Introduction: Antimicrobial photodynamic therapy (aPDT) is an adjunctive non-invasive procedure f... more Introduction: Antimicrobial photodynamic therapy (aPDT) is an adjunctive non-invasive procedure for the management of periodontal tissue infection and deep periodontal pocket decontamination. However, the effects of this procedure on periodontal cells like osteoblasts that play a role in periodontal tissue repair and regeneration is not yet clear. Aim: This study aimed to investigate aPDT based on indocyanine green (ICG) on MG-63 osteoblast-like cells in vitro. Materials and methods: MG-63 cells were treated in 9 different groups: 1) Control (untreated cells), 2) ICG alone at a concentration of 1000 µg/mL, 3) ICG alone at a concentration of 2000 µg/mL, 4) diode laser irradiation alone for 30 s, 5) laser irradiation for 30 s combined with ICG with a concentration of 1000 µg/mL, 6) laser irradiation for 30 s combined with ICG at a concentration of 2000 µg/mL, 7) laser irradiation alone for 60 s, 8) laser irradiation for 60 s combined with ICG at a concentration of 1000 µg/mL, and 9) l...
Background and Aim: Allografts are a group of grafting materials in two types of DFDBAs and FDBAs... more Background and Aim: Allografts are a group of grafting materials in two types of DFDBAs and FDBAs with various applications in restoration of osseous defects. Considering high price of foreign brands of these materials, we decided to launch a study and compare 2 types of FDBA and DFDBA osseous powders manufactured by Faravardehaye Peyvandi Iran Company and their application in restoration of calvaria bone damages in rabbits. Materials and Methods: The research performed on randomized clinical trial on 12 white rabbits.We made 4 full thickness 8 millimeter defects in calvaria bones of each rabbit. Then, in two groups, DFDBA and FDBA were used and other two groups were the positive and negative control group. Within the 2 consecutive months (2,4,6, and 8 weeks) every session, 5 rabbits were sacrificed and removed the calvaria bone for histologic and histomorphometric study. Data were analyzed using Friedman and Kruskal-Wallis and Wilcoxon tests. We utilized SPSS ver:20 software. Resul...
Sana Baghizadeh, Dental student, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad Univ... more Sana Baghizadeh, Dental student, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Email: sanabaghizadeh@ yahoo.com Background and Aim: The most common etiology of gingivitis is accumulation of bacterial plaque. Complete removal of microbial plaque by mechanical procedures is not possible in some cases; for example, aged and disabled patients might not be capable of removing the bacterial plaques properly. Also, chemical mouthwashes have some adverse effects. Therefore, finding a new treatment approach would be helpful. The global focus on the use of herbal medicine for treatment of different health conditions is on the rise. The present study aimed at searching and collecting scientific evidence regarding medicinal herbs to treat gingivitis and periodontitis. Materials and Methods: In this review, an electronic search of the literature was conducted through PubMed, Scopus, Google Scholar, and Wiley online library databases. Studies were considered...
Background Inflammatory bone resorption in periodontitis can lead to tooth loss. Systemic adminis... more Background Inflammatory bone resorption in periodontitis can lead to tooth loss. Systemic administration of bisphosphonates such as risedronate for preventing bone resorption can cause adverse effects. ALG and PLGA microparticles have been studied as drug delivery systems for sustained release of drugs. Therefore, the release pattern of risedronate from PLGA microparticles embedded with ALG was studied as a drug delivery system for sustained release of the drug, which can be used in local administrations. Methods Risedronate-containing PLGA microparticles were fabricated using double emulsion solvent evaporation technique. Ionic cross-linking method was used to fabricate risedronate-loaded ALG. Risedronate-containing PLGA microparticles were then coated with ALG. The calibration curve of risedronate was traced to measure EE and study the release pattern. SEM imaging was carried out, and cell toxicity was examined using MTT assay. Statistical analysis of data was carried out using SP...
Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering ... more Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations ofZingiber officinaleextract on proliferation ofStreptococcus mutansandStreptococcus sanguinisin vitro.Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛof each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛof each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as...
Journal of Dental Research, Dental Clinics, Dental Prospects, 2017
Background. Lateral ridge augmentation is conventionally accomplished by means of autogenous bone... more Background. Lateral ridge augmentation is conventionally accomplished by means of autogenous bone grafts. However, due to its complications, the application of autogenous bone graft substitutes, e.g. mineralized corticocancellous allograft, is ecommended. Methods. In the present study, twelve patients were included, with insufficient alveolar ridge widths in the designated sites for dental implant placement. During the primary surgery, mineralized corticocancellous block allografts were fixed in deficient sites with titanium screws and resorbable collagen membranes were used to cover the blocks. After a period of six months, a flap was raised and variations in ridge width values was measured. Finally, a micro-biopsy was obtained from the sites for histologic investigation prior to preparing them for subsequent implant placement. Results. All the applied blocks were incorporated into the underlying bone except for one. A statistically significant difference was seen between the avera...
Introduction: This study sought to assess the cytotoxic effects of nanoparticulate and micropart... more Introduction: This study sought to assess the cytotoxic effects of nanoparticulate and microparticulate calcium sodium phosphosilicate mouthwashes on human gingival fibroblasts (HGFs). Methods: This in vitro study was conducted on HGFs isolated and cultured in a 48-well plate containing standard culture medium for evaluation of four concentrations of the two mouthwashes at two time points plus a positive and a negative control group. The HGFs were exposed to 0.001, 0.01, 0.1 and 1 mg/mL concentrations of mouthwashes for 1 and 24 hours. Positive and negative control cells were exposed to saline and distilled water, respectively. Cell viability was assessed using the methyl thiazolyl tetrazolium (MTT) assay, and the number of viable cells was counted in triplicate using a cell counter after transfection (trypsin-EDTA 0.25%, 20 minutes) and exposure to trypan blue. The optical density (OD) values were read by ELISA reader and analyzed using the Kruskal-Wallis test. Results: Number of ...
Introduction: Mechanical debridement of diseased root surfaces produces a smear layer that encomp... more Introduction: Mechanical debridement of diseased root surfaces produces a smear layer that encompass microorganisms and residual cementum which may interfere with periodontal healing and regeneration of connective tissue attachment. Accordingly, this study aimed to determine impact of 940nm diode laser on adhesion of fibroblasts to root surface of extracted teeth from patients with chronic periodontitis. Methods: Twenty extracted single-rooted teeth with hopeless prognosis were collected and debrided with hand curettes. Afterward, two specimens were obtained from each tooth by splitting them with a sterile diamond disk. Samples were submerged in fibroblast suspension and randomly divided into two groups. Group A comprised of 20 specimens subjected to scaling and root planing only and group B included 20 specimens which received SRP and and 940 nm diode laser irradiation. The adhesion of fibroblasts was investigated by MTT and cell morphology was assessed with scanning electron micro...
Objective(s): The present study aimed to assess the in-vitro efficacy of nanoparticulate calcium ... more Objective(s): The present study aimed to assess the in-vitro efficacy of nanoparticulate calcium sodium phosphosilicate mouthwash in the obstruction of dentinal tubules.Materials and Methods: This in-vitro, study was conducted on 120 sections obtained from extracted human premolars, which were etched with citric acid for two minutes and rinsed with distilled water. Afterwards, the sections were randomly divided into two groups (60 per each) of nanoparticulate and regular mouthwash. In addition, each group was divided into six subgroups of 10. In the nanoparticulate mouthwash subgroups, one subgroup (n=10) was evaluated using scanning electron microscopy (SEM). The remaining five subgroups were immersed in artificial saliva for different time periods and inspected using SEM. The control subgroups were exposed to regular mouthwash. The diameters and number of the open dentinal tubules were evaluated and compared using two-way analysis of variance (ANOVA).Results: The mean number of th...
Statement of the Problem: Alveolar bone resorption associated with periodontal disease is a commo... more Statement of the Problem: Alveolar bone resorption associated with periodontal disease is a common finding and generally irreversible. It impairs mastication and causes esthetic problems for patients. Bisphosphonates are the most commonly used antiresorptive agents for bone diseases. Purpose: Considering the risk of bisphosphonate-related osteonecrosis of the jaw, this study aimed to assess the effect of 2% risedronate gel on calvarial bone defects in rabbits. Materials and Method: In this animal study, critical-size defects of 8mm were created in the calvaria of 20 New Zealand white rabbits. In group 1 (n=10), 2% risedronate gel was applied into the right side defect while the left side defect remained empty and served as control. In group 2 (n=10), placebo gel was applied into the right side defect, while the left side defect remained empty and served as control. Five rabbits in each group were sacrificed at 1month and the remaining five at 2 month, post-operatively, and tissue sa...
Introduction:Lateral ridge augmentations are traditionally performed using autogenous bone grafts... more Introduction:Lateral ridge augmentations are traditionally performed using autogenous bone grafts. However, the bone harvesting procedure, whether intra orally or extra orally, is accompanied by patient morbidity. So, applying bone substitute has been suggested. The aim of the present study was to compare the stability of implants in ridges augmented with mineralized cortical cancellous allograft blocks with implants in native bone using Resonance Frequency Analysis. Materials & Methods:In this clinical trial study, approved by ethical committee of Mashhad university of Medical Sciences, nine subjects with insufficient ridge width were selected. Their ridges were augmented with mineralized cortical cancellous allograft blocks. After 6 months, implants were inserted. In control group, 9 subjects with sufficient ridge width were selected. Implants were inserted in the same place as the test group. ISQ was used for measurement of implants stability on third and sixth month after implan...
Introduction: Recently, the management of dentin hypersensitivity by lasers has gained special at... more Introduction: Recently, the management of dentin hypersensitivity by lasers has gained special attention. This study aimed to assess and compare the efficacy of the 980 nm diode, Nd:YAG and Er:YAG lasers accompanied by fluoride in dentinal tubule obstruction. Methods: Twenty sound single-rooted human teeth were used for this invitro study. Forty dentinal discs were prepared of the roots and etched with 6% citric acid. One layer of fluoride varnish was applied over their surface. The sections were randomly allocated into 4 groups. The control group received no laser irradiation. Group 2 underwent 980 nm diode laser irradiation with 0.5 W power. Group 3 underwent Nd:YAG laser irradiation with 0.5 W power and group 4 underwent Er:YAG laser irradiation with 0.5 W power. All samples were then inspected under a scanning electron microscope, and the number of obstructed dentinal tubules and the diameter of open dentinal tubules in the field were determined. One-way ANOVA and Tukey’s test w...
Introduction: Antimicrobial photodynamic therapy (aPDT) is an adjunctive non-invasive procedure f... more Introduction: Antimicrobial photodynamic therapy (aPDT) is an adjunctive non-invasive procedure for the management of periodontal tissue infection and deep periodontal pocket decontamination. However, the effects of this procedure on periodontal cells like osteoblasts that play a role in periodontal tissue repair and regeneration is not yet clear. Aim: This study aimed to investigate aPDT based on indocyanine green (ICG) on MG-63 osteoblast-like cells in vitro. Materials and methods: MG-63 cells were treated in 9 different groups: 1) Control (untreated cells), 2) ICG alone at a concentration of 1000 µg/mL, 3) ICG alone at a concentration of 2000 µg/mL, 4) diode laser irradiation alone for 30 s, 5) laser irradiation for 30 s combined with ICG with a concentration of 1000 µg/mL, 6) laser irradiation for 30 s combined with ICG at a concentration of 2000 µg/mL, 7) laser irradiation alone for 60 s, 8) laser irradiation for 60 s combined with ICG at a concentration of 1000 µg/mL, and 9) l...
Background and Aim: Allografts are a group of grafting materials in two types of DFDBAs and FDBAs... more Background and Aim: Allografts are a group of grafting materials in two types of DFDBAs and FDBAs with various applications in restoration of osseous defects. Considering high price of foreign brands of these materials, we decided to launch a study and compare 2 types of FDBA and DFDBA osseous powders manufactured by Faravardehaye Peyvandi Iran Company and their application in restoration of calvaria bone damages in rabbits. Materials and Methods: The research performed on randomized clinical trial on 12 white rabbits.We made 4 full thickness 8 millimeter defects in calvaria bones of each rabbit. Then, in two groups, DFDBA and FDBA were used and other two groups were the positive and negative control group. Within the 2 consecutive months (2,4,6, and 8 weeks) every session, 5 rabbits were sacrificed and removed the calvaria bone for histologic and histomorphometric study. Data were analyzed using Friedman and Kruskal-Wallis and Wilcoxon tests. We utilized SPSS ver:20 software. Resul...
Sana Baghizadeh, Dental student, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad Univ... more Sana Baghizadeh, Dental student, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Email: sanabaghizadeh@ yahoo.com Background and Aim: The most common etiology of gingivitis is accumulation of bacterial plaque. Complete removal of microbial plaque by mechanical procedures is not possible in some cases; for example, aged and disabled patients might not be capable of removing the bacterial plaques properly. Also, chemical mouthwashes have some adverse effects. Therefore, finding a new treatment approach would be helpful. The global focus on the use of herbal medicine for treatment of different health conditions is on the rise. The present study aimed at searching and collecting scientific evidence regarding medicinal herbs to treat gingivitis and periodontitis. Materials and Methods: In this review, an electronic search of the literature was conducted through PubMed, Scopus, Google Scholar, and Wiley online library databases. Studies were considered...
Background Inflammatory bone resorption in periodontitis can lead to tooth loss. Systemic adminis... more Background Inflammatory bone resorption in periodontitis can lead to tooth loss. Systemic administration of bisphosphonates such as risedronate for preventing bone resorption can cause adverse effects. ALG and PLGA microparticles have been studied as drug delivery systems for sustained release of drugs. Therefore, the release pattern of risedronate from PLGA microparticles embedded with ALG was studied as a drug delivery system for sustained release of the drug, which can be used in local administrations. Methods Risedronate-containing PLGA microparticles were fabricated using double emulsion solvent evaporation technique. Ionic cross-linking method was used to fabricate risedronate-loaded ALG. Risedronate-containing PLGA microparticles were then coated with ALG. The calibration curve of risedronate was traced to measure EE and study the release pattern. SEM imaging was carried out, and cell toxicity was examined using MTT assay. Statistical analysis of data was carried out using SP...
Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering ... more Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations ofZingiber officinaleextract on proliferation ofStreptococcus mutansandStreptococcus sanguinisin vitro.Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛof each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛof each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as...
Journal of Dental Research, Dental Clinics, Dental Prospects, 2017
Background. Lateral ridge augmentation is conventionally accomplished by means of autogenous bone... more Background. Lateral ridge augmentation is conventionally accomplished by means of autogenous bone grafts. However, due to its complications, the application of autogenous bone graft substitutes, e.g. mineralized corticocancellous allograft, is ecommended. Methods. In the present study, twelve patients were included, with insufficient alveolar ridge widths in the designated sites for dental implant placement. During the primary surgery, mineralized corticocancellous block allografts were fixed in deficient sites with titanium screws and resorbable collagen membranes were used to cover the blocks. After a period of six months, a flap was raised and variations in ridge width values was measured. Finally, a micro-biopsy was obtained from the sites for histologic investigation prior to preparing them for subsequent implant placement. Results. All the applied blocks were incorporated into the underlying bone except for one. A statistically significant difference was seen between the avera...
Introduction: This study sought to assess the cytotoxic effects of nanoparticulate and micropart... more Introduction: This study sought to assess the cytotoxic effects of nanoparticulate and microparticulate calcium sodium phosphosilicate mouthwashes on human gingival fibroblasts (HGFs). Methods: This in vitro study was conducted on HGFs isolated and cultured in a 48-well plate containing standard culture medium for evaluation of four concentrations of the two mouthwashes at two time points plus a positive and a negative control group. The HGFs were exposed to 0.001, 0.01, 0.1 and 1 mg/mL concentrations of mouthwashes for 1 and 24 hours. Positive and negative control cells were exposed to saline and distilled water, respectively. Cell viability was assessed using the methyl thiazolyl tetrazolium (MTT) assay, and the number of viable cells was counted in triplicate using a cell counter after transfection (trypsin-EDTA 0.25%, 20 minutes) and exposure to trypan blue. The optical density (OD) values were read by ELISA reader and analyzed using the Kruskal-Wallis test. Results: Number of ...
Introduction: Mechanical debridement of diseased root surfaces produces a smear layer that encomp... more Introduction: Mechanical debridement of diseased root surfaces produces a smear layer that encompass microorganisms and residual cementum which may interfere with periodontal healing and regeneration of connective tissue attachment. Accordingly, this study aimed to determine impact of 940nm diode laser on adhesion of fibroblasts to root surface of extracted teeth from patients with chronic periodontitis. Methods: Twenty extracted single-rooted teeth with hopeless prognosis were collected and debrided with hand curettes. Afterward, two specimens were obtained from each tooth by splitting them with a sterile diamond disk. Samples were submerged in fibroblast suspension and randomly divided into two groups. Group A comprised of 20 specimens subjected to scaling and root planing only and group B included 20 specimens which received SRP and and 940 nm diode laser irradiation. The adhesion of fibroblasts was investigated by MTT and cell morphology was assessed with scanning electron micro...
Objective(s): The present study aimed to assess the in-vitro efficacy of nanoparticulate calcium ... more Objective(s): The present study aimed to assess the in-vitro efficacy of nanoparticulate calcium sodium phosphosilicate mouthwash in the obstruction of dentinal tubules.Materials and Methods: This in-vitro, study was conducted on 120 sections obtained from extracted human premolars, which were etched with citric acid for two minutes and rinsed with distilled water. Afterwards, the sections were randomly divided into two groups (60 per each) of nanoparticulate and regular mouthwash. In addition, each group was divided into six subgroups of 10. In the nanoparticulate mouthwash subgroups, one subgroup (n=10) was evaluated using scanning electron microscopy (SEM). The remaining five subgroups were immersed in artificial saliva for different time periods and inspected using SEM. The control subgroups were exposed to regular mouthwash. The diameters and number of the open dentinal tubules were evaluated and compared using two-way analysis of variance (ANOVA).Results: The mean number of th...
Statement of the Problem: Alveolar bone resorption associated with periodontal disease is a commo... more Statement of the Problem: Alveolar bone resorption associated with periodontal disease is a common finding and generally irreversible. It impairs mastication and causes esthetic problems for patients. Bisphosphonates are the most commonly used antiresorptive agents for bone diseases. Purpose: Considering the risk of bisphosphonate-related osteonecrosis of the jaw, this study aimed to assess the effect of 2% risedronate gel on calvarial bone defects in rabbits. Materials and Method: In this animal study, critical-size defects of 8mm were created in the calvaria of 20 New Zealand white rabbits. In group 1 (n=10), 2% risedronate gel was applied into the right side defect while the left side defect remained empty and served as control. In group 2 (n=10), placebo gel was applied into the right side defect, while the left side defect remained empty and served as control. Five rabbits in each group were sacrificed at 1month and the remaining five at 2 month, post-operatively, and tissue sa...
Introduction:Lateral ridge augmentations are traditionally performed using autogenous bone grafts... more Introduction:Lateral ridge augmentations are traditionally performed using autogenous bone grafts. However, the bone harvesting procedure, whether intra orally or extra orally, is accompanied by patient morbidity. So, applying bone substitute has been suggested. The aim of the present study was to compare the stability of implants in ridges augmented with mineralized cortical cancellous allograft blocks with implants in native bone using Resonance Frequency Analysis. Materials & Methods:In this clinical trial study, approved by ethical committee of Mashhad university of Medical Sciences, nine subjects with insufficient ridge width were selected. Their ridges were augmented with mineralized cortical cancellous allograft blocks. After 6 months, implants were inserted. In control group, 9 subjects with sufficient ridge width were selected. Implants were inserted in the same place as the test group. ISQ was used for measurement of implants stability on third and sixth month after implan...
Introduction: Recently, the management of dentin hypersensitivity by lasers has gained special at... more Introduction: Recently, the management of dentin hypersensitivity by lasers has gained special attention. This study aimed to assess and compare the efficacy of the 980 nm diode, Nd:YAG and Er:YAG lasers accompanied by fluoride in dentinal tubule obstruction. Methods: Twenty sound single-rooted human teeth were used for this invitro study. Forty dentinal discs were prepared of the roots and etched with 6% citric acid. One layer of fluoride varnish was applied over their surface. The sections were randomly allocated into 4 groups. The control group received no laser irradiation. Group 2 underwent 980 nm diode laser irradiation with 0.5 W power. Group 3 underwent Nd:YAG laser irradiation with 0.5 W power and group 4 underwent Er:YAG laser irradiation with 0.5 W power. All samples were then inspected under a scanning electron microscope, and the number of obstructed dentinal tubules and the diameter of open dentinal tubules in the field were determined. One-way ANOVA and Tukey’s test w...
Uploads