Present results documented the genetic variability of 6 different species of predatory coccinelli... more Present results documented the genetic variability of 6 different species of predatory coccinellid beetles viz., Coccinella septempunctata (CS), Cheilomenes sexamaculata (MS), Coccinella transversalis (CT), Micraspis discolour (MD), Harmonia dimidiata (Black) (HD), Propylea species (PQ) along with a variant of Harmonia dimidiata (Spotted) (HY) in Tarai region of Pantnagar. Results further revealed that out of the eight different primers of cytochrome oxidase gene, primer P1 was found to be a suitable marker to distinguish variability between 6 species of coccinellids with highest RP (2.71) and MI (2.19) values. The variant of H. dimidiata (spotted) also had genetic difference in their COI region and this provided firsthand information about the diversity of predatory coccinellids in Pantnagar, Tarai region of Uttarakhand.
Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively contr... more Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively controls different lepidopteran pests of various crop ecosystems. Hence, proper utilization of this predator in biological control of insect pests is the need of the hour. In this regard, experiment was conducted to study the suitability of freeze killed larvae of Spodoptera litura and Corcyra cephalonica for mass rearing of E. furcellata based on different biological attributes. The total adult period of predator on live larvae varied significantly with frozen larvae of both the prey. The maximum consumption rate of adult predatory bug was recorded on freeze killed larvae of S. litura while minimum consumption rate was observed on live larvae of C. cephalonica. The results revealed that E. furcellata can be successfully reared on the live and frozen larvae of both the prey in laboratory conditions. Therefore, live and freeze killed larvae of S. litura and C. cephalonica may be exploited for ...
Present study deliberates on the geographical distribution and genetic variability of the predato... more Present study deliberates on the geographical distribution and genetic variability of the predatory stink bug Andrallus spinidens (F.) from different crop ecosystems of north west Himalaya. Andrallus spinidens is an important biological control agent as it predates on lepidopteran larvae. Samples of this bug were collected from five locations of plains and hilly areas of Uttarakhand and subjected to RAPD-PCR analysis. The results revealed that of the 12 RAPD primers evaluated 10 primers generated 117 loci with 0.26 polymorphism information content (PIC), 2.75 marker index (MI), 4.67 resolving power (RP). RAPD primer, EF4 was found to be efficient in deciphering the variability with maximum RP values of 7.71 with 4.37 MI and 0.39 PIC values. The samples were found clustered locationwise with 0.26–0.95 similarity coefficient during cluster analysis using UPGMA method. The pairwise comparison analysis (PCA) also supported the clustering pattern obtained. The results reveal that genetically distant populations of A. spinidens prevail in all the five locations studied.
Fall armyworm, Spodoptera frugiperda (J.E. Smith) is a noxious lepidopterous pest of maize crop. ... more Fall armyworm, Spodoptera frugiperda (J.E. Smith) is a noxious lepidopterous pest of maize crop. Northern region of India was devoid from incidences of S. frugiperda until now in 2019 it has been observed in Uttarakhand state for the first time at N.E.B. Corp Research Center, GBPUA&T Pantnagar, Udham Singh Nagar during the routine survey in the maize field. The morphological and molecular tools were used for the identification of the Pantnagar pest sample. The mtCOI gene was used as molecular marker and it was found that the Pantnagar sample sequence showed 100% similarity after BLAST with the sequence accession No. GU095403.1 (658 bp) and MH704433 (658bp) of S. frugiperda from Canada and Karnataka (India), respectively. The mtCOI consensus sequences generated for Pantnagar sample of 630 bp has been deposited in National Centre for Biotechnology Information GenBank databases as S. frugiperda sequence of Pantnagar sample. Morphological and molecular characterization of Pantnagar pest...
Investigation was carried out during Kharif, 2017 at GB Pant University of Agriculture & Technolo... more Investigation was carried out during Kharif, 2017 at GB Pant University of Agriculture & Technology, Pantnagar, India to study seasonal incidence of insect pests and their natural enemies on soybean crop. The density of defoliators i.e. Spodoptera litura and Spilosoma obliqua increased gradually to a peak of 12.5 and 3.9 larva/meter row length during the last week of August and Thysanoplusia orichalcea with 6.1 larva/mrl during first week of September and exhibit positive correlation with maximum temperature and RH%. The peak density of sucking pest (Bemisia tabaci and Aphis gossypii) was observed during mid of August and exhibit positive correlation with maximum temperature, RH% and evaporation. Stem fly infestation persisted till maturity of the crop. Natural enemies i.e. Coccinella septumpunctata and Eocanthecona furcellata occurred during third week of August. Results revealed that temperature, relative humidity and evaporation act as limiting factor for population buildup of in...
Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively contr... more Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively controls different lepidopteran pests of various crop ecosystems. Hence, proper utilization of this predator in biological control of insect pests is the need of the hour. In this regard, experiment was conducted to study the suitability of freeze killed larvae of Spodoptera litura and Corcyra cephalonica for mass rearing of E. furcellata based on different biological attributes. The total adult period of predator on live larvae varied significantly with frozen larvae of both the prey. The maximum consumption rate of adult predatory bug was recorded on freeze killed larvae of S. litura while minimum consumption rate was observed on live larvae of C. cephalonica. The results revealed that E. furcellata can be successfully reared on the live and frozen larvae of both the prey in laboratory conditions. Therefore, live and freeze killed larvae of S. litura and C. cephalonica may be exploited for mass rearing of predatory bug in laboratory conditions.
Present results documented the genetic variability of 6 different species of predatory coccinelli... more Present results documented the genetic variability of 6 different species of predatory coccinellid beetles viz., Coccinella septempunctata (CS), Cheilomenes sexamaculata (MS), Coccinella transversalis (CT), Micraspis discolour (MD), Harmonia dimidiata (Black) (HD), Propylea species (PQ) along with a variant of Harmonia dimidiata (Spotted) (HY) in Tarai region of Pantnagar. Results further revealed that out of the eight different primers of cytochrome oxidase gene, primer P1 was found to be a suitable marker to distinguish variability between 6 species of coccinellids with highest RP (2.71) and MI (2.19) values. The variant of H. dimidiata (spotted) also had genetic difference in their COI region and this provided firsthand information about the diversity of predatory coccinellids in Pantnagar, Tarai region of Uttarakhand.
Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively contr... more Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively controls different lepidopteran pests of various crop ecosystems. Hence, proper utilization of this predator in biological control of insect pests is the need of the hour. In this regard, experiment was conducted to study the suitability of freeze killed larvae of Spodoptera litura and Corcyra cephalonica for mass rearing of E. furcellata based on different biological attributes. The total adult period of predator on live larvae varied significantly with frozen larvae of both the prey. The maximum consumption rate of adult predatory bug was recorded on freeze killed larvae of S. litura while minimum consumption rate was observed on live larvae of C. cephalonica. The results revealed that E. furcellata can be successfully reared on the live and frozen larvae of both the prey in laboratory conditions. Therefore, live and freeze killed larvae of S. litura and C. cephalonica may be exploited for ...
Present study deliberates on the geographical distribution and genetic variability of the predato... more Present study deliberates on the geographical distribution and genetic variability of the predatory stink bug Andrallus spinidens (F.) from different crop ecosystems of north west Himalaya. Andrallus spinidens is an important biological control agent as it predates on lepidopteran larvae. Samples of this bug were collected from five locations of plains and hilly areas of Uttarakhand and subjected to RAPD-PCR analysis. The results revealed that of the 12 RAPD primers evaluated 10 primers generated 117 loci with 0.26 polymorphism information content (PIC), 2.75 marker index (MI), 4.67 resolving power (RP). RAPD primer, EF4 was found to be efficient in deciphering the variability with maximum RP values of 7.71 with 4.37 MI and 0.39 PIC values. The samples were found clustered locationwise with 0.26–0.95 similarity coefficient during cluster analysis using UPGMA method. The pairwise comparison analysis (PCA) also supported the clustering pattern obtained. The results reveal that genetically distant populations of A. spinidens prevail in all the five locations studied.
Fall armyworm, Spodoptera frugiperda (J.E. Smith) is a noxious lepidopterous pest of maize crop. ... more Fall armyworm, Spodoptera frugiperda (J.E. Smith) is a noxious lepidopterous pest of maize crop. Northern region of India was devoid from incidences of S. frugiperda until now in 2019 it has been observed in Uttarakhand state for the first time at N.E.B. Corp Research Center, GBPUA&T Pantnagar, Udham Singh Nagar during the routine survey in the maize field. The morphological and molecular tools were used for the identification of the Pantnagar pest sample. The mtCOI gene was used as molecular marker and it was found that the Pantnagar sample sequence showed 100% similarity after BLAST with the sequence accession No. GU095403.1 (658 bp) and MH704433 (658bp) of S. frugiperda from Canada and Karnataka (India), respectively. The mtCOI consensus sequences generated for Pantnagar sample of 630 bp has been deposited in National Centre for Biotechnology Information GenBank databases as S. frugiperda sequence of Pantnagar sample. Morphological and molecular characterization of Pantnagar pest...
Investigation was carried out during Kharif, 2017 at GB Pant University of Agriculture & Technolo... more Investigation was carried out during Kharif, 2017 at GB Pant University of Agriculture & Technology, Pantnagar, India to study seasonal incidence of insect pests and their natural enemies on soybean crop. The density of defoliators i.e. Spodoptera litura and Spilosoma obliqua increased gradually to a peak of 12.5 and 3.9 larva/meter row length during the last week of August and Thysanoplusia orichalcea with 6.1 larva/mrl during first week of September and exhibit positive correlation with maximum temperature and RH%. The peak density of sucking pest (Bemisia tabaci and Aphis gossypii) was observed during mid of August and exhibit positive correlation with maximum temperature, RH% and evaporation. Stem fly infestation persisted till maturity of the crop. Natural enemies i.e. Coccinella septumpunctata and Eocanthecona furcellata occurred during third week of August. Results revealed that temperature, relative humidity and evaporation act as limiting factor for population buildup of in...
Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively contr... more Pentatomid predatory bug, Eocanthecona furcellata is a potential predator which effectively controls different lepidopteran pests of various crop ecosystems. Hence, proper utilization of this predator in biological control of insect pests is the need of the hour. In this regard, experiment was conducted to study the suitability of freeze killed larvae of Spodoptera litura and Corcyra cephalonica for mass rearing of E. furcellata based on different biological attributes. The total adult period of predator on live larvae varied significantly with frozen larvae of both the prey. The maximum consumption rate of adult predatory bug was recorded on freeze killed larvae of S. litura while minimum consumption rate was observed on live larvae of C. cephalonica. The results revealed that E. furcellata can be successfully reared on the live and frozen larvae of both the prey in laboratory conditions. Therefore, live and freeze killed larvae of S. litura and C. cephalonica may be exploited for mass rearing of predatory bug in laboratory conditions.
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