Anna Maslennikova
Nizhny novgorod state medical academy, Oncology, Faculty Member
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The aim of this study was to compare the effectiveness of low-intensity LED radiation of the red and infrared spectra for the correction of mucositis in patients receiving radiation and chemoradiation therapy for oral and pharyngeal... more
The aim of this study was to compare the effectiveness of low-intensity LED radiation of the red and infrared spectra for the correction of mucositis in patients receiving radiation and chemoradiation therapy for oral and pharyngeal cancer at an energy density of less than 1 J/cm2. The study included 106 patients who received radiation and chemoradiation therapy for oral cavity and pharyngeal cancer, who were randomly divided into three groups. In the first (37 patients) correction of mucositis was carried out in accordance with the clinic's standards, in the second (36 patients) and the third (33 patients), patients additionally received exposure to the oral cavity with low-intensity LED radiation at a wavelength of 635 nm at a dose of 0,3 J/cm2 (prophylactic regimen) and 0.45 J/cm2 (treatment regimen). When exposed to a wavelength of 780 nm, the dose was 0.6 J/cm2 with a prophylactic regime and 0.8 J/cm2 with a therapeutic regimen, respectively. Exposure to low-level LED irradiation at a wavelength of 635 nm significantly reduced the frequency and severity of radiation mucositis, increased the time until the onset of its first symptoms, reduced the duration of severe mucositis (grade 3) and reduced the patients' need for painkillers, including narcotic analgesics compared with the group receiving standard prophylaxis and correction of mucositis. Photobiomodulation at a wavelength of 780 nm, compared with the standard correction group, significantly increased the time to the onset of the first symptoms of mucositis and decreased the severity of pain.
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For many years, photobiomodulation in cancer patients has been used empirically, based on the positive clinical experience. When using PBM for the prevention and treatment of early radiation toxicity, exposure can occur directly in the... more
For many years, photobiomodulation in cancer patients has been used empirically, based on the positive clinical experience. When using PBM for the prevention and treatment of early radiation toxicity, exposure can occur directly in the area of the tumor site. For that reason, the data is need about a potential influence of low-intensity red light as on the normal as well on the tumor cells exposed to ionizing radiation. The aim of the work was to study the effects of photobiomodulation (PBM) in the red spectrum (640 nm) with fluences from 3 mJ/cm2 to 2 J/cm2 in combination with ionizing radiation at doses of 2–6 Gy against human BJ-5ta-hTERT cells – postnatal fibroblasts. The cells were exposed to low-intensity red light before or after their exposure to IR, the viability of the cells was determined by MTT-test 24 hours after the last exposure. The effects of PBM depend on the fluence of PBM, the dose of IR and the sequence of the actions of these physical factors on cells. The adaptive effect of PBM was observed only for high fluences – 1 and 2 J/cm2 when exposed to PBM and subsequent irradiation of IR. At the same time, the stimulating effect of PBM was observed only for low fluences from 3 to 300 mJ/cm2 under IR irradiation and subsequent (after 1 hour) exposure to PBM. These data should be taken into account when using PBM for the correction of adverse events of radiation therapy in a clinic.
Research Interests:
The aim of the work was studying the effects of photobiomodulation in doses of less than 1 J/cm2 in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before... more
The aim of the work was studying the effects of photobiomodulation in doses of less than 1 J/cm2 in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before and after to gamma-irradiation. Cells viability was determined 24 h after exposure for each gamma-irradiation dose and PBM mode. There was a statistically significant decrease in a number of viable tumor cells for samples that were exposed to PBM prior to gamma-irradiation and a statistically significant increase in a number of viable tumor cells for samples that were exposed to PBM after gamma-irradiation.
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Research Interests: Chemotherapy and Urine
Background. Over the past 20 years, there has been a change in approaches to the treatment of breast cancer, in particular, a significant increase in the role of drug therapy. Breast cancer response to neoadjuvant chemotherapy is... more
Background. Over the past 20 years, there has been a change in approaches to the treatment of breast cancer, in particular, a significant increase in the role of drug therapy. Breast cancer response to neoadjuvant chemotherapy is currently considered as a surrogate biomarker, which allows evaluation of the clinical course and prognosis of the disease. To solve this problem, it is necessary to assess the functional and metabolic changes in tumor tissue during treatment. Doppler ultrasound is a non-invasive, affordable, and low-cost imaging technique that can be safely used for repeated measurements.The purpose of the study was to study vascular changes in the tumor by power Doppler ultrasound for the evaluation of the early breast cancer response to neoadjuvant chemotherapy.Material and Methods. From May 2017 to August 2019, 63 patients with breast cancer received neoadjuvant chemotherapy. Changes in the tumor blood flow were assessed before starting the treatment and prior to the se...
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The possibility of development of noninvasive medicine diagnostics method of pulmonary diseases on the base of exhaled breath analysis with using the nonstationary THz spectroscopy is considered. The results of application of... more
The possibility of development of noninvasive medicine diagnostics method of pulmonary diseases on the base of exhaled breath analysis with using the nonstationary THz spectroscopy is considered. The results of application of nonstationary gas spectroscopy method for monitoring of the radiation therapy of oncological patients are carried out.
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ABSTRACT This paper discusses ways to implement terahertz-range spectrometers based on the effects of freely damped polarization and rapid traverse of the frequency range. It presents the results of using developed spectrometers to record... more
ABSTRACT This paper discusses ways to implement terahertz-range spectrometers based on the effects of freely damped polarization and rapid traverse of the frequency range. It presents the results of using developed spectrometers to record the presence of ammonia, acetone, and nitric oxide in exhaled air, as well as to analyze a liquid (Custodiol) for flushing transplanted parenchymatous organs.
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Effective breast cancer treatment requires the assessment of metabolic changes of tumor tissue during chemo- and hormonotherapy for prediction tumor response. Evaluation of the dynamics of tumor oxygen state (by diffuse optical... more
Effective breast cancer treatment requires the assessment of metabolic changes of tumor tissue during chemo- and hormonotherapy for prediction tumor response. Evaluation of the dynamics of tumor oxygen state (by diffuse optical spectroscopy imaging) and tumor vasculature (by ultrasound investigation in power Doppler mode) was performed before treatment beginning and before the second cycle of chemotherapy in 16 patients who received preoperative chemotherapy. Changes of these indicators were compared then with tumor pathologic response. Breast tumors demonstrated different dynamics of tumor oxygenation depending on the changes of tumor tissue. The increase of the tumor oxygenation after the first cycle of chemotherapy was observed in five of six patients with grade 4 and 5 of pathologic tumor response. Decrease of the oxygenation level was revealed in one patient with the 4th degree of tumor response. Variable changes of the oxygenation level were mentioned in the patients with moderate (the 3d degree) tumor response. Tumor oxygenation decreased or was unchanged in case of 1 or 2 degree of tumor response in five of six cases. The study of the tumor blood vessels didn't reveal any correlation between vasculature changes and tumor response under the performed treatment. The trend of tumor oxygenation in early time after treatment beginning might be a predictive criterion of tumor sensitivity to chemotherapy.
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Dynamics of breast tumor’s oxygenation and tumor blood flow during neoadjuvant chemotherapy was determined by diffuse optical spectroscopy and ultrasound investigation. Oxygenation and blood flow dynamics demonstrated variable changes... more
Dynamics of breast tumor’s oxygenation and tumor blood flow during neoadjuvant chemotherapy was determined by diffuse optical spectroscopy and ultrasound investigation. Oxygenation and blood flow dynamics demonstrated variable changes depending on tumor response.
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The paper presents the results from a study of the breast oxygen status by diffuse optical tomography (DOT). Detection of breast tissue at wavelengths of 684, 794, and 850 nm could provide information on the distribution of basic tissue... more
The paper presents the results from a study of the breast oxygen status by diffuse optical tomography (DOT). Detection of breast tissue at wavelengths of 684, 794, and 850 nm could provide information on the distribution of basic tissue chromophores: oxygenized and deoxygenized hemoglobin. Normal breast tissue was characterized by the even distribution of these compounds and stabilization of their level. In breast cancer, the distribution of oxyand deoxyhemoglobin was noted for uneven distribution and blood oxygen saturation was lower in the projection of a tumor nodule. The blood oxygen saturation data obtained by DOT demonstrate physiological differences between normal and tumor tissues in different tumor areas.
Bladder cancer, occurring at different times after radiation therapy for tumors of the pelvic organs (cervical cancer, uterus cancer, prostate cancer) is a specific problem of oncourology. The main visual manifestations of the adverse... more
Bladder cancer, occurring at different times after radiation therapy for tumors of the pelvic organs (cervical cancer, uterus cancer, prostate cancer) is a specific problem of oncourology. The main visual manifestations of the adverse events of radiation therapy in early time after irradiation are pronounced edema and hyperemia of the bladder mucosa, in the case of severe complications – hemorrhages. In years after radiation exposure atrophic changes of the mucous membrane, telangiectasia and reduction of the capacity of the bladder occur; late complications of a severe degree manifest as ulcers and fistulas. The main clinical symptoms of bladder cancer are hematuria and dysuria. The cystoscopy still remains the “gold standard” for its diagnosis, but this method appears to have significant limitations if a tumor arises in bladder tissue suffering from radiation exposure. In this case, the clinical symptoms and the cystoscopic picture can be regarded as manifestations of the side effects of RT, especially in case of grade 3 and 4 of complication. Optical methods may play a key role for distinguishing between a metachronous bladder cancer and severe complications of radiation therapy. Optical coherence tomography (OCT) has a spatial resolution corresponding to several micrometers and makes it possible to assess the structure of biological tissues at a depth of up to 2 mm. Besides, polarization-sensitive methods OCT (CP OCT) allow evaluating the state of the connective tissue matrix which loses orderliness and organized structure of the arrangement of collagen fibers and bundles in malignant tumors. The study presents the case reports of bladder cancer which arose against the radiation-induced changes of bladder tissue after previous irradiation for cervical cancer and were diagnosed by optical coherence tomography.
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The aim of the investigation was evaluating the possibilities of cross-polarization optical coherence tomography (CP OCT) to monitor the effectiveness of photodynamic therapy (PDT) and radiation therapy (RT) of the oral cancer and of the... more
The aim of the investigation was evaluating the possibilities of cross-polarization optical coherence tomography (CP OCT) to monitor the effectiveness of photodynamic therapy (PDT) and radiation therapy (RT) of the oral cancer and of the cervical cancer. Materials and Methods. The CP OCT investigation was performed by an OCT-1300U tomograph (Institute of Applied Physics of the Russian Academy of Sciences, LLC “Biomedtech”, Russia). The monitoring of the two types of treatment was performed after different periods of exposure: for RT of the oral cancer — once for every 2–3 days throughout the whole course of the treatment, starting from the first day of radiation; for the PDT of the cancers of the skin and cervix uteri — before the exposure, immediately after the exposure, and then at 1 day, 7 day and 1 month after the exposure. Fourteen patients were examined in total. Results. The results of the study of the medical pathomorphism of different kinds of tumors performed on the 14 pat...
AIM To investigate the role of structural changes of the urinary bladder extracellular matrix in the occurrence of different grades of adverse events after radiation therapy. MATERIALS AND METHODS The connective tissue matrix was studied... more
AIM To investigate the role of structural changes of the urinary bladder extracellular matrix in the occurrence of different grades of adverse events after radiation therapy. MATERIALS AND METHODS The connective tissue matrix was studied using 126 images obtained from the histological sections of the bladder biopsy specimens of 12 patients classified according to the clinical presentation and the grades of late bladder toxicity according to RTOG/EORTC Late Radiation Morbidity Scoring Scheme. Control images of the normal bladder (n=23) were collected from the autopsy samples. We used nonlinear microscopy imaging method capturing the second harmonic generation (SHG) signal and two-photon excitation auto-fluorescence (TPEA). RESULTS The findings of nonlinear microscopy of urinary bladder histological sections showed that the structural changes in the connective tissue differed depending on the grade of adverse events: grade II adverse events were associated with the preservation of the...
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The aim of the investigation is complex study of the mechanisms of radiation-induced collagen damage and following recovery of its structure on various levels of multilayer organization. Materials and Methods. Rat tail tendons were used... more
The aim of the investigation is complex study of the mechanisms of radiation-induced collagen damage and following recovery of its structure on various levels of multilayer organization. Materials and Methods. Rat tail tendons were used as the model of collagen containing tissue. The animals were exposed to radiation at the dosage of 2, 4, 6, 8, and 10 Gy using gamma-apparatus Luch-1 (Russia) in vivo. The collagen structure was studied 1 and 7 days after the radiation by trypsin-resistance test, differential scanning calorimetry, confocal microscopy with second-harmonic generation imaging, and cross polarization optical coherence tomography. Results. There were determined certain regularities of collagen structural changes depending on radiation time. One day after the radiation of collagen containing tissue the leading processes were those of collagen structural damage at molecular level due to reactive oxygen species exposure. Within a week after the radiation, the collagen struct...
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Breast cancer (BC) is one of the most common malignancies and the leading cause of cancer-related death in women worldwide. Currently, mammography and ultrasound are the methods most commonly used for the detection of BC, but these... more
Breast cancer (BC) is one of the most common malignancies and the leading cause of cancer-related death in women worldwide. Currently, mammography and ultrasound are the methods most commonly used for the detection of BC, but these methods have significant limitations in the absence of “classic” visual symptoms of cancer in the breast tissue. In the complicated clinical situations, such methods as magnetic resonance imaging and positron emission tomography are used for the differential diagnosis of breast cancer. Over the past 20 years, the BC imaging capabilities have expanded due to the introduction of optical methods into clinical practice. These methods are based on differences in the optical properties between normal and pathological breast tissues. Optical imaging provides the ability to obtain indirect information about oxygenation, blood supply, delivery and consumption of oxygen in the breast tissue, as well as changes in its scattering properties. We present a clinical cas...
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Mucositis is the limiting toxicity of radio(chemo)therapy of head and neck cancer. Diagnostics, prophylaxis and correction of this condition demand new accurate and objective approaches. Here we report on an in vivo longitudinal... more
Mucositis is the limiting toxicity of radio(chemo)therapy of head and neck cancer. Diagnostics, prophylaxis and correction of this condition demand new accurate and objective approaches. Here we report on an in vivo longitudinal monitoring of the oral mucosa dynamics in 25 patients during the course of radiotherapy of oropharyngeal and nasopharyngeal cancer using multifunctional optical coherence tomography (OCT). A spectral domain OCT system with a specially-designed oral imaging probe was used. Microvasculature visualization was based on temporal speckle variations of the full complex signal evaluated by high-pass filtering of 3D data along the slow scan axis. Angiographic image quantification demonstrated an increase of the vascular density and total length of capillary-like-vessels before visual signs or clinical symptoms of mucositis occur. Especially significant microvascular changes compared to their initial levels occurred when grade two and three mucositis developed. Furthe...
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Background Breast cancer neoadjuvant chemotherapy (NACT) allows assessing tumor sensitivity to systemic treatment, planning adjuvant treatment and follow-up. However, a sufficiently large number of patients fail to achieve the desired... more
Background Breast cancer neoadjuvant chemotherapy (NACT) allows assessing tumor sensitivity to systemic treatment, planning adjuvant treatment and follow-up. However, a sufficiently large number of patients fail to achieve the desired level of pathological tumor response while optimal early response assessment methods have not been established now. In our study, we simultaneously assessed the early chemotherapy-induced changes in the tumor volume by Ultrasound (US), the tumor oxygenation by Diffuse Optical Spectroscopy Imaging (DOSI) and the state of the tumor vascular bed by Doppler US to elaborate the predictive criteria of breast tumor response to treatment. Methods A total of 133 patients with a confirmed diagnosis of invasive breast cancer stage II to III admitted to NACT with following definitive breast surgery were enrolled, 108 of them completed the therapy. Tumor oxygenation by DOSI, tumor volume by US and tumor vascularization by Doppler US were determined before the first...
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For many years, photobiomodulation in cancer patients has been used empirically, based on the positive clinical experience. When using PBM for the prevention and treatment of early radiation toxicity, exposure can occur directly in the... more
For many years, photobiomodulation in cancer patients has been used empirically, based on the positive clinical experience. When using PBM for the prevention and treatment of early radiation toxicity, exposure can occur directly in the area of the tumor site. For that reason, the data is need about a potential influence of low-intensity red light as on the normal as well on the tumor cells exposed to ionizing radiation. The aim of the work was to study the effects of photobiomodulation (PBM) in the red spectrum (640 nm) with fluences from 3 mJ/cm2 to 2 J/cm2 in combination with ionizing radiation at doses of 2–6 Gy against human BJ-5ta-hTERT cells – postnatal fibroblasts. The cells were exposed to low-intensity red light before or after their exposure to IR, the viability of the cells was determined by MTT-test 24 hours after the last exposure. The effects of PBM depend on the fluence of PBM, the dose of IR and the sequence of the actions of these physical factors on cells. The adaptive effect of PBM was observed only for high fluences – 1 and 2 J/cm2 when exposed to PBM and subsequent irradiation of IR. At the same time, the stimulating effect of PBM was observed only for low fluences from 3 to 300 mJ/cm2 under IR irradiation and subsequent (after 1 hour) exposure to PBM. These data should be taken into account when using PBM for the correction of adverse events of radiation therapy in a clinic.
Research Interests:
The aim of the work was studying the effects of photobiomodulation in doses of less than 1 J/cm2 in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before... more
The aim of the work was studying the effects of photobiomodulation in doses of less than 1 J/cm2 in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before and after to gamma-irradiation. Cells viability was determined 24 h after exposure for each gamma-irradiation dose and PBM mode. There was a statistically significant decrease in a number of viable tumor cells for samples that were exposed to PBM prior to gamma-irradiation and a statistically significant increase in a number of viable tumor cells for samples that were exposed to PBM after gamma-irradiation.
Research Interests:
The aim of this study was to compare the effectiveness of low-intensity LED radiation of the red and infrared spectra for the correction of mucositis in patients receiving radiation and chemoradiation therapy for oral and pharyngeal... more
The aim of this study was to compare the effectiveness of low-intensity LED radiation of the red and infrared spectra for the correction of mucositis in patients receiving radiation and chemoradiation therapy for oral and pharyngeal cancer at an energy density of less than 1 J/cm2. The study included 106 patients who received radiation and chemoradiation therapy for oral cavity and pharyngeal cancer, who were randomly divided into three groups. In the first (37 patients) correction of mucositis was carried out in accordance with the clinic's standards, in the second (36 patients) and the third (33 patients), patients additionally received exposure to the oral cavity with low-intensity LED radiation at a wavelength of 635 nm at a dose of 0,3 J/cm2 (prophylactic regimen) and 0.45 J/cm2 (treatment regimen). When exposed to a wavelength of 780 nm, the dose was 0.6 J/cm2 with a prophylactic regime and 0.8 J/cm2 with a therapeutic regimen, respectively. Exposure to low-level LED irrad...
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Using optoacoustic microscopy, a radiation-induced increase in the fragmentation of experimental tumor small vessels, as well as the formation of large hemoglobin-containing structures were revealed within first days after treatment.
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Development of a number of diseases is accompanied by changes in the blood vessels’ structure and the investigation of tissue vascular pattern remains one of the most essential problems in experimental and clinical medicine. Hybrid... more
Development of a number of diseases is accompanied by changes in the blood vessels’ structure and the investigation of tissue vascular pattern remains one of the most essential problems in experimental and clinical medicine. Hybrid methods of optoacoustic (OA) imaging enable label-free optical-contrast angiography at optical penetration depths with ultrasonic resolution. We used OA to study vascular network of experimental tumor during growth and after treatment as well as to study the dynamics of blood content of human skin during functional tests. Optoacoustic angiography was performed using raster-scan system in reflection mode with 532 nm laser source and wideband PVDF detector. The sensitivity of the system allowed to visualize 50 µm blood vessels at up to 2.1 mm depth. Minimally detected diameter of blood vessel located at the surface of a soft tissue was 15 µm. Imaging of colon tumor models CT26 and HT-29 revealed peculiarities of vascular system development. Irradiation-indu...
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Identifying the precise topography of cancer for targeted biopsy in colonoscopic examination is a challenge in current diagnostic practice. For the first time we demonstrate the use of compression optical coherence elastography (C-OCE)... more
Identifying the precise topography of cancer for targeted biopsy in colonoscopic examination is a challenge in current diagnostic practice. For the first time we demonstrate the use of compression optical coherence elastography (C-OCE) technology as a new functional OCT modality for differentiating between cancerous and non-cancerous tissues in colon and detecting their morphological features on the basis of measurement of tissue elastic properties. The method uses pre-determined stiffness values (Young’s modulus) to distinguish between different morphological structures of normal (mucosa and submucosa), benign tumor (adenoma) and malignant tumor tissue (including cancer cells, gland-like structures, cribriform gland-like structures, stromal fibers, extracellular mucin). After analyzing in excess of fifty tissue samples, a threshold stiffness value of 520 kPa was suggested above which areas of colorectal cancer were detected invariably. A high Pearson correlation (r =0.98; p <0.0...
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The research is devoted to comparison of the blood vessel structure and the oxygen state of three xenografts: SN-12C, HCT-116 and Colo320. Differences in the vessel formation and the level of oxygenation are revealed by optoacoustic (OA)... more
The research is devoted to comparison of the blood vessel structure and the oxygen state of three xenografts: SN-12C, HCT-116 and Colo320. Differences in the vessel formation and the level of oxygenation are revealed by optoacoustic (OA) microscopy and diffuse optical spectroscopy (DOS) respectively. The Colo320 tumor is characterized by the highest values of vessel size and fraction. DOS showed increased content of deoxyhemoglobin that led to reduction of saturation level for Colo320 as compared to other tumors. Immunohistochemical (IHC) analysis for CD31 demonstrates the higher number of vessels in Colo320. The IHC for hypoxia was consistent with DOS results and revealed higher values of the relative hypoxic fraction in Colo320.
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A THz nonstationary high-resolution spectrometer based on semiconductor superlattice multipliers is applied to investigate the dynamics of urine composition for cancer patients treated with chemotherapy. The molecular urine composition of... more
A THz nonstationary high-resolution spectrometer based on semiconductor superlattice multipliers is applied to investigate the dynamics of urine composition for cancer patients treated with chemotherapy. The molecular urine composition of healthy volunteers and cancer patients was compared and contrasted. We have found a set of nitriles that either appeared after chemotherapy or increased in content, which are expected as a result of bio-chemical damage to the liver. While no damage can be detected at this stage by existing clinical methods, the identified nitriles are candidates for further large-scale systematic testing towards markers for nephrotoxicity of chemotherapy at an early stage of the treatment, when conventional diagnostics cannot identify substantial organ damage. Comparing the metabolite concentration dynamics with side effects during chemotherapy might then help individuate patients prone to severe complications and correct the treatment. Our devices are game-changer...
Research Interests: Chemotherapy and Urine
The possibilities of optoacoustic microscopy for comparison of vascular network of different tumor models as well as for investigation of tumor vessels response to radiation therapy were demonstrated.
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The aim of the work was studying the effects of photobiomodulation of a red spectrum in doses of less than 1 J/cm2 in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy... more
The aim of the work was studying the effects of photobiomodulation of a red spectrum in doses of less than 1 J/cm2 in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before and after to gamma-irradiation. Cells viability was determined 24 h after exposure for each gamma-irradiation dose and each PBM mode. There was a statistically significant decrease in a number of viable tumor cells for the samples that were exposed to low-intensity red light prior to gamma-irradiation and a statistically significant increase in a number of viable tumor cells for the samples that were exposed to low-intensity red light after gamma-irradiation. An increase in the number of viable tumor cells exposed to PBM after gamma irradiation correlates with a decrease in the number of cells with a depolarized mitochondrial membrane. The results of a current study need to take into consideration at further studies of PBM effects o...