HPV-16 is the most prevalent human papillomavirus genotype found in cervical intraepithelial neop... more HPV-16 is the most prevalent human papillomavirus genotype found in cervical intraepithelial neoplasias. The regulatory region of the HPV genome, LCR, has several binding sites for cellular and viral transcription factors, and nucleotide substitutions in this genomic region can interfere with the viral oncogenic expression. The present study aims to determine the LCR variability of European and non-European HPV-16 variants found in Brazil. Through automated sequencing, it was possible to characterize the LCR of ten non-European (eight Asian-American, one African 1, one African 2) and twelve European isolates. Among the 22 isolates analyzed, nine may be new variants of HPV-16, with different combinations of previously reported nucleotide substitutions, and three showed new substitutions not previously reported. Two new nucleotide substitutions, the insertion of T at position 7621 and the substitution of A to G at position 7836, were found in a single isolate, Bsb-14, a putative new African 1 variant. The characterization of the LCR of human papillomaviruses can be of pivotal importance to the understanding of the viral replication and pathogenicity.
Geminiviridae is the largest family of plant DNA viruses that infect a broad range of plants caus... more Geminiviridae is the largest family of plant DNA viruses that infect a broad range of plants causing a limitation to the production of economically important or staple food crops within tropical and subtropical countries. Increasing knowledge about its epidemiology, sequence diversity and biodiversity is highly important in order to implement preventative strategies. With the use of rolling circle amplification (RCA) combined with restriction fragment length polymorphism (RFLP), in addition to other methods applied in molecular biology, technical improvements on direct sequencing, shot gun cloning and pyrosequencing were achieved in this work. In the first two parts of this study RCA/RFLP was used to diagnose geminiviral infection in symptomatic plant samples originally collected in South America. All samples from Bolivia, in total 7 weeds and 21 of 22 samples from Brazil, including beans samples, were diagnosed virus positive when analyzed using RCA/RFLP. RCA products were sequence...
Asystasia gangetica (Acanthaceae) from tropical Africa and Asia is used as source of food and for... more Asystasia gangetica (Acanthaceae) from tropical Africa and Asia is used as source of food and for medical applications. Plants collected in West Africa in the 1980s with typical geminivirus symptoms showed an unusual symptom segregation that included vein yellowing, curling and mosaic, which were present simultaneously or separately on different leaves of the same plant or on different plants propagated as cuttings from a single plant. Rolling-circle amplification in combination with restriction fragment length polymorphism analysis followed by deep sequencing of the RCA products identified two geminiviruses in these plants. One with a bipartite genome, Asystasia begomovirus 1, and the other with a monopartite genome together with its defective DNA, Asystasia begomovirus 2. The relationship between leaf symptoms and virus distribution under different light regimes was investigated, and showed for the first time an unusual segregation of symptoms and viruses, either within a single p...
... Regina MS Amorim/++, Claudiner P Oliveira/++, Patrícia S Wyant/++, Daniela M Cerqueira/++, Ge... more ... Regina MS Amorim/++, Claudiner P Oliveira/++, Patrícia S Wyant/++, Daniela M Cerqueira/++, Geni NL Câmara*, Luciano S Flores**, Regina MB Martins***, Cláudia RF Martins/+ ... Braz J Med Biol Res 30: 339-345. Gonçales NSL, Costa FF, Vassallo Jr J, Gonçales FL 2000. ...
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors... more The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.
HPV-16 is the most prevalent human pap-illomavirus genotype found in cervical intraepithelial neo... more HPV-16 is the most prevalent human pap-illomavirus genotype found in cervical intraepithelial neoplasias. The regulatory region of the HPV genome, LCR, has several binding sites for cellular and viral transcription factors, and nucleotide substitutions in this genomic region can ...
Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for International Jou... more Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for International Journal of Gynecological Cancer. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information ...
HPV-16 is the most prevalent human papillomavirus genotype found in cervical intraepithelial neop... more HPV-16 is the most prevalent human papillomavirus genotype found in cervical intraepithelial neoplasias. The regulatory region of the HPV genome, LCR, has several binding sites for cellular and viral transcription factors, and nucleotide substitutions in this genomic region can interfere with the viral oncogenic expression. The present study aims to determine the LCR variability of European and non-European HPV-16 variants found in Brazil. Through automated sequencing, it was possible to characterize the LCR of ten non-European (eight Asian-American, one African 1, one African 2) and twelve European isolates. Among the 22 isolates analyzed, nine may be new variants of HPV-16, with different combinations of previously reported nucleotide substitutions, and three showed new substitutions not previously reported. Two new nucleotide substitutions, the insertion of T at position 7621 and the substitution of A to G at position 7836, were found in a single isolate, Bsb-14, a putative new African 1 variant. The characterization of the LCR of human papillomaviruses can be of pivotal importance to the understanding of the viral replication and pathogenicity.
Geminiviridae is the largest family of plant DNA viruses that infect a broad range of plants caus... more Geminiviridae is the largest family of plant DNA viruses that infect a broad range of plants causing a limitation to the production of economically important or staple food crops within tropical and subtropical countries. Increasing knowledge about its epidemiology, sequence diversity and biodiversity is highly important in order to implement preventative strategies. With the use of rolling circle amplification (RCA) combined with restriction fragment length polymorphism (RFLP), in addition to other methods applied in molecular biology, technical improvements on direct sequencing, shot gun cloning and pyrosequencing were achieved in this work. In the first two parts of this study RCA/RFLP was used to diagnose geminiviral infection in symptomatic plant samples originally collected in South America. All samples from Bolivia, in total 7 weeds and 21 of 22 samples from Brazil, including beans samples, were diagnosed virus positive when analyzed using RCA/RFLP. RCA products were sequence...
Asystasia gangetica (Acanthaceae) from tropical Africa and Asia is used as source of food and for... more Asystasia gangetica (Acanthaceae) from tropical Africa and Asia is used as source of food and for medical applications. Plants collected in West Africa in the 1980s with typical geminivirus symptoms showed an unusual symptom segregation that included vein yellowing, curling and mosaic, which were present simultaneously or separately on different leaves of the same plant or on different plants propagated as cuttings from a single plant. Rolling-circle amplification in combination with restriction fragment length polymorphism analysis followed by deep sequencing of the RCA products identified two geminiviruses in these plants. One with a bipartite genome, Asystasia begomovirus 1, and the other with a monopartite genome together with its defective DNA, Asystasia begomovirus 2. The relationship between leaf symptoms and virus distribution under different light regimes was investigated, and showed for the first time an unusual segregation of symptoms and viruses, either within a single p...
... Regina MS Amorim/++, Claudiner P Oliveira/++, Patrícia S Wyant/++, Daniela M Cerqueira/++, Ge... more ... Regina MS Amorim/++, Claudiner P Oliveira/++, Patrícia S Wyant/++, Daniela M Cerqueira/++, Geni NL Câmara*, Luciano S Flores**, Regina MB Martins***, Cláudia RF Martins/+ ... Braz J Med Biol Res 30: 339-345. Gonçales NSL, Costa FF, Vassallo Jr J, Gonçales FL 2000. ...
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors... more The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.
HPV-16 is the most prevalent human pap-illomavirus genotype found in cervical intraepithelial neo... more HPV-16 is the most prevalent human pap-illomavirus genotype found in cervical intraepithelial neoplasias. The regulatory region of the HPV genome, LCR, has several binding sites for cellular and viral transcription factors, and nucleotide substitutions in this genomic region can ...
Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for International Jou... more Close Window. Close Window. Thank you for choosing to subscribe to the eTOC for International Journal of Gynecological Cancer. Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information ...
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