I am interested to work with Plant growth-promoting microbes which can also inhibit the growth of phytopathogens. I have prior experience working with Pseudomonas fluorescence which inhibits the growth of Fungal phytopathogens which were capable of degrading cellulose by producing cellulase enzyme. I believe using chemically cellulose from discarded sources such as waste papers, we can support the growth of Pseudomonas fluorescence industrially.
International Journal of Scientific Research in Science and Technology
Hospitalizations due to urinary tract infections are among the most commonly observed condition t... more Hospitalizations due to urinary tract infections are among the most commonly observed condition throughout the world. The study conducted among a total of 200 positive urine samples comprising male and female patients of different age showed E. coli (120), Klebsiella spp. (56) and Proteus spp. (24). Based on antibiotic susceptibility testing, It was revealed that E. coli showed lowest resistance to cefotaxime (35%) and highest resistance to levofloxacin (60%), whereas, Klebsiella spp. showed resistance to ceftriaxone (30.59%) and amoxicillin (88.33%). Likewise, Proteus spp. observed to have 29.16% (azithromycin) and 70.83% (ciprofloxacin) resistance. Cefotaxime was found to be the best drug for the treatment of E. coli related infections with 66.67% sensitivity. Ceftriaxone (69.41%) sensitive) was also found to be the best drug for the treatment of Klebsiella spp., whereas Proteus spp. could be effectively treated by Azithromycin (70.83% sensitive). Thirty-six (36) Multi drug resist...
Urinary tract infection (UTI) is the leading cause of death among patients and majority of people... more Urinary tract infection (UTI) is the leading cause of death among patients and majority of people visiting the hospital worldwide. In the current quantitative study, urine samples were obtained using sterile method and 100 isolates, including 60 Escherichia coli, 28 Klebsiella sp., and 12 Proteus sp., were investigated in a laboratory. Current study showed that E. coli 60/100 (60%) was the most common cause of UTI followed by Klebsiella sp. 28/100 (28%) and Proteus sp. 12/100 (12%). Cefotaxime (86.67% sensitive) was found to be the best drug for treating infections with E. coli, ceftazidime (100% sensitive) for infections with Klebsiella sp., and imipenem and levofloxacin (100% sensitive) for infections with Proteus sp. Overall, 51% of the isolated strains showed high multidrug resistance (MDR). Because of the alarming increase in extensively drug-resistant and MDR uropathogens, which is a concern in public health, the rational use of antimicrobial therapy should be implemented.
Thirteen presumptive thermo-tolerant Lactobacilli isolates were isolated from the small intestine... more Thirteen presumptive thermo-tolerant Lactobacilli isolates were isolated from the small intestines of domestic chickens (Gallus domessticus) and were tested against various commercial antibiotics used in chicken feed to test antibiotic resistance of Lactobacillus spp. Isolates were identified by generating dendogram based on biochemical profiling results which was confirmed using PCR reaction. Bacteriocin produced by Lactobacillus sp. was optimized at different physicochemical factors which showed satisfactory zone of inhibition against pathogenic test organisms, both with and without purification. Four out of thirteen confirmed Lactobacillus sp. isolates showed resistance against Ciprofloxacin and one isolate showed resistance against erythromycin. A dendrogram was generated using biotyping where the isolates exhibited similarity to different species situated at same clusters and PCR assay confirmed isolates as Lactobacillus sp. upto genus level. Lactobacillus population will not d...
Natural fibres are getting importance for their sustainable development in their uses in mitigati... more Natural fibres are getting importance for their sustainable development in their uses in mitigation of climate change and ecological balance. A fibre extraction retting method is formulated and proximate chemical composition and various physical properties such as tensile strength, elongation, diameter along with fibre, cellulose, lignin and ash content were determined. This preliminary observation indicates its potential to be used as a source of fibre for textile and non-textile uses such as woven, nonwoven, composite, blanded and a good source of α-cellulose, microcrystalline cellulose, nano-cellulose and lignin-based products. Key word: Ecology, Retting, Sansevieria trifasciata L., Microcrystal cellulose and lignin.
Aims: This study aims to evaluate the microbiological safety in street foods available in Dhaka c... more Aims: This study aims to evaluate the microbiological safety in street foods available in Dhaka city, Bangladesh. Study Design: Seven categories of street foods were collected aseptically in triplicates from 10 locations of the most populous areas of Dhaka city, transported to the microbiology laboratory of Center of Excellence, Primeasia University, Dhaka. The samples were analyzed for microbiological quality. Place and Duration of Study: The study was carried out in Dhaka city, Bangladesh, between November 2015 to March 2017. The microbiological analysis was done at the microbiology laboratory of the Center for Excellence Laboratory (CEL), Department of Microbiology, Primeasia University, Dhaka-1213, Bangladesh. Methodology: Ninety street food samples belonging to 7 different categories were collected aseptically from ten different places in Dhaka. All samples were tested according to the standard food analysis methods. Total viable count (TVC), total coliform count (TCC), total S...
International Journal of Scientific Research in Science and Technology
Hospitalizations due to urinary tract infections are among the most commonly observed condition t... more Hospitalizations due to urinary tract infections are among the most commonly observed condition throughout the world. The study conducted among a total of 200 positive urine samples comprising male and female patients of different age showed E. coli (120), Klebsiella spp. (56) and Proteus spp. (24). Based on antibiotic susceptibility testing, It was revealed that E. coli showed lowest resistance to cefotaxime (35%) and highest resistance to levofloxacin (60%), whereas, Klebsiella spp. showed resistance to ceftriaxone (30.59%) and amoxicillin (88.33%). Likewise, Proteus spp. observed to have 29.16% (azithromycin) and 70.83% (ciprofloxacin) resistance. Cefotaxime was found to be the best drug for the treatment of E. coli related infections with 66.67% sensitivity. Ceftriaxone (69.41%) sensitive) was also found to be the best drug for the treatment of Klebsiella spp., whereas Proteus spp. could be effectively treated by Azithromycin (70.83% sensitive). Thirty-six (36) Multi drug resist...
Urinary tract infection (UTI) is the leading cause of death among patients and majority of people... more Urinary tract infection (UTI) is the leading cause of death among patients and majority of people visiting the hospital worldwide. In the current quantitative study, urine samples were obtained using sterile method and 100 isolates, including 60 Escherichia coli, 28 Klebsiella sp., and 12 Proteus sp., were investigated in a laboratory. Current study showed that E. coli 60/100 (60%) was the most common cause of UTI followed by Klebsiella sp. 28/100 (28%) and Proteus sp. 12/100 (12%). Cefotaxime (86.67% sensitive) was found to be the best drug for treating infections with E. coli, ceftazidime (100% sensitive) for infections with Klebsiella sp., and imipenem and levofloxacin (100% sensitive) for infections with Proteus sp. Overall, 51% of the isolated strains showed high multidrug resistance (MDR). Because of the alarming increase in extensively drug-resistant and MDR uropathogens, which is a concern in public health, the rational use of antimicrobial therapy should be implemented.
Thirteen presumptive thermo-tolerant Lactobacilli isolates were isolated from the small intestine... more Thirteen presumptive thermo-tolerant Lactobacilli isolates were isolated from the small intestines of domestic chickens (Gallus domessticus) and were tested against various commercial antibiotics used in chicken feed to test antibiotic resistance of Lactobacillus spp. Isolates were identified by generating dendogram based on biochemical profiling results which was confirmed using PCR reaction. Bacteriocin produced by Lactobacillus sp. was optimized at different physicochemical factors which showed satisfactory zone of inhibition against pathogenic test organisms, both with and without purification. Four out of thirteen confirmed Lactobacillus sp. isolates showed resistance against Ciprofloxacin and one isolate showed resistance against erythromycin. A dendrogram was generated using biotyping where the isolates exhibited similarity to different species situated at same clusters and PCR assay confirmed isolates as Lactobacillus sp. upto genus level. Lactobacillus population will not d...
Natural fibres are getting importance for their sustainable development in their uses in mitigati... more Natural fibres are getting importance for their sustainable development in their uses in mitigation of climate change and ecological balance. A fibre extraction retting method is formulated and proximate chemical composition and various physical properties such as tensile strength, elongation, diameter along with fibre, cellulose, lignin and ash content were determined. This preliminary observation indicates its potential to be used as a source of fibre for textile and non-textile uses such as woven, nonwoven, composite, blanded and a good source of α-cellulose, microcrystalline cellulose, nano-cellulose and lignin-based products. Key word: Ecology, Retting, Sansevieria trifasciata L., Microcrystal cellulose and lignin.
Aims: This study aims to evaluate the microbiological safety in street foods available in Dhaka c... more Aims: This study aims to evaluate the microbiological safety in street foods available in Dhaka city, Bangladesh. Study Design: Seven categories of street foods were collected aseptically in triplicates from 10 locations of the most populous areas of Dhaka city, transported to the microbiology laboratory of Center of Excellence, Primeasia University, Dhaka. The samples were analyzed for microbiological quality. Place and Duration of Study: The study was carried out in Dhaka city, Bangladesh, between November 2015 to March 2017. The microbiological analysis was done at the microbiology laboratory of the Center for Excellence Laboratory (CEL), Department of Microbiology, Primeasia University, Dhaka-1213, Bangladesh. Methodology: Ninety street food samples belonging to 7 different categories were collected aseptically from ten different places in Dhaka. All samples were tested according to the standard food analysis methods. Total viable count (TVC), total coliform count (TCC), total S...
Chapter - 9 Prevalence, Characterization and Antibiogram Profiling of Fresh Leafy, Salad Vegetables and Rotten Fruits from Different Areas of Dhaka City, Bangladesh, 2022
This study was conducted to determine the microbiological quality of
fresh raw and unwa... more This study was conducted to determine the microbiological quality of fresh raw and unwashed leafy and salad vegetables (red amaranth, spinach, carrot, radish, tomato, and cucumber), different fruits like-Sofeda, Pineapple, Grape, Banana, Apple, Orange, Guava, papaya, Jujube and Star fruit in Dhaka, Bangladesh, where Salmonella spp., Acinetobacter spp., Klebsiella spp., Vibrio cholerae, V. parahaemolyticus, Staphylococcus aureus and E. coli were bacteria and two strains of pathogenic fungi-Aspergillus niger and A. flavus were isolated. In betel leaves, both isolated fungi were showed their highest percentage of frequency (A. niger-66.66% and A. flavus-46.66%) where A. niger showed more pathogenic than A. flavus. The result of the pathogenicity test indicates that all the isolated fungi were pathogenic to their respective samples, except orange. The two species of Aspergillus spp. were found to be associated as the predominant fungi with the rotten fruits, vegetables and betel leaves. In case of fresh leafy and salad vegetables, the total microbial load ranged from 8x10 7 to 1.70x10 8 having 7 different organisms where the most predominant organism was Vibrio sp. (23%) followed by Klebsiella sp. (20%), Acinetobacter sp. (19%), Pseudomonas sp. (19%), Salmonella sp. (8%), Moraxella sp. (8%) and Escherichia coli (3%) and 11% of the Vibrio sp. isolates were V. cholerae, found from 4 samples, but no presence of V. cholerae was observed in the tomato samples. E. coli was observed only in Carrot sample. Commercial antibiotic discs were used for antibiogram by Kirby-Bauer disc diffusion method on Mueller-Hinton agar medium. In case of antibiogram profiling of rotten fruits, majority exhibited resistance against Erythromycin, Vancomycin and Amoxycillin and showing sensitivity against Ciprofloxacin and Ceftriaxone. It was observed in the current study that 100% isolates were resistant against Erythromycin, followed by Amoxycillin 90.63% and Vancomycin 86.25%, where only 35.27% isolates were resistant against Ciprofloxacin. In case of sensitivity 64.73% isolates were sensitive against Ciprofloxacin followed by Ceftriaxone 66.25%. on the contrary, in case of fresh leafy and salad vegetables, the isolated organisms were tested against antibiotics among which Imipenem showed the highest sensitivity (86%), followed by Ceftriaxone (100%), Nitrofurantoin (94%), Erythromycin (89%) and Amoxicillin (83%) had the highest resistance against the isolated organisms; however most of the isolates showed a multi-drug resistance pattern and they were resistant to at least four drugs.
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fresh raw and unwashed leafy and salad vegetables (red amaranth, spinach, carrot, radish, tomato, and cucumber), different fruits like-Sofeda, Pineapple, Grape, Banana, Apple, Orange, Guava, papaya, Jujube and Star fruit in Dhaka, Bangladesh, where Salmonella spp., Acinetobacter spp., Klebsiella spp., Vibrio cholerae, V. parahaemolyticus, Staphylococcus aureus and E. coli were bacteria and two strains of pathogenic fungi-Aspergillus niger and A. flavus were isolated. In betel leaves, both isolated fungi were showed their highest percentage of frequency (A. niger-66.66% and A. flavus-46.66%) where A. niger showed more pathogenic than A. flavus. The result of the
pathogenicity test indicates that all the isolated fungi were pathogenic to their respective samples, except orange. The two species of Aspergillus spp. were found to be associated as the predominant fungi with the rotten fruits, vegetables and betel leaves. In case of fresh leafy and salad vegetables, the total microbial load ranged from 8x10 7 to 1.70x10 8 having 7 different organisms where the most predominant organism was Vibrio sp. (23%) followed by Klebsiella sp. (20%), Acinetobacter sp. (19%), Pseudomonas sp. (19%), Salmonella sp. (8%), Moraxella sp. (8%) and Escherichia coli (3%) and 11% of the Vibrio sp. isolates were V. cholerae, found from 4 samples, but no presence of V. cholerae was observed in the tomato samples. E. coli was observed only in Carrot sample. Commercial antibiotic discs were used for antibiogram by Kirby-Bauer disc diffusion method on Mueller-Hinton agar medium. In case of antibiogram profiling of rotten fruits, majority exhibited resistance against Erythromycin, Vancomycin and Amoxycillin and showing sensitivity against Ciprofloxacin and Ceftriaxone. It was
observed in the current study that 100% isolates were resistant against Erythromycin, followed by Amoxycillin 90.63% and Vancomycin 86.25%, where only 35.27% isolates were resistant against Ciprofloxacin. In case of sensitivity 64.73% isolates were sensitive against Ciprofloxacin followed by Ceftriaxone 66.25%. on the contrary, in case of fresh leafy and salad vegetables, the isolated organisms were tested against antibiotics among which Imipenem showed the highest sensitivity (86%), followed by Ceftriaxone (100%), Nitrofurantoin (94%), Erythromycin (89%) and Amoxicillin (83%) had the highest resistance against the isolated organisms; however most of the isolates showed a multi-drug resistance pattern and they were resistant to at least four drugs.