Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria m... more Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mel-lonella) infected with the entomopathogenic nematodes:
Two gramineae species, Panicum maximum and Eleusine coracana, were exposed to salinity stress and... more Two gramineae species, Panicum maximum and Eleusine coracana, were exposed to salinity stress and leaf water status, solute concentrations in cell sap, levels of betaine aldehyde dehydrogenase (BADH) and BADH mRNA were measured. Panicum maximum was able to maintain a high turgor and high relative water content at low leaf water potentials, and this was associated with the greater capacity for osmotic adjustment. Major osmotica in Panicum maximum leaves were Na+, Cl-, sugar and glycinebetaine, and these solutes were increased by salinity. Na+ and glycinebetaine concentrations in Eleusine coracana leaves also were increased by salt stress, but these solutes were in significantly lower concentrations than those measured in Panicum maximum. BADH enzyme activity and BADH mRNA levels in Panicum maximum leaves were both increased by salt stress, and their expression coincided with the observed betaine accumulation. Although the addition of abscisic acid (ABA) to leaf disks of Panicum maxim...
Research Journal of Agriculture and Biological Sciences, 2009
... Ahmed M. El-Sharkawy, Sawsan S. Youssef, Reda EA Moghaieb, Eman Sobhy, 1 1 1 2 Rabab G. El Me... more ... Ahmed M. El-Sharkawy, Sawsan S. Youssef, Reda EA Moghaieb, Eman Sobhy, 1 1 1 2 Rabab G. El Mergawy and Etr HK Mohammed 1 1 ... Primer Total scorable band Polymorphic band Polymorphism % Band size range OPE A-10 11 3 27 280-2000 ...
In order to investigate the influence of genetic makeup on salt tolerance in wheat, seedlings of ... more In order to investigate the influence of genetic makeup on salt tolerance in wheat, seedlings of seven T. aestivum and two T. durum cultivars were subjected to salt stress for two weeks. Leaf osmotic adjustment, total sugars, free amino acids and proline under salt stress were studied. The concentration of total sugars, total free amino acids and free proline were increased by salinity. Salt stress decreased leaf osmotic potential in all cultivars. The difference in osmotic adjustment between the cultivars was correlated with the concentrations of minerals examined such as Na+ and K+. The salt tolerance in the T. aestivum cultivar Gemmeiza 10, and in T. durum cultivars Sohag and Beni Sweif is due to higher ability to maintain osmotic potential of the cells than the other cultivars by increases in osmoticum concentration under salt stress. The cultivars Gemmeiza10, Sohag and Beni Sweif showed higher peroxidase band intensity under salinity stress compared with the other cultivars use...
Ectoine is a common compatible solute in halophilic bacteria. Its biosynthesis originates from L-... more Ectoine is a common compatible solute in halophilic bacteria. Its biosynthesis originates from L-aspartate β-semialdehyde and requires three enzymes: L-2, 4-diaminobutyric acid aminotransferase (gene: ect B), L-2,4-diaminobutyric acid acetyl transferase (gene: ect A) and L-ectoine synthase (gene: ect C). Genetically engineered tomato plants expressing the three H. elongata genes (ectA, ectB, and ectC) generated showed no phenotypic abnormality. Expression of the ectoine biosynthetic genes was detected in the T3 transgenic plants by Northern blot analysis. The ectoine accumulating T3 plants were evaluated for salt tolerance by examining their photosynthestic activity, osmotic adjustment and carbon partitioning. Nuclear magnetic resonance (NMR) detected the accumulation of ectoine. The concentration of ectoine increased with increasing salinity. The transgenic lines showed higher activities of peroxidase, while the malondialdehyde (MDA) concentration was decreased under salinity stres...
To study the influence of genetic background on the transformation and regeneration of cultivated... more To study the influence of genetic background on the transformation and regeneration of cultivated tomato plants, hairy root lines of tomato (Lycopersicon esculentum) were obtained by inoculating the hypocotyl explants of three tomato cultivars with the Agrobacterium rhizogenes strain DCAR-2, which harbors the pBI-121 binary vector. The Ri-T-DNA transformation into the plant DNA was confirmed by both of mikimopine and GUS assay analyses. The regeneration efficiency from hairy root explants was assessed. The data indicated that white embryonic calli were formed within two weeks in the presence of 2 mgl(-1) 2, 4-D plus 0.25 mgl(-1) kinetin. Adventitious shoots emerged from the embryonic callus in the presence of 1 mgl(-1) GA3 along with 0.5 mgl(-1) NAA. The regeneration frequency was higher in the cultivar UC-97, followed by Momotaro and then Edkawi. Molecular confirmation of the integration of the GUS gene into the hairy root-derived plants genomes was done via PCR using GUS-specific ...
Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria m... more Five bacterial strains were isolated from the hemocoel of the greater wax moth larvae (Galleria mel-lonella) infected with the entomopathogenic nematodes:
Two gramineae species, Panicum maximum and Eleusine coracana, were exposed to salinity stress and... more Two gramineae species, Panicum maximum and Eleusine coracana, were exposed to salinity stress and leaf water status, solute concentrations in cell sap, levels of betaine aldehyde dehydrogenase (BADH) and BADH mRNA were measured. Panicum maximum was able to maintain a high turgor and high relative water content at low leaf water potentials, and this was associated with the greater capacity for osmotic adjustment. Major osmotica in Panicum maximum leaves were Na+, Cl-, sugar and glycinebetaine, and these solutes were increased by salinity. Na+ and glycinebetaine concentrations in Eleusine coracana leaves also were increased by salt stress, but these solutes were in significantly lower concentrations than those measured in Panicum maximum. BADH enzyme activity and BADH mRNA levels in Panicum maximum leaves were both increased by salt stress, and their expression coincided with the observed betaine accumulation. Although the addition of abscisic acid (ABA) to leaf disks of Panicum maxim...
Research Journal of Agriculture and Biological Sciences, 2009
... Ahmed M. El-Sharkawy, Sawsan S. Youssef, Reda EA Moghaieb, Eman Sobhy, 1 1 1 2 Rabab G. El Me... more ... Ahmed M. El-Sharkawy, Sawsan S. Youssef, Reda EA Moghaieb, Eman Sobhy, 1 1 1 2 Rabab G. El Mergawy and Etr HK Mohammed 1 1 ... Primer Total scorable band Polymorphic band Polymorphism % Band size range OPE A-10 11 3 27 280-2000 ...
In order to investigate the influence of genetic makeup on salt tolerance in wheat, seedlings of ... more In order to investigate the influence of genetic makeup on salt tolerance in wheat, seedlings of seven T. aestivum and two T. durum cultivars were subjected to salt stress for two weeks. Leaf osmotic adjustment, total sugars, free amino acids and proline under salt stress were studied. The concentration of total sugars, total free amino acids and free proline were increased by salinity. Salt stress decreased leaf osmotic potential in all cultivars. The difference in osmotic adjustment between the cultivars was correlated with the concentrations of minerals examined such as Na+ and K+. The salt tolerance in the T. aestivum cultivar Gemmeiza 10, and in T. durum cultivars Sohag and Beni Sweif is due to higher ability to maintain osmotic potential of the cells than the other cultivars by increases in osmoticum concentration under salt stress. The cultivars Gemmeiza10, Sohag and Beni Sweif showed higher peroxidase band intensity under salinity stress compared with the other cultivars use...
Ectoine is a common compatible solute in halophilic bacteria. Its biosynthesis originates from L-... more Ectoine is a common compatible solute in halophilic bacteria. Its biosynthesis originates from L-aspartate β-semialdehyde and requires three enzymes: L-2, 4-diaminobutyric acid aminotransferase (gene: ect B), L-2,4-diaminobutyric acid acetyl transferase (gene: ect A) and L-ectoine synthase (gene: ect C). Genetically engineered tomato plants expressing the three H. elongata genes (ectA, ectB, and ectC) generated showed no phenotypic abnormality. Expression of the ectoine biosynthetic genes was detected in the T3 transgenic plants by Northern blot analysis. The ectoine accumulating T3 plants were evaluated for salt tolerance by examining their photosynthestic activity, osmotic adjustment and carbon partitioning. Nuclear magnetic resonance (NMR) detected the accumulation of ectoine. The concentration of ectoine increased with increasing salinity. The transgenic lines showed higher activities of peroxidase, while the malondialdehyde (MDA) concentration was decreased under salinity stres...
To study the influence of genetic background on the transformation and regeneration of cultivated... more To study the influence of genetic background on the transformation and regeneration of cultivated tomato plants, hairy root lines of tomato (Lycopersicon esculentum) were obtained by inoculating the hypocotyl explants of three tomato cultivars with the Agrobacterium rhizogenes strain DCAR-2, which harbors the pBI-121 binary vector. The Ri-T-DNA transformation into the plant DNA was confirmed by both of mikimopine and GUS assay analyses. The regeneration efficiency from hairy root explants was assessed. The data indicated that white embryonic calli were formed within two weeks in the presence of 2 mgl(-1) 2, 4-D plus 0.25 mgl(-1) kinetin. Adventitious shoots emerged from the embryonic callus in the presence of 1 mgl(-1) GA3 along with 0.5 mgl(-1) NAA. The regeneration frequency was higher in the cultivar UC-97, followed by Momotaro and then Edkawi. Molecular confirmation of the integration of the GUS gene into the hairy root-derived plants genomes was done via PCR using GUS-specific ...
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