This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, dige... more This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, digesta pH, ileal populations of Clostridium perfringens and lactic acid bacteria, ileal nutrient digestibility, digestive enzyme activity, and mRNA expression of intestinal nutrient transporters in starter broilers challenged with C. perfringens. The experiment was conducted in a 2×2 factorial arrangement with two levels of CA (0 and 30 g/kg) and 2 levels of xylanase (0 and 200 mg/kg). Each of the four dietary treatments was fed to six replicate pens (15 birds/pen) between 0 and 21 d of age. Dietary CA significantly increased ADFI and ADG; meanwhile, xylanase addition led to a substantial reduction in FCR (P＜0.05). No differences in digesta pH, C. perfringens counts, or quantity of lactic acid bacteria were found between the treatments. Xylanase supplementation increased AME values (P＜0.01) and ileal digestibility of CP (P＜0.05) in challenged birds. The inclusion of CA also increased the AME (P＜0.01), and tended to increased ileal CP digestibility (P＝0.085). Xylanase supplementation increased α-amylase, trypsin, and sucrose activity in the jejunum (P＜0.01). Dietary CA significantly increased (P＜0.01) villi length as well as the villus length to crypt depth ratio in jejunum segments. The jejunal mRNA expression of sodium glucose co-transporter 1 (SGLT1) and H +-dependent peptide transporter 1 (PepT1) were upregulated by xylanase supplementation (P＜0.01). The results suggest that dietary CA can promote growth as well as improve intestinal morphology and AME in birds challenged with necrotic enteritis. This study shows that xylanase supplementation improved FCR and AME in birds independent of C. perfringens infection; it also elevated the apparent ileal digestibility of CP, digestive enzyme activities, and mRNA expression of nutrient transporters in challenged birds.
In current experiment, a total of 180 day-old unsexed Ross 308 broiler chicks were randomly alloc... more In current experiment, a total of 180 day-old unsexed Ross 308 broiler chicks were randomly allocated into three treatments and four replications of fifteen chicks each, in a completely randomized experimental design manner. Diets prepared without additive as Control (group1); 0.1% probiotic (group2) and 0.1% AGP (group 3). Results showed that addition of AGP and probiotic could significantly decrease the population of coliforms, salmonella and E.coli in broilers intestine and villus height (µm) and Villus height to crypt depth ratio was increased in treatment having probiotic containing Saccharomyces cerevisiae, whereas, the number of goblet cells in each 100 µm of villus height and also crypt depth ratio was significantly decreased in probiotic and AGP fed groups. It can be concluded that dietary probiotic could be positive replacer to antibiotic growth promoters, when included in to the basal diet of the current experiment.
Aflatoxins - Biochemistry and Molecular Biology, 2011
Page 1. 11 Aflatoxicosis in Layer and Breeder Hens Milad Manafi Department of Animal Science, Mal... more Page 1. 11 Aflatoxicosis in Layer and Breeder Hens Milad Manafi Department of Animal Science, Malayer University, Malayer Iran 1. Introduction During the past few decades there has been a steady increase in global production of poultry meat and eggs. ...
This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, dige... more This study was conducted to evaluate the effects of xylanase and citric acid (CA) on growth, digesta pH, ileal populations of Clostridium perfringens and lactic acid bacteria, ileal nutrient digestibility, digestive enzyme activity, and mRNA expression of intestinal nutrient transporters in starter broilers challenged with C. perfringens. The experiment was conducted in a 2×2 factorial arrangement with two levels of CA (0 and 30 g/kg) and 2 levels of xylanase (0 and 200 mg/kg). Each of the four dietary treatments was fed to six replicate pens (15 birds/pen) between 0 and 21 d of age. Dietary CA significantly increased ADFI and ADG; meanwhile, xylanase addition led to a substantial reduction in FCR (P＜0.05). No differences in digesta pH, C. perfringens counts, or quantity of lactic acid bacteria were found between the treatments. Xylanase supplementation increased AME values (P＜0.01) and ileal digestibility of CP (P＜0.05) in challenged birds. The inclusion of CA also increased the AME (P＜0.01), and tended to increased ileal CP digestibility (P＝0.085). Xylanase supplementation increased α-amylase, trypsin, and sucrose activity in the jejunum (P＜0.01). Dietary CA significantly increased (P＜0.01) villi length as well as the villus length to crypt depth ratio in jejunum segments. The jejunal mRNA expression of sodium glucose co-transporter 1 (SGLT1) and H +-dependent peptide transporter 1 (PepT1) were upregulated by xylanase supplementation (P＜0.01). The results suggest that dietary CA can promote growth as well as improve intestinal morphology and AME in birds challenged with necrotic enteritis. This study shows that xylanase supplementation improved FCR and AME in birds independent of C. perfringens infection; it also elevated the apparent ileal digestibility of CP, digestive enzyme activities, and mRNA expression of nutrient transporters in challenged birds.
In current experiment, a total of 180 day-old unsexed Ross 308 broiler chicks were randomly alloc... more In current experiment, a total of 180 day-old unsexed Ross 308 broiler chicks were randomly allocated into three treatments and four replications of fifteen chicks each, in a completely randomized experimental design manner. Diets prepared without additive as Control (group1); 0.1% probiotic (group2) and 0.1% AGP (group 3). Results showed that addition of AGP and probiotic could significantly decrease the population of coliforms, salmonella and E.coli in broilers intestine and villus height (µm) and Villus height to crypt depth ratio was increased in treatment having probiotic containing Saccharomyces cerevisiae, whereas, the number of goblet cells in each 100 µm of villus height and also crypt depth ratio was significantly decreased in probiotic and AGP fed groups. It can be concluded that dietary probiotic could be positive replacer to antibiotic growth promoters, when included in to the basal diet of the current experiment.
Aflatoxins - Biochemistry and Molecular Biology, 2011
Page 1. 11 Aflatoxicosis in Layer and Breeder Hens Milad Manafi Department of Animal Science, Mal... more Page 1. 11 Aflatoxicosis in Layer and Breeder Hens Milad Manafi Department of Animal Science, Malayer University, Malayer Iran 1. Introduction During the past few decades there has been a steady increase in global production of poultry meat and eggs. ...
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