Awarded Ph.D in Biotechnology. My research mainly focuses on Biotechnology, cancer biology, Bioinformatics and Molecularbiology techniques. I have published over 14 papers in several prestigious peer-reviewed journals, 1 book chapter in Elsevier and 1 in Springer publisher's, 2 books in Lambert publisher's and has presented more than 20 abstracts at various national and international conferences. I have life membership in Indian Science Congress Association and the American Association of Cancer Research (AACR). Phone: 9030244198 Address: #301, 65 Amaltas, Vasanth Vihar, phokhran road #2, THANE WEST-400610 Maharashtra
To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabe... more To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabean, the present study was intended to evaluate the in-vitro immunomodulatory activity of dry seeds of Glycine max. L. Effect of ethanolic and methanolic extracts of soyabean was evaluated at various concentrations (800μg/ml to 6.25μg/ml) for secretion of various mediators. Immunomodulatory activity of both extracts was examined on murine macrophage phagocytosis by Nitroblue tetrazolium (NBT) dye reduction, nitric oxide, lysosomal enzyme activity and myeloperoxidase activity. Proliferation of spleenocytes and bone marrow cells was evaluated by 3 (4, 5-dimethylthiazole-2-yl)-2, 5diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) as mitogen. Both aqueous and acetone extracts presented immunomodulatory activity in peritoneal mouse macrophages, bone marrow cells and spleenocytes without clear dose response relationship. In-vitro phagocytic index and proliferatio...
Exploring Pancreatic Metabolism and Malignancy, 2019
Pancreatic cancer (PC) is a lethal, malignant cancer that bears high mortality rates. Due to its ... more Pancreatic cancer (PC) is a lethal, malignant cancer that bears high mortality rates. Due to its lack of noticeable symptoms, it is often diagnosed late and current pancreatic cancer therapies are ineffective with poor prognosis. Despite the high mortality and poor survival of PC patients, there is limited information on factors propagating resistance. Resistance to standard therapies in pancreatic cancer patients is partly associated with the presence of a subpopulation of highly plastic “stem-like” cells (pancreatic cancer stem cells: paCSC) in tumors. In this connection, it is important to have a strong understanding of the paCSC population, especially its distinct characteristics, in order to engineer new therapies to target these resistant cells. Therefore, the purpose of this investigation is to highlight and discuss PaCSC and their specific surface markers. Overall, in this study, we searched MEDLINE, EMBASE, the Cochrane Library, Web of Science, and ISI Proceedings for observational studies relating to the PaCSC and PC. Pancreatic cancer stem cells exhibit specific immune characteristics on their surface. The CD133, CD44, CD24, ALDH1, c-Met, DCLK1, CXCR4, EpCAM and ABCG2 are prominent Pa-CSC markers. As PaCSCs drive tumorigenesis and metastasis, their manipulation approaches would have widespread clinical implications and hence improve outcomes in pancreatic cancer.
Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and has a high ... more Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and has a high fatality rate. Genetic and epigenetic aberrations are commonly observed in HCC. The epigenetic processes include chromatin remodelling, histone alterations, DNA methylation, and noncoding RNA (ncRNA) expression and are connected with the progression and metastasis of HCC. Due to their potential reversibility, these epigenetic alterations are widely targeted for the development of biomarkers. In-depth understanding of the epigenetics of HCC is critical for developing rational clinical strategies that can provide a meaningful improvement in overall survival and prediction of therapeutic outcomes. In this article, we have summarised the epigenetic modifications involved in HCC progression and highlighted the potential biomarkers for diagnosis and drug development.
Microbiology is a basic and scientific knowledge for Problem-solving in humans from different pat... more Microbiology is a basic and scientific knowledge for Problem-solving in humans from different pathogens. In order to this, a thorough knowledge of microbiology is essential to know exactly which microbes cause diseases. This manual provides a clear knowledge for the isolation of microbes from the different sources, preparation of nutrient supplement, culturing of microorganisms and preservative techniques in laboratories. This manual is very helpful for graduates students, teachers, and researchers. The experiments have been given in chronological order and most of these can be performed with materials available in biology laboratories. For some of the experiments, even alternate procedures have been suggested. The experiments covered in this manual are easy to perform and have been used over the years in our laboratory for imparting practical training regularly to undergraduates and postgraduate students.Experiments were written in a step-wise manner for easy understanding.
Pancreatic cancer ranks as the fourth cause of cancer-associated deaths in the West. It is an agg... more Pancreatic cancer ranks as the fourth cause of cancer-associated deaths in the West. It is an aggressive cancer with poor prognosis and a huge mortality rate. Current treatment modalities used for treating PC (surgery and chemoradiotherapy) have limited outcomes due to stroma-induced chemoresistance. These shortcomings stress the need for new therapeutic solutions with increased efficacy and cytotoxicity for PC. In recent years, nanotechnology has emerged as a promising solution for improving diagnosis, drug delivery, and treatment in many cancers including PC. In this chapter, we explore the various nanotechnologies developed to date, their chemical composition, pharmacological properties, and activity. We also discuss their potential as drug delivery solutions for PC.
Colorectal cancer (CRC) is the most prevalent cancer around the world, and advancements in therap... more Colorectal cancer (CRC) is the most prevalent cancer around the world, and advancements in therapy continue to reduce illness and death. CRC examination and therapy with various nanotechnology-based methods have led to promising results, under scientific panel to establish novel therapeutic strategies. Today, the use of nanoparticles as a drug delivery has become the most auspicious form of cancer therapy. Many studies have revealed a keen benefit of phytochemicals and nanoparticles combined therapy for various cancers. In this chapter, we ensure a deeper discussion regarding the latest advancement of board nanoparticle drug-based phytochemical fabrication against colorectal cancer detection and treatment.
Cancer theranostics represents a strategy that aims at combining diagnosis with therapy through t... more Cancer theranostics represents a strategy that aims at combining diagnosis with therapy through the simultaneous imaging and targeted delivery of therapeutics to cancer cells. Recently, the folate receptor alpha has emerged as an attractive theranostic target due to its overexpression in multiple solid tumors and its great functional versatility. In fact, it can be incorporated into folate-conjugated nano-systems for imaging and drug delivery. Hence, it can be used along the line of personalized clinical strategies as both an imaging tool and a delivery method ensuring the selective transport of treatments to tumor cells, thus highlighting its theranostic qualities. In this review, we will explore these theranostic characteristics in detail and assess their clinical potential. We will also discuss the technological advances that have allowed the design of sophisticated folate-based nanocarriers harboring various chemical properties and suited for the transport of various therapeutic agents.
Female-specific cancers are the most common cancers in women worldwide. Early detection methods r... more Female-specific cancers are the most common cancers in women worldwide. Early detection methods remain unavailable for most of these cancers, signifying that most of them are diagnosed at later stages. Furthermore, current treatment options for most female-specific cancers are surgery, radiation and chemotherapy. Although important milestones in molecularly targeted approaches have been achieved lately, current therapeutic strategies for female-specific cancers remain limited, ineffective and plagued by the emergence of chemoresistance, which aggravates prognosis. Recently, the application of nanotechnology to the medical field has allowed the development of novel nano-based approaches for the management and treatment of cancers, including female-specific cancers. These approaches promise to improve patient survival rates by reducing side effects, enabling selective delivery of drugs to tumor tissues and enhancing the uptake of therapeutic compounds, thus increasing anti-tumor activity. In this review, we focus on the application of nano-based technologies to the design of novel and innovative diagnostic and therapeutic strategies in the context of female-specific cancers, highlighting their potential uses and limitations.
Aquaporins (AQPs) are hydrophobic integral trans-membrane channel proteins implicated in cellular... more Aquaporins (AQPs) are hydrophobic integral trans-membrane channel proteins implicated in cellular proliferation and water transport in various cancers. They compose a family of 13 different isoforms (25-34 kDa) in mammals that have been identified to date. These AQPs exhibit a unique pattern of tissue expression. Though they can be found in most tissues, they are mostly active in endothelial and epithelial tissues. In this review, the AQPs associated with female-specific cancers are comprehensively explored and their significant features are analysed. Recent findings revealing their role in female-specific carcinogenesis, especially cervical, ovarian, uterine/endometrial, and breast, will be also discussed.
The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune sy... more The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune system in healthy cells. However, it is upregulated in various malignancies, involved in cancer development and is also immunogenic. Furthermore, FRα is a tumor-associated antigen endowed with unique properties, thus rendering it a suitable target for immunotherapeutic development in cancer. Various anti- FRα immunotherapeutic strategies are thus currently being developed and clinically assessed for the treatment of various solid tumors. These approaches include passive anti-FRα immunotherapies, such as monoclonal antibodies, or active immunotherapies, such as CART, folate haptens and vaccines. In this review, we will explore the advances in the field of FRα-based immune therapies and discuss both their successes and shortcomings in the clinical setting.
The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune sy... more The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune system in healthy cells. However, it is upregulated in various malignancies, involved in cancer development and is also immunogenic. Furthermore, FRα is a tumor-associated antigen endowed with unique properties, thus rendering it a suitable target for immunotherapeutic development in cancer. Various anti- FRα immunotherapeutic strategies are thus currently being developed and clinically assessed for the treatment of various solid tumors. These approaches include passive anti-FRα immunotherapies, such as monoclonal antibodies, or active immunotherapies, such as CART, folate haptens and vaccines. In this review, we will explore the advances in the field of FRα-based immune therapies and discuss both their successes and shortcomings in the clinical setting.
The present study was carried out to isolation of yeast and production of ethanol using papaya ... more The present study was carried out to isolation of yeast and production of ethanol using papaya (Carica papaya) and grape (Vitis vinifera) pulps with isolated yeast. Using selective medium, the yeast strains were isolated from fruit pulps and differential tests were applied for identification of the yeast. Percentage of yield of ethanol and effect of different nutrient supplements for the production of ethanol was studied using isolated yeast as synthetic medium is control. The reducing sugar was also estimated after 72 hrs saccharification, the amount of reducing sugar increased significantly (14.6 g/100g) in papaya fruit than grape fruit pulp (10.3 g/ 100g) and it is evident that high amount of carbohydrate levels. The fermented papaya yielded (6.20%) more ethanol content than grape pulp (3.83%). Addition of carbohydrate supplement, sucrose and nitrogen supplement of soya meal produced high yield of ethanol with yeast in papaya (11.45% & 9.16 %) than grape pulp (7.61% & 6.03%). The present study revealed that the fully ripened papaya fruit is a good source for ethanol production and it is possibility of ethanol production from our locally available fruits using simple, cheap, and adaptive technology with biochemically characterized yeast isolated from fruit pulps
The present investigation was carried out to study the anti-bacterial activity of ethanolic
and m... more The present investigation was carried out to study the anti-bacterial activity of ethanolic and methanolic extracts of stem, berries and whole plant of Solanum nigrum. The antibacterial activity was detected against Bacillus subtilis, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Solanum nigrum plant extracts of, 10ìg, 50ìg and 100ìg were taken as per CLSI standards. The zones of inhibitions obtained were recorded and analyzed against standard control streptomycin. The methanolic extracts were showed highest antibacterial activity than ethanolic extracts. In both extracts, whole plant extract showed potential anti-bacterial activity than stem and berries. The results indicate that the whole plant extracts of Solanum nigrum may recommend to use in preparation of herbal drugs. ISSN:-2
Objective: Personal health practices have an important role in promotion of oral health of an ind... more Objective: Personal health practices have an important role in promotion of oral health of an individual. Dental diseases are irreversible, damage cannot be reverted. The knowledge among the people is of pivotal importance as these diseases cannot be identified at initial stages. Raising public awareness about dental check up may assist in early diagnosis. This study aims at finding out oral health knowledge and oral health behavior among Tirupati students. Methods: A structured Questionnaire was distributed to Post graduate students of Tirupati (N=190) during the academic year 2011-12. The respondents have filled in the forms in their class itself and ample time was given to respond. The questionnaire consisted information on general background, oral practices and oral health knowledge. Results: Oral health knowledge seemed to be very limited. More than half of the students didn’t visit a dentist for almost 2 years now. Only few knew the importance of fluorides in caries prevention. Very less proportion practiced dental flossing and used mouth washes. Only 10% of study group knew that a father could transmit S.mutans to the child. 68.42% knew that extraction is the not only possible treatment. Only 10.53% of the study group could attain a score of 5 but none could score the maximum points. Conclusion: It can be concluded that students had limited knowledge on oral hygiene and ideal oral hygiene practices. Their brushing practices are still far behind the international recommendation of twice a day. The establishment and strengthening of oral hygiene education, including the importance of tooth brushing for prevention of periodontal disease, has been proposed.
The present study was aimed to investigate the antibacterial activity of aqueous, methanolic and ... more The present study was aimed to investigate the antibacterial activity of aqueous, methanolic and ethanolic
extracts of root, fruit and whole plant of Cephalendra indica using disc diffusion method. The antibacterial
activity was detected against Gram-positive and Gram-negative bacteria, Bacillus subtilis, Bacillus ceresu,
Staphylococus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Different
solvent extracts of root, fruit and whole plant of Cephalendra indica was taken in 50,100,150 and 200 μg as
per CLSI standards. The zones of inhibitions obtained were recorded and analyzed against standard
controls, Streptomycin and Ampicillin. The methanolic extracts of fruit and whole plant were showed
highest antibacterial activity than ethanolic and aqueous extracts. The higher concentration of methanolic
extract of whole plant, maximum antibacterial activity was observed against to Gram-negative bacteria than
Gram-positive bacteria. When compared with fruit and root extracts of different solvents, methanolic
extract of whole plant exhibit high antibacterial activity. The results indicate that Cephalendra indica
whole plant also has potent antibacterial activity similar to fruit and leaves.
To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabe... more To explore the potentially health modulatory role of most proteinaceous leguminosae plant, Soyabean, the present study was intended to evaluate the in-vitro immunomodulatory activity of dry seeds of Glycine max. L. Effect of ethanolic and methanolic extracts of soyabean was evaluated at various concentrations (800μg/ml to 6.25μg/ml) for secretion of various mediators. Immunomodulatory activity of both extracts was examined on murine macrophage phagocytosis by Nitroblue tetrazolium (NBT) dye reduction, nitric oxide, lysosomal enzyme activity and myeloperoxidase activity. Proliferation of spleenocytes and bone marrow cells was evaluated by 3 (4, 5-dimethylthiazole-2-yl)-2, 5diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) as mitogen. Both aqueous and acetone extracts presented immunomodulatory activity in peritoneal mouse macrophages, bone marrow cells and spleenocytes without clear dose response relationship. In-vitro phagocytic index and proliferatio...
Exploring Pancreatic Metabolism and Malignancy, 2019
Pancreatic cancer (PC) is a lethal, malignant cancer that bears high mortality rates. Due to its ... more Pancreatic cancer (PC) is a lethal, malignant cancer that bears high mortality rates. Due to its lack of noticeable symptoms, it is often diagnosed late and current pancreatic cancer therapies are ineffective with poor prognosis. Despite the high mortality and poor survival of PC patients, there is limited information on factors propagating resistance. Resistance to standard therapies in pancreatic cancer patients is partly associated with the presence of a subpopulation of highly plastic “stem-like” cells (pancreatic cancer stem cells: paCSC) in tumors. In this connection, it is important to have a strong understanding of the paCSC population, especially its distinct characteristics, in order to engineer new therapies to target these resistant cells. Therefore, the purpose of this investigation is to highlight and discuss PaCSC and their specific surface markers. Overall, in this study, we searched MEDLINE, EMBASE, the Cochrane Library, Web of Science, and ISI Proceedings for observational studies relating to the PaCSC and PC. Pancreatic cancer stem cells exhibit specific immune characteristics on their surface. The CD133, CD44, CD24, ALDH1, c-Met, DCLK1, CXCR4, EpCAM and ABCG2 are prominent Pa-CSC markers. As PaCSCs drive tumorigenesis and metastasis, their manipulation approaches would have widespread clinical implications and hence improve outcomes in pancreatic cancer.
Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and has a high ... more Hepatocellular carcinoma (HCC) is the most common primary malignancy of the liver and has a high fatality rate. Genetic and epigenetic aberrations are commonly observed in HCC. The epigenetic processes include chromatin remodelling, histone alterations, DNA methylation, and noncoding RNA (ncRNA) expression and are connected with the progression and metastasis of HCC. Due to their potential reversibility, these epigenetic alterations are widely targeted for the development of biomarkers. In-depth understanding of the epigenetics of HCC is critical for developing rational clinical strategies that can provide a meaningful improvement in overall survival and prediction of therapeutic outcomes. In this article, we have summarised the epigenetic modifications involved in HCC progression and highlighted the potential biomarkers for diagnosis and drug development.
Microbiology is a basic and scientific knowledge for Problem-solving in humans from different pat... more Microbiology is a basic and scientific knowledge for Problem-solving in humans from different pathogens. In order to this, a thorough knowledge of microbiology is essential to know exactly which microbes cause diseases. This manual provides a clear knowledge for the isolation of microbes from the different sources, preparation of nutrient supplement, culturing of microorganisms and preservative techniques in laboratories. This manual is very helpful for graduates students, teachers, and researchers. The experiments have been given in chronological order and most of these can be performed with materials available in biology laboratories. For some of the experiments, even alternate procedures have been suggested. The experiments covered in this manual are easy to perform and have been used over the years in our laboratory for imparting practical training regularly to undergraduates and postgraduate students.Experiments were written in a step-wise manner for easy understanding.
Pancreatic cancer ranks as the fourth cause of cancer-associated deaths in the West. It is an agg... more Pancreatic cancer ranks as the fourth cause of cancer-associated deaths in the West. It is an aggressive cancer with poor prognosis and a huge mortality rate. Current treatment modalities used for treating PC (surgery and chemoradiotherapy) have limited outcomes due to stroma-induced chemoresistance. These shortcomings stress the need for new therapeutic solutions with increased efficacy and cytotoxicity for PC. In recent years, nanotechnology has emerged as a promising solution for improving diagnosis, drug delivery, and treatment in many cancers including PC. In this chapter, we explore the various nanotechnologies developed to date, their chemical composition, pharmacological properties, and activity. We also discuss their potential as drug delivery solutions for PC.
Colorectal cancer (CRC) is the most prevalent cancer around the world, and advancements in therap... more Colorectal cancer (CRC) is the most prevalent cancer around the world, and advancements in therapy continue to reduce illness and death. CRC examination and therapy with various nanotechnology-based methods have led to promising results, under scientific panel to establish novel therapeutic strategies. Today, the use of nanoparticles as a drug delivery has become the most auspicious form of cancer therapy. Many studies have revealed a keen benefit of phytochemicals and nanoparticles combined therapy for various cancers. In this chapter, we ensure a deeper discussion regarding the latest advancement of board nanoparticle drug-based phytochemical fabrication against colorectal cancer detection and treatment.
Cancer theranostics represents a strategy that aims at combining diagnosis with therapy through t... more Cancer theranostics represents a strategy that aims at combining diagnosis with therapy through the simultaneous imaging and targeted delivery of therapeutics to cancer cells. Recently, the folate receptor alpha has emerged as an attractive theranostic target due to its overexpression in multiple solid tumors and its great functional versatility. In fact, it can be incorporated into folate-conjugated nano-systems for imaging and drug delivery. Hence, it can be used along the line of personalized clinical strategies as both an imaging tool and a delivery method ensuring the selective transport of treatments to tumor cells, thus highlighting its theranostic qualities. In this review, we will explore these theranostic characteristics in detail and assess their clinical potential. We will also discuss the technological advances that have allowed the design of sophisticated folate-based nanocarriers harboring various chemical properties and suited for the transport of various therapeutic agents.
Female-specific cancers are the most common cancers in women worldwide. Early detection methods r... more Female-specific cancers are the most common cancers in women worldwide. Early detection methods remain unavailable for most of these cancers, signifying that most of them are diagnosed at later stages. Furthermore, current treatment options for most female-specific cancers are surgery, radiation and chemotherapy. Although important milestones in molecularly targeted approaches have been achieved lately, current therapeutic strategies for female-specific cancers remain limited, ineffective and plagued by the emergence of chemoresistance, which aggravates prognosis. Recently, the application of nanotechnology to the medical field has allowed the development of novel nano-based approaches for the management and treatment of cancers, including female-specific cancers. These approaches promise to improve patient survival rates by reducing side effects, enabling selective delivery of drugs to tumor tissues and enhancing the uptake of therapeutic compounds, thus increasing anti-tumor activity. In this review, we focus on the application of nano-based technologies to the design of novel and innovative diagnostic and therapeutic strategies in the context of female-specific cancers, highlighting their potential uses and limitations.
Aquaporins (AQPs) are hydrophobic integral trans-membrane channel proteins implicated in cellular... more Aquaporins (AQPs) are hydrophobic integral trans-membrane channel proteins implicated in cellular proliferation and water transport in various cancers. They compose a family of 13 different isoforms (25-34 kDa) in mammals that have been identified to date. These AQPs exhibit a unique pattern of tissue expression. Though they can be found in most tissues, they are mostly active in endothelial and epithelial tissues. In this review, the AQPs associated with female-specific cancers are comprehensively explored and their significant features are analysed. Recent findings revealing their role in female-specific carcinogenesis, especially cervical, ovarian, uterine/endometrial, and breast, will be also discussed.
The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune sy... more The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune system in healthy cells. However, it is upregulated in various malignancies, involved in cancer development and is also immunogenic. Furthermore, FRα is a tumor-associated antigen endowed with unique properties, thus rendering it a suitable target for immunotherapeutic development in cancer. Various anti- FRα immunotherapeutic strategies are thus currently being developed and clinically assessed for the treatment of various solid tumors. These approaches include passive anti-FRα immunotherapies, such as monoclonal antibodies, or active immunotherapies, such as CART, folate haptens and vaccines. In this review, we will explore the advances in the field of FRα-based immune therapies and discuss both their successes and shortcomings in the clinical setting.
The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune sy... more The glycoprotein FRα is a membrane-attached transport protein that is shielded from the immune system in healthy cells. However, it is upregulated in various malignancies, involved in cancer development and is also immunogenic. Furthermore, FRα is a tumor-associated antigen endowed with unique properties, thus rendering it a suitable target for immunotherapeutic development in cancer. Various anti- FRα immunotherapeutic strategies are thus currently being developed and clinically assessed for the treatment of various solid tumors. These approaches include passive anti-FRα immunotherapies, such as monoclonal antibodies, or active immunotherapies, such as CART, folate haptens and vaccines. In this review, we will explore the advances in the field of FRα-based immune therapies and discuss both their successes and shortcomings in the clinical setting.
The present study was carried out to isolation of yeast and production of ethanol using papaya ... more The present study was carried out to isolation of yeast and production of ethanol using papaya (Carica papaya) and grape (Vitis vinifera) pulps with isolated yeast. Using selective medium, the yeast strains were isolated from fruit pulps and differential tests were applied for identification of the yeast. Percentage of yield of ethanol and effect of different nutrient supplements for the production of ethanol was studied using isolated yeast as synthetic medium is control. The reducing sugar was also estimated after 72 hrs saccharification, the amount of reducing sugar increased significantly (14.6 g/100g) in papaya fruit than grape fruit pulp (10.3 g/ 100g) and it is evident that high amount of carbohydrate levels. The fermented papaya yielded (6.20%) more ethanol content than grape pulp (3.83%). Addition of carbohydrate supplement, sucrose and nitrogen supplement of soya meal produced high yield of ethanol with yeast in papaya (11.45% & 9.16 %) than grape pulp (7.61% & 6.03%). The present study revealed that the fully ripened papaya fruit is a good source for ethanol production and it is possibility of ethanol production from our locally available fruits using simple, cheap, and adaptive technology with biochemically characterized yeast isolated from fruit pulps
The present investigation was carried out to study the anti-bacterial activity of ethanolic
and m... more The present investigation was carried out to study the anti-bacterial activity of ethanolic and methanolic extracts of stem, berries and whole plant of Solanum nigrum. The antibacterial activity was detected against Bacillus subtilis, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Solanum nigrum plant extracts of, 10ìg, 50ìg and 100ìg were taken as per CLSI standards. The zones of inhibitions obtained were recorded and analyzed against standard control streptomycin. The methanolic extracts were showed highest antibacterial activity than ethanolic extracts. In both extracts, whole plant extract showed potential anti-bacterial activity than stem and berries. The results indicate that the whole plant extracts of Solanum nigrum may recommend to use in preparation of herbal drugs. ISSN:-2
Objective: Personal health practices have an important role in promotion of oral health of an ind... more Objective: Personal health practices have an important role in promotion of oral health of an individual. Dental diseases are irreversible, damage cannot be reverted. The knowledge among the people is of pivotal importance as these diseases cannot be identified at initial stages. Raising public awareness about dental check up may assist in early diagnosis. This study aims at finding out oral health knowledge and oral health behavior among Tirupati students. Methods: A structured Questionnaire was distributed to Post graduate students of Tirupati (N=190) during the academic year 2011-12. The respondents have filled in the forms in their class itself and ample time was given to respond. The questionnaire consisted information on general background, oral practices and oral health knowledge. Results: Oral health knowledge seemed to be very limited. More than half of the students didn’t visit a dentist for almost 2 years now. Only few knew the importance of fluorides in caries prevention. Very less proportion practiced dental flossing and used mouth washes. Only 10% of study group knew that a father could transmit S.mutans to the child. 68.42% knew that extraction is the not only possible treatment. Only 10.53% of the study group could attain a score of 5 but none could score the maximum points. Conclusion: It can be concluded that students had limited knowledge on oral hygiene and ideal oral hygiene practices. Their brushing practices are still far behind the international recommendation of twice a day. The establishment and strengthening of oral hygiene education, including the importance of tooth brushing for prevention of periodontal disease, has been proposed.
The present study was aimed to investigate the antibacterial activity of aqueous, methanolic and ... more The present study was aimed to investigate the antibacterial activity of aqueous, methanolic and ethanolic
extracts of root, fruit and whole plant of Cephalendra indica using disc diffusion method. The antibacterial
activity was detected against Gram-positive and Gram-negative bacteria, Bacillus subtilis, Bacillus ceresu,
Staphylococus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Different
solvent extracts of root, fruit and whole plant of Cephalendra indica was taken in 50,100,150 and 200 μg as
per CLSI standards. The zones of inhibitions obtained were recorded and analyzed against standard
controls, Streptomycin and Ampicillin. The methanolic extracts of fruit and whole plant were showed
highest antibacterial activity than ethanolic and aqueous extracts. The higher concentration of methanolic
extract of whole plant, maximum antibacterial activity was observed against to Gram-negative bacteria than
Gram-positive bacteria. When compared with fruit and root extracts of different solvents, methanolic
extract of whole plant exhibit high antibacterial activity. The results indicate that Cephalendra indica
whole plant also has potent antibacterial activity similar to fruit and leaves.
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Papers by Dr. Prameswari Kasa
and methanolic extracts of stem, berries and whole plant of Solanum nigrum. The
antibacterial activity was detected against Bacillus subtilis, Escherichia coli, Klebsiella
pneumonia and Pseudomonas aeruginosa. Solanum nigrum plant extracts of, 10ìg,
50ìg and 100ìg were taken as per CLSI standards. The zones of inhibitions obtained
were recorded and analyzed against standard control streptomycin. The methanolic
extracts were showed highest antibacterial activity than ethanolic extracts. In both
extracts, whole plant extract showed potential anti-bacterial activity than stem and
berries. The results indicate that the whole plant extracts of Solanum nigrum may
recommend to use in preparation of herbal drugs.
ISSN:-2
damage cannot be reverted. The knowledge among the people is of pivotal importance as these diseases cannot be identified at initial stages.
Raising public awareness about dental check up may assist in early diagnosis. This study aims at finding out oral health knowledge and oral
health behavior among Tirupati students. Methods: A structured Questionnaire was distributed to Post graduate students of Tirupati (N=190)
during the academic year 2011-12. The respondents have filled in the forms in their class itself and ample time was given to respond. The
questionnaire consisted information on general background, oral practices and oral health knowledge. Results: Oral health knowledge
seemed to be very limited. More than half of the students didn’t visit a dentist for almost 2 years now. Only few knew the importance of
fluorides in caries prevention. Very less proportion practiced dental flossing and used mouth washes. Only 10% of study group knew that a
father could transmit S.mutans to the child. 68.42% knew that extraction is the not only possible treatment. Only 10.53% of the study group
could attain a score of 5 but none could score the maximum points. Conclusion: It can be concluded that students had limited knowledge on
oral hygiene and ideal oral hygiene practices. Their brushing practices are still far behind the international recommendation of twice a day.
The establishment and strengthening of oral hygiene education, including the importance of tooth brushing for prevention of periodontal
disease, has been proposed.
extracts of root, fruit and whole plant of Cephalendra indica using disc diffusion method. The antibacterial
activity was detected against Gram-positive and Gram-negative bacteria, Bacillus subtilis, Bacillus ceresu,
Staphylococus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Different
solvent extracts of root, fruit and whole plant of Cephalendra indica was taken in 50,100,150 and 200 μg as
per CLSI standards. The zones of inhibitions obtained were recorded and analyzed against standard
controls, Streptomycin and Ampicillin. The methanolic extracts of fruit and whole plant were showed
highest antibacterial activity than ethanolic and aqueous extracts. The higher concentration of methanolic
extract of whole plant, maximum antibacterial activity was observed against to Gram-negative bacteria than
Gram-positive bacteria. When compared with fruit and root extracts of different solvents, methanolic
extract of whole plant exhibit high antibacterial activity. The results indicate that Cephalendra indica
whole plant also has potent antibacterial activity similar to fruit and leaves.
and methanolic extracts of stem, berries and whole plant of Solanum nigrum. The
antibacterial activity was detected against Bacillus subtilis, Escherichia coli, Klebsiella
pneumonia and Pseudomonas aeruginosa. Solanum nigrum plant extracts of, 10ìg,
50ìg and 100ìg were taken as per CLSI standards. The zones of inhibitions obtained
were recorded and analyzed against standard control streptomycin. The methanolic
extracts were showed highest antibacterial activity than ethanolic extracts. In both
extracts, whole plant extract showed potential anti-bacterial activity than stem and
berries. The results indicate that the whole plant extracts of Solanum nigrum may
recommend to use in preparation of herbal drugs.
ISSN:-2
damage cannot be reverted. The knowledge among the people is of pivotal importance as these diseases cannot be identified at initial stages.
Raising public awareness about dental check up may assist in early diagnosis. This study aims at finding out oral health knowledge and oral
health behavior among Tirupati students. Methods: A structured Questionnaire was distributed to Post graduate students of Tirupati (N=190)
during the academic year 2011-12. The respondents have filled in the forms in their class itself and ample time was given to respond. The
questionnaire consisted information on general background, oral practices and oral health knowledge. Results: Oral health knowledge
seemed to be very limited. More than half of the students didn’t visit a dentist for almost 2 years now. Only few knew the importance of
fluorides in caries prevention. Very less proportion practiced dental flossing and used mouth washes. Only 10% of study group knew that a
father could transmit S.mutans to the child. 68.42% knew that extraction is the not only possible treatment. Only 10.53% of the study group
could attain a score of 5 but none could score the maximum points. Conclusion: It can be concluded that students had limited knowledge on
oral hygiene and ideal oral hygiene practices. Their brushing practices are still far behind the international recommendation of twice a day.
The establishment and strengthening of oral hygiene education, including the importance of tooth brushing for prevention of periodontal
disease, has been proposed.
extracts of root, fruit and whole plant of Cephalendra indica using disc diffusion method. The antibacterial
activity was detected against Gram-positive and Gram-negative bacteria, Bacillus subtilis, Bacillus ceresu,
Staphylococus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Different
solvent extracts of root, fruit and whole plant of Cephalendra indica was taken in 50,100,150 and 200 μg as
per CLSI standards. The zones of inhibitions obtained were recorded and analyzed against standard
controls, Streptomycin and Ampicillin. The methanolic extracts of fruit and whole plant were showed
highest antibacterial activity than ethanolic and aqueous extracts. The higher concentration of methanolic
extract of whole plant, maximum antibacterial activity was observed against to Gram-negative bacteria than
Gram-positive bacteria. When compared with fruit and root extracts of different solvents, methanolic
extract of whole plant exhibit high antibacterial activity. The results indicate that Cephalendra indica
whole plant also has potent antibacterial activity similar to fruit and leaves.