ST239-MRSA-III is probably the oldest truly pandemic MRSA strain, circulating in many countries s... more ST239-MRSA-III is probably the oldest truly pandemic MRSA strain, circulating in many countries since the 1970s. It is still frequently isolated in some parts of the world although it has been replaced by other MRSA strains in, e.g., most of Europe. Previous genotyping work (Harris et al., 2010; Castillo-Ramírez et al., 2012) suggested a split in geographically defined clades. In the present study, a collection of 184 ST239-MRSA-III isolates, mainly from countries not covered by the previous studies were characterized using two DNA microarrays (i) targeting an extensive range of typing markers, virulence and resistance genes and (ii) a SCC subtyping array. Thirty additional isolates underwent whole-genome sequencing (WGS) and, together with published WGS data for 215 ST239-MRSA-III isolates, were analyzed using analysis for comparison with the microarray data and with special regard to variation within SCC elements. This permitted the assignment of isolates and sequences to 39 diffe...
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, Jan 27, 2016
CC22-MRSA-IV, UK-EMRSA-15/Barnim EMRSA, is a common and pandemic strain of MRSA that has mainly b... more CC22-MRSA-IV, UK-EMRSA-15/Barnim EMRSA, is a common and pandemic strain of MRSA that has mainly been found in Western Europe but also in other parts of the world including some Gulf countries. One suspected case of an infection with this strain of a patient who was admitted to the surgical unit in Riyadh, Kingdom of Saudi Arabia (KSA) was investigated in order to check whether this strain has reached KSA. Beside the index isolate, forty-six additional isolates of CC22-MRSA-IV from patients from KSA, Abu Dhabi, Kuwait and from Germany, with Middle Eastern travel history, were characterized by microarray hybridization. The study revealed a regional presence of as much as six distinct "strains" of CC22-MRSA-IV that can be distinguished based on carriage of SCCmec IV subtypes and virulence factors. No true UK-EMRSA-15/Barnim EMRSA was identified in Riyadh with all suspected isolates from there being assigned to other, albeit related strains. However, this strain was identified...
Objectives: To determine the trafficking of methicillin-resistant staphylococci between the hospi... more Objectives: To determine the trafficking of methicillin-resistant staphylococci between the hospital and community as well as the occurrence of co-colonization with vancomycin-resistant enterococci (VRE). Subjects and Methods: From November 2005 to April 2006, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus (MRCoNS)-positive patients at the Salmaniya Medical Complex, Bahrain were assessed for VRE co-colonization. Characterization of vancomycin resistance genotype by PCR was carried out. Close family contacts were screened for MRSA and pulsed-field gel electrophoresis (PFGE) analysis of MRSA isolates from patient-family member pairs was conducted. Results: One hundred and eighty-two patients (93 MRSA; 89 MRCoNS) and 356 family members were enrolled. Seven MRSA and 41 MRCoNS strains were isolated from the family members. PFGE analysis revealed the presence of variants of a single MRSA clone among patients and their relativ...
Eighteen methicillin-resistant Staphylococcus aureus (MRSA) samples isolated from patients and th... more Eighteen methicillin-resistant Staphylococcus aureus (MRSA) samples isolated from patients and the environment in an intensive care unit (ICU) during a routine surveillance were tested for antimicrobial resistance and typed by pulsed-field gel electrophoresis. Three pulsed-field patterns were observed. Sixteen were ciprofloxacin resistant and had identical pulsed-field patterns. The results suggested that a ciprofloxacin-resistant MRSA clone had contaminated the environment and spread among patients. This study demonstrates the application of infection control surveillance combined with strain typing in detecting MRSA colonization in the ICU where it was not known to exist.
ST239-MRSA-III is probably the oldest truly pandemic MRSA strain, circulating in many countries s... more ST239-MRSA-III is probably the oldest truly pandemic MRSA strain, circulating in many countries since the 1970s. It is still frequently isolated in some parts of the world although it has been replaced by other MRSA strains in, e.g., most of Europe. Previous genotyping work (Harris et al., 2010; Castillo-Ramírez et al., 2012) suggested a split in geographically defined clades. In the present study, a collection of 184 ST239-MRSA-III isolates, mainly from countries not covered by the previous studies were characterized using two DNA microarrays (i) targeting an extensive range of typing markers, virulence and resistance genes and (ii) a SCC subtyping array. Thirty additional isolates underwent whole-genome sequencing (WGS) and, together with published WGS data for 215 ST239-MRSA-III isolates, were analyzed using analysis for comparison with the microarray data and with special regard to variation within SCC elements. This permitted the assignment of isolates and sequences to 39 diffe...
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, Jan 27, 2016
CC22-MRSA-IV, UK-EMRSA-15/Barnim EMRSA, is a common and pandemic strain of MRSA that has mainly b... more CC22-MRSA-IV, UK-EMRSA-15/Barnim EMRSA, is a common and pandemic strain of MRSA that has mainly been found in Western Europe but also in other parts of the world including some Gulf countries. One suspected case of an infection with this strain of a patient who was admitted to the surgical unit in Riyadh, Kingdom of Saudi Arabia (KSA) was investigated in order to check whether this strain has reached KSA. Beside the index isolate, forty-six additional isolates of CC22-MRSA-IV from patients from KSA, Abu Dhabi, Kuwait and from Germany, with Middle Eastern travel history, were characterized by microarray hybridization. The study revealed a regional presence of as much as six distinct "strains" of CC22-MRSA-IV that can be distinguished based on carriage of SCCmec IV subtypes and virulence factors. No true UK-EMRSA-15/Barnim EMRSA was identified in Riyadh with all suspected isolates from there being assigned to other, albeit related strains. However, this strain was identified...
Objectives: To determine the trafficking of methicillin-resistant staphylococci between the hospi... more Objectives: To determine the trafficking of methicillin-resistant staphylococci between the hospital and community as well as the occurrence of co-colonization with vancomycin-resistant enterococci (VRE). Subjects and Methods: From November 2005 to April 2006, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus (MRCoNS)-positive patients at the Salmaniya Medical Complex, Bahrain were assessed for VRE co-colonization. Characterization of vancomycin resistance genotype by PCR was carried out. Close family contacts were screened for MRSA and pulsed-field gel electrophoresis (PFGE) analysis of MRSA isolates from patient-family member pairs was conducted. Results: One hundred and eighty-two patients (93 MRSA; 89 MRCoNS) and 356 family members were enrolled. Seven MRSA and 41 MRCoNS strains were isolated from the family members. PFGE analysis revealed the presence of variants of a single MRSA clone among patients and their relativ...
Eighteen methicillin-resistant Staphylococcus aureus (MRSA) samples isolated from patients and th... more Eighteen methicillin-resistant Staphylococcus aureus (MRSA) samples isolated from patients and the environment in an intensive care unit (ICU) during a routine surveillance were tested for antimicrobial resistance and typed by pulsed-field gel electrophoresis. Three pulsed-field patterns were observed. Sixteen were ciprofloxacin resistant and had identical pulsed-field patterns. The results suggested that a ciprofloxacin-resistant MRSA clone had contaminated the environment and spread among patients. This study demonstrates the application of infection control surveillance combined with strain typing in detecting MRSA colonization in the ICU where it was not known to exist.
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