Elangovan M, Rai R, Dholakia BB, Lagu MD, Tiwari R, Gupta RK, Rao VS, ROder MS and Gupta VS Plant... more Elangovan M, Rai R, Dholakia BB, Lagu MD, Tiwari R, Gupta RK, Rao VS, ROder MS and Gupta VS Plant Molecular Biology Unit, Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India. Directorate of Wheat Research, Karnal 132001, India. Genetics and Plant Breeding Unit, Plant Sciences Division, Agharkar Research Institute, Pune 411004, India. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Pucci... more Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Puccinia triticina Rob. ex Desm. are major constraints to wheat production worldwide. In the present study, F(4)-derived SSD population, developed from a cross between Australian cultivars 'Schomburgk' and 'Yarralinka', was used to identify molecular markers linked to rust resistance genes Lr 3 a and Sr 22. A total of 1,330 RAPD and 100 ISSR primers and 33 SSR primer pairs selected ob the basis of chromosomal locations of these genes were used. The ISSR marker UBC 840(540) was found to be linked with Lr 3 a in repulsion at a distance of 6.0 cM. Markers cfa 2019 and cfa 2123 flanked Sr 22 at a distance of 5.9 cM (distal) and 6.0 cM (proximal), respectively. The use of these markers in combination would predict the presence or absence of Sr 22 in breeding populations. A previously identified PCR-based diagnostic marker STS 638 linked to Lr 20 was validated in this population. This marker showed a recombination value of 7.1 cM with Lr 20.
Abstract The economic value of wheat grain is determined by the kernel morphology which is an imp... more Abstract The economic value of wheat grain is determined by the kernel morphology which is an important parameter for manufacturing different food products requiring specific grain characteristics. Although kernel size and shape have emerged as important breeding ...
Yellow berry (YB) is a serious seed disorder in durum wheat, bread wheatand triticale, which aris... more Yellow berry (YB) is a serious seed disorder in durum wheat, bread wheatand triticale, which arises due to deficiency in nitrogen concentration in thesoil. YB seriously affects the grain protein content (GPC) thereby affectingbread making quality in bread wheat and pasta making quality in durumwheat. In order to study the inheritance and to identify DNA markersassociated with YB tolerance, a
Figure S2. Heatmap depicting expression profiles of 2262 hierarchically clustered proteins from d... more Figure S2. Heatmap depicting expression profiles of 2262 hierarchically clustered proteins from different stages of C. grandis flower bud samples. AGE (Red), Early-staged Ag-H; AGM (Green), Middle-staged Ag-H; FE (Blue), Early-staged Female; FM (Turquoise), Middle-staged Female; GE (Pink), Early-staged GyM-H; GM (Yellow), Middle-staged GyM-H; ME (Grey), Early-staged Male; MM (Black), Middle-staged Male. (TIF 56 kb)
Figure S1. Enzyme code distribution analysis for the detected C. grandis flower bud proteins usin... more Figure S1. Enzyme code distribution analysis for the detected C. grandis flower bud proteins using BLAST2GO v5. (TIF 1214 kb)
Data S2. BLAST2GO annotation table for the C. grandis flower bud proteins detected in this study.... more Data S2. BLAST2GO annotation table for the C. grandis flower bud proteins detected in this study. (XLSX 640 kb)
Data S1. Detailed report of protein identification from C. grandis flower buds using Paragon algo... more Data S1. Detailed report of protein identification from C. grandis flower buds using Paragon algorithm with ProteinPilot v5.0.1. (XLSX 12245 kb)
Data S5. Overlap of differentially expressed proteins between all the pairwise comparisons at mid... more Data S5. Overlap of differentially expressed proteins between all the pairwise comparisons at middle stage of flower development. (XLSX 38 kb)
Data S4. Overlap of differentially expressed proteins between all the pairwise comparisons at ear... more Data S4. Overlap of differentially expressed proteins between all the pairwise comparisons at early stage of flower development. (XLSX 42 kb)
Figure S3. Venn diagram showing the overlap of differentially expressed proteins between all the ... more Figure S3. Venn diagram showing the overlap of differentially expressed proteins between all the pairwise comparisons at early (A) and middle stages (B) of flower development. (PDF 343 kb)
Data S3. Normalized peak areas for each of the quantified proteins across all the samples and pai... more Data S3. Normalized peak areas for each of the quantified proteins across all the samples and pairwise differential expression analysis results with respective fold-changes as well as P-values. DEPs with fold change ≥1.5 and P ≤ 0.05 are shown in highlighted cells for all pairwise comparisons. (XLSX 7759 kb)
Data S6. Normalized peak areas for the proteins involved in male and female reproductive organ de... more Data S6. Normalized peak areas for the proteins involved in male and female reproductive organ development, seed development and embryo development along with their respective Arabidopsis accession IDs. (XLSX 144 kb)
Plant Cell, Tissue and Organ Culture (PCTOC), 2017
Ceropegia karulensis is an endemic and critically endangered plant of the Western Ghats from Indi... more Ceropegia karulensis is an endemic and critically endangered plant of the Western Ghats from India. Exploitation of the tubers and poor regeneration from seed has narrowed distribution and propagation of the species. There is a need to develop in vitro propagation methods for C. karulensis to alleviate these problems. Here, we optimized callus induction, somatic embryogenesis and microtuberization from different seedling explants viz. cotyledonary leaf and root. The environmental scanning electron microscopy was used to observe somatic embryonic origin and their developmental stages. Highest callus proliferation was recorded with 2 µM 6-benzylaminopurine and 1 µM 2,4-dichlorophenoxyacetic acid. Somatic embryos derived from cotyledonary leaf explants were more proliferative than root explants. The combination of 2 µM 6-benzylaminopurine, 2 µM naphthalene acetic acid and 7% sucrose in MS media resulted in highest microtuberization. Further, gas chromatography-mass spectrometry based metabolic profiling was carried out from native wild plants and in vitro callus tissues which identified various phytochemicals such as alkaloids, fatty acids, esters alcohols, etc. Multivariate analysis revealed the chemical disparities, where considerable variations were observed between native wild type and in vitro tissues, but no significant differences were found among in vitro callus from both root and cotyledonary explants. Overall, our results suggested that the production of various secondary metabolites found in C. karulensis was not affected by in vitro propagation and could be utilized in the conservation strategies for this plant.Graphical Abstract
Isoprenoids and phenylpropanoids are the major secondary metabolite constituents in Ocimum genus.... more Isoprenoids and phenylpropanoids are the major secondary metabolite constituents in Ocimum genus. Though enzymes from phenylpropanoid pathway have been characterized from few plants, limited information exists on how they modulate levels of secondary metabolites. Here, we performed phenylpropanoid profiling in different tissues from five Ocimum species, which revealed significant variations in secondary metabolites including eugenol, eugenol methyl ether, estragole and methyl cinnamate levels. Expression analysis of eugenol synthase (EGS) gene showed higher transcript levels especially in young leaves and inflorescence; and were positively correlated with eugenol contents. Additionally, transcript levels of coniferyl alcohol acyl transferase, a key enzyme diverting pool of substrate to phenylpropanoids, were in accordance with their abundance in respective species. In particular, eugenol methyl transferase expression positively correlated with higher levels of eugenol methyl ether i...
ABSTRACT Yellow berry (YB) is a physiological kernel disorder in wheat, which manifests as yellow... more ABSTRACT Yellow berry (YB) is a physiological kernel disorder in wheat, which manifests as yellow, mottled, non-vitreous and starchy kernels. It occurs due to deficient soil nitrogen levels and affects milling and end-product quality of wheat. Genetic control of this disorder and association of some of the chromosomes with YB tolerance has been shown previously. However, the location of genes associated with YB tolerance on such chromosomes was not known. In view of this, we developed a simple sequence repeat marker based linkage map of hexaploid wheat using a recombinant inbred line population that segregated for YB tolerance and mapped the YB-linked genes on two wheat chromosomes. A major gene associated with YB was mapped on the short arm of chromosome 5D. In addition, a minor locus on chromosome 2D was detected that showed 6.9% contribution to the phenotypic variance in YB. The markers associated with the major gene for YB could be used for developing better varieties of wheat by molecular breeding.
Elangovan M, Rai R, Dholakia BB, Lagu MD, Tiwari R, Gupta RK, Rao VS, ROder MS and Gupta VS Plant... more Elangovan M, Rai R, Dholakia BB, Lagu MD, Tiwari R, Gupta RK, Rao VS, ROder MS and Gupta VS Plant Molecular Biology Unit, Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India. Directorate of Wheat Research, Karnal 132001, India. Genetics and Plant Breeding Unit, Plant Sciences Division, Agharkar Research Institute, Pune 411004, India. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Pucci... more Stem rust caused by Puccinia graminis f. sp. tritici Eriks and Henn and leaf rust caused by Puccinia triticina Rob. ex Desm. are major constraints to wheat production worldwide. In the present study, F(4)-derived SSD population, developed from a cross between Australian cultivars 'Schomburgk' and 'Yarralinka', was used to identify molecular markers linked to rust resistance genes Lr 3 a and Sr 22. A total of 1,330 RAPD and 100 ISSR primers and 33 SSR primer pairs selected ob the basis of chromosomal locations of these genes were used. The ISSR marker UBC 840(540) was found to be linked with Lr 3 a in repulsion at a distance of 6.0 cM. Markers cfa 2019 and cfa 2123 flanked Sr 22 at a distance of 5.9 cM (distal) and 6.0 cM (proximal), respectively. The use of these markers in combination would predict the presence or absence of Sr 22 in breeding populations. A previously identified PCR-based diagnostic marker STS 638 linked to Lr 20 was validated in this population. This marker showed a recombination value of 7.1 cM with Lr 20.
Abstract The economic value of wheat grain is determined by the kernel morphology which is an imp... more Abstract The economic value of wheat grain is determined by the kernel morphology which is an important parameter for manufacturing different food products requiring specific grain characteristics. Although kernel size and shape have emerged as important breeding ...
Yellow berry (YB) is a serious seed disorder in durum wheat, bread wheatand triticale, which aris... more Yellow berry (YB) is a serious seed disorder in durum wheat, bread wheatand triticale, which arises due to deficiency in nitrogen concentration in thesoil. YB seriously affects the grain protein content (GPC) thereby affectingbread making quality in bread wheat and pasta making quality in durumwheat. In order to study the inheritance and to identify DNA markersassociated with YB tolerance, a
Figure S2. Heatmap depicting expression profiles of 2262 hierarchically clustered proteins from d... more Figure S2. Heatmap depicting expression profiles of 2262 hierarchically clustered proteins from different stages of C. grandis flower bud samples. AGE (Red), Early-staged Ag-H; AGM (Green), Middle-staged Ag-H; FE (Blue), Early-staged Female; FM (Turquoise), Middle-staged Female; GE (Pink), Early-staged GyM-H; GM (Yellow), Middle-staged GyM-H; ME (Grey), Early-staged Male; MM (Black), Middle-staged Male. (TIF 56 kb)
Figure S1. Enzyme code distribution analysis for the detected C. grandis flower bud proteins usin... more Figure S1. Enzyme code distribution analysis for the detected C. grandis flower bud proteins using BLAST2GO v5. (TIF 1214 kb)
Data S2. BLAST2GO annotation table for the C. grandis flower bud proteins detected in this study.... more Data S2. BLAST2GO annotation table for the C. grandis flower bud proteins detected in this study. (XLSX 640 kb)
Data S1. Detailed report of protein identification from C. grandis flower buds using Paragon algo... more Data S1. Detailed report of protein identification from C. grandis flower buds using Paragon algorithm with ProteinPilot v5.0.1. (XLSX 12245 kb)
Data S5. Overlap of differentially expressed proteins between all the pairwise comparisons at mid... more Data S5. Overlap of differentially expressed proteins between all the pairwise comparisons at middle stage of flower development. (XLSX 38 kb)
Data S4. Overlap of differentially expressed proteins between all the pairwise comparisons at ear... more Data S4. Overlap of differentially expressed proteins between all the pairwise comparisons at early stage of flower development. (XLSX 42 kb)
Figure S3. Venn diagram showing the overlap of differentially expressed proteins between all the ... more Figure S3. Venn diagram showing the overlap of differentially expressed proteins between all the pairwise comparisons at early (A) and middle stages (B) of flower development. (PDF 343 kb)
Data S3. Normalized peak areas for each of the quantified proteins across all the samples and pai... more Data S3. Normalized peak areas for each of the quantified proteins across all the samples and pairwise differential expression analysis results with respective fold-changes as well as P-values. DEPs with fold change ≥1.5 and P ≤ 0.05 are shown in highlighted cells for all pairwise comparisons. (XLSX 7759 kb)
Data S6. Normalized peak areas for the proteins involved in male and female reproductive organ de... more Data S6. Normalized peak areas for the proteins involved in male and female reproductive organ development, seed development and embryo development along with their respective Arabidopsis accession IDs. (XLSX 144 kb)
Plant Cell, Tissue and Organ Culture (PCTOC), 2017
Ceropegia karulensis is an endemic and critically endangered plant of the Western Ghats from Indi... more Ceropegia karulensis is an endemic and critically endangered plant of the Western Ghats from India. Exploitation of the tubers and poor regeneration from seed has narrowed distribution and propagation of the species. There is a need to develop in vitro propagation methods for C. karulensis to alleviate these problems. Here, we optimized callus induction, somatic embryogenesis and microtuberization from different seedling explants viz. cotyledonary leaf and root. The environmental scanning electron microscopy was used to observe somatic embryonic origin and their developmental stages. Highest callus proliferation was recorded with 2 µM 6-benzylaminopurine and 1 µM 2,4-dichlorophenoxyacetic acid. Somatic embryos derived from cotyledonary leaf explants were more proliferative than root explants. The combination of 2 µM 6-benzylaminopurine, 2 µM naphthalene acetic acid and 7% sucrose in MS media resulted in highest microtuberization. Further, gas chromatography-mass spectrometry based metabolic profiling was carried out from native wild plants and in vitro callus tissues which identified various phytochemicals such as alkaloids, fatty acids, esters alcohols, etc. Multivariate analysis revealed the chemical disparities, where considerable variations were observed between native wild type and in vitro tissues, but no significant differences were found among in vitro callus from both root and cotyledonary explants. Overall, our results suggested that the production of various secondary metabolites found in C. karulensis was not affected by in vitro propagation and could be utilized in the conservation strategies for this plant.Graphical Abstract
Isoprenoids and phenylpropanoids are the major secondary metabolite constituents in Ocimum genus.... more Isoprenoids and phenylpropanoids are the major secondary metabolite constituents in Ocimum genus. Though enzymes from phenylpropanoid pathway have been characterized from few plants, limited information exists on how they modulate levels of secondary metabolites. Here, we performed phenylpropanoid profiling in different tissues from five Ocimum species, which revealed significant variations in secondary metabolites including eugenol, eugenol methyl ether, estragole and methyl cinnamate levels. Expression analysis of eugenol synthase (EGS) gene showed higher transcript levels especially in young leaves and inflorescence; and were positively correlated with eugenol contents. Additionally, transcript levels of coniferyl alcohol acyl transferase, a key enzyme diverting pool of substrate to phenylpropanoids, were in accordance with their abundance in respective species. In particular, eugenol methyl transferase expression positively correlated with higher levels of eugenol methyl ether i...
ABSTRACT Yellow berry (YB) is a physiological kernel disorder in wheat, which manifests as yellow... more ABSTRACT Yellow berry (YB) is a physiological kernel disorder in wheat, which manifests as yellow, mottled, non-vitreous and starchy kernels. It occurs due to deficient soil nitrogen levels and affects milling and end-product quality of wheat. Genetic control of this disorder and association of some of the chromosomes with YB tolerance has been shown previously. However, the location of genes associated with YB tolerance on such chromosomes was not known. In view of this, we developed a simple sequence repeat marker based linkage map of hexaploid wheat using a recombinant inbred line population that segregated for YB tolerance and mapped the YB-linked genes on two wheat chromosomes. A major gene associated with YB was mapped on the short arm of chromosome 5D. In addition, a minor locus on chromosome 2D was detected that showed 6.9% contribution to the phenotypic variance in YB. The markers associated with the major gene for YB could be used for developing better varieties of wheat by molecular breeding.
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