Objectives: Oral infections and dental caries are still considered as serious public health probl... more Objectives: Oral infections and dental caries are still considered as serious public health problems and inflict a costly burden to health care services around the world and especially in developing countries. Materials and methods: In the present study, we evaluated the antibacterial activity of Glycyrrhiza glabra (G. glabra) against oral pathogens by diffusion methods and determined the minimum inhibitory concentration (MIC) by both broth and Agar dilution methods and minimum bactericidal concentration (MBC) by broth dilution methods. Results: In this study, G. glabra extract showed good antibacterial activity against six bacteria. No strain in this study showed resistance against this extract. Conclusion: G. glabrais suggested as an appropriate candidate to help us in order to control dental caries and endodontic infections.
Oral infections and dental caries are still considered as serious public health problems and infl... more Oral infections and dental caries are still considered as serious public health problems and inflict a costly burden to health care services around the world and especially in developing countries. In the present study, we evaluated the antibacterial activity of Glycyrrhiza glabra (G. glabra) against oral pathogens by diffusion methods and determined the minimum inhibitory concentration (MIC) by both broth and Agar dilution methods and minimum bactericidal concentration (MBC) by broth dilution methods. In this study, G. glabra extract showed good antibacterial activity against six bacteria. No strain in this study showed resistance against this extract. G. glabrais suggested as an appropriate candidate to help us in order to control dental caries and endodontic infections.
Antibacterial activity of Tribulus terrestris and its synergistic effect with Capsella bursa-past... more Antibacterial activity of Tribulus terrestris and its synergistic effect with Capsella bursa-pastoris and Glycyrrhiza glabra against oral pathogens: an in-vitro study
In this study, antimicrobial activities of an ethanol extract of Tribulus terrestris aloneand in ... more In this study, antimicrobial activities of an ethanol extract of Tribulus terrestris aloneand in combination with Capsella bursa-pastoris and Glycyrrhiza glabra were examined in vitro against six pathogens namely Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, Enterococcus faecalis Staphylococcus aureus, and Escherichia coli. Antibacterial activities of the extracts were examined using disc and well diffusion methods and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ethanol extracts were determined against these microorganisms using agar and broth dilution methods. Chlorhexidine was used as positive control. Tribulus terrestris extract exhibited good antibacterial activity against all bacteria. Antibacterial activity of mixed extract was evaluated and exhibited that mixed extract was more effective against all bacteria than any of the cases alone which indicates the synergistic effect between these three extracts (p˂0.0...
Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, e... more Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. The aim of this study was to clone and express the TB10.4 protein in Escherichia coli expression system. Materials and Methods DNA was extracted from Mycobacterium tuberculosis H37Rv. Gene specific primers were designed using Gene Runner software according to sanger sequence database. Gene tb10.4 fragment was amplified by PCR method and purified tb10.4 gene was cloned into pET 102/D vector. Plasmid containing pET102/D-10.4 was transformed into competence E. coli TOP10. A positive transformant was chosen and plasmids DNA was isolated and subsequently transformed into competence E. coli BL21(DE3). The bacterium was induced by IPTG and its lysates were loade...
Background and Objectives: There has been an increasing interest in plant essential oils during r... more Background and Objectives: There has been an increasing interest in plant essential oils during recent years because of the need of new therapies against microbes. Bacterial resistance is spreading throughout the world primarily due to excessive use of antibiotics and poor infection control practices in hospitals, making it one of our times biggest issues. On the other hand, artificial drugs such as mouthwashes, have unpleasant side effects and the number of drug resistant microorganisms is increasing. Finding plants that have antimicrobial effects and using them as mouthwashes, will be decrease the side effects and also they’ll be more economical. In the present study, the antimicrobial property of essential oils of Salvia leriifolia Benth, (Lamiaceae), a native and pharmaceutical plant species of Khorasan province, against bacterial infection causes pharyngitis, was investigated. Materials & Methods: Leaves of S. leriifolia were collected at full flowering stage and essential oils...
Introduction and objective: The early secretory antigenic target 6kDa protein (ESAT-6) antigen fr... more Introduction and objective: The early secretory antigenic target 6kDa protein (ESAT-6) antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients. ESAT-6 was found to distinguish TB patients from BCG-vaccinated donors. The aim of this study was cloning and expression of ESAT-6 of M. tuberculosis. Materials and methods: DNA was extracted from M. tuberculosis H37Rv. PCR was performed using gene-specific oligonucleotide primers and the PCR products were inserted into the pET102/D vector and transferred into Escherichia coli strain TOPO10. The recombinant plasmids transferred into E. coli strain BL21. Results: The transformed plasmid into E. coli strain BL21 was effectively expressed. The expressed fusion protein (23kDa on SDS-PAGE) was found almost entirely in the soluble form and the recombinant protein was purified by Ni-NTA column. Conclusion: We successfully cloned and expressed ESAT-6 protein of M....
Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, e... more Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. . The aim of this study was to clone and express the TB10.4 protein in Escherichia coli expression system. Materials and Methods DNA was extracted from Mycobacterium tuberculosis H37Rv. . Gene specific primers were designed using Gene Runner software according to sanger sequence database. Gene tb10. .4 fragment was amplified by PCR method and purified tb10.4 gene was cloned into pET 102/ /D vector. . Plasmid containing pET102/D-10.4 was transformed into competence E. coli TOP10. A positive transformant was chosen and plasmids DNA was isolated and subsequently transformed into competence E. coli BL21(DE3). The bacterium was induced by IPTG and its lysates ...
Cloning, expression and purification of early secretory antigenic target 6kDa protein (ESAT-6) of... more Cloning, expression and purification of early secretory antigenic target 6kDa protein (ESAT-6) of Mycobacterium tuberculosis. Jundishapur J Microbiol. 2010; 3(2): 53-60. Abstract Introduction and objective: The early secretory antigenic target 6kDa protein (ESAT-6) antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients. ESAT-6 was found to distinguish TB patients from BCG-vaccinated donors. The aim of this study was cloning and expression of ESAT-6 of M. tuberculosis. Materials and methods: DNA was extracted from M. tuberculosis H37Rv. PCR was performed using gene-specific oligonucleotide primers and the PCR products were inserted into the pET102/D vector and transferred into Escherichia coli strain TOPO10. The recombinant plasmids transferred into E. coli strain BL21. Results: The transformed plasmid into E. coli strain BL21 was effectively expressed. The expressed fusion protein (23kDa on SDS-P...
Objectives: Oral infections and dental caries are still considered as serious public health probl... more Objectives: Oral infections and dental caries are still considered as serious public health problems and inflict a costly burden to health care services around the world and especially in developing countries. Materials and methods: In the present study, we evaluated the antibacterial activity of Glycyrrhiza glabra (G. glabra) against oral pathogens by diffusion methods and determined the minimum inhibitory concentration (MIC) by both broth and Agar dilution methods and minimum bactericidal concentration (MBC) by broth dilution methods. Results: In this study, G. glabra extract showed good antibacterial activity against six bacteria. No strain in this study showed resistance against this extract. Conclusion: G. glabrais suggested as an appropriate candidate to help us in order to control dental caries and endodontic infections.
Oral infections and dental caries are still considered as serious public health problems and infl... more Oral infections and dental caries are still considered as serious public health problems and inflict a costly burden to health care services around the world and especially in developing countries. In the present study, we evaluated the antibacterial activity of Glycyrrhiza glabra (G. glabra) against oral pathogens by diffusion methods and determined the minimum inhibitory concentration (MIC) by both broth and Agar dilution methods and minimum bactericidal concentration (MBC) by broth dilution methods. In this study, G. glabra extract showed good antibacterial activity against six bacteria. No strain in this study showed resistance against this extract. G. glabrais suggested as an appropriate candidate to help us in order to control dental caries and endodontic infections.
Antibacterial activity of Tribulus terrestris and its synergistic effect with Capsella bursa-past... more Antibacterial activity of Tribulus terrestris and its synergistic effect with Capsella bursa-pastoris and Glycyrrhiza glabra against oral pathogens: an in-vitro study
In this study, antimicrobial activities of an ethanol extract of Tribulus terrestris aloneand in ... more In this study, antimicrobial activities of an ethanol extract of Tribulus terrestris aloneand in combination with Capsella bursa-pastoris and Glycyrrhiza glabra were examined in vitro against six pathogens namely Streptococcus mutans, Streptococcus sanguis, Actinomyces viscosus, Enterococcus faecalis Staphylococcus aureus, and Escherichia coli. Antibacterial activities of the extracts were examined using disc and well diffusion methods and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ethanol extracts were determined against these microorganisms using agar and broth dilution methods. Chlorhexidine was used as positive control. Tribulus terrestris extract exhibited good antibacterial activity against all bacteria. Antibacterial activity of mixed extract was evaluated and exhibited that mixed extract was more effective against all bacteria than any of the cases alone which indicates the synergistic effect between these three extracts (p˂0.0...
Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, e... more Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. The aim of this study was to clone and express the TB10.4 protein in Escherichia coli expression system. Materials and Methods DNA was extracted from Mycobacterium tuberculosis H37Rv. Gene specific primers were designed using Gene Runner software according to sanger sequence database. Gene tb10.4 fragment was amplified by PCR method and purified tb10.4 gene was cloned into pET 102/D vector. Plasmid containing pET102/D-10.4 was transformed into competence E. coli TOP10. A positive transformant was chosen and plasmids DNA was isolated and subsequently transformed into competence E. coli BL21(DE3). The bacterium was induced by IPTG and its lysates were loade...
Background and Objectives: There has been an increasing interest in plant essential oils during r... more Background and Objectives: There has been an increasing interest in plant essential oils during recent years because of the need of new therapies against microbes. Bacterial resistance is spreading throughout the world primarily due to excessive use of antibiotics and poor infection control practices in hospitals, making it one of our times biggest issues. On the other hand, artificial drugs such as mouthwashes, have unpleasant side effects and the number of drug resistant microorganisms is increasing. Finding plants that have antimicrobial effects and using them as mouthwashes, will be decrease the side effects and also they’ll be more economical. In the present study, the antimicrobial property of essential oils of Salvia leriifolia Benth, (Lamiaceae), a native and pharmaceutical plant species of Khorasan province, against bacterial infection causes pharyngitis, was investigated. Materials & Methods: Leaves of S. leriifolia were collected at full flowering stage and essential oils...
Introduction and objective: The early secretory antigenic target 6kDa protein (ESAT-6) antigen fr... more Introduction and objective: The early secretory antigenic target 6kDa protein (ESAT-6) antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients. ESAT-6 was found to distinguish TB patients from BCG-vaccinated donors. The aim of this study was cloning and expression of ESAT-6 of M. tuberculosis. Materials and methods: DNA was extracted from M. tuberculosis H37Rv. PCR was performed using gene-specific oligonucleotide primers and the PCR products were inserted into the pET102/D vector and transferred into Escherichia coli strain TOPO10. The recombinant plasmids transferred into E. coli strain BL21. Results: The transformed plasmid into E. coli strain BL21 was effectively expressed. The expressed fusion protein (23kDa on SDS-PAGE) was found almost entirely in the soluble form and the recombinant protein was purified by Ni-NTA column. Conclusion: We successfully cloned and expressed ESAT-6 protein of M....
Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, e... more Objective(s) Tuberculosis (TB) is the leading cause of mortality among the infectious diseases, especially in developing countries. One of the main goals in tuberculosis research is to identify antigens which have the ability of inducing cellular and/or humoral immunity in order to use them in diagnostic reagents or vaccine design. . The aim of this study was to clone and express the TB10.4 protein in Escherichia coli expression system. Materials and Methods DNA was extracted from Mycobacterium tuberculosis H37Rv. . Gene specific primers were designed using Gene Runner software according to sanger sequence database. Gene tb10. .4 fragment was amplified by PCR method and purified tb10.4 gene was cloned into pET 102/ /D vector. . Plasmid containing pET102/D-10.4 was transformed into competence E. coli TOP10. A positive transformant was chosen and plasmids DNA was isolated and subsequently transformed into competence E. coli BL21(DE3). The bacterium was induced by IPTG and its lysates ...
Cloning, expression and purification of early secretory antigenic target 6kDa protein (ESAT-6) of... more Cloning, expression and purification of early secretory antigenic target 6kDa protein (ESAT-6) of Mycobacterium tuberculosis. Jundishapur J Microbiol. 2010; 3(2): 53-60. Abstract Introduction and objective: The early secretory antigenic target 6kDa protein (ESAT-6) antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients. ESAT-6 was found to distinguish TB patients from BCG-vaccinated donors. The aim of this study was cloning and expression of ESAT-6 of M. tuberculosis. Materials and methods: DNA was extracted from M. tuberculosis H37Rv. PCR was performed using gene-specific oligonucleotide primers and the PCR products were inserted into the pET102/D vector and transferred into Escherichia coli strain TOPO10. The recombinant plasmids transferred into E. coli strain BL21. Results: The transformed plasmid into E. coli strain BL21 was effectively expressed. The expressed fusion protein (23kDa on SDS-P...
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