https://www.researchgate.net/profile/Hesham_Mustafa Supervisors: Member of editorial Board of the International Journal of Clinical Endocrinology and Metabolism and Member of Editorial Board of the Peertechz Journal of Cytology and Pathology Phone: +966566764762 Address: Compound Diar Eltameer (1)- Gamal Abdel Nasser Road - Building 19 second floor Apartment 24
Hesham N.Mustafa is one of the World's Top 2% Scientist 2024 according to Stanford University-Els... more Hesham N.Mustafa is one of the World's Top 2% Scientist 2024 according to Stanford University-Elsevier ranking
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I hav... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I hav... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I h... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I h... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I h... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
The aim of the present study was to investigate the histological structure of the fleshy muscle-b... more The aim of the present study was to investigate the histological structure of the fleshy muscle-bone interface in selected limb muscles in man, as compared to that of the enthesis, in an attempt to clarify the way muscle fibers transmit their contractile force to adjacent bone. The muscle specimens were taken from biceps and tendocalcaneus as examples for the tendon-bone attachment (enthesis), from external intercostal, brachioradialis, and external oblique muscles as examples for the linear fleshy attachment, and from infraspinatus and brachialis as examples for the fleshy attachment over a wide area. The muscle-bone interface specimens were collected form six formalin-fixed dissecting room elderly male cadavers with no gross pathology. The whole muscle-bone interface was extracted so that each specimen included the muscle and the underlying bone tissues. The specimens were fixed in 10% neutral buffered formol saline for one week, and then decalcified with 10% EDTA for about 4-6 weeks. Dehydrated in ascending grades of alcohols, cleared in xylol, and embedded in paraffin wax. Serial sections were cut at 8-µm thickness and stained with Haematoxylin and eosin, and Masson's trichrome. In the present work, it was found that tendon-bone attachment of either biceps brachii or tendocalcaneus was formed of four zones; zone 1 (Z1) of dense connective tissue, zone 2 (Z2) of fibrocartilage, zone 3 (Z3) of calcified fibrocartilage, and zone 4 (Z4) of compact bone. Serrated basophilic line "tidemark" was usually seen between fibrocartilage and calcified fibrocartilage zones. Moreover, differences in the distribution and population of chondrocytes occurred between zone 2 (Z2) and zone 3 (Z3). On the other hand, the muscle-bone interface of brachialis, infraspinatus, brachioradialis, and external intercostal muscles was noticed to be formed of three zones; zone 1 (Z1) of skeletal muscle tissue, zone 2 (Z2) of dense connective tissue, and zone 3 (Z3) of compact bone. The dense connective tissue zone interposed between the skele [...]
Hepatitis B virus (HBV)infection is a serious global health problem,with 2 billion people infecte... more Hepatitis B virus (HBV)infection is a serious global health problem,with 2 billion people infected worldwide,and 350 million suffering from chronic HBV infection.Being the 10th leading cause of death worldwide,HBV infections result in 0.5 to 1.2 million deaths per year caused by chronic hepatitis,cirrhosis,and hepatocellular carcinoma.Besides,hepatitis B is a major health problem in Egypt.It was reported that HBV was more prevalent in Upper Egypt (11.7)than in Lower Egypt (8.8). The aim of the present work is to reveal the prevalence of HBV worldwide and in Egypt and to discuss updated clinical issues in the diagnosis and modern modalities of treatment.It was concluded that hopefully,the advent of universal infant vaccination,treatment of all forms of chronic HBV disease,and new screening methods,as well as treatment modalities for HBV induced cirrhosis and liver cancer in the next decade,will enable us to markedly decrease the incidence and prevalence of this worldwide endemic disease
Background Quercetin, a naturally occurring flavonoid known for its potent antioxidant properties... more Background Quercetin, a naturally occurring flavonoid known for its potent antioxidant properties, has been investigated for its potential in counteracting the harmful effects of lead (Pb) toxicity, which induces apoptosis and oxidative damage in various human tissues. This study aims to assess the reparative effects of quercetin on lead-induced testicular damage. Methods Four groups, each comprising ten adult male albino rats, were randomly assigned as follows: Quercetin group, Pb group, Pb + Quercetin group, and control group. All treatments were administered orally via gavage daily for a duration of 30 days. Evaluation of sex hormone levels (serum testosterone, FSH, and LH), cytokines and inflammatory mediators (IL-1β, TNF-α, MCP-1), lead concentration, oxidative and antioxidant stress markers (superoxide anion [O2−], MDA, SOD, CAT, GSH), and sperm characteristics were carried out. Results The results demonstrated a significant decline in sex hormones and antioxidants, accompanie...
Background: The aim of the present work was to provide evidence about the anatomical variations a... more Background: The aim of the present work was to provide evidence about the anatomical variations as regard the origin, distribution, and branching pattern of the musculocutaneous nerve (MCN). Materials and methods: Brachial plexus was dissected in 40 upper limbs of 20 male adult cadavers. The pattern of the musculocutaneous nerve was photographed by a digital camera. Results: The location and length of the nerve branches between left and right arms were recorded and statistically analyzed. In (90%) of specimens the MCN originates from the lateral cord of the brachial plexus, in (5%) it arose from the median nerve (MN), while in the remaining (5%) specimen, it was absent. The musculocutaneous nerve pierced the coracobrachialis muscle in 90% of specimens, and in the remaining (10%) did not pierce it. The motor branches to biceps brachii muscle were categorized into: Type 1 (90%): one branch that divides to supply the two heads of biceps; Type 2 (5%): double branches, innervating each head of biceps separately. The motor branches to brachialis muscle were categorized into: Type 1 (82.9%): one branch; Type 2 (14.2%): double branches and Type 3 (2.9%): three branches that innervating brachialis muscle. Communications between the MCN and the MN were observed in 35% of specimens. Conclusions: The knowledge of the common and uncommon musculocutaneous nerve variations is important especially to the surgeons for carrying out surgical procedures in axilla and arm.
Aluminum (Al) is a neurotoxic substance which has played an important role in the etiology, patho... more Aluminum (Al) is a neurotoxic substance which has played an important role in the etiology, pathogenesis, and development of amyloid-β (Aβ) plaques. This study was carried out to evaluate the neuroprotective effect of aqueous cinnamon extract against aluminum chloride (AlCl 3)induced Alzheimer's disease. Forty adult male albino rats, randomly divided into four equal groups. Control group; ACE200 group administered aqueous cinnamon extract (ACE) orally; AlCl 3 group received daily intraperitoneal (i.p.) injection of AlCl 3 for 60 days to induce neurotoxicity and AlCl 3 + ACE200 group received a combination of AlCl 3 and ACE in the same dose and route as previous groups. Aluminum administration significantly enhanced the memory impairment and the Aβ formation in the rat model. The cerebellum exhibited a significant reduced number of Purkinje cells, marked decrease in the density of dendritic arborization and prominent perineuronal spaces in the molecular layer. There was loss of dendritic spines, neurofibrillary degeneration, and appearance of neuritic plaques. Concomitant administration of AlCl 3 and ACE displayed an observable protection against these changes with progressive improvement in memory and intellectual performance. In conclusion, ACE may play a protective role against formation of amyloid-β plaques in cerebellum.
Hesham N.Mustafa is one of the World's Top 2% Scientist 2024 according to Stanford University-Els... more Hesham N.Mustafa is one of the World's Top 2% Scientist 2024 according to Stanford University-Elsevier ranking
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I hav... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I hav... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I h... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I h... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I h... more 🌟 Honored to Be Among the World's Top 2% Scientists for 2024 🌟
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
The aim of the present study was to investigate the histological structure of the fleshy muscle-b... more The aim of the present study was to investigate the histological structure of the fleshy muscle-bone interface in selected limb muscles in man, as compared to that of the enthesis, in an attempt to clarify the way muscle fibers transmit their contractile force to adjacent bone. The muscle specimens were taken from biceps and tendocalcaneus as examples for the tendon-bone attachment (enthesis), from external intercostal, brachioradialis, and external oblique muscles as examples for the linear fleshy attachment, and from infraspinatus and brachialis as examples for the fleshy attachment over a wide area. The muscle-bone interface specimens were collected form six formalin-fixed dissecting room elderly male cadavers with no gross pathology. The whole muscle-bone interface was extracted so that each specimen included the muscle and the underlying bone tissues. The specimens were fixed in 10% neutral buffered formol saline for one week, and then decalcified with 10% EDTA for about 4-6 weeks. Dehydrated in ascending grades of alcohols, cleared in xylol, and embedded in paraffin wax. Serial sections were cut at 8-µm thickness and stained with Haematoxylin and eosin, and Masson's trichrome. In the present work, it was found that tendon-bone attachment of either biceps brachii or tendocalcaneus was formed of four zones; zone 1 (Z1) of dense connective tissue, zone 2 (Z2) of fibrocartilage, zone 3 (Z3) of calcified fibrocartilage, and zone 4 (Z4) of compact bone. Serrated basophilic line "tidemark" was usually seen between fibrocartilage and calcified fibrocartilage zones. Moreover, differences in the distribution and population of chondrocytes occurred between zone 2 (Z2) and zone 3 (Z3). On the other hand, the muscle-bone interface of brachialis, infraspinatus, brachioradialis, and external intercostal muscles was noticed to be formed of three zones; zone 1 (Z1) of skeletal muscle tissue, zone 2 (Z2) of dense connective tissue, and zone 3 (Z3) of compact bone. The dense connective tissue zone interposed between the skele [...]
Hepatitis B virus (HBV)infection is a serious global health problem,with 2 billion people infecte... more Hepatitis B virus (HBV)infection is a serious global health problem,with 2 billion people infected worldwide,and 350 million suffering from chronic HBV infection.Being the 10th leading cause of death worldwide,HBV infections result in 0.5 to 1.2 million deaths per year caused by chronic hepatitis,cirrhosis,and hepatocellular carcinoma.Besides,hepatitis B is a major health problem in Egypt.It was reported that HBV was more prevalent in Upper Egypt (11.7)than in Lower Egypt (8.8). The aim of the present work is to reveal the prevalence of HBV worldwide and in Egypt and to discuss updated clinical issues in the diagnosis and modern modalities of treatment.It was concluded that hopefully,the advent of universal infant vaccination,treatment of all forms of chronic HBV disease,and new screening methods,as well as treatment modalities for HBV induced cirrhosis and liver cancer in the next decade,will enable us to markedly decrease the incidence and prevalence of this worldwide endemic disease
Background Quercetin, a naturally occurring flavonoid known for its potent antioxidant properties... more Background Quercetin, a naturally occurring flavonoid known for its potent antioxidant properties, has been investigated for its potential in counteracting the harmful effects of lead (Pb) toxicity, which induces apoptosis and oxidative damage in various human tissues. This study aims to assess the reparative effects of quercetin on lead-induced testicular damage. Methods Four groups, each comprising ten adult male albino rats, were randomly assigned as follows: Quercetin group, Pb group, Pb + Quercetin group, and control group. All treatments were administered orally via gavage daily for a duration of 30 days. Evaluation of sex hormone levels (serum testosterone, FSH, and LH), cytokines and inflammatory mediators (IL-1β, TNF-α, MCP-1), lead concentration, oxidative and antioxidant stress markers (superoxide anion [O2−], MDA, SOD, CAT, GSH), and sperm characteristics were carried out. Results The results demonstrated a significant decline in sex hormones and antioxidants, accompanie...
Background: The aim of the present work was to provide evidence about the anatomical variations a... more Background: The aim of the present work was to provide evidence about the anatomical variations as regard the origin, distribution, and branching pattern of the musculocutaneous nerve (MCN). Materials and methods: Brachial plexus was dissected in 40 upper limbs of 20 male adult cadavers. The pattern of the musculocutaneous nerve was photographed by a digital camera. Results: The location and length of the nerve branches between left and right arms were recorded and statistically analyzed. In (90%) of specimens the MCN originates from the lateral cord of the brachial plexus, in (5%) it arose from the median nerve (MN), while in the remaining (5%) specimen, it was absent. The musculocutaneous nerve pierced the coracobrachialis muscle in 90% of specimens, and in the remaining (10%) did not pierce it. The motor branches to biceps brachii muscle were categorized into: Type 1 (90%): one branch that divides to supply the two heads of biceps; Type 2 (5%): double branches, innervating each head of biceps separately. The motor branches to brachialis muscle were categorized into: Type 1 (82.9%): one branch; Type 2 (14.2%): double branches and Type 3 (2.9%): three branches that innervating brachialis muscle. Communications between the MCN and the MN were observed in 35% of specimens. Conclusions: The knowledge of the common and uncommon musculocutaneous nerve variations is important especially to the surgeons for carrying out surgical procedures in axilla and arm.
Aluminum (Al) is a neurotoxic substance which has played an important role in the etiology, patho... more Aluminum (Al) is a neurotoxic substance which has played an important role in the etiology, pathogenesis, and development of amyloid-β (Aβ) plaques. This study was carried out to evaluate the neuroprotective effect of aqueous cinnamon extract against aluminum chloride (AlCl 3)induced Alzheimer's disease. Forty adult male albino rats, randomly divided into four equal groups. Control group; ACE200 group administered aqueous cinnamon extract (ACE) orally; AlCl 3 group received daily intraperitoneal (i.p.) injection of AlCl 3 for 60 days to induce neurotoxicity and AlCl 3 + ACE200 group received a combination of AlCl 3 and ACE in the same dose and route as previous groups. Aluminum administration significantly enhanced the memory impairment and the Aβ formation in the rat model. The cerebellum exhibited a significant reduced number of Purkinje cells, marked decrease in the density of dendritic arborization and prominent perineuronal spaces in the molecular layer. There was loss of dendritic spines, neurofibrillary degeneration, and appearance of neuritic plaques. Concomitant administration of AlCl 3 and ACE displayed an observable protection against these changes with progressive improvement in memory and intellectual performance. In conclusion, ACE may play a protective role against formation of amyloid-β plaques in cerebellum.
Background Aluminum (AL) is a neurotoxic substance that plays an important role in the etiology, ... more Background Aluminum (AL) is a neurotoxic substance that plays an important role in the etiology, pathogenesis and development of Amyloid-β plaques. This study was carried out to evaluate neuroprotective effect of Aqueous Cinnamon Extract against aluminum chloride (ALCl3)-induced Alzheimer's disease. Materials and methods 40 adult male albino rats, randomly divided into four equal groups. Group I: control; Group 2 were administered 200 mg/kg b. w./day aqueous cinnamon extract (ACE) via oral tube; Group 3: received daily intraperitoneal (i.p.) injection of ALCl3 (100 mg/kg body weight) for 60 days to induce neurotoxicity; Group 4 received a combination of ALCl3 and ACE in the same dose and route as group 2 & 3. Results AL administration significantly enhanced the memory impairment, Aβ burden and the cerebellum exhibited significant reduction in the number of Purkinje cells, marked decrease in the density of dendritic arborization and prominent perineuronal spaces in the molecular layer around basket and stellate cells, loss of dendritic spines, neurofibrillary degeneration and appearance of neuritic plaques. Concomitant administration of ACE with AL displayed an observable protection against these changes with progressive improvement in memory and in intellectual performance. It has also been suggested that the loss of astrocyte functions may precede neurodegeneration and that aluminum could be a contributing factor [1]. Astrocytes are the principal target of the toxic action of aluminum [2] and it can cause astrocyte death through apoptosis [3]. Conclusion: ACE may be considered an efficacious therapeutic strategy to alleviate Amyloid-β plaques. It is recommended that AL not be used in cooking utensils, water tubes, and to control occupational exposure.
Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its cli... more Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group. CoQ10 and L-carnitine treatment orally started 5days before a single dose of 10mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1 β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.
KEYWORDS: Cardiotoxicity; CoQ10 and L-carnitine; Dox; Vimentin; eNOS
20th International Conference on Biochemistry, Biophysics and Molecular Biology.
Doxorubicin (DO... more 20th International Conference on Biochemistry, Biophysics and Molecular Biology.
Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group. CoQ10 and L-carnitine treatment orally started 5days before a single dose of 10mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1 β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.
KEYWORDS: Cardiotoxicity; CoQ10 and L-carnitine; Dox; Vimentin; eNOS
BACKGROUND: The current study aimed to elucidate the protective role of combined fenugreek and a-... more BACKGROUND: The current study aimed to elucidate the protective role of combined fenugreek and a-tocopherol against cadmium induced histopathological changes in the testes. MATERIALS-METHODS: Thirty adult male albino rats divided into three equal groups 10 rats each. Group I is the control group. Group II received 5 mg/kg/ day cadmium chloride. Group III received 5 mg/kg/day cadmium chloride and 150 mg/kg/day fenugreek and 100 mg/kg/ day of a-tocopherol. The treatment of all groups was done by oral gavage for 60 consecutive days. The testes were removed and subjected to histopathological and ultrastructure study. RESULTS: Rats exposed to cadmium showed severe histopathological changes in the testicular spermatogenic series, many vacuoles and multinucleated giant cells. Treatment with fenugreek and a-tocopherol partially improved the morphological changes, reduced tissue damage and rebuilt of the spermatogonia layer. CONCLUSIONS: Fenugreek and a-tocopherol might represent a promising medicinal combination to ameliorate the toxic effects of cadmium exposure.
Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed t... more Diabetic patients frequently suffer from non-alcoholic steatohepatitis. The current study aimed to investigate the role of curcumin and the response of hepatic stellate cells in streptozotocin (STZ)-induced hepatic damage. Sixty male rats were divided into three groups. The normal control injected with a citrate buffer vehicle and the diabetic control group which was injected intraperitoneally (IP) with a single-dose of streptozotocin (50 mg/kg body weight) and a diabetic group was treated with an oral dose of curcumin at 80 mg/kg body weight daily for 60 days. Results. Curcumin effectively counteracts oxidative stress-mediated hepatic damage and improves biochemical parameters. Alpha-smooth muscle actin (α-SMA) was significantly reduced, and insulin antibodies showed strong positive immunoreactivity with curcumin administration. These results optimistically demonstrate the potential use of curcumin, which is attributed to its antiradical/ antioxidant activities and its potential β-cell regenerative properties. Also, it has the capability to encourage the trans-differentiation of hepatic stellate cells into insulin-producing cells for a period of time. In addition, as it is an anti-fibrotic mediator that inhibits hepatic stellate cell activation and the transition to myofibroblast-like cells, this suggests the possibility of considering curcumin's novel therapeutic effects in reducing hepatic dysfunction in diabetic patients. Body and liver weight of different groups. The mean is given in columns, and error bars represent the standard deviation (SD). Fig. 2 Plasma glucose and insulin of different groups. The mean is given in columns, and error bars represent the standard deviation (SD). Fig. 3 Biochemical parameters of different groups. The mean is given in columns, and error bars represent the standard deviation (SD).
Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its cli... more Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10 mg/kg); CoQ10 group (200 mg/kg); L-carnitine group (100 mg/kg); DOX + CoQ10 group; DOX + L-carnitine group. CoQ10 and L-carnitine treatment orally started 5 days before a single dose of 10 mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10 days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. In conclusion, CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.
BACKGROUND:
The current article aims to explore the protective potentials of α-tocopherol alone ... more BACKGROUND:
The current article aims to explore the protective potentials of α-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex. MATERIALS AND METHODS:
Forty rats were divided into four groups (n=10). Group 1 was the control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP). RESULTS:
The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of α-tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups. CONCLUSIONS:
Although both α-tocopherol and the combination of allicin and vit. B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, α-tocopherol showed more protective potential. KEYWORDS:
Tissue And Cell "Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in Do... more Tissue And Cell "Protective role of CoQ10 or L-carnitine on the integrity of the myocardium in Doxorubicin induced toxicity. "
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Papers by Prof. Dr. Hesham N Mustafa 🩺
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
I am thrilled to share that I have been recognized as one of the World's Top 2% Scientists for 2024, according to the prestigious Stanford University-Elsevier ranking.
This recognition reflects the collective efforts and support of my colleagues, mentors, students, and collaborators who have been part of my journey. It also serves as a reminder of the importance of research and its impact on society.
I am deeply grateful to everyone for providing a platform to pursue meaningful research and to the global scientific community for its relentless pursuit of knowledge and innovation.
This milestone motivates me to continue striving for excellence and to contribute to advancing biomedical research.
Thank you to everyone who has supported my work, and I look forward to what the future holds!
KEYWORDS:
Cardiotoxicity; CoQ10 and L-carnitine; Dox; Vimentin; eNOS
Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group. CoQ10 and L-carnitine treatment orally started 5days before a single dose of 10mg/kg DOX that injected intraperitoneally (IP) then the treatment continued for 10days. At the end of the study, serum biochemical parameters of cardiac damage, oxidative stress indices, and histopathological changes were investigated. CoQ10 or L-carnitine showed a noticeable effects in improving cardiac functions evidenced reducing serum enzymes as serum interleukin-1 beta (IL-1 β), tumor necrosis factor alpha (TNF-α), leptin, lactate dehydrogenase (LDH), Cardiotrophin-1, Troponin-I and Troponin-T. Also, alleviate oxidative stress, decrease of cardiac Malondialdehyde (MDA), Nitric oxide (NO) and restoring cardiac reduced glutathione levels to normal levels. Both corrected the cardiac alterations histologically and ultrastructurally. With a visible improvements in α-SMA, vimentin and eNOS immunohistochemical markers. CoQ10 or L-carnitine supplementation improves the functional and structural integrity of the myocardium.
KEYWORDS:
Cardiotoxicity; CoQ10 and L-carnitine; Dox; Vimentin; eNOS
The current article aims to explore the protective potentials of α-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex.
MATERIALS AND METHODS:
Forty rats were divided into four groups (n=10). Group 1 was the control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP).
RESULTS:
The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of α-tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.
CONCLUSIONS:
Although both α-tocopherol and the combination of allicin and vit. B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, α-tocopherol showed more protective potential.
KEYWORDS:
Allicin; Astrocytes; GFAP; Myelin Figure; Oligodendrocyte; Purkinje cells