Endoderm originates from a large endomesodermal field requiring Nodal signalling. The mechanisms ... more Endoderm originates from a large endomesodermal field requiring Nodal signalling. The mechanisms that ensure segregation of endoderm from mesoderm are not fully understood. We first show that the timing and dose of Nodal activation are crucial for endoderm formation and the endoderm versus mesoderm fate choice, because sustained Nodal signalling is required to ensure endoderm formation but transient signalling is sufficient for mesoderm formation. In zebrafish, downstream of Nodal signals, three genes encoding transcription factors (faust, bonnie and clyde and the recently identified gene casanova) are required for endoderm formation and differentiation. However their positions within the pathway are not completely established. In the present work, we show that casanova is the earliest specification marker for endodermal cells and that its expression requires bonnie and clyde. Furthermore, we have analysed the molecular activities of casanova on endoderm formation and found that it ...
... 3A–C). These include primary Rohon Beard sensory neurons and motor neurons of the spinal cord... more ... 3A–C). These include primary Rohon Beard sensory neurons and motor neurons of the spinal cord (Fig. ... In the spinal cord of the 24 h old embryo, motor neurons and some interneurons, in addition to the dorsally located Rohon Beard neurons, express bck mRNA weakly (Fig. ...
Endoderm originates from a large endomesodermal field requiring Nodal signalling. The mechanisms ... more Endoderm originates from a large endomesodermal field requiring Nodal signalling. The mechanisms that ensure segregation of endoderm from mesoderm are not fully understood. We first show that the timing and dose of Nodal activation are crucial for endoderm formation and the endoderm versus mesoderm fate choice, because sustained Nodal signalling is required to ensure endoderm formation but transient signalling is sufficient for mesoderm formation. In zebrafish, downstream of Nodal signals, three genes encoding transcription factors (faust, bonnie and clyde and the recently identified gene casanova) are required for endoderm formation and differentiation. However their positions within the pathway are not completely established. In the present work, we show that casanova is the earliest specification marker for endodermal cells and that its expression requires bonnie and clyde. Furthermore, we have analysed the molecular activities of casanova on endoderm formation and found that it ...
... 3A–C). These include primary Rohon Beard sensory neurons and motor neurons of the spinal cord... more ... 3A–C). These include primary Rohon Beard sensory neurons and motor neurons of the spinal cord (Fig. ... In the spinal cord of the 24 h old embryo, motor neurons and some interneurons, in addition to the dorsally located Rohon Beard neurons, express bck mRNA weakly (Fig. ...
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Papers by Thomas Dickmeis