Ovarian carcinomas that have a distinctive natural history with early dissemination are particularly problematic. The aim of this immunohistochemical study was to assess whether the nm23-H1 gene product, which in some tumors shows inverse... more
Ovarian carcinomas that have a distinctive natural history with early dissemination are particularly problematic. The aim of this immunohistochemical study was to assess whether the nm23-H1 gene product, which in some tumors shows inverse association with metastatic potential, could serve as a prognostic marker for ovarian carcinomas. The study, based on 73 benign and 54 malignant ovarian tumors, showed clear differences in the frequency of nm23-H1-positive samples, the intensity of staining and the histological localization of this protein. Differences were observed between normal ovary samples and benign lesions as well as between benign tumors and ovarian carcinomas and were highly significant. Furthermore, carcinomas that had detectable metastasis at the time of surgery were negative for nm23-H1 protein more frequently than those that did not. Although this is a prospective study in which collection of clinical data is ongoing, the results strongly suggest that nm23-H1 may serve...
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The accuracy of combined use of serum CA 125 and transvaginal color Doppler for preoperative assessment of ovarian lesions in premenopausal patients was evaluated. Seventy-six ovarian lesions were analyzed the day before surgery using... more
The accuracy of combined use of serum CA 125 and transvaginal color Doppler for preoperative assessment of ovarian lesions in premenopausal patients was evaluated. Seventy-six ovarian lesions were analyzed the day before surgery using transvaginal color Doppler ultrasound. On the same day, serum CA 125 was measured in each patient. A novel index is proposed for the detection of ovarian malignancy combining resistance index (RI) obtained from newly formed vessels within the ovarian lesion and serum CA 125 level: (RI/CA 125) x 100. Values below the cut-off value of 1.5 were associated with a high risk of ovarian malignancy. Assessment of ovarian lesions by the novel index had a sensitivity and specificity of 94.44% and 100%, while positive and negative predictive values were 100% and 98.31%, respectively. The best prediction of ovarian malignancy was achieved by a combined use of transvaginal color Doppler ultrasound and serum CA 125 in the form of index: (RI/CA 125) x 100.
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... Patients The present study included 30 married couples enrolled in a pro-gramme for assisted reproduction and 23 patients who presented for prenatal diagnosis at Sveti Duh Hospital ... Cytogenetic analysis showed normal karyotypes,... more
... Patients The present study included 30 married couples enrolled in a pro-gramme for assisted reproduction and 23 patients who presented for prenatal diagnosis at Sveti Duh Hospital ... Cytogenetic analysis showed normal karyotypes, nine female (46, XX) and six male (46, XY). ...
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The objective was to compare the results of a complete chromosomal, genetic and histological investigation in 13 azoospermic men with the results of the intracytoplasmic sperm injection (ICSI) procedure. Peripheral blood samples were used... more
The objective was to compare the results of a complete chromosomal, genetic and histological investigation in 13 azoospermic men with the results of the intracytoplasmic sperm injection (ICSI) procedure. Peripheral blood samples were used for the measurement of follicle-stimulating hormone (FSH) levels, chromosomal analysis, microdeletions in the azoospermia factor (AZF) region of the Y chromosome and cystic fibrosis transmembrane conductance regulator (CFTR) mutation analysis. Testicular tissue was used for histological scoring and cytogenetic evaluation. Peripheral blood cytogenetic analysis revealed a normal male karyotype in all cases. Chromosomal analysis from testicular tissue revealed a mosaicism for the terminal deletion of chromosome 22 with a breakpoint site at 22q13 in one patient with congenital bilateral absence of the vas deferens (CBAVD). Deletions in the AZFa, ATFb, and AZFc regions were not detected. The CFTR mutational analysis showed normal results in all patients. Cytogenetic evaluation of testicular tissue should be performed in non-obstructive and obstructive azoospermic patients as well as in patients with multiple failed IVF and recurrent spontaneous abortion.