Sorafenib is an oral multi-kinase receptor inhibitor that targets various signaling pathways. It ... more Sorafenib is an oral multi-kinase receptor inhibitor that targets various signaling pathways. It is used as the first line of treatment in advanced hepatocellular and renal cell carcinomas. Sorafenib was reported to induce cardiotoxicity due to myocyte necrosis. Hesperetin is a naturally occurring flavonoid with antioxidant and anti-inflammatory capabilities. This study investigated the putative protective effect of hesperetin against sorafenib-induced cardiotoxicity in mice through downregulation of NLRP3/TLR4 signaling and inhibition of apoptosis. Twenty-four male Swiss mice were distributed into four groups: untreated control, hesperetin (50 mg/kg/day, orally), sorafenib (100 mg/kg/day, orally), and combination (Hesperetin+Sorafenib). After a three-week treatment period, various biochemical parameters in cardiac tissues were assessed. TNF-α, IL-1β, and IL-6 levels were measured. Moreover, TLR4 and NLRP3 expressions were evaluated using Western blot analysis. Histopathological exa...
Chemotherapeutic-related toxicity exacerbates the increasing death rate among cancer patients, ne... more Chemotherapeutic-related toxicity exacerbates the increasing death rate among cancer patients, necessitating greater efforts to find a speedy solution. An in vivo assessment of the protective effect of the C. macrocarpa leaves polar fraction of hydromethanolic extract against doxorubicin (Dox)-induced neurotoxicity was performed. Intriguingly, this fraction ameliorated Dox-induced cognitive dysfunction; reduced serum ROS and brain TNF-α levels, upregulated the brain nerve growth factor (NGF) levels, markedly reduced caspase-3 immunoexpression, and restored the histological architecture of the brain hippocampus. The in vivo study results were corroborated with a UPLC-ESI-MS/MS profiling that revealed the presence of a high percentage of the plant polyphenolics. Molecular modeling of several identified molecules in this fraction demonstrated a strong binding affinity of flavan-3-ol derivatives with TACE enzymes, in agreement with the experimental in vivo neuroprotective activity. In c...
This study was conducted to explore the potential cardioprotective and anti-depressive effects of... more This study was conducted to explore the potential cardioprotective and anti-depressive effects of dichloromethane fractions of Morus macroura leaves and stem branches on post-myocardial infarction depression induced by isoprenaline in rats in relation to their metabolites.
ETHNOPHARMACOLOGICAL RELEVANCE Ashwagandha (ASH) is one of the medicinal plants that is used in t... more ETHNOPHARMACOLOGICAL RELEVANCE Ashwagandha (ASH) is one of the medicinal plants that is used in traditional Indian, Ayurvedic and Unani medicines for their broad range of pharmacological activities including tonic, aphrodisiac, energy stimulant, and counteracting chronic fatigue. Besides, it is used in the treatment of nervous exhaustion, memory related conditions, insomnia, as well as improving learning ability and memory capacity. ASH is preclinically proven to be efficient in hepatoprotection and enhancing cognitive impairment, however, its beneficial effects against hepatic encephalopathy (HE) is still unclear. Therefore, this study aimed at investigating the protective effects of ASH root extract against thioacetamide (TAA)-induced HE and delineate the underlying behavioural and pharmacological mechanisms. MATERIALS AND METHODS ASH metabolites were identified using UPLC-HRMS. Rats were pretreated with ASH (200 and 400 mg/kg) for 29 days and administrated TAA (i.p, 350 mg/kg) in a single dose. Then, behavioral (open field test, Y-maze, modified elevated plus maze and novel object recognition test), and biochemical (ammonia and hepatic toxicity indices) assessments, as well as oxidative stress markers (MDA and GSH) were evaluated. The hepatic and brain levels of glutamine synthetase (GS), nuclear factor erythroid 2-related factor 2 (Nfr2), heme-oxygenase (HO)-1, inducible nitric oxide synthase (iNOS) were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of p 38/ERK ½ were determined using real-time polymerase chain reaction (PCR). Moreover, histopathological investigations and immunohistochemical (NF-κB and TNF-α immunohistochemical expressions) examinations were performed. RESULTS Metabolite profiling of ASH revealed more than 45 identified metabolites including phenolic acids, flavonoids and steroidal lactone triterpenoids. Compared to TAA-intoxicated group; ASH improved locomotor and cognitive deficits, serum hepatotoxicity indices and ammonia levels, besides brain and hepatic histopathological alterations. ASH reduced hepatic and brain levels of MDA, GSH, GS, iNOS, Nfr2 and HO-1. ASH downregulated p38 and ERK ½ mRNA expressions, NF-κB and TNF-α immunohistochemical expressions in brain and hepatic tissues. CONCLUSIONS Accordingly, our results provided insights into the promising hepato- and neuroprotective effects of ASH, with superiority to 400 mg/kg ASH, to ameliorate HE with its sequential hyperammonemia and liver/brain injuries. This could be attributed to the recorded increase in spontaneous alternation % and recognition index, antioxidant and anti-inflammatory activities as well as downregulation of Nrf2 and MAPK signaling pathways.
This study was performed at Giza station, Cotton Res. Inst., Agric. Res. Center, Egypt, during 20... more This study was performed at Giza station, Cotton Res. Inst., Agric. Res. Center, Egypt, during 2012 and 2013 seasons. To decrease the chemical pollution of soil and underground water and study the effect of applying clay soil with rice straw as organic wastes, and the inoculation with some bacterial strains as biofertilizer (PGPR) (Trichoderma reesei, Trichoderma viride, Pseudomonas putida, Bacillus polymexa, Bacillus megaterium). Together with or without rice straw using eco-friendly crop such as, colored cotton because of the lack need for chemicals during bleaching and dyeing processes. As well as, study their effect on soil chemical and biological properties and their impact on the green and brown colored cotton fiber quality comparing to the chemical fertilizers, and cotton yield and some yield components and cotton fiber properties comparing to the control. Green cotton (mutation from G.89), brown cotton (mutation from G.83) and G.86 as white cotton as control. The most import...
The current study was carried out to investigate the role of unpredictable chronic mild stress as... more The current study was carried out to investigate the role of unpredictable chronic mild stress as a model of depression on the physical and behavioral parameters of male Swiss mice. TwentyFive adult male Swiss albino mice were housed in either control group or unpredictable chronic mild stress (UCMS) group that was exposed to different physical stressors for 8 weeks. Body weight and body coat were assessed. Sucrose consumption test, reward maze test, elevated plus maze and forced swim test were conducted. UCMS mice displayed a reduction in body weight, and decreased sucrose consumption, while exhibited an increase in the latency to pass 1 st gate of the reward maze. UCMS mice also showed a tendency of being in the closed arm of the elevated plus maze for a longer duration compared to mice in the control group, a tendency of being immobile in forced swim test. In conclusion, physical changes in conjunction with the significant behavioral changes strongly support the efficacy of UCMS ...
Salt-tolerant of plant growth promoting rhizobacteria (PGPR) Azotobacterchrocooccum, Azospirillum... more Salt-tolerant of plant growth promoting rhizobacteria (PGPR) Azotobacterchrocooccum, Azospirillumbrasilense and cyanobacteria Anabaenaoryzaeused to reduce the impact of salinity on plant growth and improved productivity. Laboratory experiments proved that all used bacteria excrete plant growth promoting substances such as exopolysaccharide (EPS), indole acetic acid (IAA), Catalase and Hydrogen peroxide (H2O2) with excellence for azospirillum in (EPS)and (IAA), while Azotobacterchrocooccum and cyanobacteria were superior in Catalase and Hydrogen peroxide (H2O2) with salinity that led to an increasing inRice plant traits at harvest of Rice plant as a result of inoculation with three bacteria that increasing all macronutrients and micronutrients in both straw and grains of Rice harvest over the control beside decreasing of pH and EC in the soil after planting season. These confirm that inoculation by PGPR made as growth regulators considerably alleviate the soil salinity.
Greater understanding of the efficiency of nanoparticles will assist future research related to m... more Greater understanding of the efficiency of nanoparticles will assist future research related to male reproductive performance. The current study was performed to assess the potency of selenium nanoparticles (SeNPs) in alleviating deltamethrin (DLM)-induced detrimental effects on sperm characteristics, oxidative status, sexual behavior, and the histological structure of the testes and epididymis in male rats. Thirty-two male Wister rats were divided into four groups according to treatment received orally by gavage 3 times/week for 60 days; control, DLM (0.6 mg/kg bwt), SeNPs (0.5 mg/kg bwt), and DLM-SeNPs groups. DLM caused a significant reduction in sperm count, motility, and viability percent, as well as in body weight and serum testosterone level, blood total antioxidant capacity (TAC), and glutathione peroxidase (GPx) activity. The DLM-treated group showed a significant increase in blood malondialdehyde (MDA) concentration and sperm abnormalities (%), as well as a significant reduction in sexual activity, manifested as an increase in mount, intromission, or ejaculation latency and a reduction in mount or intromission frequency. These toxic effects were confirmed by histological alterations, represented by a significant reduction in the diameter of the seminiferous tubules and spermatogenesis. Conversely, treatment with SeNPs improved DLM-induced negative effects on sperm characteristics, testosterone, and antioxidant biomarkers, as well as behavioral and histopathological alterations. The SeNPs treated group showed improved semen parameters, antioxidant status, and sexual performance. In conclusion, SeNPs may represent an effective treatment for reducing the detrimental effects of DLM on male fertility, and lead to enhanced male reproductive performance.
The current study was conducted to investigate the effect of unpredictable chronic mild stress as... more The current study was conducted to investigate the effect of unpredictable chronic mild stress as a model of depression on the physiological and neurochemical parameters of male swiss mice. TwentyFive adult male Swiss albino mice were housed in either control group or unpredictable chronic mild stress (UCMS) group that was exposed to different physical stressors for 8 weeks. Plasma corticosterone and brain stem levels of dopamine and serotonin (5-OH) were measured. UCMS mice displayed an increase in the plasma corticosterone concentration and lower levels of both brain stem dopamine and 5 OHT compared to controls. In conclusion, the presence of physiological changes along with the significant neurobiological changes in the brain strongly support the efficacy of UCMS as a model of depression in the human being.
Sorafenib is an oral multi-kinase receptor inhibitor that targets various signaling pathways. It ... more Sorafenib is an oral multi-kinase receptor inhibitor that targets various signaling pathways. It is used as the first line of treatment in advanced hepatocellular and renal cell carcinomas. Sorafenib was reported to induce cardiotoxicity due to myocyte necrosis. Hesperetin is a naturally occurring flavonoid with antioxidant and anti-inflammatory capabilities. This study investigated the putative protective effect of hesperetin against sorafenib-induced cardiotoxicity in mice through downregulation of NLRP3/TLR4 signaling and inhibition of apoptosis. Twenty-four male Swiss mice were distributed into four groups: untreated control, hesperetin (50 mg/kg/day, orally), sorafenib (100 mg/kg/day, orally), and combination (Hesperetin+Sorafenib). After a three-week treatment period, various biochemical parameters in cardiac tissues were assessed. TNF-α, IL-1β, and IL-6 levels were measured. Moreover, TLR4 and NLRP3 expressions were evaluated using Western blot analysis. Histopathological exa...
Chemotherapeutic-related toxicity exacerbates the increasing death rate among cancer patients, ne... more Chemotherapeutic-related toxicity exacerbates the increasing death rate among cancer patients, necessitating greater efforts to find a speedy solution. An in vivo assessment of the protective effect of the C. macrocarpa leaves polar fraction of hydromethanolic extract against doxorubicin (Dox)-induced neurotoxicity was performed. Intriguingly, this fraction ameliorated Dox-induced cognitive dysfunction; reduced serum ROS and brain TNF-α levels, upregulated the brain nerve growth factor (NGF) levels, markedly reduced caspase-3 immunoexpression, and restored the histological architecture of the brain hippocampus. The in vivo study results were corroborated with a UPLC-ESI-MS/MS profiling that revealed the presence of a high percentage of the plant polyphenolics. Molecular modeling of several identified molecules in this fraction demonstrated a strong binding affinity of flavan-3-ol derivatives with TACE enzymes, in agreement with the experimental in vivo neuroprotective activity. In c...
This study was conducted to explore the potential cardioprotective and anti-depressive effects of... more This study was conducted to explore the potential cardioprotective and anti-depressive effects of dichloromethane fractions of Morus macroura leaves and stem branches on post-myocardial infarction depression induced by isoprenaline in rats in relation to their metabolites.
ETHNOPHARMACOLOGICAL RELEVANCE Ashwagandha (ASH) is one of the medicinal plants that is used in t... more ETHNOPHARMACOLOGICAL RELEVANCE Ashwagandha (ASH) is one of the medicinal plants that is used in traditional Indian, Ayurvedic and Unani medicines for their broad range of pharmacological activities including tonic, aphrodisiac, energy stimulant, and counteracting chronic fatigue. Besides, it is used in the treatment of nervous exhaustion, memory related conditions, insomnia, as well as improving learning ability and memory capacity. ASH is preclinically proven to be efficient in hepatoprotection and enhancing cognitive impairment, however, its beneficial effects against hepatic encephalopathy (HE) is still unclear. Therefore, this study aimed at investigating the protective effects of ASH root extract against thioacetamide (TAA)-induced HE and delineate the underlying behavioural and pharmacological mechanisms. MATERIALS AND METHODS ASH metabolites were identified using UPLC-HRMS. Rats were pretreated with ASH (200 and 400 mg/kg) for 29 days and administrated TAA (i.p, 350 mg/kg) in a single dose. Then, behavioral (open field test, Y-maze, modified elevated plus maze and novel object recognition test), and biochemical (ammonia and hepatic toxicity indices) assessments, as well as oxidative stress markers (MDA and GSH) were evaluated. The hepatic and brain levels of glutamine synthetase (GS), nuclear factor erythroid 2-related factor 2 (Nfr2), heme-oxygenase (HO)-1, inducible nitric oxide synthase (iNOS) were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of p 38/ERK ½ were determined using real-time polymerase chain reaction (PCR). Moreover, histopathological investigations and immunohistochemical (NF-κB and TNF-α immunohistochemical expressions) examinations were performed. RESULTS Metabolite profiling of ASH revealed more than 45 identified metabolites including phenolic acids, flavonoids and steroidal lactone triterpenoids. Compared to TAA-intoxicated group; ASH improved locomotor and cognitive deficits, serum hepatotoxicity indices and ammonia levels, besides brain and hepatic histopathological alterations. ASH reduced hepatic and brain levels of MDA, GSH, GS, iNOS, Nfr2 and HO-1. ASH downregulated p38 and ERK ½ mRNA expressions, NF-κB and TNF-α immunohistochemical expressions in brain and hepatic tissues. CONCLUSIONS Accordingly, our results provided insights into the promising hepato- and neuroprotective effects of ASH, with superiority to 400 mg/kg ASH, to ameliorate HE with its sequential hyperammonemia and liver/brain injuries. This could be attributed to the recorded increase in spontaneous alternation % and recognition index, antioxidant and anti-inflammatory activities as well as downregulation of Nrf2 and MAPK signaling pathways.
This study was performed at Giza station, Cotton Res. Inst., Agric. Res. Center, Egypt, during 20... more This study was performed at Giza station, Cotton Res. Inst., Agric. Res. Center, Egypt, during 2012 and 2013 seasons. To decrease the chemical pollution of soil and underground water and study the effect of applying clay soil with rice straw as organic wastes, and the inoculation with some bacterial strains as biofertilizer (PGPR) (Trichoderma reesei, Trichoderma viride, Pseudomonas putida, Bacillus polymexa, Bacillus megaterium). Together with or without rice straw using eco-friendly crop such as, colored cotton because of the lack need for chemicals during bleaching and dyeing processes. As well as, study their effect on soil chemical and biological properties and their impact on the green and brown colored cotton fiber quality comparing to the chemical fertilizers, and cotton yield and some yield components and cotton fiber properties comparing to the control. Green cotton (mutation from G.89), brown cotton (mutation from G.83) and G.86 as white cotton as control. The most import...
The current study was carried out to investigate the role of unpredictable chronic mild stress as... more The current study was carried out to investigate the role of unpredictable chronic mild stress as a model of depression on the physical and behavioral parameters of male Swiss mice. TwentyFive adult male Swiss albino mice were housed in either control group or unpredictable chronic mild stress (UCMS) group that was exposed to different physical stressors for 8 weeks. Body weight and body coat were assessed. Sucrose consumption test, reward maze test, elevated plus maze and forced swim test were conducted. UCMS mice displayed a reduction in body weight, and decreased sucrose consumption, while exhibited an increase in the latency to pass 1 st gate of the reward maze. UCMS mice also showed a tendency of being in the closed arm of the elevated plus maze for a longer duration compared to mice in the control group, a tendency of being immobile in forced swim test. In conclusion, physical changes in conjunction with the significant behavioral changes strongly support the efficacy of UCMS ...
Salt-tolerant of plant growth promoting rhizobacteria (PGPR) Azotobacterchrocooccum, Azospirillum... more Salt-tolerant of plant growth promoting rhizobacteria (PGPR) Azotobacterchrocooccum, Azospirillumbrasilense and cyanobacteria Anabaenaoryzaeused to reduce the impact of salinity on plant growth and improved productivity. Laboratory experiments proved that all used bacteria excrete plant growth promoting substances such as exopolysaccharide (EPS), indole acetic acid (IAA), Catalase and Hydrogen peroxide (H2O2) with excellence for azospirillum in (EPS)and (IAA), while Azotobacterchrocooccum and cyanobacteria were superior in Catalase and Hydrogen peroxide (H2O2) with salinity that led to an increasing inRice plant traits at harvest of Rice plant as a result of inoculation with three bacteria that increasing all macronutrients and micronutrients in both straw and grains of Rice harvest over the control beside decreasing of pH and EC in the soil after planting season. These confirm that inoculation by PGPR made as growth regulators considerably alleviate the soil salinity.
Greater understanding of the efficiency of nanoparticles will assist future research related to m... more Greater understanding of the efficiency of nanoparticles will assist future research related to male reproductive performance. The current study was performed to assess the potency of selenium nanoparticles (SeNPs) in alleviating deltamethrin (DLM)-induced detrimental effects on sperm characteristics, oxidative status, sexual behavior, and the histological structure of the testes and epididymis in male rats. Thirty-two male Wister rats were divided into four groups according to treatment received orally by gavage 3 times/week for 60 days; control, DLM (0.6 mg/kg bwt), SeNPs (0.5 mg/kg bwt), and DLM-SeNPs groups. DLM caused a significant reduction in sperm count, motility, and viability percent, as well as in body weight and serum testosterone level, blood total antioxidant capacity (TAC), and glutathione peroxidase (GPx) activity. The DLM-treated group showed a significant increase in blood malondialdehyde (MDA) concentration and sperm abnormalities (%), as well as a significant reduction in sexual activity, manifested as an increase in mount, intromission, or ejaculation latency and a reduction in mount or intromission frequency. These toxic effects were confirmed by histological alterations, represented by a significant reduction in the diameter of the seminiferous tubules and spermatogenesis. Conversely, treatment with SeNPs improved DLM-induced negative effects on sperm characteristics, testosterone, and antioxidant biomarkers, as well as behavioral and histopathological alterations. The SeNPs treated group showed improved semen parameters, antioxidant status, and sexual performance. In conclusion, SeNPs may represent an effective treatment for reducing the detrimental effects of DLM on male fertility, and lead to enhanced male reproductive performance.
The current study was conducted to investigate the effect of unpredictable chronic mild stress as... more The current study was conducted to investigate the effect of unpredictable chronic mild stress as a model of depression on the physiological and neurochemical parameters of male swiss mice. TwentyFive adult male Swiss albino mice were housed in either control group or unpredictable chronic mild stress (UCMS) group that was exposed to different physical stressors for 8 weeks. Plasma corticosterone and brain stem levels of dopamine and serotonin (5-OH) were measured. UCMS mice displayed an increase in the plasma corticosterone concentration and lower levels of both brain stem dopamine and 5 OHT compared to controls. In conclusion, the presence of physiological changes along with the significant neurobiological changes in the brain strongly support the efficacy of UCMS as a model of depression in the human being.
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