Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2]... more Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2] (1), [Hg(2,2′-bipy)2(SCN)2] (2) and [Hg(phen)2(NO3)2] (3) have been synthesized and characterized by elemental analysis, IR, H NMR and C NMR spectroscopy. The thermal stability of 1–3 were studied by thermal gravimetric (TG) and differential thermal analyses (DTA). The structure of 1 has been confirmed by X-ray crystallography. The complex is a
Frozen sections of lymph nodes from normal mice were examined by immunofluorescence with anti-T l... more Frozen sections of lymph nodes from normal mice were examined by immunofluorescence with anti-T lymphocyte and anti-Ig reagents and by autoradiography following [3H]TdR administration. The scattered T lymphocytes present among B lymphocytes of primary follicles were found to be mostly nonproliferating; the few proliferating cells in primary follicles are therefore predominantly B lymphocytes. More than one-half (56%) of these proliferating B lymphocytes were found to be in direct contact with a T lymphocyte; this incidence is 5-fold higher than that expected by random association. In germinal centers, virtually all proliferating cells were found to be non-T lymphocytes as well. The association of proliferating B cells with T cells may be the result of specific cooperation between these two cell types. These data are discussed in the context of what is known of T/B collaboration and of primary follicles as a possible site of this interaction.
Bacillus Calmette-Guerin (BCG) is the only available vaccine against tuberculosis. The research f... more Bacillus Calmette-Guerin (BCG) is the only available vaccine against tuberculosis. The research for an improved vaccine is currently a very active field of investigation. In the present study, adjuvanticity effect of sterile sodium alginate on subcutaneous BCG vaccination in BALB/c mice was investigated. Mice were vaccinated subcutaneously with BCG plus alginate and the immune response and protective effect were compared to those of mice vaccinated with BCG alone by the same route. Proliferative and delayed-type hypersensitivity responses, IFN-gamma, specific anti-mycobacterium total IgG, IgG1 and IgG2a production were significantly higher in mice immunized subcutaneously with BCG plus alginate in comparison with results of mice immunized with BCG alone. Following systemic infection with BCG, mice vaccinated with BCG plus alginate had lower mean bacterial count compared to those vaccinated with BCG alone. The immune responses induced by subcutaneous administration of BCG plus alginate were significantly better than the responses induced by standard BCG vaccination.
Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2]... more Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2] (1), [Hg(2,2′-bipy)2(SCN)2] (2) and [Hg(phen)2(NO3)2] (3) have been synthesized and characterized by elemental analysis, IR, H NMR and C NMR spectroscopy. The thermal stability of 1–3 were studied by thermal gravimetric (TG) and differential thermal analyses (DTA). The structure of 1 has been confirmed by X-ray crystallography. The complex is a
Background: Different methods have been used for BCG vaccination. Alginate microspheres are usefu... more Background: Different methods have been used for BCG vaccination. Alginate microspheres are useful in delivery of vaccines to the gastrointestinal tract by oral route. Objective: To compare the immune response following oral microencapsulated and ...
Autoimmune type 1 diabetes mellitus is caused by the T-cell mediated immune destruction of the in... more Autoimmune type 1 diabetes mellitus is caused by the T-cell mediated immune destruction of the insulin-producing beta-cell in pancreatic islets of Langerhans. The specificity of the auto-antibodies and of the auto-reactive T cells has been investigated, in which several auto-antigens were proposed. The purpose of this study was to determine whether glutamic acid decarboxylase (GAD) feeding would induce oral tolerance of either the T cell or B cell compartment in streptozotocin (STZ) diabetic rats. Experimental rats were fed 2 mg/kg of GAD (extracted from Escherichia coli) 14 days before being given intra-peritoneal injections of streptozotocin (STZ, 30 mg/kg body weight for 5 consecutive days). Of the two control groups, one was the diabetic control, which underwent STZ injections without being given the GAD; and the second was the normal control group. Systemic responses were compared between the three groups. T cell responses were assessed by a proliferation assay of spleen cells,...
Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2]... more Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2] (1), [Hg(2,2′-bipy)2(SCN)2] (2) and [Hg(phen)2(NO3)2] (3) have been synthesized and characterized by elemental analysis, IR, H NMR and C NMR spectroscopy. The thermal stability of 1–3 were studied by thermal gravimetric (TG) and differential thermal analyses (DTA). The structure of 1 has been confirmed by X-ray crystallography. The complex is a
Frozen sections of lymph nodes from normal mice were examined by immunofluorescence with anti-T l... more Frozen sections of lymph nodes from normal mice were examined by immunofluorescence with anti-T lymphocyte and anti-Ig reagents and by autoradiography following [3H]TdR administration. The scattered T lymphocytes present among B lymphocytes of primary follicles were found to be mostly nonproliferating; the few proliferating cells in primary follicles are therefore predominantly B lymphocytes. More than one-half (56%) of these proliferating B lymphocytes were found to be in direct contact with a T lymphocyte; this incidence is 5-fold higher than that expected by random association. In germinal centers, virtually all proliferating cells were found to be non-T lymphocytes as well. The association of proliferating B cells with T cells may be the result of specific cooperation between these two cell types. These data are discussed in the context of what is known of T/B collaboration and of primary follicles as a possible site of this interaction.
Bacillus Calmette-Guerin (BCG) is the only available vaccine against tuberculosis. The research f... more Bacillus Calmette-Guerin (BCG) is the only available vaccine against tuberculosis. The research for an improved vaccine is currently a very active field of investigation. In the present study, adjuvanticity effect of sterile sodium alginate on subcutaneous BCG vaccination in BALB/c mice was investigated. Mice were vaccinated subcutaneously with BCG plus alginate and the immune response and protective effect were compared to those of mice vaccinated with BCG alone by the same route. Proliferative and delayed-type hypersensitivity responses, IFN-gamma, specific anti-mycobacterium total IgG, IgG1 and IgG2a production were significantly higher in mice immunized subcutaneously with BCG plus alginate in comparison with results of mice immunized with BCG alone. Following systemic infection with BCG, mice vaccinated with BCG plus alginate had lower mean bacterial count compared to those vaccinated with BCG alone. The immune responses induced by subcutaneous administration of BCG plus alginate were significantly better than the responses induced by standard BCG vaccination.
Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2]... more Mercury(II) complexes of 1,10-phenanthroline (phen) and 2,2′-bipyridine (bipy), [Hg(phen)2(SCN)2] (1), [Hg(2,2′-bipy)2(SCN)2] (2) and [Hg(phen)2(NO3)2] (3) have been synthesized and characterized by elemental analysis, IR, H NMR and C NMR spectroscopy. The thermal stability of 1–3 were studied by thermal gravimetric (TG) and differential thermal analyses (DTA). The structure of 1 has been confirmed by X-ray crystallography. The complex is a
Background: Different methods have been used for BCG vaccination. Alginate microspheres are usefu... more Background: Different methods have been used for BCG vaccination. Alginate microspheres are useful in delivery of vaccines to the gastrointestinal tract by oral route. Objective: To compare the immune response following oral microencapsulated and ...
Autoimmune type 1 diabetes mellitus is caused by the T-cell mediated immune destruction of the in... more Autoimmune type 1 diabetes mellitus is caused by the T-cell mediated immune destruction of the insulin-producing beta-cell in pancreatic islets of Langerhans. The specificity of the auto-antibodies and of the auto-reactive T cells has been investigated, in which several auto-antigens were proposed. The purpose of this study was to determine whether glutamic acid decarboxylase (GAD) feeding would induce oral tolerance of either the T cell or B cell compartment in streptozotocin (STZ) diabetic rats. Experimental rats were fed 2 mg/kg of GAD (extracted from Escherichia coli) 14 days before being given intra-peritoneal injections of streptozotocin (STZ, 30 mg/kg body weight for 5 consecutive days). Of the two control groups, one was the diabetic control, which underwent STZ injections without being given the GAD; and the second was the normal control group. Systemic responses were compared between the three groups. T cell responses were assessed by a proliferation assay of spleen cells,...
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