Pinus sylvestris (Scots pine) is one of the most widespread conifer in the world occupying 37% of... more Pinus sylvestris (Scots pine) is one of the most widespread conifer in the world occupying 37% of forest area and has relict populations from the Pleistocene in the Mediterranean region and central Europe. Variation in mitochondrial DNA (maternally inherited in pines) has indicated that the three major mitotypes are present in Europe, as well as microsatellite markers have revealed the expected heterozygosities to be between 0.50-0.80 (Keys et al ., 2000). In central and eastern Europe the gene pools of Scots pine were formed in postglacial times during the migration of the species from the glacial refugia and intensive gene exchange between populations and more recently by human activities. Some studies on macro and mega fossil data (Willis & van Andel, 2004) suggest that Scots pine survived in central Europe more than 30.000 years ago. Two mitochondrial markers nad 1 intronB/C, nad 7 intron1 and 9 SSR nuclear markers were used to determine the genetic variability and post-glacial ...
Fiber (tracheid) length is an important trait targeted for genetic and silvicultural improvement.... more Fiber (tracheid) length is an important trait targeted for genetic and silvicultural improvement. Such studies require large-scale non-destructive sampling, and accurate length determination. The standard procedure for non-destructive sampling is to collect increment cores, singularize their cells by maceration, measure them with optical analyzer and apply various corrections to suppress influence of non-fiber particles and cut fibers, as fibers are cut by the corer. The recently developed expectation-maximization method (EM) not only addresses the problem of non-fibers and cut fibers, but also corrects for the sampling bias. Here, the performance of the EM method has been evaluated by comparing it with length-weighing and squared length-weighing, both implemented in fiber analyzers, and with microscopy data for intact fibers, corrected for sampling bias, as the reference. This was done for 12-mm increment cores from 16 Norway spruce (
Somatic mutations are transmitted during mitosis in developing somatic tissue. Somatic cells bear... more Somatic mutations are transmitted during mitosis in developing somatic tissue. Somatic cells bearing the mutations can develop into reproductive (germ) cells and the somatic mutations are then passed on to the next generation of plants. Somatic mutations are a source of variation essential to evolve new defense strategies and adapt to the environment. Stem rust disease in Scots pine has a negative effect on wood quality, and thus adversely affects the economy. It is caused by the 2 most destructive fungal species in Scandinavia: Peridermium pini and Cronartium flaccidum. We studied nuclear genome stability in Scots pine under biotic stress (fungus-infected, 22 trees) compared to a control population (plantation, 20 trees). Stability was assessed as accumulation of new somatic mutations in 10 microsatellite loci selected for genotyping. Microsatellites are widely used as molecular markers in population genetics studies of plants, and are particularly used for detection of somatic mut...
Pinus sylvestris (Scots pine) is one of the most widespread conifer in the world occupying 37% of... more Pinus sylvestris (Scots pine) is one of the most widespread conifer in the world occupying 37% of forest area and has relict populations from the Pleistocene in the Mediterranean region and central Europe. Variation in mitochondrial DNA (maternally inherited in pines) has indicated that the three major mitotypes are present in Europe, as well as microsatellite markers have revealed the expected heterozygosities to be between 0.50-0.80 (Keys et al ., 2000). In central and eastern Europe the gene pools of Scots pine were formed in postglacial times during the migration of the species from the glacial refugia and intensive gene exchange between populations and more recently by human activities. Some studies on macro and mega fossil data (Willis & van Andel, 2004) suggest that Scots pine survived in central Europe more than 30.000 years ago. Two mitochondrial markers nad 1 intronB/C, nad 7 intron1 and 9 SSR nuclear markers were used to determine the genetic variability and post-glacial ...
Fiber (tracheid) length is an important trait targeted for genetic and silvicultural improvement.... more Fiber (tracheid) length is an important trait targeted for genetic and silvicultural improvement. Such studies require large-scale non-destructive sampling, and accurate length determination. The standard procedure for non-destructive sampling is to collect increment cores, singularize their cells by maceration, measure them with optical analyzer and apply various corrections to suppress influence of non-fiber particles and cut fibers, as fibers are cut by the corer. The recently developed expectation-maximization method (EM) not only addresses the problem of non-fibers and cut fibers, but also corrects for the sampling bias. Here, the performance of the EM method has been evaluated by comparing it with length-weighing and squared length-weighing, both implemented in fiber analyzers, and with microscopy data for intact fibers, corrected for sampling bias, as the reference. This was done for 12-mm increment cores from 16 Norway spruce (
Somatic mutations are transmitted during mitosis in developing somatic tissue. Somatic cells bear... more Somatic mutations are transmitted during mitosis in developing somatic tissue. Somatic cells bearing the mutations can develop into reproductive (germ) cells and the somatic mutations are then passed on to the next generation of plants. Somatic mutations are a source of variation essential to evolve new defense strategies and adapt to the environment. Stem rust disease in Scots pine has a negative effect on wood quality, and thus adversely affects the economy. It is caused by the 2 most destructive fungal species in Scandinavia: Peridermium pini and Cronartium flaccidum. We studied nuclear genome stability in Scots pine under biotic stress (fungus-infected, 22 trees) compared to a control population (plantation, 20 trees). Stability was assessed as accumulation of new somatic mutations in 10 microsatellite loci selected for genotyping. Microsatellites are widely used as molecular markers in population genetics studies of plants, and are particularly used for detection of somatic mut...
Uploads
Papers by Stefana Ganea