Oxidative stress is a state related to an increased cellular damage produced by oxygen and oxygen... more Oxidative stress is a state related to an increased cellular damage produced by oxygen and oxygen derived free radicals known as reactive oxygen species (ROS). It is a serious condition, as ROS and their metabolites attack DNA, lipids and proteins, alter enzymatic systems and cell signalizing pathways producing irreparable alterations, cell death and necrosis. While small amounts of ROS have been shown to be required for some physiological functions, their excessive levels can impair an overall fertilization capacity. This questions have recently attracted the attention of the scientific society, however, research in exploring the role of oxidative stress and antioxidants associated with fertility remains in his initial stages and therefore needs further exploration. This review summarizes the latest consensus on the role of ROS and antioxidants in reproduction. It should not only help to clarify the role of ROS and antioxidants in reproduction, but also explain the impact of antiox...
Journal Of Environmental Science And Health, Part A, Jul 15, 2012
To investigate the effects of lead (Pb) and cadmium (Cd) content on basic motility characteristic... more To investigate the effects of lead (Pb) and cadmium (Cd) content on basic motility characteristics (motility, progressive motility) and selected antioxidant parameters (total antioxidant status - TAS, superoxide dismutase - SOD, albumin - ALB) in the bovine seminal plasma semen samples were collected from breeding bulls and used in the study. Motility analysis was carried out using the Computer Assisted Sperm Analysis (CASA) system. Subsequently, the samples were centrifuged and fractions of seminal plasma were collected. Pb and Cd concentrations were determined by the voltametric method (ASV), antioxidant parameters were analyzed by UV/VIS spectrophotometry using commercial kits. The analysis showed that the average concentrations of the trace elements were 0.57 ± 0.01 μg/mL for Pb and 0.11 ± 0.01 μg/mL for Cd. The correlation analysis revealed that both heavy metals were negatively correlated with motility (r = -0.777; P < 0.001 for Pb and r = -0.786; P < 0.001 for Cd), progressive motility (r = -0.763; P < 0.001 for Pb and r = -0.792; P < 0.001 for Cd), TAS (r = -0.375; p > 0.05 and r = -0.334; P > 0.05, respectively), SOD (r = -0.746; P < 0.001 and r = -0.537; P < 0.05, respectively) as well as with ALB (r = -0.609; P < 0.01 and r = -0.699; P < 0.001, respectively). Moreover the samples were categorized in three quality groups (Excellent, Good, Medium) according to their motility values. The lowest Pb and Cd concentrations but the best antioxidant characteristics were found in samples of excellent quality, medium quality samples were described by the highest Pb and Cd concentration and the worst antioxidant power. This study demonstrates that Pb and Cd are serious toxic elements, which are able to increase the risk of oxidative stress development and a subsequent decrease of semen quality.
The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential e... more The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 μM) of FeSO4.7H2O and compared with the control group (culture medium without FeSO4.7H2O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO4.7H2O, significantly (P < 0.001) increased production of progesterone was observed at the lowest concentration (3.90 μM) of FeSO4.7H2O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 μM of FeSO4.7H2O, which did not elicit cytotoxic action (P > 0.05). Testosterone production was substantially increased at the concentrations ≤ ...
Nonylphenol (NP), an environmental endocrine disruptor, is a final metabolite of nonylphenol etho... more Nonylphenol (NP), an environmental endocrine disruptor, is a final metabolite of nonylphenol ethoxylate (NPE) that is able to interfere with hormonal system of numerous organisms. Estrogens play a central role in female reproduction, but also affect the male reproductive system. In males, stimulate mammalian spermatozoa capacitation, acrosome reaction and fertilizing ability. The aim of this in vitro study was to determine the effect of 17β-estradiol and nonylphenol (NP) on the spermatozoa motility. Specifically, we examined the doseand time-dependent effect of nonylphenol (1, 10, 100 and 200 μg/mL) with the addition 1 μg/mL of 17β-estradiol on the motility and progressive motility of bovine spermatozoa during two time periods (0 h and 6 h). The spermatozoa motility was determined by CASA (Computer Assisted Semen Analyzer) system using the Sperm Vision program. The results showed decreased average values of motility in all experimental groups during 0 h of in vitro cultivation. The ...
Exogenous substances altering the function of the endocrine system and exhibiting adverse health ... more Exogenous substances altering the function of the endocrine system and exhibiting adverse health effects on the organism are defined as endocrine disruptors. Nonylphenol is one of the most abundant alkylphenol ethoxylate derivatives, being detected in food products. Diverse studies have classified nonylphenol as hazardous to the health, especially to male reproduction. This in vitro study aimed to examine the effects of 4-nonylphenol on androstenedione and testosterone production as well as on the viability of Leydig cells of NMRI mice. The cells were cultured for 44 h with addition of 0.04; 0.2; 1.0; 2.5 and 5.0 μg/ml of 4-nonylphenol and compared to the control. Quantification of testosterone and androstenedione directly from aliquots of the medium was performed by enzyme-linked immunosorbent assay. Cell viability was measured by the metabolic activity assay for mitochondrial functional activity. Androstenedione production significantly (P < 0.001) increased with 1.0; 2.5 and 5...
The Journal of Microbiology, Biotechnology and Food Sciences, 2012
To investigate the effects of iron (Fe) and copper (Cu) content on basic motility characteristics... more To investigate the effects of iron (Fe) and copper (Cu) content on basic motility characteristics (motility, progressive motility) and total antioxidant status (TAS) in the bovine seminal plasma semen samples were collected from breeding bulls and used in the study. Motility analysis was carried out using the Computer Assisted Sperm Analysis (CASA) system. Subsequently, the samples were centrifuged and seminal plasma was collected. Fe and Cu concentrations were determined by flame absorption spectrophotometry (FAS), TAS was analyzed by UV/VIS spectrophotometry. The analysis showed that the average concentrations of the trace elements were 39.95±0.07 μg/mL for Fe and 1.55±0.22 μg/mL for Cu. The correlation analysis revealed that both metals were positively correlated with motility (r=0.527; P<0.05 for Fe and r=0.571; P<0.05 for Cu), progressive motility (r=0.496; P<0.05 for Fe and r=0.513; P<0.05 for Cu), as well as TAS (r=0.694; P<0.01 for Cu; r=0.519; P<0.05 for F...
The aim of this study was to assess the dose- and time-dependentin vitroeffects of ferrous sulpha... more The aim of this study was to assess the dose- and time-dependentin vitroeffects of ferrous sulphate (FeSO4.7H2O) on the motility parameters, viability, structural and functional activity of bovine spermatozoa. Spermatozoa motility parameters were determined after exposure to concentrations (3.90, 7.80, 15.60, 31.20, 62.50, 125, 250, 500 and 1000 μM) of FeSO4.7H2O using the SpermVisionTMCASA (Computer Assisted Semen Analyzer) system in different time periods. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, and the Annexin V-Fluos was applied to detect the membrane integrity of spermatozoa. The initial spermatozoa motility showed increased average values at all experimental concentrations compared to the control group (culture medium without FeSO4.7H2O). After 2 h, FeSO4.7H2O stimulated the overall percentage of spermatozoa motility at the concentrations of ≤ 125 μM. However, experimental administration of 250 μM of FeSO4.7H2O ...
Oxidative stress is a state related to an increased cellular damage produced by oxygen and oxygen... more Oxidative stress is a state related to an increased cellular damage produced by oxygen and oxygen derived free radicals known as reactive oxygen species (ROS). It is a serious condition, as ROS and their metabolites attack DNA, lipids and proteins, alter enzymatic systems and cell signalizing pathways producing irreparable alterations, cell death and necrosis. While small amounts of ROS have been shown to be required for some physiological functions, their excessive levels can impair an overall fertilization capacity. This questions have recently attracted the attention of the scientific society, however, research in exploring the role of oxidative stress and antioxidants associated with fertility remains in his initial stages and therefore needs further exploration. This review summarizes the latest consensus on the role of ROS and antioxidants in reproduction. It should not only help to clarify the role of ROS and antioxidants in reproduction, but also explain the impact of antiox...
Journal Of Environmental Science And Health, Part A, Jul 15, 2012
To investigate the effects of lead (Pb) and cadmium (Cd) content on basic motility characteristic... more To investigate the effects of lead (Pb) and cadmium (Cd) content on basic motility characteristics (motility, progressive motility) and selected antioxidant parameters (total antioxidant status - TAS, superoxide dismutase - SOD, albumin - ALB) in the bovine seminal plasma semen samples were collected from breeding bulls and used in the study. Motility analysis was carried out using the Computer Assisted Sperm Analysis (CASA) system. Subsequently, the samples were centrifuged and fractions of seminal plasma were collected. Pb and Cd concentrations were determined by the voltametric method (ASV), antioxidant parameters were analyzed by UV/VIS spectrophotometry using commercial kits. The analysis showed that the average concentrations of the trace elements were 0.57 ± 0.01 μg/mL for Pb and 0.11 ± 0.01 μg/mL for Cd. The correlation analysis revealed that both heavy metals were negatively correlated with motility (r = -0.777; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001 for Pb and r = -0.786; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001 for Cd), progressive motility (r = -0.763; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001 for Pb and r = -0.792; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001 for Cd), TAS (r = -0.375; p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05 and r = -0.334; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0.05, respectively), SOD (r = -0.746; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001 and r = -0.537; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05, respectively) as well as with ALB (r = -0.609; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.01 and r = -0.699; P &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001, respectively). Moreover the samples were categorized in three quality groups (Excellent, Good, Medium) according to their motility values. The lowest Pb and Cd concentrations but the best antioxidant characteristics were found in samples of excellent quality, medium quality samples were described by the highest Pb and Cd concentration and the worst antioxidant power. This study demonstrates that Pb and Cd are serious toxic elements, which are able to increase the risk of oxidative stress development and a subsequent decrease of semen quality.
The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential e... more The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 μM) of FeSO4.7H2O and compared with the control group (culture medium without FeSO4.7H2O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO4.7H2O, significantly (P < 0.001) increased production of progesterone was observed at the lowest concentration (3.90 μM) of FeSO4.7H2O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 μM of FeSO4.7H2O, which did not elicit cytotoxic action (P > 0.05). Testosterone production was substantially increased at the concentrations ≤ ...
Nonylphenol (NP), an environmental endocrine disruptor, is a final metabolite of nonylphenol etho... more Nonylphenol (NP), an environmental endocrine disruptor, is a final metabolite of nonylphenol ethoxylate (NPE) that is able to interfere with hormonal system of numerous organisms. Estrogens play a central role in female reproduction, but also affect the male reproductive system. In males, stimulate mammalian spermatozoa capacitation, acrosome reaction and fertilizing ability. The aim of this in vitro study was to determine the effect of 17β-estradiol and nonylphenol (NP) on the spermatozoa motility. Specifically, we examined the doseand time-dependent effect of nonylphenol (1, 10, 100 and 200 μg/mL) with the addition 1 μg/mL of 17β-estradiol on the motility and progressive motility of bovine spermatozoa during two time periods (0 h and 6 h). The spermatozoa motility was determined by CASA (Computer Assisted Semen Analyzer) system using the Sperm Vision program. The results showed decreased average values of motility in all experimental groups during 0 h of in vitro cultivation. The ...
Exogenous substances altering the function of the endocrine system and exhibiting adverse health ... more Exogenous substances altering the function of the endocrine system and exhibiting adverse health effects on the organism are defined as endocrine disruptors. Nonylphenol is one of the most abundant alkylphenol ethoxylate derivatives, being detected in food products. Diverse studies have classified nonylphenol as hazardous to the health, especially to male reproduction. This in vitro study aimed to examine the effects of 4-nonylphenol on androstenedione and testosterone production as well as on the viability of Leydig cells of NMRI mice. The cells were cultured for 44 h with addition of 0.04; 0.2; 1.0; 2.5 and 5.0 μg/ml of 4-nonylphenol and compared to the control. Quantification of testosterone and androstenedione directly from aliquots of the medium was performed by enzyme-linked immunosorbent assay. Cell viability was measured by the metabolic activity assay for mitochondrial functional activity. Androstenedione production significantly (P < 0.001) increased with 1.0; 2.5 and 5...
The Journal of Microbiology, Biotechnology and Food Sciences, 2012
To investigate the effects of iron (Fe) and copper (Cu) content on basic motility characteristics... more To investigate the effects of iron (Fe) and copper (Cu) content on basic motility characteristics (motility, progressive motility) and total antioxidant status (TAS) in the bovine seminal plasma semen samples were collected from breeding bulls and used in the study. Motility analysis was carried out using the Computer Assisted Sperm Analysis (CASA) system. Subsequently, the samples were centrifuged and seminal plasma was collected. Fe and Cu concentrations were determined by flame absorption spectrophotometry (FAS), TAS was analyzed by UV/VIS spectrophotometry. The analysis showed that the average concentrations of the trace elements were 39.95±0.07 μg/mL for Fe and 1.55±0.22 μg/mL for Cu. The correlation analysis revealed that both metals were positively correlated with motility (r=0.527; P<0.05 for Fe and r=0.571; P<0.05 for Cu), progressive motility (r=0.496; P<0.05 for Fe and r=0.513; P<0.05 for Cu), as well as TAS (r=0.694; P<0.01 for Cu; r=0.519; P<0.05 for F...
The aim of this study was to assess the dose- and time-dependentin vitroeffects of ferrous sulpha... more The aim of this study was to assess the dose- and time-dependentin vitroeffects of ferrous sulphate (FeSO4.7H2O) on the motility parameters, viability, structural and functional activity of bovine spermatozoa. Spermatozoa motility parameters were determined after exposure to concentrations (3.90, 7.80, 15.60, 31.20, 62.50, 125, 250, 500 and 1000 μM) of FeSO4.7H2O using the SpermVisionTMCASA (Computer Assisted Semen Analyzer) system in different time periods. Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, and the Annexin V-Fluos was applied to detect the membrane integrity of spermatozoa. The initial spermatozoa motility showed increased average values at all experimental concentrations compared to the control group (culture medium without FeSO4.7H2O). After 2 h, FeSO4.7H2O stimulated the overall percentage of spermatozoa motility at the concentrations of ≤ 125 μM. However, experimental administration of 250 μM of FeSO4.7H2O ...
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