T. LACOMBE1), L. AUDEGUIN2), M. BOSELLI3), B. BUCCHETTI4), F. CABELLO5), P. CHATELET1), M. CRESPA... more T. LACOMBE1), L. AUDEGUIN2), M. BOSELLI3), B. BUCCHETTI4), F. CABELLO5), P. CHATELET1), M. CRESPAN6), C. D’ONOFRIO7), J. EIRAS DIAS8), S. ERCISLI9), M. GARDIMAN6), M. S. GRANDO10), S. IMAZIO11), O. JANDUROVA12), A. JUNG13), E. KISS14), P. KOZMA15), E. MAUL16), D. MAGHRADZE17), M. C. MARTINEZ18), G. MUNOZ5), J. K. PATKOVA19), I. PEJIC20), E. PETERLUNGER4), D. PITSOLI21), D. PREINER20), S. RAIMONDI22), F. REGNER23), G. SAVIN24), S. SAVVIDES25), A. SCHNEIDER22), J. L. SPRING26), A. SZOKE14), A. VERES14), J. M. BOURSIQUOT1), R. BACILIERI1) and P. THIS1)
The creating of the 'ideal cultivar' appeared in th e second part of the 19 th century, w... more The creating of the 'ideal cultivar' appeared in th e second part of the 19 th century, which has resistance to downy mildew and powdery mildew combined with the quality of European varieties. The resistance breeding to fungal pathog ens grapevine was based on Vitis species that are autochthonous in North-America, and underwent a coevolution with the pathogen. Despite of
Biotechnology, biotechnological equipment, Jan 2, 2014
Twenty-seven grapevine (Vitis vinifera L.) varieties within 12 putative berry colour variation gr... more Twenty-seven grapevine (Vitis vinifera L.) varieties within 12 putative berry colour variation groups (conculta) were genotyped with 14 highly polymorphic microsatellite (simple sequence repeats (SSR)) markers. Three additional oligonucleotide primers were applied for the detection of the Gret1 retroelement insertion in the promoter region of VvMybA1 transcription factor gene regulating the UFGT (UDP-glucose: flavonoid 3-O-glucosyltransferase) activity. UFGT is the key enzyme of the anthocyanin biosynthetic pathway. SSR results proved that the analysed cultivars can be grouped only into nine concultas, the other three putative berry colour variant groups consist of homonyms as a consequence of misnaming. In the case of Sárfehér-Sárpiros, Delaware red-Delaware white and Járdovány fekete-Járdovány fehér, it was attested that they are not bud sports, but homonyms. Some conculta members could be differentiated according to the presence or the absence of the Gret1 retroelement (Chasselas...
Molecular marker-assisted selection (MAS) was carried out to track the inheritance of Run1 and Re... more Molecular marker-assisted selection (MAS) was carried out to track the inheritance of Run1 and Ren1, two dominant powdery mildew resistance genes that originate from different genetic resources. The Run1 locus was introgressed from a Muscadinia rotundifolia×Vitis vinifera BC4 hybrid plant derived from a recurrent pseudo-backcross breeding scheme, whereas the Ren1 locus was introgressed from the powdery mildew-resistant V. vinifera L.
Agrobacteria are efficient plant pathogens. They are able to transform plant cells genetically re... more Agrobacteria are efficient plant pathogens. They are able to transform plant cells genetically resulting in abnormal cell proliferation. Cultivars of Vitis vinifera are highly susceptible to many virulent Agrobacterium strains but certain wild Vitis species, including Vitis amurensis have resistant genotypes. Studies of the molecular background of such natural resistance are of special importance, not only for practical benefits in agricultural practice but also for understanding the role of plant genes in the transformation process. Earlier, crown gall resistance from V. amurensis was introgressed into V. vinifera through interspecific breeding and it was shown to be inherited as a single and dominant Mendelian trait. To develop this research further, towards understanding underlying molecular mechanisms, a mapping population was established, and resistance-coupled molecular DNA markers were identified by three different approaches. First, RAPD makers linked to the resistance locus (Rcg1) were identified, and on the basis of their DNA sequences, we developed resistance-coupled SCAR markers. However, localization of these markers in the grapevine genome sequence failed due to their similarity to many repetitive regions. Next, using SSR markers of the grapevine reference linkage map, location of the resistance locus was established on linkage group 15 (LG15). Finally, this position was supported further by developing new chromosome-specific markers and by the construction of the genetic map of the region including nine loci in 29.1 cM. Our results show that the closest marker is located 3.3 cM from the Rcg1 locus that may correspond to 576 kb.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012
The Rpv3 locus is a major determinant of downy mildew resistance in grapevine (Vitis spp.). A sel... more The Rpv3 locus is a major determinant of downy mildew resistance in grapevine (Vitis spp.). A selective sweep at this locus was revealed by the DNA genotyping of 580 grapevines, which include a highly diverse set of 265 European varieties that predated the spread of North American mildews, 82 accessions of wild species, and 233 registered breeding lines with North American ancestry produced in the past 150 years. Artificial hybridisation and subsequent phenotypic selection favoured a few Rpv3 haplotypes that were introgressed from wild vines and retained in released varieties. Seven conserved haplotypes in five descent groups of resistant varieties were traced back to their founders: (1) 'Munson', a cross between two of Hermann Jaeger's selections of V. rupestris and V. lincecumii made in the early 1880s in Missouri, (2) V. rupestris 'Ganzin', first utilised for breeding in 1879 by Victor Ganzin in France, (3) 'Noah', selected in 1869 from intermin...
T. LACOMBE1), L. AUDEGUIN2), M. BOSELLI3), B. BUCCHETTI4), F. CABELLO5), P. CHATELET1), M. CRESPA... more T. LACOMBE1), L. AUDEGUIN2), M. BOSELLI3), B. BUCCHETTI4), F. CABELLO5), P. CHATELET1), M. CRESPAN6), C. D’ONOFRIO7), J. EIRAS DIAS8), S. ERCISLI9), M. GARDIMAN6), M. S. GRANDO10), S. IMAZIO11), O. JANDUROVA12), A. JUNG13), E. KISS14), P. KOZMA15), E. MAUL16), D. MAGHRADZE17), M. C. MARTINEZ18), G. MUNOZ5), J. K. PATKOVA19), I. PEJIC20), E. PETERLUNGER4), D. PITSOLI21), D. PREINER20), S. RAIMONDI22), F. REGNER23), G. SAVIN24), S. SAVVIDES25), A. SCHNEIDER22), J. L. SPRING26), A. SZOKE14), A. VERES14), J. M. BOURSIQUOT1), R. BACILIERI1) and P. THIS1)
The creating of the 'ideal cultivar' appeared in th e second part of the 19 th century, w... more The creating of the 'ideal cultivar' appeared in th e second part of the 19 th century, which has resistance to downy mildew and powdery mildew combined with the quality of European varieties. The resistance breeding to fungal pathog ens grapevine was based on Vitis species that are autochthonous in North-America, and underwent a coevolution with the pathogen. Despite of
Biotechnology, biotechnological equipment, Jan 2, 2014
Twenty-seven grapevine (Vitis vinifera L.) varieties within 12 putative berry colour variation gr... more Twenty-seven grapevine (Vitis vinifera L.) varieties within 12 putative berry colour variation groups (conculta) were genotyped with 14 highly polymorphic microsatellite (simple sequence repeats (SSR)) markers. Three additional oligonucleotide primers were applied for the detection of the Gret1 retroelement insertion in the promoter region of VvMybA1 transcription factor gene regulating the UFGT (UDP-glucose: flavonoid 3-O-glucosyltransferase) activity. UFGT is the key enzyme of the anthocyanin biosynthetic pathway. SSR results proved that the analysed cultivars can be grouped only into nine concultas, the other three putative berry colour variant groups consist of homonyms as a consequence of misnaming. In the case of Sárfehér-Sárpiros, Delaware red-Delaware white and Járdovány fekete-Járdovány fehér, it was attested that they are not bud sports, but homonyms. Some conculta members could be differentiated according to the presence or the absence of the Gret1 retroelement (Chasselas...
Molecular marker-assisted selection (MAS) was carried out to track the inheritance of Run1 and Re... more Molecular marker-assisted selection (MAS) was carried out to track the inheritance of Run1 and Ren1, two dominant powdery mildew resistance genes that originate from different genetic resources. The Run1 locus was introgressed from a Muscadinia rotundifolia×Vitis vinifera BC4 hybrid plant derived from a recurrent pseudo-backcross breeding scheme, whereas the Ren1 locus was introgressed from the powdery mildew-resistant V. vinifera L.
Agrobacteria are efficient plant pathogens. They are able to transform plant cells genetically re... more Agrobacteria are efficient plant pathogens. They are able to transform plant cells genetically resulting in abnormal cell proliferation. Cultivars of Vitis vinifera are highly susceptible to many virulent Agrobacterium strains but certain wild Vitis species, including Vitis amurensis have resistant genotypes. Studies of the molecular background of such natural resistance are of special importance, not only for practical benefits in agricultural practice but also for understanding the role of plant genes in the transformation process. Earlier, crown gall resistance from V. amurensis was introgressed into V. vinifera through interspecific breeding and it was shown to be inherited as a single and dominant Mendelian trait. To develop this research further, towards understanding underlying molecular mechanisms, a mapping population was established, and resistance-coupled molecular DNA markers were identified by three different approaches. First, RAPD makers linked to the resistance locus (Rcg1) were identified, and on the basis of their DNA sequences, we developed resistance-coupled SCAR markers. However, localization of these markers in the grapevine genome sequence failed due to their similarity to many repetitive regions. Next, using SSR markers of the grapevine reference linkage map, location of the resistance locus was established on linkage group 15 (LG15). Finally, this position was supported further by developing new chromosome-specific markers and by the construction of the genetic map of the region including nine loci in 29.1 cM. Our results show that the closest marker is located 3.3 cM from the Rcg1 locus that may correspond to 576 kb.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2012
The Rpv3 locus is a major determinant of downy mildew resistance in grapevine (Vitis spp.). A sel... more The Rpv3 locus is a major determinant of downy mildew resistance in grapevine (Vitis spp.). A selective sweep at this locus was revealed by the DNA genotyping of 580 grapevines, which include a highly diverse set of 265 European varieties that predated the spread of North American mildews, 82 accessions of wild species, and 233 registered breeding lines with North American ancestry produced in the past 150 years. Artificial hybridisation and subsequent phenotypic selection favoured a few Rpv3 haplotypes that were introgressed from wild vines and retained in released varieties. Seven conserved haplotypes in five descent groups of resistant varieties were traced back to their founders: (1) 'Munson', a cross between two of Hermann Jaeger's selections of V. rupestris and V. lincecumii made in the early 1880s in Missouri, (2) V. rupestris 'Ganzin', first utilised for breeding in 1879 by Victor Ganzin in France, (3) 'Noah', selected in 1869 from intermin...
Uploads
Papers by Pál Kozma