The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 2015
Our objective was to determine whether there were significant differences in genome-wide DNA meth... more Our objective was to determine whether there were significant differences in genome-wide DNA methylation in newborns with major congenital heart defect (CHD) compared to controls. We also evaluated methylation of cytosines in CpG motifs for the detection of these CHDs. Genome-wide DNA methylation analysis was performed on DNA from 60 newborns with various CHDs, including hypoplastic left heart syndrome, ventricular septal deficit, atrial septal defect, pulmonary stenosis, coarctation of the aorta and Tetralogy of Fallot, and 32 controls. Highly significant differences in cytosine methylation were seen in a large number of genes throughout the genome for all CHD categories. Gene ontology analysis of CHD overall indicated over-represented biological processes involving cell development and differentiation, and anatomical structure morphogenesis. Methylation of individual cytosines in CpG motifs had high diagnostic accuracy for the detection of CHD. For example, for coarctation one pre...
Summary The study was conducted to determine whether preoperative serum levels of cancer antigen ... more Summary The study was conducted to determine whether preoperative serum levels of cancer antigen (CA) 125, CA15- 3, CA19-9, carcinoembryonic antigen (CEA), and alpha-fetoprotein (AFP) are associated clinicopathologically with poor prognostic parameters and adjuvant treatment requirements in women with pure endometrioid endometrial cancer (EEC). The authors performed a retrospective review of EEC cases that were treated between January 2008 and January 2011. The association between preoperative tumor markers and prognostic parameters, recurrence risk, and adjuvant treatment requirements were investigated. Following univariate analyses, receiver-operating characteristic (ROC) curves were constructed for each marker to assess their capacity to predict prognostic parameters and need for adjuvant treatment. A total of 166 EEC cases were identified. Mean CA125, CA15-3, and CA19-9 levels were higher in cases that required adjuvant treatment (p < 0.05). CA125 had significant power for pr...
American journal of obstetrics and gynecology, Jan 23, 2015
To perform validation studies of previously published and newly derived first-trimester metabolom... more To perform validation studies of previously published and newly derived first-trimester metabolomic algorithms for prediction of early preeclampsia (early-PE). Nuclear Magnetic Resonance (NMR)-based metabolomic analysis was performed on the first trimester serum in 50 women who subsequently developed early-PE and 108 first trimester controls. Random stratification and allocation was used to divide cases into a discovery group (30 early-PE and 65 controls) for generation of the biomarker model(s) and a validation group (20 early-PE and 43 controls) to ensure an unbiased assessment of the predictive algorithms. Cross Validation testing on the different algorithms was performed to confirm their robustness before use. Metabolites, demographic features, clinical characteristics and uterine Doppler pulsatility index (UtPI) data were evaluated. Area under the receiver operator characteristic curve (AUC), 95% CI, sensitivity and specificity of the biomarker models were derived. Validation t...
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 2015
Our objective was to determine whether there were significant differences in genome-wide DNA meth... more Our objective was to determine whether there were significant differences in genome-wide DNA methylation in newborns with major congenital heart defect (CHD) compared to controls. We also evaluated methylation of cytosines in CpG motifs for the detection of these CHDs. Genome-wide DNA methylation analysis was performed on DNA from 60 newborns with various CHDs, including hypoplastic left heart syndrome, ventricular septal deficit, atrial septal defect, pulmonary stenosis, coarctation of the aorta and Tetralogy of Fallot, and 32 controls. Highly significant differences in cytosine methylation were seen in a large number of genes throughout the genome for all CHD categories. Gene ontology analysis of CHD overall indicated over-represented biological processes involving cell development and differentiation, and anatomical structure morphogenesis. Methylation of individual cytosines in CpG motifs had high diagnostic accuracy for the detection of CHD. For example, for coarctation one pre...
Summary The study was conducted to determine whether preoperative serum levels of cancer antigen ... more Summary The study was conducted to determine whether preoperative serum levels of cancer antigen (CA) 125, CA15- 3, CA19-9, carcinoembryonic antigen (CEA), and alpha-fetoprotein (AFP) are associated clinicopathologically with poor prognostic parameters and adjuvant treatment requirements in women with pure endometrioid endometrial cancer (EEC). The authors performed a retrospective review of EEC cases that were treated between January 2008 and January 2011. The association between preoperative tumor markers and prognostic parameters, recurrence risk, and adjuvant treatment requirements were investigated. Following univariate analyses, receiver-operating characteristic (ROC) curves were constructed for each marker to assess their capacity to predict prognostic parameters and need for adjuvant treatment. A total of 166 EEC cases were identified. Mean CA125, CA15-3, and CA19-9 levels were higher in cases that required adjuvant treatment (p < 0.05). CA125 had significant power for pr...
American journal of obstetrics and gynecology, Jan 23, 2015
To perform validation studies of previously published and newly derived first-trimester metabolom... more To perform validation studies of previously published and newly derived first-trimester metabolomic algorithms for prediction of early preeclampsia (early-PE). Nuclear Magnetic Resonance (NMR)-based metabolomic analysis was performed on the first trimester serum in 50 women who subsequently developed early-PE and 108 first trimester controls. Random stratification and allocation was used to divide cases into a discovery group (30 early-PE and 65 controls) for generation of the biomarker model(s) and a validation group (20 early-PE and 43 controls) to ensure an unbiased assessment of the predictive algorithms. Cross Validation testing on the different algorithms was performed to confirm their robustness before use. Metabolites, demographic features, clinical characteristics and uterine Doppler pulsatility index (UtPI) data were evaluated. Area under the receiver operator characteristic curve (AUC), 95% CI, sensitivity and specificity of the biomarker models were derived. Validation t...
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Papers by Onur Turkoglu