A new compound containing a unique terpenoid-quinone skeleton, pycnanthuquinone C (1) along with ... more A new compound containing a unique terpenoid-quinone skeleton, pycnanthuquinone C (1) along with the known sargachromenol (2), prunetin (3), biochanin A (4), calopiptin (5), (12 S,13 S)-12,13-dihydroxylabda-8(17),14-dien-18-oic acid (6), (12 R,13 S)-12,13-dihydroxylabda-8(17),14-dien-18-oic acid (7), and sitosterol 3- O-beta- D-glucopyranoside (8), were isolated from the stem bark of Pycnanthus angolensis. Their structures were elucidated by spectroscopic means and comparison with published data. The antifungal activity of compounds 1, 2 and 3 was evaluated. Compound 1 was active against Trichophyton soudanense. Compound 2 was active against Trichophyton mentagrophytes while compound 3 was active against Microsporum audouinii and Trichophyton mentagrophytes.
International Journal of Analytical Chemistry, 2022
Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and... more Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and characteristic of dry leguminous forests. Different parts of this plant are used to treat wounds, skin infection, and to fight against cancer. Literature review indicated various pharmacological properties. Despite these medicinal properties, the chemical composition studies remain limited. This study aims to isolate and characterize secondary metabolites from P. africana leaves and evaluate their antibacterial and antioxidant properties. Air-dried powdered leaves of P. africana were macerated in methanol at room temperature and partitioned with ethyl acetate. The EtOAc extract was subjected successively to flash and column chromatographies in order to isolate compounds. The structure of the isolates was determined with help of spectroscopic data including 1D and 2D NMR experiments and comparison with literature data. The antibacterial activities were evaluated via determination of mini...
One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 kno... more One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 known compounds, heptacyclosordariolone (<b>2</b>) and sordariol (<b>3</b>), were isolated from an extract of <i>Sordaria</i> sp. AM-71, an endophytic fungus inhabiting <i>Garcinia polyantha</i>. The structures of these compounds were elucidated based on intensive nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry and by comparison with literature data. All compounds were assayed for their antimicrobial and cytotoxic properties. Only compound <b>2</b> showed antimicrobial activities against <i>Bacillus subtilis</i> DSMZ 704<i>, Pseudomonas agarici</i> DSMZ 11810, and <i>Micrococcus luteus</i> DSMZ 1605 with minimum inhibitory concentration values of 9.3, 15.5, and 16.9 µg/mL respectively. None of the isolated compounds showed a significant cytotoxic property.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
International Journal of Analytical Chemistry, 2022
Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and... more Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and characteristic of dry leguminous forests. Different parts of this plant are used to treat wounds, skin infection, and to fight against cancer. Literature review indicated various pharmacological properties. Despite these medicinal properties, the chemical composition studies remain limited. This study aims to isolate and characterize secondary metabolites from P. africana leaves and evaluate their antibacterial and antioxidant properties. Air-dried powdered leaves of P. africana were macerated in methanol at room temperature and partitioned with ethyl acetate. The EtOAc extract was subjected successively to flash and column chromatographies in order to isolate compounds. The structure of the isolates was determined with help of spectroscopic data including 1D and 2D NMR experiments and comparison with literature data. The antibacterial activities were evaluated via determination of mini...
One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 kno... more One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 known compounds, heptacyclosordariolone (<b>2</b>) and sordariol (<b>3</b>), were isolated from an extract of <i>Sordaria</i> sp. AM-71, an endophytic fungus inhabiting <i>Garcinia polyantha</i>. The structures of these compounds were elucidated based on intensive nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry and by comparison with literature data. All compounds were assayed for their antimicrobial and cytotoxic properties. Only compound <b>2</b> showed antimicrobial activities against <i>Bacillus subtilis</i> DSMZ 704<i>, Pseudomonas agarici</i> DSMZ 11810, and <i>Micrococcus luteus</i> DSMZ 1605 with minimum inhibitory concentration values of 9.3, 15.5, and 16.9 µg/mL respectively. None of the isolated compounds showed a significant cytotoxic property.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
A new compound, named banganxanthone C (=12-(1,1-dimethylprop-2-en-1-yl)-5,10-dihydroxy-9-methoxy... more A new compound, named banganxanthone C (=12-(1,1-dimethylprop-2-en-1-yl)-5,10-dihydroxy-9-methoxy-2-methyl-2-(4-methylpent-3-en-1-yl)-2H,6H-pyrano[3,2-b]xanthen-6-one; 4), together with five known compounds, were isolated from the leaves of Garcinia polyantha. The structures of the compounds were elucidated on the basis of 1D- and 2D-NMR spectroscopy. Among the known compounds, two were xanthones, one was a pentacyclic triterpene, one sterol, and one benzophenone derivative. Isoxanthochymol (2) and 4-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1,5,8-trihydroxy-3-methoxy-9H-xanthen-9-one (3) exhibited significant antiproliferative activity against the leukemia cell line TPH-1 with IC50 inhibition values of 1.5 and 2.8 μg/ml, respectively. The cytotoxic activity was found to be related to apoptosis induction.
Phytochemical investigation of the twigs of Garcinia nobilis led to the isolation of a new xantho... more Phytochemical investigation of the twigs of Garcinia nobilis led to the isolation of a new xanthone, named l-hydroxy-2,5-dimethoxyxanthone (1), together with 15 known compounds (2-16). The structures of the new and known compounds were established by means of spectroscopic methods and by comparison with previously reported data. The structure of compound 1 was confirmed by X-ray diffraction data. Compounds 1-16 were tested for their cytotoxic activity against human cervix carcinoma cell line KB-3-1. Compounds 5 and 11 showed moderate activity while others showed weak biological activity in these cytotoxicity assays. Compounds 4 and 9 were found to be inactive.
Supplemental material, online supplementary file 1, for Heptacyclosordarianone, a New Polyketide ... more Supplemental material, online supplementary file 1, for Heptacyclosordarianone, a New Polyketide From <i>Sordaria</i> sp., an Endophytic Fungus From <i>Garcinia polyantha</i> by Virginie Flaure Tsague Tankeu, Denis Kehdinga Sema, Jean-Bosco Jouda, Simplice Joel N. Tatsimo, Patrick Akono Ntonga, Jean Duplex Wansi, Simeon Kouam Fogue, Benoît Loura, Yoshihito Shiono, Norbert Sewald and Alain Meli Lannang in Natural Product Communications
One new depsidone, polyanthadepsidone A (<b>1</b>), together with four known compound... more One new depsidone, polyanthadepsidone A (<b>1</b>), together with four known compounds were isolated from the dichloromethane extract of the leaves of <i>Garcinia polyantha</i>. The structures of all compounds were determined by comprehensive analyses of their 1D and 2D NMR and EI mass spectral data. All the isolates exhibited suppressive effect on phagocytosis response upon activation with serum opsonised zymosan in the IC<sub>50</sub> range of 4.5–23.80 μM, tested <i>in vitro</i> for oxidative burst studies of whole blood.
Two new (1-2) and eight known (3-10) secondary metabolites were isolated from the rhizomes of Kni... more Two new (1-2) and eight known (3-10) secondary metabolites were isolated from the rhizomes of Kniphofia reflexa Hutchinson ex Codd. Among them, an indane derivative kniphofiarindane (1), and naphthalene derivative kniphofiarexine (2), were identified as new natural products. 1D-and 2D-NMR spectroscopic studies together with single-crystal X-ray diffraction techniques were employed in the structural elucidation of the new compounds, 1 and 2. These compounds were evaluated for their cytotoxic effect against kidney epithelial cell line (LLC-MK2). Compound 3 was found to be highly cytotoxic, while compounds 1, and 10 showed moderate cytotoxicity with CC 50 values of 4.44 ± 0.79, 16.35 ± 1.54, and 11.24 ± 1.22 μg/mL, respectively against the tested standard Gleevec (Imatinib), (CC 50 = 18.50 ± 1.21 μg/mL). Compounds 8, and 9 also showed moderate anti-inflammatory activity (CC 50 = 38.7 ± 4.90, and 20.00 ± 4.40 μg/mL, respectively) against ROS production .
A new compound containing a unique terpenoid-quinone skeleton, pycnanthuquinone C (1) along with ... more A new compound containing a unique terpenoid-quinone skeleton, pycnanthuquinone C (1) along with the known sargachromenol (2), prunetin (3), biochanin A (4), calopiptin (5), (12 S,13 S)-12,13-dihydroxylabda-8(17),14-dien-18-oic acid (6), (12 R,13 S)-12,13-dihydroxylabda-8(17),14-dien-18-oic acid (7), and sitosterol 3- O-beta- D-glucopyranoside (8), were isolated from the stem bark of Pycnanthus angolensis. Their structures were elucidated by spectroscopic means and comparison with published data. The antifungal activity of compounds 1, 2 and 3 was evaluated. Compound 1 was active against Trichophyton soudanense. Compound 2 was active against Trichophyton mentagrophytes while compound 3 was active against Microsporum audouinii and Trichophyton mentagrophytes.
International Journal of Analytical Chemistry, 2022
Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and... more Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and characteristic of dry leguminous forests. Different parts of this plant are used to treat wounds, skin infection, and to fight against cancer. Literature review indicated various pharmacological properties. Despite these medicinal properties, the chemical composition studies remain limited. This study aims to isolate and characterize secondary metabolites from P. africana leaves and evaluate their antibacterial and antioxidant properties. Air-dried powdered leaves of P. africana were macerated in methanol at room temperature and partitioned with ethyl acetate. The EtOAc extract was subjected successively to flash and column chromatographies in order to isolate compounds. The structure of the isolates was determined with help of spectroscopic data including 1D and 2D NMR experiments and comparison with literature data. The antibacterial activities were evaluated via determination of mini...
One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 kno... more One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 known compounds, heptacyclosordariolone (<b>2</b>) and sordariol (<b>3</b>), were isolated from an extract of <i>Sordaria</i> sp. AM-71, an endophytic fungus inhabiting <i>Garcinia polyantha</i>. The structures of these compounds were elucidated based on intensive nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry and by comparison with literature data. All compounds were assayed for their antimicrobial and cytotoxic properties. Only compound <b>2</b> showed antimicrobial activities against <i>Bacillus subtilis</i> DSMZ 704<i>, Pseudomonas agarici</i> DSMZ 11810, and <i>Micrococcus luteus</i> DSMZ 1605 with minimum inhibitory concentration values of 9.3, 15.5, and 16.9 µg/mL respectively. None of the isolated compounds showed a significant cytotoxic property.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
International Journal of Analytical Chemistry, 2022
Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and... more Prosopis africana (G. &Perr.) Taub (Mimosaceae) is a large tree native to dry tropical Africa and characteristic of dry leguminous forests. Different parts of this plant are used to treat wounds, skin infection, and to fight against cancer. Literature review indicated various pharmacological properties. Despite these medicinal properties, the chemical composition studies remain limited. This study aims to isolate and characterize secondary metabolites from P. africana leaves and evaluate their antibacterial and antioxidant properties. Air-dried powdered leaves of P. africana were macerated in methanol at room temperature and partitioned with ethyl acetate. The EtOAc extract was subjected successively to flash and column chromatographies in order to isolate compounds. The structure of the isolates was determined with help of spectroscopic data including 1D and 2D NMR experiments and comparison with literature data. The antibacterial activities were evaluated via determination of mini...
One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 kno... more One new polyketide derivative, heptacyclosordarianone (<b>1</b>), together with 2 known compounds, heptacyclosordariolone (<b>2</b>) and sordariol (<b>3</b>), were isolated from an extract of <i>Sordaria</i> sp. AM-71, an endophytic fungus inhabiting <i>Garcinia polyantha</i>. The structures of these compounds were elucidated based on intensive nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry and by comparison with literature data. All compounds were assayed for their antimicrobial and cytotoxic properties. Only compound <b>2</b> showed antimicrobial activities against <i>Bacillus subtilis</i> DSMZ 704<i>, Pseudomonas agarici</i> DSMZ 11810, and <i>Micrococcus luteus</i> DSMZ 1605 with minimum inhibitory concentration values of 9.3, 15.5, and 16.9 µg/mL respectively. None of the isolated compounds showed a significant cytotoxic property.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
A new compound, named banganxanthone C (=12-(1,1-dimethylprop-2-en-1-yl)-5,10-dihydroxy-9-methoxy... more A new compound, named banganxanthone C (=12-(1,1-dimethylprop-2-en-1-yl)-5,10-dihydroxy-9-methoxy-2-methyl-2-(4-methylpent-3-en-1-yl)-2H,6H-pyrano[3,2-b]xanthen-6-one; 4), together with five known compounds, were isolated from the leaves of Garcinia polyantha. The structures of the compounds were elucidated on the basis of 1D- and 2D-NMR spectroscopy. Among the known compounds, two were xanthones, one was a pentacyclic triterpene, one sterol, and one benzophenone derivative. Isoxanthochymol (2) and 4-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-1,5,8-trihydroxy-3-methoxy-9H-xanthen-9-one (3) exhibited significant antiproliferative activity against the leukemia cell line TPH-1 with IC50 inhibition values of 1.5 and 2.8 μg/ml, respectively. The cytotoxic activity was found to be related to apoptosis induction.
Phytochemical investigation of the twigs of Garcinia nobilis led to the isolation of a new xantho... more Phytochemical investigation of the twigs of Garcinia nobilis led to the isolation of a new xanthone, named l-hydroxy-2,5-dimethoxyxanthone (1), together with 15 known compounds (2-16). The structures of the new and known compounds were established by means of spectroscopic methods and by comparison with previously reported data. The structure of compound 1 was confirmed by X-ray diffraction data. Compounds 1-16 were tested for their cytotoxic activity against human cervix carcinoma cell line KB-3-1. Compounds 5 and 11 showed moderate activity while others showed weak biological activity in these cytotoxicity assays. Compounds 4 and 9 were found to be inactive.
Supplemental material, online supplementary file 1, for Heptacyclosordarianone, a New Polyketide ... more Supplemental material, online supplementary file 1, for Heptacyclosordarianone, a New Polyketide From <i>Sordaria</i> sp., an Endophytic Fungus From <i>Garcinia polyantha</i> by Virginie Flaure Tsague Tankeu, Denis Kehdinga Sema, Jean-Bosco Jouda, Simplice Joel N. Tatsimo, Patrick Akono Ntonga, Jean Duplex Wansi, Simeon Kouam Fogue, Benoît Loura, Yoshihito Shiono, Norbert Sewald and Alain Meli Lannang in Natural Product Communications
One new depsidone, polyanthadepsidone A (<b>1</b>), together with four known compound... more One new depsidone, polyanthadepsidone A (<b>1</b>), together with four known compounds were isolated from the dichloromethane extract of the leaves of <i>Garcinia polyantha</i>. The structures of all compounds were determined by comprehensive analyses of their 1D and 2D NMR and EI mass spectral data. All the isolates exhibited suppressive effect on phagocytosis response upon activation with serum opsonised zymosan in the IC<sub>50</sub> range of 4.5–23.80 μM, tested <i>in vitro</i> for oxidative burst studies of whole blood.
Two new (1-2) and eight known (3-10) secondary metabolites were isolated from the rhizomes of Kni... more Two new (1-2) and eight known (3-10) secondary metabolites were isolated from the rhizomes of Kniphofia reflexa Hutchinson ex Codd. Among them, an indane derivative kniphofiarindane (1), and naphthalene derivative kniphofiarexine (2), were identified as new natural products. 1D-and 2D-NMR spectroscopic studies together with single-crystal X-ray diffraction techniques were employed in the structural elucidation of the new compounds, 1 and 2. These compounds were evaluated for their cytotoxic effect against kidney epithelial cell line (LLC-MK2). Compound 3 was found to be highly cytotoxic, while compounds 1, and 10 showed moderate cytotoxicity with CC 50 values of 4.44 ± 0.79, 16.35 ± 1.54, and 11.24 ± 1.22 μg/mL, respectively against the tested standard Gleevec (Imatinib), (CC 50 = 18.50 ± 1.21 μg/mL). Compounds 8, and 9 also showed moderate anti-inflammatory activity (CC 50 = 38.7 ± 4.90, and 20.00 ± 4.40 μg/mL, respectively) against ROS production .
Uploads
Papers by Simplice N Tatsimo