Occurrence of Bacterial Haemorrhagicsepticaemia (BHS) has been recorded in juvenile / mature Clar... more Occurrence of Bacterial Haemorrhagicsepticaemia (BHS) has been recorded in juvenile / mature Clarias batrachus during the summer months (April-July) of the years 2004-2006. The symptomatic fishes were exhibiting distended abdomen, haemorrhages, necrotization and ulceration on the general body surface, lethargic behaviour, low appetite, erosion of fins, swollen vents, presence of ascetic fluid in the abdomen and empty intestine filled with exudate fluid. Bacteriological examination revealed the appearance of colonies of Pseudomonas fluorescens (PF) in the form of convex / flat rounded with smooth edges and yellowish to fluorescent green on to agar medium. They proliferate at 30 ± 5 0 C temperature but did not develop at 45 0 C. Out of fifteen isolates PF4 was more virulent. Biochemical characterization of the PF isolates explicated that they were oxidative in nature, oxidase & catalase positive, gelatin, nitrate, arginin, and citrate degradative, mannitol, inositol, sorbitol and arbinose positive but lysine, ornithin, urease, H2S, indole and MR-VP responses negative. Experimental infection trial revealed 100% mortality in CB challenged with bacterial suspension (PF4) containing 6.1 x 10 5 , 6.1 x 10 4 , 6.1 x 10 3 cfu/ml and LD50 value of this isolate was enumerated to 6.1x10 3.5 cfu/ml fish-1 in case of CB.
Indian journal of biotechnology and pharmaceutical research, 2023
Influence of immunomodulators (levamisole, vitamin-E and selenium) on the lysozyme level has been... more Influence of immunomodulators (levamisole, vitamin-E and selenium) on the lysozyme level has been evaluated in Clarias batrachus challenging with levamisole injection (@ 100 µg & 500 µg ml-1 fish-1), immersion (@ 1.25 & 2.50 ppm), feed supplementation (@ 5-10% of body weight of fish) with pellated feed of vitamin-E (@ 800 mg & 1600 mg Kg-1 feed), selenium (@ 1.8 mg & 3.6 mg Kg-1 feed) and the mixture of levamisole (@75 & 150), vitamin-E (@800 & 1600) and selenium (@1.8 & 3.6) mg Kg-1 feed. Determination of serum lysozyme concentration of controlled and challenged CB has been accomplished by lysate plate method in which wells were developed in agarose plate, loaded with serum samples, incubated at 37 0 C in humid chamber and diameters of lysed zones were measured and concentration of serum lysozyme was determine following regression equation. Lysozyme concentration has been found to be elevated in HLD1, HLD2; HED1, HED2, MD1, MD2, HMD1, HMD2, OMD1 and OMD2 treated CB while in other groups this level declined.
Occurrence of Bacterial Haemorrhagicsepticaemia (BHS) has been recorded in juvenile / mature Clar... more Occurrence of Bacterial Haemorrhagicsepticaemia (BHS) has been recorded in juvenile / mature Clarias batrachus during the summer months (April-July) of the years 2004-2006. The symptomatic fishes were exhibiting distended abdomen, haemorrhages, necrotization and ulceration on the general body surface, lethargic behaviour, low appetite, erosion of fins, swollen vents, presence of ascetic fluid in the abdomen and empty intestine filled with exudate fluid. Bacteriological examination revealed the appearance of colonies of Pseudomonas fluorescens (PF) in the form of convex / flat rounded with smooth edges and yellowish to fluorescent green on to agar medium. They proliferate at 30 ± 5 0 C temperature but did not develop at 45 0 C. Out of fifteen isolates PF4 was more virulent. Biochemical characterization of the PF isolates explicated that they were oxidative in nature, oxidase & catalase positive, gelatin, nitrate, arginin, and citrate degradative, mannitol, inositol, sorbitol and arbinose positive but lysine, ornithin, urease, H2S, indole and MR-VP responses negative. Experimental infection trial revealed 100% mortality in CB challenged with bacterial suspension (PF4) containing 6.1 x 10 5 , 6.1 x 10 4 , 6.1 x 10 3 cfu/ml and LD50 value of this isolate was enumerated to 6.1x10 3.5 cfu/ml fish-1 in case of CB.
Indian journal of biotechnology and pharmaceutical research, 2023
Influence of immunomodulators (levamisole, vitamin-E and selenium) on the lysozyme level has been... more Influence of immunomodulators (levamisole, vitamin-E and selenium) on the lysozyme level has been evaluated in Clarias batrachus challenging with levamisole injection (@ 100 µg & 500 µg ml-1 fish-1), immersion (@ 1.25 & 2.50 ppm), feed supplementation (@ 5-10% of body weight of fish) with pellated feed of vitamin-E (@ 800 mg & 1600 mg Kg-1 feed), selenium (@ 1.8 mg & 3.6 mg Kg-1 feed) and the mixture of levamisole (@75 & 150), vitamin-E (@800 & 1600) and selenium (@1.8 & 3.6) mg Kg-1 feed. Determination of serum lysozyme concentration of controlled and challenged CB has been accomplished by lysate plate method in which wells were developed in agarose plate, loaded with serum samples, incubated at 37 0 C in humid chamber and diameters of lysed zones were measured and concentration of serum lysozyme was determine following regression equation. Lysozyme concentration has been found to be elevated in HLD1, HLD2; HED1, HED2, MD1, MD2, HMD1, HMD2, OMD1 and OMD2 treated CB while in other groups this level declined.
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