An optimized circular polymerase extension reaction-based method for functional analysis of SARS-CoV-2

Abstract Background Reverse genetics systems have been crucial for studying specific viral genes and their relevance in the virus lifecycle, and become important tools for the rational attenuation of viruses and thereby for vaccine design. Recent rapid progress has been made in the establishment of...

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Bibliographic Details
Main Authors: GuanQun Liu, Michaela U. Gack
Format: Article
Language:English
Published: BMC 2023-04-01
Series:Virology Journal
Subjects:
Online Access:https://doi.org/10.1186/s12985-023-02025-y