Background The KNOTTED1-LIKE HOMEOBOX PROTEIN1 ( KD1 ) gene is highly expressed in flower and lea... more Background The KNOTTED1-LIKE HOMEOBOX PROTEIN1 ( KD1 ) gene is highly expressed in flower and leaf abscission zones (AZs). RNA-antisense silencing of KD1 was shown to delay tomato pedicel and petiole abscission, induced by flower or leaf removal, respectively. KD1 was found to regulate flower pedicel abscission via alteration of auxin gradient through the flower AZ (FAZ), and disruption of the auxin response at the early stages of the abscission process. The present work was aimed to further understand how KD1 regulates signaling factors and regulatory genes involved in the delay of pedicel abscission using the silenced KD1 lines. For this purpose we performed a large scale transcriptome profiling of the FAZ at various time points after flower removal, using a customized AZ-specific microarray. Results The results highlighted a differential expression of regulatory genes in the FAZ of KD1 -silenced plants compared to the wild type (WT). These genes were controlled by KD1 before and ...
The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle l... more The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of prote...
The Tomato Hybrid Proline-rich Protein () gene was specifically expressed in the tomato () flower... more The Tomato Hybrid Proline-rich Protein () gene was specifically expressed in the tomato () flower abscission zone (FAZ), and its stable antisense silencing under the control of an abscission zone (AZ)-specific promoter, , significantly inhibited tomato pedicel abscission following flower removal. For understanding the THyPRP role in regulating pedicel abscission, a transcriptomic analysis of the FAZ of -silenced plants was performed, using a newly developed AZ-specific tomato microarray chip. Decreased expression of in the silenced plants was already observed before abscission induction, resulting in FAZ-specific altered gene expression of transcription factors, epigenetic modifiers, post-translational regulators, and transporters. Our data demonstrate that the effect of silencing on pedicel abscission was not mediated by its effect on auxin balance, but by decreased ethylene biosynthesis and response. Additionally, silencing revealed new players, which were demonstrated for the fir...
The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle l... more The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of prote...
Background The KNOTTED1-LIKE HOMEOBOX PROTEIN1 ( KD1 ) gene is highly expressed in flower and lea... more Background The KNOTTED1-LIKE HOMEOBOX PROTEIN1 ( KD1 ) gene is highly expressed in flower and leaf abscission zones (AZs). RNA-antisense silencing of KD1 was shown to delay tomato pedicel and petiole abscission, induced by flower or leaf removal, respectively. KD1 was found to regulate flower pedicel abscission via alteration of auxin gradient through the flower AZ (FAZ), and disruption of the auxin response at the early stages of the abscission process. The present work was aimed to further understand how KD1 regulates signaling factors and regulatory genes involved in the delay of pedicel abscission using the silenced KD1 lines. For this purpose we performed a large scale transcriptome profiling of the FAZ at various time points after flower removal, using a customized AZ-specific microarray. Results The results highlighted a differential expression of regulatory genes in the FAZ of KD1 -silenced plants compared to the wild type (WT). These genes were controlled by KD1 before and ...
The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle l... more The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of prote...
The Tomato Hybrid Proline-rich Protein () gene was specifically expressed in the tomato () flower... more The Tomato Hybrid Proline-rich Protein () gene was specifically expressed in the tomato () flower abscission zone (FAZ), and its stable antisense silencing under the control of an abscission zone (AZ)-specific promoter, , significantly inhibited tomato pedicel abscission following flower removal. For understanding the THyPRP role in regulating pedicel abscission, a transcriptomic analysis of the FAZ of -silenced plants was performed, using a newly developed AZ-specific tomato microarray chip. Decreased expression of in the silenced plants was already observed before abscission induction, resulting in FAZ-specific altered gene expression of transcription factors, epigenetic modifiers, post-translational regulators, and transporters. Our data demonstrate that the effect of silencing on pedicel abscission was not mediated by its effect on auxin balance, but by decreased ethylene biosynthesis and response. Additionally, silencing revealed new players, which were demonstrated for the fir...
The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle l... more The abscission process occurs in a specific abscission zone (AZ) as a consequence of the middle lamella dissolution, cell wall degradation, and formation of a defense layer. The proteins and metabolites related to these processes are secreted by vesicle trafficking through the plasma membrane to the cell wall and middle lamella of the separating cells in the AZ. We investigated this process, since the regulation of vesicle trafficking in abscission systems is poorly understood. The data obtained describe, for the first time, the kinetics of the upregulated expression of genes encoding the components involved in vesicle trafficking, occurring specifically in the tomato (Solanum lycopersicum) flower AZ (FAZ) during pedicel abscission induced by flower removal. The genes encoding vesicle trafficking components included soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), SNARE regulators, and small GTPases. Our results clearly show how the processes of prote...
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Papers by joseph Riov