In this study, the efficiency of the ceftiofur N-acyl homoserine lactonase niosome against multi-... more In this study, the efficiency of the ceftiofur N-acyl homoserine lactonase niosome against multi-resistant Klebsiella pneumoniae in broilers was evaluated. Fifty-six K. pneumoniae isolates previously recovered from different poultry and environmental samples were screened for the ahlK gene. The lactonase enzyme was extracted from eight quorum-quenching isolates. The niosome was formulated, characterized, and tested for minimal inhibitory concentration (MIC) and cytotoxicity. Fourteen-day-old chicks were assigned to six groups: groups Ӏ and П served as negative and positive controls, receiving saline and K. pneumoniae solutions, respectively. In groups Ш and IV, ceftiofur and niosome were administrated intramuscularly at a dose of 10 mg/kg body weight for five consecutive days, while groups V and VI received the injections following the K. pneumoniae challenge. Signs, mortality, and gross lesions were recorded. Tracheal swabs were collected from groups П, V, and VI for counting K. pn...
E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections i... more E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections in poultry causing dramatic economic losses in poultry (Gast and Porter, 2020; Nolan et al., 2020). Moreover, poultry is one of the most commonly used sources of animal protein in the Egyptian market, and therefore it may act as a potential research Article Abstract | The present research investigated the efficacy of amoxicillin and enrofloxacin when being used alone, in a half-dose combination, and in a full-dose combination against Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was infected with the mixed infection and treated with enrofloxacin 10 mg/kg b.wt. Group five was infected and treated with a full treatment dose of amoxicillin and enrofloxacin. Group six was infected and treated with a half-dose combination of amoxicillin and enrofloxacin. Group seven was non-infected and treated with a full-dose combination of amoxicillin and enrofloxacin. Antimicrobial sensitivity test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combination of enrofloxacin and amoxicillin is safer and more efficient in the treatment of Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens than the usage of these antibiotics in a full-dose combination or solely.
E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections i... more E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections in poultry causing dramatic economic losses in poultry (Gast and Porter, 2020; Nolan et al., 2020). Moreover, poultry is one of the most commonly used sources of animal protein in the Egyptian market, and therefore it may act as a potential research Article Abstract | The present research investigated the efficacy of amoxicillin and enrofloxacin when being used alone, in a half-dose combination, and in a full-dose combination against Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was infected with the mixed infection and treated with enrofloxacin 10 mg/kg b.wt. Group five was infected and treated with a full treatment dose of amoxicillin and enrofloxacin. Group six was infected and treated with a half-dose combination of amoxicillin and enrofloxacin. Group seven was non-infected and treated with a full-dose combination of amoxicillin and enrofloxacin. Antimicrobial sensitivity test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combination of enrofloxacin and amoxicillin is safer and more efficient in the treatment of Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens than the usage of these antibiotics in a full-dose combination or solely.
Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal ... more Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal have generated a great deal of interest in recent years, including medical applications. A novel, specific, precise, accurate and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated to quantify copper (Cu) and copper nanoparticles (CuNPs) in different biological matrices and pharmaceutical products. Methods The developed method has been validated for linearity, precision, sensitivity, specificity and accuracy. Cu concentration was detected in pharmaceutical products without an extraction process. Moreover, liver, serum and muscle tissues were used as biological matrices. High Cu recovery in biological samples was afforded by using citric acid as a green chelating agent, exact extraction time and pH adjustment. Cu pharmaceutical and biological samples were eluted by acetonitrile: ammonium acetate (50 mM) with 0...
Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal ... more Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal have generated a great deal of interest in recent years, including medical applications. A novel, specific, precise, accurate and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated to quantify copper (Cu) and copper nanoparticles (CuNPs) in different biological matrices and pharmaceutical products. Methods The developed method has been validated for linearity, precision, sensitivity, specificity and accuracy. Cu concentration was detected in pharmaceutical products without an extraction process. Moreover, liver, serum and muscle tissues were used as biological matrices. High Cu recovery in biological samples was afforded by using citric acid as a green chelating agent, exact extraction time and pH adjustment. Cu pharmaceutical and biological samples were eluted by acetonitrile: ammonium acetate (50 mM) with 0...
In this study, the prevalence of Salmonella typhimurium in Egyptian chicken farms was studied. Ex... more In this study, the prevalence of Salmonella typhimurium in Egyptian chicken farms was studied. Examination of 79 chicken farms distributed in Egypt governorates from 2017 to 2019. Salmonella was detected in broiler flocks 20 % positivity (18 out of 90). However, in layer flocks showed 70 % positivity (7 out of 10). Then Salmonella typhimurium was confirmed using Salmonella typhimurium STM4 495 gene using conventional PCR. Lipopolysaccharides (LPSs) are the major constituent of the bacterial Gram-negative outer membrane including Salmonella. (LPSs) is an immunodominant molecule that is important for the virulence and pathogenesis of Salmonella typhimurium which called endotoxin. Extraction and purification of LPS from isolated S. typhimurium using isopropanol and sodium hydroxide mixture this overcome the presence of nucleic acids of extracted Salmonella in the final product. The purity of LPS isolated from Salmonella typhimurium was assessed by HPLC showing highly purified extracted...
The current study was conducted on multiple oral doses (40 mg/kg for 3 successive days) of florfe... more The current study was conducted on multiple oral doses (40 mg/kg for 3 successive days) of florfenicol (FF) to determine its effect on some hematological parameters, cardiac enzymes and its residues in blood and tissues (liver, muscles and kidneys) of broiler chickens. Seventy broiler chickens were used and divided into two groups, each consisted of 35 birds. The first group was left as a control, while the second was given FF for 3 successive days. The FF residues in tissues were determined using reversed phase-high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector at 223 nm. Results indicated a widespread distribution of FF in most of the tested tissues. All tissue samples were considered FF free on the 9 th day after the last oral dose except liver. Florfenicol administration elicited a significant decrease in all blood parameters (hemoglobin concentration (Hb), red blood cell count (RBCs), mean corpuscular volume (MCV), mean corpuscular hemoglobin concen...
An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for... more An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantitation of doxycycline in turkey serum has been developed and validated. The doxycycline standard was eluted from a C18 column (4.6 mm i.d., 250 mm, 5 μm particle size) at 15 0C with a mobile phase consisting of 5% acetic acid : methanol : acetonitrile (55:20:25, v/v/v). A UV detector set at 347 nm was used to monitor the effluent. Retention time of doxycycline was approximately at 4 min and the run time was 10 min. The column temperature adjusted at 15 0C with a flow rate 1ml/min and injection volume 25 μl. Doxycycline was extracted from serum by ACN. Linearity was confirmed in the concentration range 0.1-2 μg/ml, had regression coefficients (R 2 ) 0.9999 for doxycycline quantification in turkey serum. The analytical conditions gave a recoveries in a range of 97.57% to 99.83%. The limit of detection (LOD) was 0.035 μg/ mL and limit of quantification (LOQ) was 0.108 μg/ml. The ...
Effect of extracted Lipopolysaccharides of Salmonella typhimurium on chicken immunity Ashraf A. A... more Effect of extracted Lipopolysaccharides of Salmonella typhimurium on chicken immunity Ashraf A. Abd El-Tawab1, Heba M. Hassan2, Fatma I. El-Hofy1, Mai A. Fadel2, Aml Youssef2, Enas A. Soliman1 Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt. 2 Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, P.O. Box 246, Giza 12618, Egypt
This study aimed to detect and identify the N-acyl-homoserine lactones molecules (AHLs) produced ... more This study aimed to detect and identify the N-acyl-homoserine lactones molecules (AHLs) produced by different resistant Klebsiella pneumoniae isolates recovered from poultry and environmental samples using a modified validated high-performance liquid chromatography method. A total of 56 K. pneumoniae isolates were recovered, investigated for their antibiotic susceptibility, and screened for AHLs production using the Agrobacterium tumefaciens NTL4 biosensor system and a validated high-performance liquid chromatography method. The results revealed the detection of different short- and long-chain AHLs molecules among 39 K. pneumoniae isolates recovered from poultry and environmental samples. All environmental isolates produced nine peaks with retention times for C4-HSL, C6-HSL, C12-HSL, C8-HSL, C14-HSL, C8-oxo-HSL, C10-HSL, C6-oxo-HSL, and C7-HSL. The most quantifiable AHL signal molecules in poultry isolates were C4-HSL, C6-HSL, and C12-HSL. No statistical correlation between the AHL-producing ability of K. pneumoniae isolates and antibiotic resistance was reported. To the best of our knowledge, this study provides the first detailed report on the detection and identification of AHLs in K. pneumoniae isolates recovered from poultry and environmental samples. Furthermore, it provides a new insight available tool other than LC-MS/MS for detection and identification of AHL molecules.
Background and Aim: Lipopolysaccharide (LPS) is an integral part of the outer cell membrane compl... more Background and Aim: Lipopolysaccharide (LPS) is an integral part of the outer cell membrane complex of Gram-negative bacteria. It plays an important role in the induction and stimulation of the immune system. Various LPS purification protocols have been developed. However, analysis of their efficacy is limited by contamination during downstream applications or the public health hazard of LPS. The aim of this study was to evaluate a modified method for extracting LPS as well as assess the purity of the extracted LPS by high-performance liquid chromatography (HPLC) analysis. Further, we evaluated its immunopotentiating function by measuring the relative RNA expression levels of splenic immune-related genes such as interleukin 1β (IL-1β) and interferon-γ (IFN-γ), after intramuscular injection of increasing concentrations of the extracted LPS in specific pathogen-free (SPF) chick. Materials and Methods: Isolation, identification, and serotyping of Salmonella Typhimurium were performed u...
Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, per... more Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, performance, and meat quality while reducing public health concern. Influence of NS and inorganic selenium (Se) water additive on performance, carcass quality, immunoglobulin concentration, intestinal microbiota, Se tissue concentrations, and tissue architecture was studied. Materials and Methods: Two-hundred and sixty 1-day-old Hubbard chicks were randomly grouped into five groups (5×52) and supplemented with 0.5 and 1.0 mL of NS and inorganic Se (100 mg.L-1). G1, G2, G3, and G4 were challenged with Escherichia coli O157: H7 2.6×108 on the 14th day. A total of 2250 samples, including 250 sera, 250 intestinal swabs, and 1500 organ and tissue samples as liver, spleen, heart, bursa, intestine, and breast muscles, and 250 eviscerated carcasses were collected. Results: The results revealed a highly significant increase (p<0.01) in live body weights, weight gains, performance indices, carcass...
Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, per... more Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, performance, and meat quality while reducing public health concern. Influence of NS and inorganic selenium (Se) water additive on performance, carcass quality, immunoglobulin concentration, intestinal microbiota, Se tissue concentrations, and tissue architecture was studied. Materials and Methods: Two-hundred and sixty 1-day-old Hubbard chicks were randomly grouped into five groups (5×52) and supplemented with 0.5 and 1.0 mL of NS and inorganic Se (100 mg.L-1). G1, G2, G3, and G4 were challenged with Escherichia coli O157: H7 2.6×108 on the 14th day. A total of 2250 samples, including 250 sera, 250 intestinal swabs, and 1500 organ and tissue samples as liver, spleen, heart, bursa, intestine, and breast muscles, and 250 eviscerated carcasses were collected. Results: The results revealed a highly significant increase (p<0.01) in live body weights, weight gains, performance indices, carcass...
In this study, the efficiency of the ceftiofur N-acyl homoserine lactonase niosome against multi-... more In this study, the efficiency of the ceftiofur N-acyl homoserine lactonase niosome against multi-resistant Klebsiella pneumoniae in broilers was evaluated. Fifty-six K. pneumoniae isolates previously recovered from different poultry and environmental samples were screened for the ahlK gene. The lactonase enzyme was extracted from eight quorum-quenching isolates. The niosome was formulated, characterized, and tested for minimal inhibitory concentration (MIC) and cytotoxicity. Fourteen-day-old chicks were assigned to six groups: groups Ӏ and П served as negative and positive controls, receiving saline and K. pneumoniae solutions, respectively. In groups Ш and IV, ceftiofur and niosome were administrated intramuscularly at a dose of 10 mg/kg body weight for five consecutive days, while groups V and VI received the injections following the K. pneumoniae challenge. Signs, mortality, and gross lesions were recorded. Tracheal swabs were collected from groups П, V, and VI for counting K. pn...
E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections i... more E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections in poultry causing dramatic economic losses in poultry (Gast and Porter, 2020; Nolan et al., 2020). Moreover, poultry is one of the most commonly used sources of animal protein in the Egyptian market, and therefore it may act as a potential research Article Abstract | The present research investigated the efficacy of amoxicillin and enrofloxacin when being used alone, in a half-dose combination, and in a full-dose combination against Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was infected with the mixed infection and treated with enrofloxacin 10 mg/kg b.wt. Group five was infected and treated with a full treatment dose of amoxicillin and enrofloxacin. Group six was infected and treated with a half-dose combination of amoxicillin and enrofloxacin. Group seven was non-infected and treated with a full-dose combination of amoxicillin and enrofloxacin. Antimicrobial sensitivity test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combination of enrofloxacin and amoxicillin is safer and more efficient in the treatment of Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens than the usage of these antibiotics in a full-dose combination or solely.
E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections i... more E scherichia coli and Salmonella enteritidis are the most worldwide spread bacterial infections in poultry causing dramatic economic losses in poultry (Gast and Porter, 2020; Nolan et al., 2020). Moreover, poultry is one of the most commonly used sources of animal protein in the Egyptian market, and therefore it may act as a potential research Article Abstract | The present research investigated the efficacy of amoxicillin and enrofloxacin when being used alone, in a half-dose combination, and in a full-dose combination against Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was infected with the mixed infection and treated with enrofloxacin 10 mg/kg b.wt. Group five was infected and treated with a full treatment dose of amoxicillin and enrofloxacin. Group six was infected and treated with a half-dose combination of amoxicillin and enrofloxacin. Group seven was non-infected and treated with a full-dose combination of amoxicillin and enrofloxacin. Antimicrobial sensitivity test, minimum inhibitory concentration, minimum bactericidal concentration, and fractional inhibitory concentration were determined. High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combination of enrofloxacin and amoxicillin is safer and more efficient in the treatment of Escherichia coli and Salmonella enteritidis mixed infection in broiler chickens than the usage of these antibiotics in a full-dose combination or solely.
Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal ... more Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal have generated a great deal of interest in recent years, including medical applications. A novel, specific, precise, accurate and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated to quantify copper (Cu) and copper nanoparticles (CuNPs) in different biological matrices and pharmaceutical products. Methods The developed method has been validated for linearity, precision, sensitivity, specificity and accuracy. Cu concentration was detected in pharmaceutical products without an extraction process. Moreover, liver, serum and muscle tissues were used as biological matrices. High Cu recovery in biological samples was afforded by using citric acid as a green chelating agent, exact extraction time and pH adjustment. Cu pharmaceutical and biological samples were eluted by acetonitrile: ammonium acetate (50 mM) with 0...
Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal ... more Background The applications of Cu and CuNPs based on the earth-abundant and inexpensive Cu metal have generated a great deal of interest in recent years, including medical applications. A novel, specific, precise, accurate and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated to quantify copper (Cu) and copper nanoparticles (CuNPs) in different biological matrices and pharmaceutical products. Methods The developed method has been validated for linearity, precision, sensitivity, specificity and accuracy. Cu concentration was detected in pharmaceutical products without an extraction process. Moreover, liver, serum and muscle tissues were used as biological matrices. High Cu recovery in biological samples was afforded by using citric acid as a green chelating agent, exact extraction time and pH adjustment. Cu pharmaceutical and biological samples were eluted by acetonitrile: ammonium acetate (50 mM) with 0...
In this study, the prevalence of Salmonella typhimurium in Egyptian chicken farms was studied. Ex... more In this study, the prevalence of Salmonella typhimurium in Egyptian chicken farms was studied. Examination of 79 chicken farms distributed in Egypt governorates from 2017 to 2019. Salmonella was detected in broiler flocks 20 % positivity (18 out of 90). However, in layer flocks showed 70 % positivity (7 out of 10). Then Salmonella typhimurium was confirmed using Salmonella typhimurium STM4 495 gene using conventional PCR. Lipopolysaccharides (LPSs) are the major constituent of the bacterial Gram-negative outer membrane including Salmonella. (LPSs) is an immunodominant molecule that is important for the virulence and pathogenesis of Salmonella typhimurium which called endotoxin. Extraction and purification of LPS from isolated S. typhimurium using isopropanol and sodium hydroxide mixture this overcome the presence of nucleic acids of extracted Salmonella in the final product. The purity of LPS isolated from Salmonella typhimurium was assessed by HPLC showing highly purified extracted...
The current study was conducted on multiple oral doses (40 mg/kg for 3 successive days) of florfe... more The current study was conducted on multiple oral doses (40 mg/kg for 3 successive days) of florfenicol (FF) to determine its effect on some hematological parameters, cardiac enzymes and its residues in blood and tissues (liver, muscles and kidneys) of broiler chickens. Seventy broiler chickens were used and divided into two groups, each consisted of 35 birds. The first group was left as a control, while the second was given FF for 3 successive days. The FF residues in tissues were determined using reversed phase-high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector at 223 nm. Results indicated a widespread distribution of FF in most of the tested tissues. All tissue samples were considered FF free on the 9 th day after the last oral dose except liver. Florfenicol administration elicited a significant decrease in all blood parameters (hemoglobin concentration (Hb), red blood cell count (RBCs), mean corpuscular volume (MCV), mean corpuscular hemoglobin concen...
An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for... more An accurate, sensitive and reproducible high performance liquid chromatographic (HPLC) method for the quantitation of doxycycline in turkey serum has been developed and validated. The doxycycline standard was eluted from a C18 column (4.6 mm i.d., 250 mm, 5 μm particle size) at 15 0C with a mobile phase consisting of 5% acetic acid : methanol : acetonitrile (55:20:25, v/v/v). A UV detector set at 347 nm was used to monitor the effluent. Retention time of doxycycline was approximately at 4 min and the run time was 10 min. The column temperature adjusted at 15 0C with a flow rate 1ml/min and injection volume 25 μl. Doxycycline was extracted from serum by ACN. Linearity was confirmed in the concentration range 0.1-2 μg/ml, had regression coefficients (R 2 ) 0.9999 for doxycycline quantification in turkey serum. The analytical conditions gave a recoveries in a range of 97.57% to 99.83%. The limit of detection (LOD) was 0.035 μg/ mL and limit of quantification (LOQ) was 0.108 μg/ml. The ...
Effect of extracted Lipopolysaccharides of Salmonella typhimurium on chicken immunity Ashraf A. A... more Effect of extracted Lipopolysaccharides of Salmonella typhimurium on chicken immunity Ashraf A. Abd El-Tawab1, Heba M. Hassan2, Fatma I. El-Hofy1, Mai A. Fadel2, Aml Youssef2, Enas A. Soliman1 Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt. 2 Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center, Dokki, P.O. Box 246, Giza 12618, Egypt
This study aimed to detect and identify the N-acyl-homoserine lactones molecules (AHLs) produced ... more This study aimed to detect and identify the N-acyl-homoserine lactones molecules (AHLs) produced by different resistant Klebsiella pneumoniae isolates recovered from poultry and environmental samples using a modified validated high-performance liquid chromatography method. A total of 56 K. pneumoniae isolates were recovered, investigated for their antibiotic susceptibility, and screened for AHLs production using the Agrobacterium tumefaciens NTL4 biosensor system and a validated high-performance liquid chromatography method. The results revealed the detection of different short- and long-chain AHLs molecules among 39 K. pneumoniae isolates recovered from poultry and environmental samples. All environmental isolates produced nine peaks with retention times for C4-HSL, C6-HSL, C12-HSL, C8-HSL, C14-HSL, C8-oxo-HSL, C10-HSL, C6-oxo-HSL, and C7-HSL. The most quantifiable AHL signal molecules in poultry isolates were C4-HSL, C6-HSL, and C12-HSL. No statistical correlation between the AHL-producing ability of K. pneumoniae isolates and antibiotic resistance was reported. To the best of our knowledge, this study provides the first detailed report on the detection and identification of AHLs in K. pneumoniae isolates recovered from poultry and environmental samples. Furthermore, it provides a new insight available tool other than LC-MS/MS for detection and identification of AHL molecules.
Background and Aim: Lipopolysaccharide (LPS) is an integral part of the outer cell membrane compl... more Background and Aim: Lipopolysaccharide (LPS) is an integral part of the outer cell membrane complex of Gram-negative bacteria. It plays an important role in the induction and stimulation of the immune system. Various LPS purification protocols have been developed. However, analysis of their efficacy is limited by contamination during downstream applications or the public health hazard of LPS. The aim of this study was to evaluate a modified method for extracting LPS as well as assess the purity of the extracted LPS by high-performance liquid chromatography (HPLC) analysis. Further, we evaluated its immunopotentiating function by measuring the relative RNA expression levels of splenic immune-related genes such as interleukin 1β (IL-1β) and interferon-γ (IFN-γ), after intramuscular injection of increasing concentrations of the extracted LPS in specific pathogen-free (SPF) chick. Materials and Methods: Isolation, identification, and serotyping of Salmonella Typhimurium were performed u...
Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, per... more Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, performance, and meat quality while reducing public health concern. Influence of NS and inorganic selenium (Se) water additive on performance, carcass quality, immunoglobulin concentration, intestinal microbiota, Se tissue concentrations, and tissue architecture was studied. Materials and Methods: Two-hundred and sixty 1-day-old Hubbard chicks were randomly grouped into five groups (5×52) and supplemented with 0.5 and 1.0 mL of NS and inorganic Se (100 mg.L-1). G1, G2, G3, and G4 were challenged with Escherichia coli O157: H7 2.6×108 on the 14th day. A total of 2250 samples, including 250 sera, 250 intestinal swabs, and 1500 organ and tissue samples as liver, spleen, heart, bursa, intestine, and breast muscles, and 250 eviscerated carcasses were collected. Results: The results revealed a highly significant increase (p<0.01) in live body weights, weight gains, performance indices, carcass...
Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, per... more Background and Aim: Nano-selenium (NS) supplementation contributes in improving productivity, performance, and meat quality while reducing public health concern. Influence of NS and inorganic selenium (Se) water additive on performance, carcass quality, immunoglobulin concentration, intestinal microbiota, Se tissue concentrations, and tissue architecture was studied. Materials and Methods: Two-hundred and sixty 1-day-old Hubbard chicks were randomly grouped into five groups (5×52) and supplemented with 0.5 and 1.0 mL of NS and inorganic Se (100 mg.L-1). G1, G2, G3, and G4 were challenged with Escherichia coli O157: H7 2.6×108 on the 14th day. A total of 2250 samples, including 250 sera, 250 intestinal swabs, and 1500 organ and tissue samples as liver, spleen, heart, bursa, intestine, and breast muscles, and 250 eviscerated carcasses were collected. Results: The results revealed a highly significant increase (p<0.01) in live body weights, weight gains, performance indices, carcass...
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