Zanthoxylum acanthopodium has several biological activities, such as antimicrobial, anti-inflamma... more Zanthoxylum acanthopodium has several biological activities, such as antimicrobial, anti-inflammatory, and antioxidant properties. This strong antioxidant herb can be used as a drug for hypertension. FasL and cytochrome c expression play roles in the apoptotic pathway in the placenta. This study focused on the histological change in apoptosis via cytochrome c and Fas ligand expression by investigating whether Zanthoxylum acanthopodium (ZA) fruits affect apoptosis. The present study consisted of five treatments: Normal pregnant rats (C), Hypertension rats (C +), hypertension rats + extra virgin olive oil (EVOO) (T1), Hypertension rats + ZA (T2), and hypertension rats + EVOO + ZA (T3). Hypertension was induced in rats by injecting 3 mlml of 6% NaCl. Nanoherbal of ZA (100 mg/kg BW) and EVOO (1 ml) were given on the 13th-19th days of pregnancy. Pregnant rats were dissected on the 20th day of pregnancy by cervical dislocation. ELISA assays were performed for the analysis of HSP-70 expression. Immunohistochemistry and TUNEL assays were used to analyse the histological changes in placental tissue. The results showed that cytochrome c and FasL protein exposure levels in the labyrinth, basal, and yolk sac zones were increased during hypertensive pregnancy (P < 0.0001) in rats. The administration of nanoherbal of ZA decreased the expression of cytochrome c and FasL. A significant difference was found in the combination of nanoherbal of ZA and EVOO.
Journal of Pharmacy & Pharmacognosy Research, 2021
Context: Gallic acid and quercetin in sikkam leaves (Bischofia javanica) have scientific potentia... more Context: Gallic acid and quercetin in sikkam leaves (Bischofia javanica) have scientific potential as antidiabetic agents. Quercetin suppresses hyperglycemia by inhibiting active glucose transport. Meanwhile, gallic acid acts as an antidiabetic is closely related to its antioxidant properties. Antioxidant compounds neutralize cells that experience oxidative stress by donating their hydrogen atoms.
Aims: To analyze the degree of insulitis and insulin expression in the pancreatic cells of rats induced diabetes mellitus (DM) after giving B. javanica leaves extract.
Methods: The treatment groups consisted of G0: negative control (-); G1: positive control (alloxan induction + standard feed); G2: alloxan induction + 300 mg/kg BW of B. javanica leaves ethanol extract; G3: alloxan induction + 600 mg/kg BW of B. javanica leaves ethanol extract; G4: alloxan induction + ethanol extract of B. javanica leaves 900 mg/kg BW and G5: alloxan induction + glibenclamide 4.5 mg/kg BW. At day 28, the rats were sacrificed, and the pancreatic tissue dissected. This was analyzed for degree of insulitis and insulin expression by anti-insulin antibodies using immunohistochemistry and hematoxylin-eosin.
Results: There was a significant difference (p = 0.000) in insulin expression and insulitis degree. By the increase of B. javanica leaves dose, the insulin expression value also increased, and the degree of insulitis in Langerhans’ islets of DM rats was decreased. Islets of Langerhans in insulin production returned to normal after being given B. javanica ethanol extract 900 mg/kg BW like glibenclamide.
Conclusions: Bischofia javanica ethanol extract increased insulin production and reduced the degree of insulitis in the islets of Langerhans histology. Further research is needed to determine the extent to which this extract protects from the sequelae of diabetes.
Zanthoxylum acanthopodium has several biological activities, such as antimicrobial, anti-inflamma... more Zanthoxylum acanthopodium has several biological activities, such as antimicrobial, anti-inflammatory, and antioxidant properties. This strong antioxidant herb can be used as a drug for hypertension. FasL and cytochrome c expression play roles in the apoptotic pathway in the placenta. This study focused on the histological change in apoptosis via cytochrome c and Fas ligand expression by investigating whether Zanthoxylum acanthopodium (ZA) fruits affect apoptosis. The present study consisted of five treatments: Normal pregnant rats (C), Hypertension rats (C +), hypertension rats + extra virgin olive oil (EVOO) (T1), Hypertension rats + ZA (T2), and hypertension rats + EVOO + ZA (T3). Hypertension was induced in rats by injecting 3 mlml of 6% NaCl. Nanoherbal of ZA (100 mg/kg BW) and EVOO (1 ml) were given on the 13th-19th days of pregnancy. Pregnant rats were dissected on the 20th day of pregnancy by cervical dislocation. ELISA assays were performed for the analysis of HSP-70 expression. Immunohistochemistry and TUNEL assays were used to analyse the histological changes in placental tissue. The results showed that cytochrome c and FasL protein exposure levels in the labyrinth, basal, and yolk sac zones were increased during hypertensive pregnancy (P < 0.0001) in rats. The administration of nanoherbal of ZA decreased the expression of cytochrome c and FasL. A significant difference was found in the combination of nanoherbal of ZA and EVOO.
Journal of Pharmacy & Pharmacognosy Research, 2021
Context: Gallic acid and quercetin in sikkam leaves (Bischofia javanica) have scientific potentia... more Context: Gallic acid and quercetin in sikkam leaves (Bischofia javanica) have scientific potential as antidiabetic agents. Quercetin suppresses hyperglycemia by inhibiting active glucose transport. Meanwhile, gallic acid acts as an antidiabetic is closely related to its antioxidant properties. Antioxidant compounds neutralize cells that experience oxidative stress by donating their hydrogen atoms.
Aims: To analyze the degree of insulitis and insulin expression in the pancreatic cells of rats induced diabetes mellitus (DM) after giving B. javanica leaves extract.
Methods: The treatment groups consisted of G0: negative control (-); G1: positive control (alloxan induction + standard feed); G2: alloxan induction + 300 mg/kg BW of B. javanica leaves ethanol extract; G3: alloxan induction + 600 mg/kg BW of B. javanica leaves ethanol extract; G4: alloxan induction + ethanol extract of B. javanica leaves 900 mg/kg BW and G5: alloxan induction + glibenclamide 4.5 mg/kg BW. At day 28, the rats were sacrificed, and the pancreatic tissue dissected. This was analyzed for degree of insulitis and insulin expression by anti-insulin antibodies using immunohistochemistry and hematoxylin-eosin.
Results: There was a significant difference (p = 0.000) in insulin expression and insulitis degree. By the increase of B. javanica leaves dose, the insulin expression value also increased, and the degree of insulitis in Langerhans’ islets of DM rats was decreased. Islets of Langerhans in insulin production returned to normal after being given B. javanica ethanol extract 900 mg/kg BW like glibenclamide.
Conclusions: Bischofia javanica ethanol extract increased insulin production and reduced the degree of insulitis in the islets of Langerhans histology. Further research is needed to determine the extent to which this extract protects from the sequelae of diabetes.
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Papers by Salomo Hutahaean
Aims: To analyze the degree of insulitis and insulin expression in the pancreatic cells of rats induced diabetes mellitus (DM) after giving B. javanica leaves extract.
Methods: The treatment groups consisted of G0: negative control (-); G1: positive control (alloxan induction + standard feed); G2: alloxan induction + 300 mg/kg BW of B. javanica leaves ethanol extract; G3: alloxan induction + 600 mg/kg BW of B. javanica leaves ethanol extract; G4: alloxan induction + ethanol extract of B. javanica leaves 900 mg/kg BW and G5: alloxan induction + glibenclamide 4.5 mg/kg BW. At day 28, the rats were sacrificed, and the pancreatic tissue dissected. This was analyzed for degree of insulitis and insulin expression by anti-insulin antibodies using immunohistochemistry and hematoxylin-eosin.
Results: There was a significant difference (p = 0.000) in insulin expression and insulitis degree. By the increase of B. javanica leaves dose, the insulin expression value also increased, and the degree of insulitis in Langerhans’ islets of DM rats was decreased. Islets of Langerhans in insulin production returned to normal after being given B. javanica ethanol extract 900 mg/kg BW like glibenclamide.
Conclusions: Bischofia javanica ethanol extract increased insulin production and reduced the degree of insulitis in the islets of Langerhans histology. Further research is needed to determine the extent to which this extract protects from the sequelae of diabetes.
Aims: To analyze the degree of insulitis and insulin expression in the pancreatic cells of rats induced diabetes mellitus (DM) after giving B. javanica leaves extract.
Methods: The treatment groups consisted of G0: negative control (-); G1: positive control (alloxan induction + standard feed); G2: alloxan induction + 300 mg/kg BW of B. javanica leaves ethanol extract; G3: alloxan induction + 600 mg/kg BW of B. javanica leaves ethanol extract; G4: alloxan induction + ethanol extract of B. javanica leaves 900 mg/kg BW and G5: alloxan induction + glibenclamide 4.5 mg/kg BW. At day 28, the rats were sacrificed, and the pancreatic tissue dissected. This was analyzed for degree of insulitis and insulin expression by anti-insulin antibodies using immunohistochemistry and hematoxylin-eosin.
Results: There was a significant difference (p = 0.000) in insulin expression and insulitis degree. By the increase of B. javanica leaves dose, the insulin expression value also increased, and the degree of insulitis in Langerhans’ islets of DM rats was decreased. Islets of Langerhans in insulin production returned to normal after being given B. javanica ethanol extract 900 mg/kg BW like glibenclamide.
Conclusions: Bischofia javanica ethanol extract increased insulin production and reduced the degree of insulitis in the islets of Langerhans histology. Further research is needed to determine the extent to which this extract protects from the sequelae of diabetes.