Amniocentesis and prenatal diagnosis were done for late maternal age and an abnormality consisten... more Amniocentesis and prenatal diagnosis were done for late maternal age and an abnormality consistent with Tetrasomy 21 (47,XX,+t(21;21)) was found in every cell examined of the initial amniotic fluid. Clinical examination revealed a fetus with many of the signs of Down Syndrome and pathological examination revealed gross abnormalities of the internal structures. Follow-up tissues showed mosaic Tetrasomy 21.
The protection elicited by a temperature-sensitive (Ts) mutant of Ornithobacterium rhinotracheale... more The protection elicited by a temperature-sensitive (Ts) mutant of Ornithobacterium rhinotracheale (ORT) vaccine against challenge with pathogenic strain was investigated. In Experiment 1, specific serologic response to ORT was detected in 12%-19% of Ts-vaccinated birds at 3 wk postvaccination by either drinking water or oculo-nasal instillation. At 7 days postchallenge, 100% of Ts-vaccinated turkeys of all groups were able to respond with an ORT-specific antibody response, but the control group was not, suggesting the potential of Ts strain to evoke immune protection. The study also revealed a statistically significant ability of the Ts strain to protect vaccinated turkeys against gross lesions caused by the pathogenic strain of ORT in treated groups vs. control. In Experiment 2, seroconversion was detected by enzyme-linked immunosorbent assay in birds after they were given the Ts strain in drinking water in field conditions. The results of the field study showed mean scores of gross lesions of nonvaccinated/challenged groups to be up to seven times higher than those of the vaccinated/challenged group. In addition, reisolation rates and quantification of ORT colonies per gram of lung tissue were significantly lower for vaccinated/challenged than for nonvaccinated/challenged turkeys. In conclusion, results from laboratory and field experiments suggest that use of the Ts mutant strain of ORT as a live vaccine would be a suitable method to evoke protection against ORT infection in turkeys.
The objective of this research was to study the harvest moisture and drying method systems as a f... more The objective of this research was to study the harvest moisture and drying method systems as a function to storage period on the conservability and technological quality of wheat. Grains of wheat (Triticum aestivum L), cultivar 'Embrapa 16', produced in the Granja Bretanhas, in Jaguarão, RS, agricultural harvest of 1997/1998 were harvested dry in the plant (14% of moisture), with 16 and 18% of moisture. The samples harvested with moisture of 16 and 18% were submitted to artificial drying by intermittent method, with air at 70°C, stationary, with air at 45°C; and stationary, at room temperature. The grains were stored in a conventional system, during 12 months, with the realization of evaluations at 4 month intervals. Wheat grains harvested dry shown inferior technological quality and conservability in storage as compared to harvested with moisture above 14% and dry at artificial dryer.
Amniocentesis and prenatal diagnosis were done for late maternal age and an abnormality consisten... more Amniocentesis and prenatal diagnosis were done for late maternal age and an abnormality consistent with Tetrasomy 21 (47,XX,+t(21;21)) was found in every cell examined of the initial amniotic fluid. Clinical examination revealed a fetus with many of the signs of Down Syndrome and pathological examination revealed gross abnormalities of the internal structures. Follow-up tissues showed mosaic Tetrasomy 21.
The protection elicited by a temperature-sensitive (Ts) mutant of Ornithobacterium rhinotracheale... more The protection elicited by a temperature-sensitive (Ts) mutant of Ornithobacterium rhinotracheale (ORT) vaccine against challenge with pathogenic strain was investigated. In Experiment 1, specific serologic response to ORT was detected in 12%-19% of Ts-vaccinated birds at 3 wk postvaccination by either drinking water or oculo-nasal instillation. At 7 days postchallenge, 100% of Ts-vaccinated turkeys of all groups were able to respond with an ORT-specific antibody response, but the control group was not, suggesting the potential of Ts strain to evoke immune protection. The study also revealed a statistically significant ability of the Ts strain to protect vaccinated turkeys against gross lesions caused by the pathogenic strain of ORT in treated groups vs. control. In Experiment 2, seroconversion was detected by enzyme-linked immunosorbent assay in birds after they were given the Ts strain in drinking water in field conditions. The results of the field study showed mean scores of gross lesions of nonvaccinated/challenged groups to be up to seven times higher than those of the vaccinated/challenged group. In addition, reisolation rates and quantification of ORT colonies per gram of lung tissue were significantly lower for vaccinated/challenged than for nonvaccinated/challenged turkeys. In conclusion, results from laboratory and field experiments suggest that use of the Ts mutant strain of ORT as a live vaccine would be a suitable method to evoke protection against ORT infection in turkeys.
The objective of this research was to study the harvest moisture and drying method systems as a f... more The objective of this research was to study the harvest moisture and drying method systems as a function to storage period on the conservability and technological quality of wheat. Grains of wheat (Triticum aestivum L), cultivar 'Embrapa 16', produced in the Granja Bretanhas, in Jaguarão, RS, agricultural harvest of 1997/1998 were harvested dry in the plant (14% of moisture), with 16 and 18% of moisture. The samples harvested with moisture of 16 and 18% were submitted to artificial drying by intermittent method, with air at 70°C, stationary, with air at 45°C; and stationary, at room temperature. The grains were stored in a conventional system, during 12 months, with the realization of evaluations at 4 month intervals. Wheat grains harvested dry shown inferior technological quality and conservability in storage as compared to harvested with moisture above 14% and dry at artificial dryer.
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