ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining o... more ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining over the decades and it has an adverse impact on human health and food security. Hence, soil health restoration is a need of the hour. It is known that microorganisms play a vital role in remediation of soil pollutants like heavy metals, pesticides, hydrocarbons, etc. However, the indigenous microbes have a limited capacity to degrade these pollutants and it will be a slow process. Genetically modified organisms (GMOs) can catalyze the degradation process as their altered metabolic pathways lead to hypersecretions of various biomolecules that favor the bioremediation process. This review provides an overview on the application of bioengineered microorganisms for the restoration of soil health by degradation of various pollutants. It also sheds light on the challenges of using GMOs in environmental application as their introduction may affect the normal microbial community in soil. Since soil health also refers to the potential of native organisms to survive, the possible changes in the native microbial community with the introduction of GMOs are also discussed. Finally, the future prospects of using bioengineered microorganisms in environmental engineering applications to make the soil fertile and healthy have been deciphered. With the alarming rates of soil health loss, the treatment of soil and soil health restoration need to be fastened to a greater pace and the combinatorial efforts unifying GMOs, plant growth-promoting rhizobacteria, and other soil amendments will provide an effective solution to soil heath restoration ten years ahead. Graphical Abstract Schematic Representation Of Approaches Used To Restore Soil Health
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2021
Traditionally used herbs have been recognized as an important niche for fungal endophytes and the... more Traditionally used herbs have been recognized as an important niche for fungal endophytes and the interaction between them has prompted the invention of novel metabolite. In this regard, the presen...
ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining o... more ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining over the decades and it has an adverse impact on human health and food security. Hence, soil health restoration is a need of the hour. It is known that microorganisms play a vital role in remediation of soil pollutants like heavy metals, pesticides, hydrocarbons, etc. However, the indigenous microbes have a limited capacity to degrade these pollutants and it will be a slow process. Genetically modified organisms (GMOs) can catalyze the degradation process as their altered metabolic pathways lead to hypersecretions of various biomolecules that favor the bioremediation process. This review provides an overview on the application of bioengineered microorganisms for the restoration of soil health by degradation of various pollutants. It also sheds light on the challenges of using GMOs in environmental application as their introduction may affect the normal microbial community in soil. Since soil health also refers to the potential of native organisms to survive, the possible changes in the native microbial community with the introduction of GMOs are also discussed. Finally, the future prospects of using bioengineered microorganisms in environmental engineering applications to make the soil fertile and healthy have been deciphered. With the alarming rates of soil health loss, the treatment of soil and soil health restoration need to be fastened to a greater pace and the combinatorial efforts unifying GMOs, plant growth-promoting rhizobacteria, and other soil amendments will provide an effective solution to soil heath restoration ten years ahead. Graphical Abstract Schematic Representation Of Approaches Used To Restore Soil Health
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2021
Traditionally used herbs have been recognized as an important niche for fungal endophytes and the... more Traditionally used herbs have been recognized as an important niche for fungal endophytes and the interaction between them has prompted the invention of novel metabolite. In this regard, the presen...
International Journal of Tropical Insect Science, 2020
Plant extracts prompting free radical formation causing DNA damage among especially symbiotic mic... more Plant extracts prompting free radical formation causing DNA damage among especially symbiotic microorganisms of Aedes aegypti has not been extensively examined in previous studies. Here, investigated whether: (1) it was possible to reduce the vectorial capacity by eliminating the microbial consortia of Aedes aegypti larvae, (2) the excess formation of free radical could induce damages of genomic DNA and alter the morphological appearance of Aedes aegypti larvae, and (3) which are the probable mechanisms driving the death of mosquitoes that has been treated with phytochemicals. The toxicity of Catunaregam spinosa seed extracts on the microbial community of Aedes aegypti larvae was determined as previously described. The formation of free radicals was confirmed using the ammonium molybdate method. While the GCMS method was used to assess the phytochemical analysis and the DNA lysing potential. The petroleum ether, ethanol, acetone and water extracts of Catunaregam spinosa has exhibited potent toxicity towards Aedes aegypti larvea’s gut bacterial microbiota, including Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis with strong MIC values ranging from 0.07 mg/ml to 5.9 mg/ml. Plant extract induced free radical formations and oxidative damage to DNA. The presence of the bioactive element squalene justifies the insecticidal proprieties of Catunaregam spinosa extract. This study reflects the probable mechanisms underlying the lethal effect of Catunaregam spinosa extract on Aedes aegypti, and it potential as a novel biological vector control approach.
The non-conventional yeasts Kluyveromyces lactis, Yarrowia lipolytica, Ogataea polymorpha and Pic... more The non-conventional yeasts Kluyveromyces lactis, Yarrowia lipolytica, Ogataea polymorpha and Pichia pastoris have been developed as eukaryotic expression hosts because of their desirable growth characteristics, including inhibitor and thermo-tolerance, utilization of diverse carbon substrates, and high amount of extracellular protein secretion. These yeasts already have established in the heterologous production of vaccines, therapeutic proteins, food additives, and bio-renewable chemicals, but recent advances in genetic tool box have the potential to greatly expand and diversify their impact on biotechnology. The diversity of these yeasts includes many strains possessing highly useful, and in some cases even uncommon, metabolic capabilities potentially helpful for bioprocess industry. This review outlines the recent updates of non-conventional yeast in sustainable bioprocesses.
Anionic surfactants like sodium dodecyl sulphate (SDS), due to its extensive disposal to water bo... more Anionic surfactants like sodium dodecyl sulphate (SDS), due to its extensive disposal to water bodies cause detrimental effects to the ecosystem. Among the various attempts to reduce the after effects of these toxicants, microbial induced bioremediation serves as a promising strategy. The current study aimed to develop a three stage bioreactor to remediate anionic surfactants in wastewater using effective bacterial isolates. Screening of effective SDS biodegraders led to isolation of Pseudomonas aeruginosa (MTCC 10311). Treatment of synthetic effluent with an immobilized packed bed reactor at a flow rate of 5 mL h−1 resulted in 81 ± 2% SDS eliminations and 70 ± 1% reduction in chemical oxygen demand (COD) in five cycles (6 h per cycle). The hydraulic retention time of the reactor was found to be 6 h. Combinatorial usage of a three stage bioreactor, involving aeration, adsorption with low cost scrap rubber granules and treatment with immobilized Pseudomonas aeruginosa, successfully r...
ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining o... more ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining over the decades and it has an adverse impact on human health and food security. Hence, soil health restoration is a need of the hour. It is known that microorganisms play a vital role in remediation of soil pollutants like heavy metals, pesticides, hydrocarbons, etc. However, the indigenous microbes have a limited capacity to degrade these pollutants and it will be a slow process. Genetically modified organisms (GMOs) can catalyze the degradation process as their altered metabolic pathways lead to hypersecretions of various biomolecules that favor the bioremediation process. This review provides an overview on the application of bioengineered microorganisms for the restoration of soil health by degradation of various pollutants. It also sheds light on the challenges of using GMOs in environmental application as their introduction may affect the normal microbial community in soil. Since soil health also refers to the potential of native organisms to survive, the possible changes in the native microbial community with the introduction of GMOs are also discussed. Finally, the future prospects of using bioengineered microorganisms in environmental engineering applications to make the soil fertile and healthy have been deciphered. With the alarming rates of soil health loss, the treatment of soil and soil health restoration need to be fastened to a greater pace and the combinatorial efforts unifying GMOs, plant growth-promoting rhizobacteria, and other soil amendments will provide an effective solution to soil heath restoration ten years ahead. Graphical Abstract Schematic Representation Of Approaches Used To Restore Soil Health
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2021
Traditionally used herbs have been recognized as an important niche for fungal endophytes and the... more Traditionally used herbs have been recognized as an important niche for fungal endophytes and the interaction between them has prompted the invention of novel metabolite. In this regard, the presen...
ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining o... more ABSTRACT According to the United Nations Environment Programme (UNEP), soil health is declining over the decades and it has an adverse impact on human health and food security. Hence, soil health restoration is a need of the hour. It is known that microorganisms play a vital role in remediation of soil pollutants like heavy metals, pesticides, hydrocarbons, etc. However, the indigenous microbes have a limited capacity to degrade these pollutants and it will be a slow process. Genetically modified organisms (GMOs) can catalyze the degradation process as their altered metabolic pathways lead to hypersecretions of various biomolecules that favor the bioremediation process. This review provides an overview on the application of bioengineered microorganisms for the restoration of soil health by degradation of various pollutants. It also sheds light on the challenges of using GMOs in environmental application as their introduction may affect the normal microbial community in soil. Since soil health also refers to the potential of native organisms to survive, the possible changes in the native microbial community with the introduction of GMOs are also discussed. Finally, the future prospects of using bioengineered microorganisms in environmental engineering applications to make the soil fertile and healthy have been deciphered. With the alarming rates of soil health loss, the treatment of soil and soil health restoration need to be fastened to a greater pace and the combinatorial efforts unifying GMOs, plant growth-promoting rhizobacteria, and other soil amendments will provide an effective solution to soil heath restoration ten years ahead. Graphical Abstract Schematic Representation Of Approaches Used To Restore Soil Health
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2021
Traditionally used herbs have been recognized as an important niche for fungal endophytes and the... more Traditionally used herbs have been recognized as an important niche for fungal endophytes and the interaction between them has prompted the invention of novel metabolite. In this regard, the presen...
International Journal of Tropical Insect Science, 2020
Plant extracts prompting free radical formation causing DNA damage among especially symbiotic mic... more Plant extracts prompting free radical formation causing DNA damage among especially symbiotic microorganisms of Aedes aegypti has not been extensively examined in previous studies. Here, investigated whether: (1) it was possible to reduce the vectorial capacity by eliminating the microbial consortia of Aedes aegypti larvae, (2) the excess formation of free radical could induce damages of genomic DNA and alter the morphological appearance of Aedes aegypti larvae, and (3) which are the probable mechanisms driving the death of mosquitoes that has been treated with phytochemicals. The toxicity of Catunaregam spinosa seed extracts on the microbial community of Aedes aegypti larvae was determined as previously described. The formation of free radicals was confirmed using the ammonium molybdate method. While the GCMS method was used to assess the phytochemical analysis and the DNA lysing potential. The petroleum ether, ethanol, acetone and water extracts of Catunaregam spinosa has exhibited potent toxicity towards Aedes aegypti larvea’s gut bacterial microbiota, including Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis with strong MIC values ranging from 0.07 mg/ml to 5.9 mg/ml. Plant extract induced free radical formations and oxidative damage to DNA. The presence of the bioactive element squalene justifies the insecticidal proprieties of Catunaregam spinosa extract. This study reflects the probable mechanisms underlying the lethal effect of Catunaregam spinosa extract on Aedes aegypti, and it potential as a novel biological vector control approach.
The non-conventional yeasts Kluyveromyces lactis, Yarrowia lipolytica, Ogataea polymorpha and Pic... more The non-conventional yeasts Kluyveromyces lactis, Yarrowia lipolytica, Ogataea polymorpha and Pichia pastoris have been developed as eukaryotic expression hosts because of their desirable growth characteristics, including inhibitor and thermo-tolerance, utilization of diverse carbon substrates, and high amount of extracellular protein secretion. These yeasts already have established in the heterologous production of vaccines, therapeutic proteins, food additives, and bio-renewable chemicals, but recent advances in genetic tool box have the potential to greatly expand and diversify their impact on biotechnology. The diversity of these yeasts includes many strains possessing highly useful, and in some cases even uncommon, metabolic capabilities potentially helpful for bioprocess industry. This review outlines the recent updates of non-conventional yeast in sustainable bioprocesses.
Anionic surfactants like sodium dodecyl sulphate (SDS), due to its extensive disposal to water bo... more Anionic surfactants like sodium dodecyl sulphate (SDS), due to its extensive disposal to water bodies cause detrimental effects to the ecosystem. Among the various attempts to reduce the after effects of these toxicants, microbial induced bioremediation serves as a promising strategy. The current study aimed to develop a three stage bioreactor to remediate anionic surfactants in wastewater using effective bacterial isolates. Screening of effective SDS biodegraders led to isolation of Pseudomonas aeruginosa (MTCC 10311). Treatment of synthetic effluent with an immobilized packed bed reactor at a flow rate of 5 mL h−1 resulted in 81 ± 2% SDS eliminations and 70 ± 1% reduction in chemical oxygen demand (COD) in five cycles (6 h per cycle). The hydraulic retention time of the reactor was found to be 6 h. Combinatorial usage of a three stage bioreactor, involving aeration, adsorption with low cost scrap rubber granules and treatment with immobilized Pseudomonas aeruginosa, successfully r...
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Papers by Sharrel Rebello