Biomedic

Abstract: Antioxidant has been the important approaches to reduce the development of cancer disease and play a role as a protective against liver toxicity. Channastriatus (haruan) have been used traditionally oral remedy for wound healing among women after child birth. However, there is little scientific evidence or research yet regarding the cytotoxicity and hepatoprotective activity of C.striatus. Thus, the aims of this study are to determine potential total phenolic content (TPC), antioxidant, cytotoxicity and hepatoprotective effect of C.striatus (Haruan) extract. For this present study, aqueous and lipid extract of was prepared using chloroform and methanol solvent in a ratio of 2:1. Folin-Ciocalteu was used to quantify TPC and three antioxidant assays were used to determine antioxidant activity, including 2,2-diphenyl-picrylhydrazyl (DPPH) assay, azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and ferric reducing ability of power (FRAP). For cell viability assay, HepG...

The purposes of this study were to determine total phenolic content (TPC) and antioxidant activities of C.capsularis and S.rebaudiana. Methodology: C.capsularis was extracted successively with methanol and chloroform methanol separately while S.rebaudiana was extracted using methanol and chloroform solvent. The plant extracts were used to conduct various antioxidant assays. TPC assay using Folin-Ciocalteu method was used to assess the presence of phenolic compounds in each sample. The extracts samples were also subjected to assess their potential antioxidant activities through DPPH, ABTS and FRAP assay. The present study showed that both methanol extract of C.capsularis and S.rebaudiana had highest TPC, followed by chloroform extract of S.rebaudiana and chloroform methanol extract of C.capsularis. Next, the DPPH radical scavenging assay was found to be higher in the methanol extract of S.rebaudiana at the concentration of 154.67μg/ml. The cation radical scavenging activity, measured...