Sukriye Celikkol

Bu çalışmada, kâğıt endüstrisi atıksularını arıtan gerçek ölçekli bir anaerobik kontak reaktörün 3 farklı yüksekliğinden 2 farklı zamanda alınan çamur numunelerinin mikrobiyal komünite yapıları Denatüran Gradyan Jel Elektroforez (DGGE) yöntemi kullanılarak karşılaştırılmıştır. 2 aylık izleme dönemi içinde sistem 2 hafta süreyle bakıma alınmıştır. Kontak reaktörün 1.6-1.8 kg KOİ/m3.gün organik yükleme hızında, KOİ giderim verimi % 47-55, metan üretim verimi 0.18-0.20 m3CH4/kgKOİgiderilen aralığında değişmiştir. DGGE analizleri sonucu, arkeyal popülasyona ait 31, bakteriyel popülasyona ait 57 farklı tür tespit edilmiştir. Arkeyal popülasyona ait 3 farklı tür Ağustos 2005'te tespit edilememiş, buna karşın 6 yeni tür gözlenmiştir. Bakteriyel popülasyonda ise Temmuz 2005 numunesine ait 10 farklı tür Ağustos 2005 numunesinde t...

Weathering of two church facades in Rio de Janeiro was caused substantially by salts, mainly halite and gypsum, detected by SEM and chemical analyses, which cause physical stresses by deposition within the rock. Biofilm populations, determined by SEM and as operational taxonomic units (OTUs), degraded stone by penetration, solubilization and redeposition of minerals on their surfaces. Endolithic cyanobacteria were associated with gypsum deposits. Microbiomes were typical for high-stress environments, high salt, intense insolation, low water and low nutrients (eg halophilic Rubrobacter, Salinicola, Sterigmatomyces). The main colonizers on the church most affected by traffic (Nossa Senhora da Candelária - CA) were Actinobacteria; Gammaproteobacteria (chiefly Pseudomonas) were predominant on the site situated in a leafy square (São Francisco de Paula - SF). Major Gammaproteobacteria on CA were halophilic Halomonas and Rhodobacteriaceae. Fungal OTUs on both churches were principally dim...

Bacteria, algae and fungi colonising dry (F1) and wet (F2) white painted walls in the fortress of Santa Cruz da Barra, Niteroi, Brazil, were detected using field emission-scanning electron microscopy (FE-SEM) and next generation DNA sequencing (NGS) techniques. Major bacterial phyla Operational Taxonomic Units (OTUs) detected were Chloroflexi in the wet green biofilm F2 (38.85% compared with 7.56% in F1) and Proteobacteria in the dry grey biofilm F1 (57.17% compared with 28.69% in F2). Diatoms were detected at both sites by FE-SEM, but only at F1 by NGS. More algae and cyanobacteria were identified at F1, and this was the only biofilm containing Archaea, possibly related to the high level of salt efflorescences at this site. Although thinner, F1 biofilm showed considerably higher genus richness than the wet biofilm, F2. The thickness and appearance of the biofilms did not correlate with their genomic complexity. Ascomycetes of the Sordariomycetes were major fungi identified at both sites, Khuskia (3.33% OTUs) at F1 and Emericellopsis (7.99% OTUs) at F2, and few filamentous forms were seen by microscopy. However, many fungal OTUs could not be identified to phylum level. Potential bacterial and fungal paint deteriogens were detected at both sites. The results confirmed the importance of using a variety of techniques in the study of microbial communities.

16S rRNA gene profiling using a pipeline involving the Greengenes database revealed that bacterial populations in innermost (proximal to the steel surface) and outer regions of biofilms on carbon steel exposed 3 m below the surface at an offshore site in the Gulf of Guinea differed from one another and from seawater. There was a preponderance of gammaproteobacterial sequences, representing organisms known for hydrocarbon degradation. Total DNA from the innermost layer was 1500 times that recovered from the outermost. Stramenopiles (diatom) sequences were prevalent in the former. Rhodobacteriaceae, key biofilm formers, comprised 14.9% and 4.22% OTUs of inner and outer layers, respectively. Photosynthetic anaerobic sulfur oxidizer sequences were also prominent in the biofilms. Analysis of data using a different pipeline with Silva111 allowed detection of 0.3-0.4% SRB in the biofilms. The high abundance of aerobic micro-algal sequences in inner biofilm suggests they are initial coloniz...

Industrial wastewaters have turned out to be a significant problem during the last decades. The best alternative for maize processing wastewaters due to high organic content and slow biodegradability characteristics is anaerobic treatment, which is successfully used in the treatment of medium-high strength industrial wastewaters. This study investigates a full-scale anaerobic expanded granular sludge bed reactor treating a maize industry wastewater in terms of reactor operation, methanogenic activity of reactor sludge, changes in composition and numbers of microbial populations during a 5-month period. Three samples were taken in 2-months intervals when the reactor was re-operated after a shut-down of 2 months. Combination of denaturing gradient gel electrophoresis (DGGE), quantitative real time polymerase chain reactions (Q-PCR) and specific methanogenic activity tests were used to investigate composition, diversity and quantity of microbial community with activity of acetoclastic methanogens. During monitoring period, COD removal efficiencies and organic loading rates varied in ranges of 79–95% and 0.65–3.85 kg COD/m3.d, respectively. Potential methane production rates of the reactor sludges decreased 27% with time that is from 394 to 287 mL CH4/gVSS.d. Archaea, bacteria, and methanogens in the sludge samples were quantified by Q-PCR and the results showed that while amount of archaea was decreasing, quantity of methanogens increased during the monitoring time. The DGGE results of the anaerobic sludge revealed that the microbial diversity dramatically changed, particularly in the last sample. Among these, the differences in archaeal community were more remarkable. Although an average COD removal efficiency of 86%±8.2 which was quite a reasonably stable performance was maintained during the monitoring period, remarkable differences were observed on both acetoclastic methanogenic activity and the methanogenic community. The increase in the numbers of total methanogens despite the decrease of acetoclastic methanogenic activity might be an indicator of dominating hydrogenotrophic methanogenic pathway. Further, construction of clone library was necessary to identify the species and their changes within the all three samples.

Anthropogenic activities release large amounts of nitrogen (N) and phosphorus (P) nutrients into the environment. Sources of nutrients include surface and sub-surface runoffs from agricultural practices with the application of chemical fertilizers and manure as well as combined sewer overflows (CSOs). Nutrient runoffs contribute to the eutrophication of aquatic ecosystems and enhance the growth of cyanobacteria. Precipitation is an important driving force behind the runoff of nutrients from agricultural fields into surrounding water bodies. To understand the dynamics between nutrient input, precipitation and cyanobacterial growth in Missisquoi Bay, Lake Champlain (Quebec), one location in Pike River (a major tributary into the bay) and four locations in Missisquoi Bay were monitored from April to November in 2017 and 2018. Biweekly water samples were analyzed using chemical methods and high-throughput sequencing of 16S rRNA gene amplicons. High concentrations of N and P were typical...