The characterization of an optical absorption based oneshot sensor is described for the determina... more The characterization of an optical absorption based oneshot sensor is described for the determination of thiourea based on the immobilization of new nanocomposite of MWCNT-graft-poly(citric acid)/Au on a triacetylcellulose membrane. MWCNT-g-PCA/Au is covalently bonded to a transparent triacetylcellulose film. Thiourea reacts with the immobilized nanocomposite and causes a decrease in the absorbance of the film at 300 nm. The response time of sensing phase is within 5 min depending on the concentration of thiourea. This sensing phase has a linear range of 40-250 and 250-1000 mgL −1 for thiourea with a limit of detection of 11 mgL −1 at a wavelength of 300 nm. The nanosensor was successfully applied for the determination of thiourea in spring water and orange peel.
Abstract The present research generally relates to an assay for determining the presence of an an... more Abstract The present research generally relates to an assay for determining the presence of an analyte, and more particularly to a new approach toward the development of higher sensitive immunoassay-based dipsticks which make use of bio-functionalized nano‑gold as a detection reagent, and even more particularly to utilizing CNT-g-PCA-Au for labeling antibodies in order to improve the sensitivity of the immunoassay-based strip test.
SUMMARY The causal agent of mild chlorosis and stunting of sugar beet in Iran was identified as C... more SUMMARY The causal agent of mild chlorosis and stunting of sugar beet in Iran was identified as Chickpea chlorotic dwarf virus (CpCDV). The virus reacted positively with CpCDV-specific antibodies in ELISA, but not with antibodies specific to other viruses of the family Geminiviridae (Beet curly top virus, Bean golden mosaic virus and Tomato yellow leaf curl virus). The virus isolate under study (SK-11) was transmitted by grafting and by the leafhopper Orosius orientalis Matsumara to a range of plant species in the Chenopodiaceae, Fabaceae and Solanaceae, and induced symptoms like those reported for CpCDV. By using a pair of degenerate primers, a short viral genome fragment was amplified by PCR, cloned and sequenced, revealing a close homology to Bean yellow dwarf virus (84.9%-85.3% identity), and a more distant relationship to Tobacco yellow dwarf virus (58.8%). In the present study, two specific primers were designed for detecting CpCDV which allowed the amplification of a DNA frag...
The main Iranian sugar beet (Beta vulgaris) growing areas were surveyed for the occurrence and in... more The main Iranian sugar beet (Beta vulgaris) growing areas were surveyed for the occurrence and incidence of Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus (BSBV) and Beet virus Q (BVQ). Root samples from 3,486 plants were collected from 184 commercial sugar beet fields of 14 provinces from randomly selected and symptomatic plants and analysed by tissue-blot immunoassay (TBIA). Serological diagnoses were confirmed by reverse transcription-polymerase chain reaction tests (RT-PCR) using specific primers. Based on TBIA data, BNYVV was prevalent (overall incidence of 52.4%), followed by BSBV (9.8%) and BVQ (1.4%). High levels of virus infections (more than 70%) were found in Ardabil, Esfahan, Fars, Khorasan and Zanjan provinces, whereas Khuzestan was apparently “virus-free”. The putative vector of the soil-borne viruses, Polymyxa betae, was also detected by RT-PCR in 179 fields surveyed.
In this study, the full-length nucleotide sequences of four Iranian PVY isolates belonging to PVY... more In this study, the full-length nucleotide sequences of four Iranian PVY isolates belonging to PVY(N) strain were determined. The genome of Iranian PVY isolates were 9,703-9,707 nucleotides long encoding all potyviral cistrons including P1, HC-Pro, P3, 6K1, CI, 6K2, VPg, NIa-Pro, NIb and CP with coding regions of 825, 1,395, 1,095, 156, 1,902, 156, 564, 732, 1,557 and 801 nucleotides in length, respectively. The length of pipo, embedded in the P3 cistron, was 231 nucleotides. Phylogenetic analysis showed that the Iranian isolates clustered with European recombinant NTN isolates in the N lineage. Recombination analysis demonstrated that Iranian PVY(N) isolates had a typical European PVY(NTN) genome having three recombinant junctions while PVY(N) and PVY(O) were identified as the parents. We used dN/dS methods to detect candidate amino acid positions for positive selection in viral proteins. The mean ω ratio differed among different genes. Using model M0, ω values were 0.267 (P1), 0.08...
In this study, vascular stents were fabricated from poly (lactide-ɛ-caprolactone)/collagen/nano-h... more In this study, vascular stents were fabricated from poly (lactide-ɛ-caprolactone)/collagen/nano-hydroxyapatite (PLCL/Col/nHA) by electrospinning, and the surface morphology and breaking strength were observed or measured through scanning electron microscopy and tensile tests. The anti-clotting properties of stents were evaluated for anticoagulation surfaces modified by the electrostatic layer-by-layer self-assembly technique. In addition, nano-composite scaffolds of poly (lactic-co-glycolic acid)/polycaprolactone/nano-hydroxyapatite (PLGA/PCL/nHA) loaded with the vascular stents were prepared by thermoforming-particle leaching and their basic performance and osteogenesis were tested in vitro and in vivo. The results show that the PLCL/Col/nHA stents and PLGA/PCL/nHA nano-composite scaffolds had good surface structures, mechanical properties, biocompatibility and could guide bone regeneration. These may provide a new way to build vascularized-tissue engineered bone to repair large bo...
Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields ... more Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields of Firouzkoh Province, Iran, during the summer of 1998. Infected plants died before the end of the growing season. Necrosis was more severe in cv. Agria than in cvs. Ajaxs and Arinda. A high population of Thrips tabaci was observed in August and September. Tomato spotted wilt virus (TSWV) (1) was detected in affected potatoes by using specific TSWV-IgG (from Bioreba) in double-antibody sandwich enzyme linked immunosorbent assay and by indicator plant reactions. Mechanical inoculation of indicator plants with leaf extracts of symptomatic potatoes produce necrotic local lesions in Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Vicia faba, Vigna sinensis, Phaseolus aureus var. Gohar, P. vulgaris, and Petunia hybrida. The virus caused systemic necrosis in Capsicum frutescens, Datura stramonium, D. metel, Nicotiana glutinosa, N. rustica, and Trapaeolum majus, preceded by systemic ch...
The characterization of an optical absorption based oneshot sensor is described for the determina... more The characterization of an optical absorption based oneshot sensor is described for the determination of thiourea based on the immobilization of new nanocomposite of MWCNT-graft-poly(citric acid)/Au on a triacetylcellulose membrane. MWCNT-g-PCA/Au is covalently bonded to a transparent triacetylcellulose film. Thiourea reacts with the immobilized nanocomposite and causes a decrease in the absorbance of the film at 300 nm. The response time of sensing phase is within 5 min depending on the concentration of thiourea. This sensing phase has a linear range of 40-250 and 250-1000 mgL −1 for thiourea with a limit of detection of 11 mgL −1 at a wavelength of 300 nm. The nanosensor was successfully applied for the determination of thiourea in spring water and orange peel.
Abstract The present research generally relates to an assay for determining the presence of an an... more Abstract The present research generally relates to an assay for determining the presence of an analyte, and more particularly to a new approach toward the development of higher sensitive immunoassay-based dipsticks which make use of bio-functionalized nano‑gold as a detection reagent, and even more particularly to utilizing CNT-g-PCA-Au for labeling antibodies in order to improve the sensitivity of the immunoassay-based strip test.
SUMMARY The causal agent of mild chlorosis and stunting of sugar beet in Iran was identified as C... more SUMMARY The causal agent of mild chlorosis and stunting of sugar beet in Iran was identified as Chickpea chlorotic dwarf virus (CpCDV). The virus reacted positively with CpCDV-specific antibodies in ELISA, but not with antibodies specific to other viruses of the family Geminiviridae (Beet curly top virus, Bean golden mosaic virus and Tomato yellow leaf curl virus). The virus isolate under study (SK-11) was transmitted by grafting and by the leafhopper Orosius orientalis Matsumara to a range of plant species in the Chenopodiaceae, Fabaceae and Solanaceae, and induced symptoms like those reported for CpCDV. By using a pair of degenerate primers, a short viral genome fragment was amplified by PCR, cloned and sequenced, revealing a close homology to Bean yellow dwarf virus (84.9%-85.3% identity), and a more distant relationship to Tobacco yellow dwarf virus (58.8%). In the present study, two specific primers were designed for detecting CpCDV which allowed the amplification of a DNA frag...
The main Iranian sugar beet (Beta vulgaris) growing areas were surveyed for the occurrence and in... more The main Iranian sugar beet (Beta vulgaris) growing areas were surveyed for the occurrence and incidence of Beet necrotic yellow vein virus (BNYVV), Beet soil-borne virus (BSBV) and Beet virus Q (BVQ). Root samples from 3,486 plants were collected from 184 commercial sugar beet fields of 14 provinces from randomly selected and symptomatic plants and analysed by tissue-blot immunoassay (TBIA). Serological diagnoses were confirmed by reverse transcription-polymerase chain reaction tests (RT-PCR) using specific primers. Based on TBIA data, BNYVV was prevalent (overall incidence of 52.4%), followed by BSBV (9.8%) and BVQ (1.4%). High levels of virus infections (more than 70%) were found in Ardabil, Esfahan, Fars, Khorasan and Zanjan provinces, whereas Khuzestan was apparently “virus-free”. The putative vector of the soil-borne viruses, Polymyxa betae, was also detected by RT-PCR in 179 fields surveyed.
In this study, the full-length nucleotide sequences of four Iranian PVY isolates belonging to PVY... more In this study, the full-length nucleotide sequences of four Iranian PVY isolates belonging to PVY(N) strain were determined. The genome of Iranian PVY isolates were 9,703-9,707 nucleotides long encoding all potyviral cistrons including P1, HC-Pro, P3, 6K1, CI, 6K2, VPg, NIa-Pro, NIb and CP with coding regions of 825, 1,395, 1,095, 156, 1,902, 156, 564, 732, 1,557 and 801 nucleotides in length, respectively. The length of pipo, embedded in the P3 cistron, was 231 nucleotides. Phylogenetic analysis showed that the Iranian isolates clustered with European recombinant NTN isolates in the N lineage. Recombination analysis demonstrated that Iranian PVY(N) isolates had a typical European PVY(NTN) genome having three recombinant junctions while PVY(N) and PVY(O) were identified as the parents. We used dN/dS methods to detect candidate amino acid positions for positive selection in viral proteins. The mean ω ratio differed among different genes. Using model M0, ω values were 0.267 (P1), 0.08...
In this study, vascular stents were fabricated from poly (lactide-ɛ-caprolactone)/collagen/nano-h... more In this study, vascular stents were fabricated from poly (lactide-ɛ-caprolactone)/collagen/nano-hydroxyapatite (PLCL/Col/nHA) by electrospinning, and the surface morphology and breaking strength were observed or measured through scanning electron microscopy and tensile tests. The anti-clotting properties of stents were evaluated for anticoagulation surfaces modified by the electrostatic layer-by-layer self-assembly technique. In addition, nano-composite scaffolds of poly (lactic-co-glycolic acid)/polycaprolactone/nano-hydroxyapatite (PLGA/PCL/nHA) loaded with the vascular stents were prepared by thermoforming-particle leaching and their basic performance and osteogenesis were tested in vitro and in vivo. The results show that the PLCL/Col/nHA stents and PLGA/PCL/nHA nano-composite scaffolds had good surface structures, mechanical properties, biocompatibility and could guide bone regeneration. These may provide a new way to build vascularized-tissue engineered bone to repair large bo...
Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields ... more Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields of Firouzkoh Province, Iran, during the summer of 1998. Infected plants died before the end of the growing season. Necrosis was more severe in cv. Agria than in cvs. Ajaxs and Arinda. A high population of Thrips tabaci was observed in August and September. Tomato spotted wilt virus (TSWV) (1) was detected in affected potatoes by using specific TSWV-IgG (from Bioreba) in double-antibody sandwich enzyme linked immunosorbent assay and by indicator plant reactions. Mechanical inoculation of indicator plants with leaf extracts of symptomatic potatoes produce necrotic local lesions in Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Vicia faba, Vigna sinensis, Phaseolus aureus var. Gohar, P. vulgaris, and Petunia hybrida. The virus caused systemic necrosis in Capsicum frutescens, Datura stramonium, D. metel, Nicotiana glutinosa, N. rustica, and Trapaeolum majus, preceded by systemic ch...
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