Morphological variation in natural populations is a genomic test bed for studying the interface b... more Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy's cat (Leopardus geoffroyi). To assess population level variation in the regions surrounding the causative mutations we apply genomic resources from the domestic cat to carry out clone-based capture and targeted resequencing of 299 kb and 251 kb segments that contain ASIP and MC1R, respectively, from...
A quantitative polymerase chain reaction (PCR) assay has been developed for the quantification of... more A quantitative polymerase chain reaction (PCR) assay has been developed for the quantification of genomic DNA extracted from domestic cat samples. The assay, which targets highly repetitive genomic short interspersed nuclear elements (SINE), can be performed rapidly and is highly sensitive, detecting as little as 10 fg of feline genomic DNA. The assay was linear over a 10(6) dilution range. We have recently developed a short tandem repeat (STR) multiplex panel for forensic analysis of feline specimens. The SINE assay is an integral part of the forensic typing system. The sensitivity of the assay will enable forensic examiners to determine the likelihood of success of genotyping sample extracts with the STR panel without sacrificing valuable DNA necessary to perform genotyping of samples.
The Dominant White locus (W) in the domestic cat demonstrates pleiotropic effects exhibiting comp... more The Dominant White locus (W) in the domestic cat demonstrates pleiotropic effects exhibiting complete penetrance for absence of coat pigmentation and incomplete penetrance for deafness and iris hypopigmentation. We performed linkage analysis using a pedigree segregating White to identify KIT (Chr. B1) as the feline W locus. Segregation and sequence analysis of the KIT gene in two pedigrees (P1 and P2) revealed the remarkable retrotransposition and evolution of a feline endogenous retrovirus (FERV1) as responsible for two distinct phenotypes of the W locus, Dominant White, and white spotting. A full-length (7125 bp) FERV1 element is associated with white spotting, whereas a FERV1 long terminal repeat (LTR) is associated with all Dominant White individuals. For purposes of statistical analysis, the alternatives of wild-type sequence, FERV1 element, and LTR-only define a triallelic marker. Taking into account pedigree relationships, deafness is genetically linked and associated with th...
Title: Pet cat hair implicates murder suspect. Authors: Menotti-Raymond, Marilyn A.; David, Victo... more Title: Pet cat hair implicates murder suspect. Authors: Menotti-Raymond, Marilyn A.; David, Victor A.; O'Brien, Stephen J. Affiliation: AA(Laboratory of Genomic Diversity and *SAIC, National Cancer Institute, Frederick, Maryland ...
... Development of microsatellite markers in the guanaco, Lama guanicoe: utility for South Americ... more ... Development of microsatellite markers in the guanaco, Lama guanicoe: utility for South American camelids. Ronald J. Sarno,; Victor A. David,; William L. Franklin,; Stephen J. O'Brien,; Warren E. Johnson. Article first published online: 25 DEC 2001. ...
Morphological variation in natural populations is a genomic test bed for studying the interface b... more Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy's cat (Leopardus geoffroyi). To assess population level variation in the regions surrounding the causative mutations we apply genomic resources from the domestic cat to carry out clone-based capture and targeted resequencing of 299 kb and 251 kb segments that contain ASIP and MC1R, respectively, from...
A quantitative polymerase chain reaction (PCR) assay has been developed for the quantification of... more A quantitative polymerase chain reaction (PCR) assay has been developed for the quantification of genomic DNA extracted from domestic cat samples. The assay, which targets highly repetitive genomic short interspersed nuclear elements (SINE), can be performed rapidly and is highly sensitive, detecting as little as 10 fg of feline genomic DNA. The assay was linear over a 10(6) dilution range. We have recently developed a short tandem repeat (STR) multiplex panel for forensic analysis of feline specimens. The SINE assay is an integral part of the forensic typing system. The sensitivity of the assay will enable forensic examiners to determine the likelihood of success of genotyping sample extracts with the STR panel without sacrificing valuable DNA necessary to perform genotyping of samples.
The Dominant White locus (W) in the domestic cat demonstrates pleiotropic effects exhibiting comp... more The Dominant White locus (W) in the domestic cat demonstrates pleiotropic effects exhibiting complete penetrance for absence of coat pigmentation and incomplete penetrance for deafness and iris hypopigmentation. We performed linkage analysis using a pedigree segregating White to identify KIT (Chr. B1) as the feline W locus. Segregation and sequence analysis of the KIT gene in two pedigrees (P1 and P2) revealed the remarkable retrotransposition and evolution of a feline endogenous retrovirus (FERV1) as responsible for two distinct phenotypes of the W locus, Dominant White, and white spotting. A full-length (7125 bp) FERV1 element is associated with white spotting, whereas a FERV1 long terminal repeat (LTR) is associated with all Dominant White individuals. For purposes of statistical analysis, the alternatives of wild-type sequence, FERV1 element, and LTR-only define a triallelic marker. Taking into account pedigree relationships, deafness is genetically linked and associated with th...
Title: Pet cat hair implicates murder suspect. Authors: Menotti-Raymond, Marilyn A.; David, Victo... more Title: Pet cat hair implicates murder suspect. Authors: Menotti-Raymond, Marilyn A.; David, Victor A.; O'Brien, Stephen J. Affiliation: AA(Laboratory of Genomic Diversity and *SAIC, National Cancer Institute, Frederick, Maryland ...
... Development of microsatellite markers in the guanaco, Lama guanicoe: utility for South Americ... more ... Development of microsatellite markers in the guanaco, Lama guanicoe: utility for South American camelids. Ronald J. Sarno,; Victor A. David,; William L. Franklin,; Stephen J. O'Brien,; Warren E. Johnson. Article first published online: 25 DEC 2001. ...
Uploads
Papers by Victor David