Fascioliasis is an emerging and important food and water-borne disease in human communities which... more Fascioliasis is an emerging and important food and water-borne disease in human communities which has become one of the most important health challenges in countries, like Iran. It causes weight loss, a decrease in feed conversion ratio as well as milk and meat production, and also reduces fertility in animals the prevalence of fasciolosis is increasing in some regions of the world due to various factors. Different methods have been used for the detection of Fasciola hepatica in animals. This study is the first to detect F. hepatica in Lori sheep using polymerase chain reaction (PCR) and conventional diagnostic methods in Western Iran. During three months, 195 fecal samples were collected from sheep in Lorestan province, Iran, using the stratified random sampling method. The conventional diagnostic methods, including wet mount microscopic examination and concentration assays, as well as the PCR technique targeting the intergenic spacer gene of F. hepatica, were used for the detectio...
Introduction: Linguatula serrata is a zoonotic parasite, belonging to the class Pentastomida. The... more Introduction: Linguatula serrata is a zoonotic parasite, belonging to the class Pentastomida. The major aim of this study was to evaluate the impact of sodium chloride (NaCl) and heat on survival time of Linguatula serrata nymphs. Materials & Methods: Thirty nymphs (10 in triplicate) were separately transferred to plastic tubes, containing different concentrations of NaCl solution (2%, 5% and 10%). Meanwhile, 30 nymphs in tubes containing Phosphate Buffer Saline (PBS) were separately treated by +50°C, +60°C and +72°C. As control group, thirty nymphs were stored in PBS at +4°C. The effects of different conditions on survival time of the nymphs were evaluated by observing their motility in different periods of time. Results: The survival time of the nymphs stored in 10% NaCl solution was too short and all of them were dead after 3 hours. But the other ones maintained in 2% NaCl solution were significantly more resistant (p<0.05) and were survived for 2 days. All the nymphs pertaini...
Fascioliasis is a parasitic infection caused by Fasciola spp. in humans and animals. Despite sign... more Fascioliasis is a parasitic infection caused by Fasciola spp. in humans and animals. Despite significant advances in vaccination and new therapeutic agents, little attention has been paid to validate methods for the diagnosis of fascioliasis in animals. This study aimed to compare the loop‐mediated isothermal amplification (LAMP) technique with PCR assay for the diagnosis of F. hepatica in sheep. In this cross‐sectional study, 195 stool samples were collected from sheep for 3 months in Lorestan province, West of Iran. Specimens’ parasitological examination was performed by using the direct wet mount and formalin‐ether concentration method. After DNA extraction from the samples, molecular analysis was done using PCR and LAMP techniques based on the Fasciola ribosomal intergenic spacer (IGS) sequence. Of 195 specimens of sheep, 11 specimens were identified as F. hepatica‐positive infection by using microscopic, PCR and LAMP assays. Kappa agreement test results showed that there was a ...
Objective: Linguatulosis is one of the food-borne parasitic zoonoses. Human infection may occurs ... more Objective: Linguatulosis is one of the food-borne parasitic zoonoses. Human infection may occurs following consumption of the raw or undercooked infected offal of slaughtered ruminants, especially mesenteric lymph nodes (MLNs), liver and lung. The main objective of this study was to evaluate the effect of different temperature conditions on survival time of Linguatula serrata nymphs. Material and Methods: Fifteen infected MLNs (5 in triplicate) were separately put in plastic bags and were treated by +50°C, +60°C, +72°C and -18°C through water bath or freezer. Meanwhile, fifteen infected MLNs (5 in triplicate) were stored in refrigerator (+4°C) as control group. The survival time of the nymphs was evaluated by observing their motility and wriggle under a stereomicroscope in different periods of time. Results: The infected MLNs were mainly edematous, hemorrhagic and dark comparing to the non-infected ones. The survival time of the nymphs stored at the high temperatures (+50°C, +60°C a...
There are several methods, such as vaccination, to control visceral leishmaniasis. Although there... more There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular and humoral immunity apparently is the best way. The aim of this study was cloning and expression of LACK gene, a 36kD protein, as a candidate protein for vaccination against Iranian L. infantum. Iranian strain of L. infantum [MCAN/IR/07/Moheb-gh] was used as a template for PCR to amplify LACK gene. The LACK gene was cloned in pTZ57R/T vector and after confirmation it was digested by restriction enzymes (BamH1) and cloned in pcDNA3.1 expression vector. Recombinant plasmid was extracted and analyzed by sequencing, restriction digestion analysis and PCR reaction. The pc- LACK recombinant plasmid was purified from transformed E.coli (DH5α) and its expression was analyzed by SDS-PAGE and Western blot. The results of sequencing, restriction digestion analysis and PCR reaction revealed that LACK gene was cloned cor...
Fascioliasis is an emerging and important food and water-borne disease in human communities which... more Fascioliasis is an emerging and important food and water-borne disease in human communities which has become one of the most important health challenges in countries, like Iran. It causes weight loss, a decrease in feed conversion ratio as well as milk and meat production, and also reduces fertility in animals the prevalence of fasciolosis is increasing in some regions of the world due to various factors. Different methods have been used for the detection of Fasciola hepatica in animals. This study is the first to detect F. hepatica in Lori sheep using polymerase chain reaction (PCR) and conventional diagnostic methods in Western Iran. During three months, 195 fecal samples were collected from sheep in Lorestan province, Iran, using the stratified random sampling method. The conventional diagnostic methods, including wet mount microscopic examination and concentration assays, as well as the PCR technique targeting the intergenic spacer gene of F. hepatica, were used for the detectio...
Introduction: Linguatula serrata is a zoonotic parasite, belonging to the class Pentastomida. The... more Introduction: Linguatula serrata is a zoonotic parasite, belonging to the class Pentastomida. The major aim of this study was to evaluate the impact of sodium chloride (NaCl) and heat on survival time of Linguatula serrata nymphs. Materials & Methods: Thirty nymphs (10 in triplicate) were separately transferred to plastic tubes, containing different concentrations of NaCl solution (2%, 5% and 10%). Meanwhile, 30 nymphs in tubes containing Phosphate Buffer Saline (PBS) were separately treated by +50°C, +60°C and +72°C. As control group, thirty nymphs were stored in PBS at +4°C. The effects of different conditions on survival time of the nymphs were evaluated by observing their motility in different periods of time. Results: The survival time of the nymphs stored in 10% NaCl solution was too short and all of them were dead after 3 hours. But the other ones maintained in 2% NaCl solution were significantly more resistant (p<0.05) and were survived for 2 days. All the nymphs pertaini...
Fascioliasis is a parasitic infection caused by Fasciola spp. in humans and animals. Despite sign... more Fascioliasis is a parasitic infection caused by Fasciola spp. in humans and animals. Despite significant advances in vaccination and new therapeutic agents, little attention has been paid to validate methods for the diagnosis of fascioliasis in animals. This study aimed to compare the loop‐mediated isothermal amplification (LAMP) technique with PCR assay for the diagnosis of F. hepatica in sheep. In this cross‐sectional study, 195 stool samples were collected from sheep for 3 months in Lorestan province, West of Iran. Specimens’ parasitological examination was performed by using the direct wet mount and formalin‐ether concentration method. After DNA extraction from the samples, molecular analysis was done using PCR and LAMP techniques based on the Fasciola ribosomal intergenic spacer (IGS) sequence. Of 195 specimens of sheep, 11 specimens were identified as F. hepatica‐positive infection by using microscopic, PCR and LAMP assays. Kappa agreement test results showed that there was a ...
Objective: Linguatulosis is one of the food-borne parasitic zoonoses. Human infection may occurs ... more Objective: Linguatulosis is one of the food-borne parasitic zoonoses. Human infection may occurs following consumption of the raw or undercooked infected offal of slaughtered ruminants, especially mesenteric lymph nodes (MLNs), liver and lung. The main objective of this study was to evaluate the effect of different temperature conditions on survival time of Linguatula serrata nymphs. Material and Methods: Fifteen infected MLNs (5 in triplicate) were separately put in plastic bags and were treated by +50°C, +60°C, +72°C and -18°C through water bath or freezer. Meanwhile, fifteen infected MLNs (5 in triplicate) were stored in refrigerator (+4°C) as control group. The survival time of the nymphs was evaluated by observing their motility and wriggle under a stereomicroscope in different periods of time. Results: The infected MLNs were mainly edematous, hemorrhagic and dark comparing to the non-infected ones. The survival time of the nymphs stored at the high temperatures (+50°C, +60°C a...
There are several methods, such as vaccination, to control visceral leishmaniasis. Although there... more There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular and humoral immunity apparently is the best way. The aim of this study was cloning and expression of LACK gene, a 36kD protein, as a candidate protein for vaccination against Iranian L. infantum. Iranian strain of L. infantum [MCAN/IR/07/Moheb-gh] was used as a template for PCR to amplify LACK gene. The LACK gene was cloned in pTZ57R/T vector and after confirmation it was digested by restriction enzymes (BamH1) and cloned in pcDNA3.1 expression vector. Recombinant plasmid was extracted and analyzed by sequencing, restriction digestion analysis and PCR reaction. The pc- LACK recombinant plasmid was purified from transformed E.coli (DH5α) and its expression was analyzed by SDS-PAGE and Western blot. The results of sequencing, restriction digestion analysis and PCR reaction revealed that LACK gene was cloned cor...
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