... or even a sow in oestrus is not always feasible under field conditions, particularly when the... more ... or even a sow in oestrus is not always feasible under field conditions, particularly when the subject is a mature, intractable boar (Fraser 1971). ... to the animals in two phases: first, xylazine (Sedomin 10 per cent; Konig) and diazepam (Diazepan 5 mg/ml; Lamar) were administered ...
A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic bo... more A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic boars (Bane 1961, Bane and Nicander 1965, 1966). Several sperm tail defects have been described in semen obtained from sterile boars, such as coiled-tail sperm (Holt 1982), and abaxial implantation of the middle piece in spermatozoa and spermatids (Thilander and others 1985). In all these conditions, over 95 per cent of the spermatozoa were affected. The tail-stump defect has been recorded in bulls of different breeds (Blom 1976, Williams 1987, Peet and Mullins 1991), and sterile human sperm (Baccetti and others 1993). This short communication describes this defect in the semen of a wild boar in captivity. The wild boar was two years old when examined; it was in good physical condition, and had
A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic bo... more A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic boars (Bane 1961, Bane and Nicander 1965, 1966). Several sperm tail defects have been described in semen obtained from sterile boars, such as coiled-tail sperm (Holt 1982), and abaxial implantation of the middle piece in spermatozoa and spermatids (Thilander and others 1985). In all these conditions, over 95 per cent of the spermatozoa were affected. The tail-stump defect has been recorded in bulls of different breeds (Blom 1976, Williams 1987, Peet and Mullins 1991), and sterile human sperm (Baccetti and others 1993). This short communication describes this defect in the semen of a wild boar in captivity. The wild boar was two years old when examined; it was in good physical condition, and had
The main goal of this work was to check the possibility of transferring useful artificial reprodu... more The main goal of this work was to check the possibility of transferring useful artificial reproductive techniques from domestic species to similar wild ones. Captivity muflons were used. Semen quality tests were performed, obtaining a direct relation between it and the dominance in the group. 0,2 ml pellets, with 150 millions sperm were frozen, with a Tris - skim milk - lactose extender1-9, and stored in liquid N2. Morphology, vitality, acrosome integrity, physiologic membrane integrity, and thermal resistance tests were performed. All doses got acceptable values for Artificial Insemination. The results obtained noticed that the applied method is useful for muflon semen cryopreservation.
The objective of this study was to evaluate the effect of the thawing procedure on deer semen fre... more The objective of this study was to evaluate the effect of the thawing procedure on deer semen freezability. Frozen semen from the Genetic Resource Bank (GRB) of the Zoological Park of Buenos Aires (Argentina) was used. Seven mature stags (two red deer, two Père David's deer and three fallow deer) were used as semen donors. Semen was diluted with a TRIS-egg yolk medium, packed in 0.25 ml straws and frozen in nitrogen vapour. For thawing, the frozen straws were subjected to the following procedures: (I) 70 degrees C, 5s; (II) 50 degrees C, 8s and (III) 37 degrees C, 10s. Freeze-thaw motility percentage (FMP) and spermatozoa rating (FMR) were determined subjectively. Viability and acrosome integrity (NAR) were also assessed and the hypo-osmotic swelling test (HOST) was used to assess membrane integrity. Freeze-thaw motility percentage, FMR and NAR were assessed after an incubation of 1h in citrate-yolk at 42 degrees C, and FMP and FMR after 2h of incubation under the same conditions. The thawing procedure did not have an effect on the seminal characteristics evaluated immediately after this process. However, differences in FMP after 2h of incubation (P<0.05) were found between the procedures, with the best overall recovery rates after freezing and thawing found with the use of protocols II (intermediate thawing) and III (slow thawing). Therefore, thawing protocols II and III, those that provide intermediate and slow thawing rates, were the most beneficial for semen thawing of the different cervid species analysed in this study.
The aim of this research was to evaluate two different diluents for sperm cryopreservation and to... more The aim of this research was to evaluate two different diluents for sperm cryopreservation and to study functional parameters in relation to the response to heparin, lysophosphatidylcholine and progesterone, in frozen-thawed semen of fallow deer (Dama dama) during the reproductive season (brama). In this way, fallow deer can be used as a biological model of endangered cervids. Semen was obtained by electroejaculation. Heparin, progesterone and lysophosphatidylcholine were used as capacitation and acrosome reaction inducers, respectively. Capacitation and acrosome reaction were evaluated by chlorotetracycline epifluorescence technique (CTC), membrane integrity by Hypo-osmotic swelling test (HOS) and viability and acrosome integrity by trypan blue stain/DIC. Data was analyzed by ANOVA and Tukey Test (P < 0.05). Semen was cryopreserved in different diluents and Fructose-Tris-Glycine extender was selected. Capacitation with heparin at different incubation times determined that the highest capacitation percentage was obtained at 45 minutes incubation. Progesterone (1 'M) and lysophosphatidylcholine in heparin capacitated sperm induced acrosome reaction (P < 0.05). This study contributes to improve cryopreservation methods and to increase the knowledge about capacitation and acrosome reaction in vitro in deer spermatozoa, allowing an advance in the development of reproductive biotechnologies.
... En cada portaobjeto se midieron números similares de núcleos espermáticos de ciervo colorado ... more ... En cada portaobjeto se midieron números similares de núcleos espermáticos de ciervo colorado y eritrocitos de pollo totalizando 60 núcleos (30 de ... 14. Giuliano, SM; Ferrari, MR; Spirito, SE;Roldán, J.; Fernández, HA Contenido de ADN de llama (Lama glama) y estudio de la ...
... or even a sow in oestrus is not always feasible under field conditions, particularly when the... more ... or even a sow in oestrus is not always feasible under field conditions, particularly when the subject is a mature, intractable boar (Fraser 1971). ... to the animals in two phases: first, xylazine (Sedomin 10 per cent; Konig) and diazepam (Diazepan 5 mg/ml; Lamar) were administered ...
A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic bo... more A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic boars (Bane 1961, Bane and Nicander 1965, 1966). Several sperm tail defects have been described in semen obtained from sterile boars, such as coiled-tail sperm (Holt 1982), and abaxial implantation of the middle piece in spermatozoa and spermatids (Thilander and others 1985). In all these conditions, over 95 per cent of the spermatozoa were affected. The tail-stump defect has been recorded in bulls of different breeds (Blom 1976, Williams 1987, Peet and Mullins 1991), and sterile human sperm (Baccetti and others 1993). This short communication describes this defect in the semen of a wild boar in captivity. The wild boar was two years old when examined; it was in good physical condition, and had
A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic bo... more A NUMBER of specific morphological abnormalities of spermatozoa have been reported in domestic boars (Bane 1961, Bane and Nicander 1965, 1966). Several sperm tail defects have been described in semen obtained from sterile boars, such as coiled-tail sperm (Holt 1982), and abaxial implantation of the middle piece in spermatozoa and spermatids (Thilander and others 1985). In all these conditions, over 95 per cent of the spermatozoa were affected. The tail-stump defect has been recorded in bulls of different breeds (Blom 1976, Williams 1987, Peet and Mullins 1991), and sterile human sperm (Baccetti and others 1993). This short communication describes this defect in the semen of a wild boar in captivity. The wild boar was two years old when examined; it was in good physical condition, and had
The main goal of this work was to check the possibility of transferring useful artificial reprodu... more The main goal of this work was to check the possibility of transferring useful artificial reproductive techniques from domestic species to similar wild ones. Captivity muflons were used. Semen quality tests were performed, obtaining a direct relation between it and the dominance in the group. 0,2 ml pellets, with 150 millions sperm were frozen, with a Tris - skim milk - lactose extender1-9, and stored in liquid N2. Morphology, vitality, acrosome integrity, physiologic membrane integrity, and thermal resistance tests were performed. All doses got acceptable values for Artificial Insemination. The results obtained noticed that the applied method is useful for muflon semen cryopreservation.
The objective of this study was to evaluate the effect of the thawing procedure on deer semen fre... more The objective of this study was to evaluate the effect of the thawing procedure on deer semen freezability. Frozen semen from the Genetic Resource Bank (GRB) of the Zoological Park of Buenos Aires (Argentina) was used. Seven mature stags (two red deer, two Père David's deer and three fallow deer) were used as semen donors. Semen was diluted with a TRIS-egg yolk medium, packed in 0.25 ml straws and frozen in nitrogen vapour. For thawing, the frozen straws were subjected to the following procedures: (I) 70 degrees C, 5s; (II) 50 degrees C, 8s and (III) 37 degrees C, 10s. Freeze-thaw motility percentage (FMP) and spermatozoa rating (FMR) were determined subjectively. Viability and acrosome integrity (NAR) were also assessed and the hypo-osmotic swelling test (HOST) was used to assess membrane integrity. Freeze-thaw motility percentage, FMR and NAR were assessed after an incubation of 1h in citrate-yolk at 42 degrees C, and FMP and FMR after 2h of incubation under the same conditions. The thawing procedure did not have an effect on the seminal characteristics evaluated immediately after this process. However, differences in FMP after 2h of incubation (P<0.05) were found between the procedures, with the best overall recovery rates after freezing and thawing found with the use of protocols II (intermediate thawing) and III (slow thawing). Therefore, thawing protocols II and III, those that provide intermediate and slow thawing rates, were the most beneficial for semen thawing of the different cervid species analysed in this study.
The aim of this research was to evaluate two different diluents for sperm cryopreservation and to... more The aim of this research was to evaluate two different diluents for sperm cryopreservation and to study functional parameters in relation to the response to heparin, lysophosphatidylcholine and progesterone, in frozen-thawed semen of fallow deer (Dama dama) during the reproductive season (brama). In this way, fallow deer can be used as a biological model of endangered cervids. Semen was obtained by electroejaculation. Heparin, progesterone and lysophosphatidylcholine were used as capacitation and acrosome reaction inducers, respectively. Capacitation and acrosome reaction were evaluated by chlorotetracycline epifluorescence technique (CTC), membrane integrity by Hypo-osmotic swelling test (HOS) and viability and acrosome integrity by trypan blue stain/DIC. Data was analyzed by ANOVA and Tukey Test (P < 0.05). Semen was cryopreserved in different diluents and Fructose-Tris-Glycine extender was selected. Capacitation with heparin at different incubation times determined that the highest capacitation percentage was obtained at 45 minutes incubation. Progesterone (1 'M) and lysophosphatidylcholine in heparin capacitated sperm induced acrosome reaction (P < 0.05). This study contributes to improve cryopreservation methods and to increase the knowledge about capacitation and acrosome reaction in vitro in deer spermatozoa, allowing an advance in the development of reproductive biotechnologies.
... En cada portaobjeto se midieron números similares de núcleos espermáticos de ciervo colorado ... more ... En cada portaobjeto se midieron números similares de núcleos espermáticos de ciervo colorado y eritrocitos de pollo totalizando 60 núcleos (30 de ... 14. Giuliano, SM; Ferrari, MR; Spirito, SE;Roldán, J.; Fernández, HA Contenido de ADN de llama (Lama glama) y estudio de la ...
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