Reported studies have showed that Thrombin Activatable Fibrinolysis Inhibitor (TAFI) may be associated with an increased risk of venous thromboembolism. But the relation of VT with TAFI gene SNPs could not be clearly demonstrated. Thus,... more
Reported studies have showed that Thrombin Activatable Fibrinolysis Inhibitor (TAFI) may be associated with an increased risk of venous thromboembolism. But the relation of VT with TAFI gene SNPs could not be clearly demonstrated. Thus, we conducted a meta-analysis to analyze the associations between three TAFI variants -438G/A, 505G/A and 1040C/T and the risk of venous thrombosis. We carried out a systematic search to obtain all the eligible studies published before 30th October 2014. Pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) were computed to assess the association. 13 eligible studies were enrolled including 2321 patients and 2464 controls. There was a significant association between 505G/A and the risk of VTD under all models except recessive model (G vs. A: OR=1.13, 95% CI: 1.02, 1.26; GG vs. AA:OR=1.47, 95% CI: 1.14, 1.88; GA vs. AA: OR=1.36, 95% CI: 1.06, 1.73; GG+GA vs. AA: OR=1.41, 95% CI: 1.12, 1.77). Similarly, obvious relationship was observed in sub...
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ABSTRACT Gardenia jasminoides Ellis is an evergreen tropical plant and favorite to gardeners throughout the world. Several studies have documented that in vitro micropropagation can be used for clonal propagation of G. jasminoides Ellis,... more
ABSTRACT Gardenia jasminoides Ellis is an evergreen tropical plant and favorite to gardeners throughout the world. Several studies have documented that in vitro micropropagation can be used for clonal propagation of G. jasminoides Ellis, the efficiency remained low. In addition, no information is available on the genetic and epigenetic fidelity of the micropropagated plants. Here, we report on a simplified protocol for high efficient micropropagation of G. jasminoides Ellis cv. “Kinberly” based on enhanced branching of shoot-tips as explants. The protocol consisted of sequential use of three media, namely, bud-induction, elongation and root-induction. By using two molecular markers, amplified fragment length polymorphism (AFLP) and methylation sensitive amplified polymorphism (MSAP), we analyzed the genetic and DNA methylation pattern stability of 23 morphologically normal plants randomly taken from a sub-population (>100) of micropropagated plants originated from a single shoot-tip. We found that of >1,000 scored AFLP bands across the 23 micropropagated plants, no incident of genetic variation was detected. In contrast, of 750 scored MSAP bands, moderate but clear alteration in several DNA methylation patterns occurred in the majority of the 23 micropropagated plants. The changed methylation patterns involved both CG and CHG sites representing either hyper- or hypo-methylation, which occurred without altering the total methylation levels partly due to concomitant hyper- and hypo-methylation alterations. Our results indicated that epigenetic instability in the form of DNA methylation patterns can be susceptible to the in vitro micropropagation process for G. jasminoides Ellis, and needs to be taken into account in the process of large-scale commercial propagation of this plant.
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Six one-third scale reinforced concrete (RC) beam-column substructures, each with different design detail, span length and span aspect ratio, were designed and tested to investigate the dynamic load redistribution performance of RC... more
Six one-third scale reinforced concrete (RC) beam-column substructures, each with different design detail, span length and span aspect ratio, were designed and tested to investigate the dynamic load redistribution performance of RC substructures following predefined initial damage. The initial damage was caused by the sudden removal of the corner support. The tested variables include: the longitudinal and transverse reinforcement ratio in the beams, columns and joints, design span length and span aspect ratio. Extensive instrumentation was installed in predetermined locations on the tested specimens prior to conducting the tests, recording data such as acceleration, velocity and displacement distribution. The histories of the bending moments, as well as the vertical and horizontal reaction forces at the fixed supports were also noted. The test results indicated that the column removal apparatus was effective and confirmed that the design span length significantly affected the capability of the frames to resist progressive collapse. Furthermore, it was demonstrated that seismically detailed specimens could mount a more robust performance in resisting progressive collapse.► Investigation of dynamic performance of RC substructures after predefined damage. ► Variables include reinforcement ratio and span aspect ratio. ► Design span length affects capability to resist progressive collapse.