Biochimica Et Biophysica Acta-protein Structure and Molecular Enzymology, 1995
The cDNA for coffee bean α-galactosidase (α-Gal) has been cloned and expressed in a baculovirus e... more The cDNA for coffee bean α-galactosidase (α-Gal) has been cloned and expressed in a baculovirus expression system. An early study of coconut α-Gal by chemical modification suggested that one tyrosine residue is at or near the active site. In order to identify such a critical residue, we replaced two tyrosine residues (positions 108 and 158) with phenylalanine by site-directed mutagenesis.
Biochimica Et Biophysica Acta-protein Structure and Molecular Enzymology, 1995
The cDNA for coffee bean α-galactosidase (α-Gal) has been cloned and expressed in a baculovirus e... more The cDNA for coffee bean α-galactosidase (α-Gal) has been cloned and expressed in a baculovirus expression system. An early study of coconut α-Gal by chemical modification suggested that one tyrosine residue is at or near the active site. In order to identify such a critical residue, we replaced two tyrosine residues (positions 108 and 158) with phenylalanine by site-directed mutagenesis.
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Papers by Alex Zhu