Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology, 2020
This report describes the clinical and surgical approach of a complex odontoma with periapical in... more This report describes the clinical and surgical approach of a complex odontoma with periapical involvement implementing a new bioceramic cement for retrobturation. The patient presented for routine consultation with slight dental mobility in the anterosuperior region. On clinical, radiographic and computed tomography examinations, bone rarefaction was observed in the apical region of the elements 11 and 12, with density higher than bone and similar to teeth, 2.5 cm in diameter, external root resorption of the elements involved, and pulp necrosis of the 12. Conventional endodontic treatment was performed. In a second intervention, a surgical excision of the lesion was performed, showing a mass with clinical characteristics of complex odontoma. Element 12 was retrobturated with Aggregate Pentaoxide Mineral Bioceramic Cement (5 MO). Histopathological examination confirmed the diagnosis of complex odontoma. On follow-up there were no reports of symptoms and the clinical signs were satis...
Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South A... more Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell prol...
Objective: Covid-19 is a highly infectious disease; it is transmitted by patients’ secretions and... more Objective: Covid-19 is a highly infectious disease; it is transmitted by patients’ secretions and respiratory droplets SARS-CoV-2 was detected in patients saliva making dental care professionals more susceptible for contamination and the patient less safe while seeking the dental treatment. Methodology: This quantitative study was elaborated through a questionnaire for patients who frequently seek dental care in private and publica dental care units. A total of 205 over-18 patients were included, those who left one or more questions without answer were excluded. Participants were able to refuse to answer any question or to participate in the questionnaire at all. The questionnaire was composed of five questions. Participants were divided into two groups: Group 1: private dental offices patients (n=98); Group 2: public dental clinic patients (n=107). Results: there was a significant difference between groups 1 and 2, in which 50% of group 1 feel safe while seeking dental care during ...
Introduction: This study aimed to evaluate the effectiveness of N-acetylcysteine (NAC), photodyna... more Introduction: This study aimed to evaluate the effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. Methods and Materials: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture, the ro...
ABSTRACTObjective: this questionnaire aimed to evaluate the approval of a new pictograph system f... more ABSTRACTObjective: this questionnaire aimed to evaluate the approval of a new pictograph system for endodontic diagnosis by undergraduate and graduate students/endodontist instead of a conventional method. Methods: after a presentation to a total of 224 participants explaining the pictograph system, the participants (166 undergraduate students and 58 graduate students/endodontist) received a standardized questionnaire with three questions. The data were analyzed using the multprop macro basing on Tukey's test for multiple comparison with (P ? 0.05). The first question was to define the education level of the participants; the second question about which diagnosis method is preferred; and the third question about the intention of using one of the methods in the future. The questionnaire was realized in May 2019. The response rate was 97.7%. Results: the first question showed that 74.10% and 25.90% were undergraduate and graduate students/endodontist respectively. After statistic...
Background: This study aimed to compare the temperature increase produced by halogen bulb (HAL) a... more Background: This study aimed to compare the temperature increase produced by halogen bulb (HAL) and light-emitting diode (LED) light curing units (LCUs) by irradiating dentin discs (0.5 mm and 1 mm thickness), and to evaluate their cytotoxic effects on fibroblast culture in the presence of dentin discs due to the increasing demand on resin composite restorations and teeth bleaching for esthetic purposes. Methods: A total of 20 bovine incisors were used to obtain dentin discs and divided into four experimental groups (n=10): HAL0.5: irradiation with halogen-tungsten bulb Curing Light XL 3000 at an intensity of 470 mW/cm2 over a dentin disc of 0.5 mm; LED0.5: irradiation with LED Optilight Max (GNATUSRibeirão Preto, SP, Brazil) at an intensity of 1200 mW/cm2 over a dentin disc of 0.5 mm; HAL1: irradiation as in HAL0.5 but over a dentin disc of 1 mm; LED1: irradiation as in LED0.5 but over a dentin disc of 1 mm. The temperature increase was measured using a digital thermometer and the ...
Objective: This systematic review aimed to discuss a possible effect of sodium hypochlorite (NaOC... more Objective: This systematic review aimed to discuss a possible effect of sodium hypochlorite (NaOCl) over the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the new generation of corona virus, which was reported firstly in December 2019 in Wuhan, China. Methods: Electronic search was performed for studies up to March 31, 2021 in the following databases: PubMed, LILACS, BBO, Scopus, web of science and Cochrane considering clinical trials and laboratory studies that evaluated the action of NaOCl over SARS-CoV-2. Results: Numerous founded studies reported effectivity of NaOCl in different concentrations and exposure times over corona viruses including the SARS-CoV-2. However, none of the studies evaluated this action in the root canal system. No metanalysis was conducted due to variability of methods or lack of information of included articles. Conclusion: NaOCl has a possible effectivity over SARS-CoV-2 as it dissolves the organic material of virus membrane, but th...
This study evaluated the effect of different surface treatments on the tensile bond strength betw... more This study evaluated the effect of different surface treatments on the tensile bond strength between lithium disilicate glass-ceramics, resin cement, and dentin. Fifty truncated cone-shape glass-ceramics were divided into five groups (n = 10): G1, control: 10% hydrofluoric acid (HF); G2, Nd:YAG laser + silane; G3, Sil + Nd:YAG laser; G4, graphite + Nd:YAG laser + Sil; and G5, graphite + Sil + Nd:YAG laser. Fifty human third-molars were cut to cylindrical shape and polished to standardize the bonding surfaces. The glass-ceramic specimens were bonded to dentin with a dual-cured resin cement and stored in distilled water for 24 h at 37ºC. Tensile testing was performed on a universal testing machine (10 Kgf load cell at 1 mm/min) until failure. The bond strength values (mean ± SD) in MPa were G1 (9.4 ± 2.3), G2 (9.7 ± 2.0), G3 (6.7 ± 1.9), G4 (4.6 ± 1.1), and G5 (1.2 ± 0.3). Nd:YAG laser and HF improve the bond strength between lithium disilicate glass-ceramics, resin cement, and dentin...
Objectives This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic ext... more Objectives This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic extract of Persea americana “P. americana” over multidrug-resistant strains of Acinetobacter baumannii “A. baumannii” as alternative therapy to be investigated. Methods A bacterial inoculum of each bacterial strain (4a, 5a, 9a, 12a, ATCC 19606) of A. baumannii was prepared and adjusted by the spectrophotometer. The microdilution broth method was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). P. americana glycolic extracts were obtained of the tree stalk and leaves. The biofilm viability was tested by MTT assay after 5 min exposure. The toxicity of the extracts was tested by invertebrate model Galleria mellonella. The data were analyzed by ANOVA, Tukey test and log-rank method (α=0.05). Results The extract showed an inhibitory and bactericidal action over all the tested strains with the lowest MIC value observed for the referen...
Objetivo: Este trabalho foi realizado para identificar os maiores fatores responsáveis pelo desen... more Objetivo: Este trabalho foi realizado para identificar os maiores fatores responsáveis pelo desencadeamento de ansiedade nos pacientes atendidos em clínica escola de odontologia. Metodologia: total de 87 participantes (entre 18-70 anos) foram incluidos neste estudo após autorização dos mesmos por meio de um Termo de Consentimento Livre e Esclarecido. Foi utilizado um questionário para avaliar a ansiedade frente ao tratamento odontológico. Este trabalho foi realizado na disciplina de clínica integrada do Instituto de Ensino Superior e Pós-Graduação Padre Gervásio – INAPÓS após aprovação do comitê de ética em pesquisa que envolve seres humanos da Universidade Estadual de Campinas (no 4.989.109). Os dados obtidos foram apresentados em porcentagem. Resultados: Dos 87 voluntários, 28,7% relataram ter medo de dentista, sendo que a agulha (22,4%) e o medo de sentir dor (32,7%) foram os principais fatores apontados como responsáveis pelo desencadeamento da ansiedade durante a consulta odont...
This study aimed to evaluate the efficacy of passive ultrasonic irrigation (PUI) on dissolving th... more This study aimed to evaluate the efficacy of passive ultrasonic irrigation (PUI) on dissolving the organic tissue inside simulated internal root resorption (IRR) using sodium hypochlorite (NaOCl) or chlorhexidine (CHX). A total of 40 human lower premolars were collected based on dimensional and morphological similarities. The roots were embedded in cylinders (3 cm diameter; 2.5 cm height) of self-cured acrylic resin, and then an IRR was simulated. The specimens were divided into 4 groups (n = 10) according to irrigation protocols: group 1: CHX + PUI; group 2: CHX; group 3: NaOCl + PUI; group 4: NaOCl. The total irrigation time was 150 s at a flow rate of 5 mL/min. A tissue mass of porcine palatine mucosa was used to simulate the organic tissue, it was weighed before and after the irrigation using an analytic balance, and the difference between both readings was calculated and transferred to percentage values. Data were submitted to statistical analysis using two-way ANOVA (factors: ...
Aim: The aim was to compare cyclic fatigue, torsional failure, and flexural resistance of NiTi en... more Aim: The aim was to compare cyclic fatigue, torsional failure, and flexural resistance of NiTi endodontic files: Hyflex CM (HYF), Genius files (GEN), WaveOne Gold (WOG), and ProTaper Universal (PTU). Materials and Methods: Fifteen files of each brand were used in cyclic fatigue test and other fifteen files for flexural test and torsional failure test. To the cyclic fatigue test, used torque limit and revolutions per minute were set according to the respective manufacturer guidelines. The test was performed under deionized water at 36°C, and all files were tested in a 3 mm radius of curvature with an angle of curvature of 60°, time of the fracture was recorded. Torsional fatigue test was performed in the torsional machine (Instron MT, USA), recording the fractured time and torque data by the machine software. Flexural fatigue test was performed in 60° of curvature. All data were statistically analyzed by one-way analysis of variance, and Tukey test for multiple comparisons. Results: Cyclic fatigue (seconds)= HYF: 744.1 ± 231.9/GEN: 477.3 ± 220.5/WO: 278.4 ± 57.0/PTU: 152.4 ± 65.2; torsional failure (N × cm)= HYF: 6.85 ± 1.484/GEN: 6.55 ± 0.828/WOG: 5.73 ± 0.360/PTU: 4.43 ± 0.900; flexural resistance (N × mm)= HYF: 0.33 ± 0.294/GEN: 0.19 ± 0.136/WOG: 0.98 ± 0.216/PTU: 1.85 ± 0.276. Conclusion: HYF and GEN showed the best results for cyclic fatigue, torsional failure, and flexural resistance, followed by WOG and PTU.
Endodontic perforations are common accidents that occasionally happen as a result of misuse or di... more Endodontic perforations are common accidents that occasionally happen as a result of misuse or difficult anatomy of some teeth; it may lead to teeth loss unless a good management is provided. Bioceramic (silicate-based) cements like mineral trioxide aggregate have a big role in management of such accidents. This case report aimed to evaluate the ability of five mineral oxides cement “5MO” in sealing two root canal perforations (furcation and postdrill perforations) and inducing clinical and radiographic healing in the periodontal tissues with/without the use of collagen sponge matrix. A 58-year-old healthy female was referred to our dental office complaining of severe pain in the upper left premolars’ region. Periapical radiographic examination revealed unsatisfactory root canal treatment of the teeth #24 and #25 with a furcation perforation and a postdrill perforation, respectively. Cone-beam computed tomography “CBCT” scans confirmed the findings of the periapical radiography and ...
Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The pu... more Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The purpose of this study was to evaluate the effect of NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on Enterococcus faecalis, Escherichia coli, and their endotoxins, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Methods: 40 human lower premolars were contaminated with E. coli (ATCC 25922) for 28 days and E. faecalis (ATCC 29212) for 21 days. Specimens were randomly divided into four groups: (1) 2.5% NaOCl irrigating the canals without PUI activation; (2) 2.5% NaOCl with PUI; (3) 3% NaOCl gel irrigating the canals without PUI; and (4) 3% NaOCl gel with PUI. 40 mL of irrigant was used for each group. PUI activation was carried out using E1-Irrisonic stainless-steel tip at 10% frequency. After treatment, all specimens were filled with 3mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3min and then washed with nonpyrogenic saline solution. Three ...
Root perforation is a common endodontic accident. Its management depends mainly on root canal dis... more Root perforation is a common endodontic accident. Its management depends mainly on root canal disinfection and sealing the perforation area by preventing any communication with the periodontium to prevent recontamination. A patient was referred to treat root perforation due to a previous treatment of tooth #22. The diagnosis was symptomatic periapical periodontitis, and the treatment plan was to retreat the root canal of #22 and make a surgical intervention (apicoectomy) associated with antimicrobial photodynamic therapy as a complementary technique. Five mineral oxides (5MO) cement was used as a root-end filling material. The procedures were performed in two sessions and controlled in two visits (after 30 days and 12 months). A bone neoformation was observed at the periapical area of tooth #22. 5MO bioceramic cement was effective in inducing the repair of the periapical lesion and had the ability to seal the exposed periapical area of the tooth. Its success depended mainly on root ...
Objective: Treponema denticola “T. denticola” is a pathogen associated with periodontal diseases ... more Objective: Treponema denticola “T. denticola” is a pathogen associated with periodontal diseases that exhibits capacity for adherence, invasion, and colonization of host tissues, which allows alternating its location and damage in different sites of human body. This review aimed to discuss different studies that detected T. denticola in atherosclerotic plaques, demonstrating the importance of periodontal disease on the systemic health and the necessity of exploring the outcome of this colonization apart from the oral cavity. Methodology: Fifty-five studies were identified and gathered in this review according to the following topics: Periodontal disease, atherosclerosis and T. denticola. In vitro and in vivo studies published between 2002 and 2020 were searched on PubMed, raising relevant insights about the role of T. denticola and its association with the systemic disease, atherosclerosis, focusing on the bacterial tissue invasion and development of atherosclerosis. Results: After ...
Oral Surgery, Oral Medicine, Oral Pathology, and Oral Radiology, 2020
This report describes the clinical and surgical approach of a complex odontoma with periapical in... more This report describes the clinical and surgical approach of a complex odontoma with periapical involvement implementing a new bioceramic cement for retrobturation. The patient presented for routine consultation with slight dental mobility in the anterosuperior region. On clinical, radiographic and computed tomography examinations, bone rarefaction was observed in the apical region of the elements 11 and 12, with density higher than bone and similar to teeth, 2.5 cm in diameter, external root resorption of the elements involved, and pulp necrosis of the 12. Conventional endodontic treatment was performed. In a second intervention, a surgical excision of the lesion was performed, showing a mass with clinical characteristics of complex odontoma. Element 12 was retrobturated with Aggregate Pentaoxide Mineral Bioceramic Cement (5 MO). Histopathological examination confirmed the diagnosis of complex odontoma. On follow-up there were no reports of symptoms and the clinical signs were satis...
Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South A... more Objective: Achyrocline satureioides “A.satureioides” is a subshrub, widely distributed in South America because of its medicinal properties. Although it is widely used in folk medicine, there is still no approval for its therapeutic use, and its biocompatibility is little explored. This study aimed to evaluate its cytotoxicity and genotoxicity over human gingival fibroblasts (FMM-1). Methodology: Ten different concentration of the glycolic extract of A.satureioides were tested for 5min and 24h of contact with the cells to evaluate its cytotoxicity using the MTT colorimetric assay and to evaluate its genotoxicity using the micronucleus assay. Data were analyzed with one-way ANOVA and Tukey test with a significance level (α=0.05). Results: All tested concentrations of the extract presented cell viability more than 70% and has no significant difference of the control group after 5min and 24h. However, for 5min the 100 mg/mL was cytotoxic and for 24h the 1.56 mg/mL stimulated cell prol...
Objective: Covid-19 is a highly infectious disease; it is transmitted by patients’ secretions and... more Objective: Covid-19 is a highly infectious disease; it is transmitted by patients’ secretions and respiratory droplets SARS-CoV-2 was detected in patients saliva making dental care professionals more susceptible for contamination and the patient less safe while seeking the dental treatment. Methodology: This quantitative study was elaborated through a questionnaire for patients who frequently seek dental care in private and publica dental care units. A total of 205 over-18 patients were included, those who left one or more questions without answer were excluded. Participants were able to refuse to answer any question or to participate in the questionnaire at all. The questionnaire was composed of five questions. Participants were divided into two groups: Group 1: private dental offices patients (n=98); Group 2: public dental clinic patients (n=107). Results: there was a significant difference between groups 1 and 2, in which 50% of group 1 feel safe while seeking dental care during ...
Introduction: This study aimed to evaluate the effectiveness of N-acetylcysteine (NAC), photodyna... more Introduction: This study aimed to evaluate the effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. Methods and Materials: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture, the ro...
ABSTRACTObjective: this questionnaire aimed to evaluate the approval of a new pictograph system f... more ABSTRACTObjective: this questionnaire aimed to evaluate the approval of a new pictograph system for endodontic diagnosis by undergraduate and graduate students/endodontist instead of a conventional method. Methods: after a presentation to a total of 224 participants explaining the pictograph system, the participants (166 undergraduate students and 58 graduate students/endodontist) received a standardized questionnaire with three questions. The data were analyzed using the multprop macro basing on Tukey's test for multiple comparison with (P ? 0.05). The first question was to define the education level of the participants; the second question about which diagnosis method is preferred; and the third question about the intention of using one of the methods in the future. The questionnaire was realized in May 2019. The response rate was 97.7%. Results: the first question showed that 74.10% and 25.90% were undergraduate and graduate students/endodontist respectively. After statistic...
Background: This study aimed to compare the temperature increase produced by halogen bulb (HAL) a... more Background: This study aimed to compare the temperature increase produced by halogen bulb (HAL) and light-emitting diode (LED) light curing units (LCUs) by irradiating dentin discs (0.5 mm and 1 mm thickness), and to evaluate their cytotoxic effects on fibroblast culture in the presence of dentin discs due to the increasing demand on resin composite restorations and teeth bleaching for esthetic purposes. Methods: A total of 20 bovine incisors were used to obtain dentin discs and divided into four experimental groups (n=10): HAL0.5: irradiation with halogen-tungsten bulb Curing Light XL 3000 at an intensity of 470 mW/cm2 over a dentin disc of 0.5 mm; LED0.5: irradiation with LED Optilight Max (GNATUSRibeirão Preto, SP, Brazil) at an intensity of 1200 mW/cm2 over a dentin disc of 0.5 mm; HAL1: irradiation as in HAL0.5 but over a dentin disc of 1 mm; LED1: irradiation as in LED0.5 but over a dentin disc of 1 mm. The temperature increase was measured using a digital thermometer and the ...
Objective: This systematic review aimed to discuss a possible effect of sodium hypochlorite (NaOC... more Objective: This systematic review aimed to discuss a possible effect of sodium hypochlorite (NaOCl) over the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the new generation of corona virus, which was reported firstly in December 2019 in Wuhan, China. Methods: Electronic search was performed for studies up to March 31, 2021 in the following databases: PubMed, LILACS, BBO, Scopus, web of science and Cochrane considering clinical trials and laboratory studies that evaluated the action of NaOCl over SARS-CoV-2. Results: Numerous founded studies reported effectivity of NaOCl in different concentrations and exposure times over corona viruses including the SARS-CoV-2. However, none of the studies evaluated this action in the root canal system. No metanalysis was conducted due to variability of methods or lack of information of included articles. Conclusion: NaOCl has a possible effectivity over SARS-CoV-2 as it dissolves the organic material of virus membrane, but th...
This study evaluated the effect of different surface treatments on the tensile bond strength betw... more This study evaluated the effect of different surface treatments on the tensile bond strength between lithium disilicate glass-ceramics, resin cement, and dentin. Fifty truncated cone-shape glass-ceramics were divided into five groups (n = 10): G1, control: 10% hydrofluoric acid (HF); G2, Nd:YAG laser + silane; G3, Sil + Nd:YAG laser; G4, graphite + Nd:YAG laser + Sil; and G5, graphite + Sil + Nd:YAG laser. Fifty human third-molars were cut to cylindrical shape and polished to standardize the bonding surfaces. The glass-ceramic specimens were bonded to dentin with a dual-cured resin cement and stored in distilled water for 24 h at 37ºC. Tensile testing was performed on a universal testing machine (10 Kgf load cell at 1 mm/min) until failure. The bond strength values (mean ± SD) in MPa were G1 (9.4 ± 2.3), G2 (9.7 ± 2.0), G3 (6.7 ± 1.9), G4 (4.6 ± 1.1), and G5 (1.2 ± 0.3). Nd:YAG laser and HF improve the bond strength between lithium disilicate glass-ceramics, resin cement, and dentin...
Objectives This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic ext... more Objectives This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic extract of Persea americana “P. americana” over multidrug-resistant strains of Acinetobacter baumannii “A. baumannii” as alternative therapy to be investigated. Methods A bacterial inoculum of each bacterial strain (4a, 5a, 9a, 12a, ATCC 19606) of A. baumannii was prepared and adjusted by the spectrophotometer. The microdilution broth method was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). P. americana glycolic extracts were obtained of the tree stalk and leaves. The biofilm viability was tested by MTT assay after 5 min exposure. The toxicity of the extracts was tested by invertebrate model Galleria mellonella. The data were analyzed by ANOVA, Tukey test and log-rank method (α=0.05). Results The extract showed an inhibitory and bactericidal action over all the tested strains with the lowest MIC value observed for the referen...
Objetivo: Este trabalho foi realizado para identificar os maiores fatores responsáveis pelo desen... more Objetivo: Este trabalho foi realizado para identificar os maiores fatores responsáveis pelo desencadeamento de ansiedade nos pacientes atendidos em clínica escola de odontologia. Metodologia: total de 87 participantes (entre 18-70 anos) foram incluidos neste estudo após autorização dos mesmos por meio de um Termo de Consentimento Livre e Esclarecido. Foi utilizado um questionário para avaliar a ansiedade frente ao tratamento odontológico. Este trabalho foi realizado na disciplina de clínica integrada do Instituto de Ensino Superior e Pós-Graduação Padre Gervásio – INAPÓS após aprovação do comitê de ética em pesquisa que envolve seres humanos da Universidade Estadual de Campinas (no 4.989.109). Os dados obtidos foram apresentados em porcentagem. Resultados: Dos 87 voluntários, 28,7% relataram ter medo de dentista, sendo que a agulha (22,4%) e o medo de sentir dor (32,7%) foram os principais fatores apontados como responsáveis pelo desencadeamento da ansiedade durante a consulta odont...
This study aimed to evaluate the efficacy of passive ultrasonic irrigation (PUI) on dissolving th... more This study aimed to evaluate the efficacy of passive ultrasonic irrigation (PUI) on dissolving the organic tissue inside simulated internal root resorption (IRR) using sodium hypochlorite (NaOCl) or chlorhexidine (CHX). A total of 40 human lower premolars were collected based on dimensional and morphological similarities. The roots were embedded in cylinders (3 cm diameter; 2.5 cm height) of self-cured acrylic resin, and then an IRR was simulated. The specimens were divided into 4 groups (n = 10) according to irrigation protocols: group 1: CHX + PUI; group 2: CHX; group 3: NaOCl + PUI; group 4: NaOCl. The total irrigation time was 150 s at a flow rate of 5 mL/min. A tissue mass of porcine palatine mucosa was used to simulate the organic tissue, it was weighed before and after the irrigation using an analytic balance, and the difference between both readings was calculated and transferred to percentage values. Data were submitted to statistical analysis using two-way ANOVA (factors: ...
Aim: The aim was to compare cyclic fatigue, torsional failure, and flexural resistance of NiTi en... more Aim: The aim was to compare cyclic fatigue, torsional failure, and flexural resistance of NiTi endodontic files: Hyflex CM (HYF), Genius files (GEN), WaveOne Gold (WOG), and ProTaper Universal (PTU). Materials and Methods: Fifteen files of each brand were used in cyclic fatigue test and other fifteen files for flexural test and torsional failure test. To the cyclic fatigue test, used torque limit and revolutions per minute were set according to the respective manufacturer guidelines. The test was performed under deionized water at 36°C, and all files were tested in a 3 mm radius of curvature with an angle of curvature of 60°, time of the fracture was recorded. Torsional fatigue test was performed in the torsional machine (Instron MT, USA), recording the fractured time and torque data by the machine software. Flexural fatigue test was performed in 60° of curvature. All data were statistically analyzed by one-way analysis of variance, and Tukey test for multiple comparisons. Results: Cyclic fatigue (seconds)= HYF: 744.1 ± 231.9/GEN: 477.3 ± 220.5/WO: 278.4 ± 57.0/PTU: 152.4 ± 65.2; torsional failure (N × cm)= HYF: 6.85 ± 1.484/GEN: 6.55 ± 0.828/WOG: 5.73 ± 0.360/PTU: 4.43 ± 0.900; flexural resistance (N × mm)= HYF: 0.33 ± 0.294/GEN: 0.19 ± 0.136/WOG: 0.98 ± 0.216/PTU: 1.85 ± 0.276. Conclusion: HYF and GEN showed the best results for cyclic fatigue, torsional failure, and flexural resistance, followed by WOG and PTU.
Endodontic perforations are common accidents that occasionally happen as a result of misuse or di... more Endodontic perforations are common accidents that occasionally happen as a result of misuse or difficult anatomy of some teeth; it may lead to teeth loss unless a good management is provided. Bioceramic (silicate-based) cements like mineral trioxide aggregate have a big role in management of such accidents. This case report aimed to evaluate the ability of five mineral oxides cement “5MO” in sealing two root canal perforations (furcation and postdrill perforations) and inducing clinical and radiographic healing in the periodontal tissues with/without the use of collagen sponge matrix. A 58-year-old healthy female was referred to our dental office complaining of severe pain in the upper left premolars’ region. Periapical radiographic examination revealed unsatisfactory root canal treatment of the teeth #24 and #25 with a furcation perforation and a postdrill perforation, respectively. Cone-beam computed tomography “CBCT” scans confirmed the findings of the periapical radiography and ...
Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The pu... more Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The purpose of this study was to evaluate the effect of NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on Enterococcus faecalis, Escherichia coli, and their endotoxins, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Methods: 40 human lower premolars were contaminated with E. coli (ATCC 25922) for 28 days and E. faecalis (ATCC 29212) for 21 days. Specimens were randomly divided into four groups: (1) 2.5% NaOCl irrigating the canals without PUI activation; (2) 2.5% NaOCl with PUI; (3) 3% NaOCl gel irrigating the canals without PUI; and (4) 3% NaOCl gel with PUI. 40 mL of irrigant was used for each group. PUI activation was carried out using E1-Irrisonic stainless-steel tip at 10% frequency. After treatment, all specimens were filled with 3mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3min and then washed with nonpyrogenic saline solution. Three ...
Root perforation is a common endodontic accident. Its management depends mainly on root canal dis... more Root perforation is a common endodontic accident. Its management depends mainly on root canal disinfection and sealing the perforation area by preventing any communication with the periodontium to prevent recontamination. A patient was referred to treat root perforation due to a previous treatment of tooth #22. The diagnosis was symptomatic periapical periodontitis, and the treatment plan was to retreat the root canal of #22 and make a surgical intervention (apicoectomy) associated with antimicrobial photodynamic therapy as a complementary technique. Five mineral oxides (5MO) cement was used as a root-end filling material. The procedures were performed in two sessions and controlled in two visits (after 30 days and 12 months). A bone neoformation was observed at the periapical area of tooth #22. 5MO bioceramic cement was effective in inducing the repair of the periapical lesion and had the ability to seal the exposed periapical area of the tooth. Its success depended mainly on root ...
Objective: Treponema denticola “T. denticola” is a pathogen associated with periodontal diseases ... more Objective: Treponema denticola “T. denticola” is a pathogen associated with periodontal diseases that exhibits capacity for adherence, invasion, and colonization of host tissues, which allows alternating its location and damage in different sites of human body. This review aimed to discuss different studies that detected T. denticola in atherosclerotic plaques, demonstrating the importance of periodontal disease on the systemic health and the necessity of exploring the outcome of this colonization apart from the oral cavity. Methodology: Fifty-five studies were identified and gathered in this review according to the following topics: Periodontal disease, atherosclerosis and T. denticola. In vitro and in vivo studies published between 2002 and 2020 were searched on PubMed, raising relevant insights about the role of T. denticola and its association with the systemic disease, atherosclerosis, focusing on the bacterial tissue invasion and development of atherosclerosis. Results: After ...
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