin assay, respectively. Due to its convenience and adaptability, the 96-well microplate is frequently employed in various scientific and medical applications. Various characterization techniques for biofilm assays using the 6-well microplate include light microscopy, confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), atomic force microscopy (AFM), and FTIR (Fourier-transform infrared) spectroscopy. This mini review discusses the measurement of biofilm biomass and viability, experimental protocols for crystal violet and resazurin assays, and potential issues in microplate-based biofilm assays.
5TH INTERNATIONAL CONFERENCE ON ELECTRICAL, ELECTRONIC, COMMUNICATION AND CONTROL ENGINEERING (ICEECC 2021)
Candida albicans is a fungus that exists as a commensal constituent of the human microbiome and a... more Candida albicans is a fungus that exists as a commensal constituent of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen frequently occurs in the mucosa or endothelium associated with candidiasis. Econazole nitrate is an antifungal cream commonly used to treat C. albicans infection, however, little is known about its mode of action against C. albicans biofilm. Therefore, the present study was performed to investigate the mode of action of econazole nitrate against C. albicans biofilm by determining the change in biochemical composition of the biofilm. Biofilm biomass and viability in the presence of econazole nitrate were assessed using crystal violet and resazurin assay, respectively. Biochemical composition of the biofilm was determined by Fourier transform infrared (FTIR) spectroscopy. The results demonstrated that econazole nitrate substantially inhibited the biomass (52% - 68%) and viability (10% - 16%) of C. albicans biofilm. Typical FTIR spectrum of C. albicans biofilm showed a total of 12 peaks in the range between 1800 cm-1 - 600 cm-1. Treatment with econazole nitrate was found to alter the FTIR spectral peaks at 625 cm-1 (O=C–N bending), 720 cm-1 (C=C bending), 762 cm-1 (C-H bending), 817 cm-1 (α-mannans), 920 cm-1 (C=C bending), 1017 cm-1 (β-glucans), 1220 cm-1 (PO2-asymmetric stretching), 1375 cm-1 (N-H bending vibration), 1465 cm-1 (C-C ring stretching), 1534 cm-1 (CONH bending), 1625 cm-1 (C=O stretching), and 1735 cm-1 (C=O stretching). Our findings suggest that econazole nitrate may inhibit C. albicans biofilm by modifying its biochemical composition.
Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome ... more Candida albicans is a microbial fungus that exists as a commensal member of the human microbiome and an opportunistic pathogen. Biofilm formation by this fungal pathogen occurs mostly in the mucosa or endothelium associated with candidiasis and colonizes medical devices. The present work was performed to determine the efficacy of the antifungal creams on the viability and biochemical composition of C. albicans biofilm. Four commercial antifungal creams were used herein namely econazole nitrate, miconazole nitrate, ketoconazole and tolnaftate. Resazurin assay and Fourier transform infrared (FTIR) spectroscopy were performed to determine the viability and biochemical composition of C. albicans biofilm, respectively. Results demonstrated that the antifungal creams inhibited C. albicans biofilm. The highest percent inhibition shown by econazole nitrate, miconazole nitrate, ketoconazole, and tolnaftate were 16.5%, 17.1%, 15.8%, and 6.9%, respectively. Econazole nitrate with the lowest IC...
Abstract With the increasing demand for large-area graphene due to its versatility, there is an i... more Abstract With the increasing demand for large-area graphene due to its versatility, there is an imminent requirement for scalable, low-temperature, and high yield growth procedures. In this study, the fabrication of large-area graphene nanoplatelets directly grown on tungsten (W) nanoparticles coated c-Si and quartz substrates by hot-wire chemical vapour deposition was demonstrated. A large area of single and bilayer graphene grown over W adatoms via controlled argon (Ar) plasma treatment varied from 0.5 to 10 min. The finest quality of continuous graphene layer up to an area of 2.56 × 104 μm2 was prepared at the optimised condition of 1 min, and verified through transmission electron microscopy in conjunction with energy dispersive X-ray, atomic force microscopy, and Raman spectroscopy. The prepared thin film of the carbon layer has excellent optical transparency (> 70%) and lower sheet resistance up to 718.3 Ω/sq. A detailed growth mechanism is proposed for the nucleation of graphene nanoplatelets under the influence of Ar plasma treatment on W nanoparticles.
2008 IEEE International Conference on Semiconductor Electronics, 2008
... Nurul Hidayah Mohamad Norl, Suhaimi Taib2, Ibrahim Ahmad3, Member, IEEE and Huda Abdullahl, M... more ... Nurul Hidayah Mohamad Norl, Suhaimi Taib2, Ibrahim Ahmad3, Member, IEEE and Huda Abdullahl, Member, IEEE lDepartment of Electric, Electronic and System Faculty of Engineering 43600 ... Low profile Quad Flat package (LQFP) with ultra Fine pitch bond pad were used. ...
2007 5th Student Conference on Research and Development, 2007
Abstract This study analyzes the most fundamental constraint of RFID systems that is power rectif... more Abstract This study analyzes the most fundamental constraint of RFID systems that is power rectification. This issue plays an important role in the development of long range RFID systems especially the passive RFID system. Rectifiers are the key components in power ...
The term "microbial biofilm" refers to three-dimensional, stationary populations of microorganism... more The term "microbial biofilm" refers to three-dimensional, stationary populations of microorganisms comprising various cell types encased in a matrix primarily produced by the microbial community. In biofilm research, selecting the appropriate experimental platform is crucial, as it determines the data gathered and, consequently, impacts the quality of the studies. Biofilm biomass and viability can be assessed using the crystal violet assay and resazurin assay, respectively. Due to its convenience and adaptability, the 96-well microplate is frequently employed in various scientific and medical applications. Various characterization techniques for biofilm assays using the 6-well microplate include light microscopy, confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), atomic force microscopy (AFM), and FTIR (Fourier-transform infrared) spectroscopy. This mini review discusses the measurement of biofilm biomass and viability, experimental protocols for crystal violet and resazurin assays, and potential issues in microplate-based biofilm assays.
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